Biochemical Analysis Tools - Methods for Bio-Molecules Studies最新文献

The Chemistry Behind Plant DNA Isolation Protocols 植物DNA分离协议背后的化学原理

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2020-05-19 DOI: 10.5772/intechopen.92206

Jina Heikrujam, R. Kishor, P. Mazumder

引用次数: 16

Latest Implications of Next-Gen Sequencing in Diagnosis of Acute and Chronic Myeloid Leukemia 新一代测序在急性和慢性髓系白血病诊断中的最新意义

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2020-04-08 DOI: 10.5772/intechopen.92068

O. Boldura, Cristina Petrine, A. Mihu, C. Baltă

引用次数: 0

Detection of the Species Composition of Food Using Mitochondrial DNA: Challenges and Possibilities of a Modern Laboratory 利用线粒体DNA检测食物的物种组成:现代实验室的挑战和可能性

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-11-27 DOI: 10.5772/INTECHOPEN.89579

M. Natonek-Wiśniewska, P. Krzyścin

{"title":"Detection of the Species Composition of Food Using Mitochondrial DNA: Challenges and Possibilities of a Modern Laboratory","authors":"M. Natonek-Wiśniewska, P. Krzyścin","doi":"10.5772/INTECHOPEN.89579","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.89579","url":null,"abstract":"Monitoring food quality is an important and constant element of the food market. This need is connected with health issues, religious beliefs of consumers, and economic considerations. For analysis, mtDNA is most commonly used because it is resist to physical factors such as temperature and pressure, which very often accompany food processing. Nowadays, scientific publications present a number of methods describing species identification from both farm animals and also less common animals. The most effective methods for determining species are based on PCR, real-time PCR, and sequencing. The methods are very sensitive, limit of detection (LOD) is 0.001% for many of them. An indispensable element of per-forming the described research is the strict application in the laboratory of several principles, which are intended to improve the work and make it safe for the lab technician, as well as guarantee the quality and effectiveness of the experiments carried out. The high work requirements set for the crew naturally shape the quality system from which the most popular is ISO/IEC 17025. Modern methods based on mtDNA are a good tool for food analysis, creating great opportunities for the researcher, at the same time causing challenges for the contemporary laboratory.","PeriodicalId":105987,"journal":{"name":"Biochemical Analysis Tools - Methods for Bio-Molecules Studies","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124982476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Evidence Analysis in Cases of Sexual Assault 性侵案件的生物学证据分析

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-11-27 DOI: 10.5772/INTECHOPEN.82164

Benito Ramos González, Miranda Córdova Mercado, Orlando Salas Salas, J. Reyes, Martín Guardiola Ramos, Elton Solís Esquivel, Gerardo Aguilar, Porfirio Diaz Torres

{"title":"Biological Evidence Analysis in Cases of Sexual Assault","authors":"Benito Ramos González, Miranda Córdova Mercado, Orlando Salas Salas, J. Reyes, Martín Guardiola Ramos, Elton Solís Esquivel, Gerardo Aguilar, Porfirio Diaz Torres","doi":"10.5772/INTECHOPEN.82164","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.82164","url":null,"abstract":"Sexual assault (SA) is a crime of violence against a person’s body resulting in a physical trauma, mental anguish, and suffering for victims generating expenses for government intended criminal investigation, medical care, and psychological attention. During the crime scene investigation, the identification and recovery of biological evidence (BE) are utmost important, since sometimes these are the only way to prove sexual contact and the perpetrator’s identity. The examiner, with the help of specific technologies and techniques, must be able to find evidence that oth-erwise could go unnoticed. Forensic laboratories identify biological evidence with systemized protocols and use molecular methods to generate DNA profiles based on the amplification and DNA sequencing. Before the arrival of the new-generation sequencers, the application of other markers (single nucleotide polymorphisms (SNPs), insertion-deletion of nucleotides (INDEL), or microhaplotypes (MHs)) was laborious, expensive, and not very informative for forensic purposes; however, now they are useful in this field. Next-generation sequencing (NGS) brought a new series of applications like epigenetics, microbiota, messenger RNA, and microRNA analysis and the inferences in the ancestry and phenotyping of individuals. In the end, the results obtained from such analyses and stored in databases are very useful for the identification of sexual aggressors.","PeriodicalId":105987,"journal":{"name":"Biochemical Analysis Tools - Methods for Bio-Molecules Studies","volume":"315 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126521711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Principles of Chromatography Method Development 色谱法发展原理“，

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-10-26 DOI: 10.5772/intechopen.89501

Dr. Narasimha S. Lakka, PhD, C. Kuppan

引用次数: 15

Molecular Tools for Gene Analysis in Fission Yeast 裂变酵母基因分析的分子工具

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-10-25 DOI: 10.5772/intechopen.84896

I. Herrera-Camacho, L. Millan-PerezPeña, Francisca Sosa-Jurado, N. Martínez-Montiel, Rebeca D Martínez-Contreras, Nora Hilda Rosas Murrieta

引用次数: 0

DNA Sequencing Resolves Misdiagnosed and Rare Genetic Disorders DNA测序解决误诊和罕见的遗传疾病

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-09-30 DOI: 10.5772/intechopen.86556

Alice Abdel Aleem

{"title":"DNA Sequencing Resolves Misdiagnosed and Rare Genetic Disorders","authors":"Alice Abdel Aleem","doi":"10.5772/intechopen.86556","DOIUrl":"https://doi.org/10.5772/intechopen.86556","url":null,"abstract":"This chapter focuses on the mandatory requirement of DNA sequencing approaches for genetic diagnosis and recurrence prevention of inherited diseases. Sequencing the DNA and coded transcripts has intensely promoted our understanding of functional genomics and the fundamental importance of non-coding genomic sequences in causing heritable diseases, when mutated. Though Sanger sequencing, the first employed approach in identifying genetic mutations has been replaced nowa-days in many laboratories with the highly robust massive parallel sequencing tech-niques, “Sanger” remains vital in countries with limited resources and also of essential importance in validating the results of large scale sequencing technologies. Next generation sequencing (NGS) enabled the parallel sequencing of the whole exome (WES) and whole genome (WGS) regions of human genome and has revolutionized the field of genetic and genomic research in human. WES and WGS have facilitated the identification of the role of previously unrecognized genes in causing neurologic phenotypes, brain structural malformation, and resolved the causal genes in puzzling and misdiagnosed genetic phenotypes. Role of fusion genes and non-coding RNA in causing neurogenetic recessive diseases has been uncovered by the application of NGS platforms, published examples are presented in this chapter. Extensive phenotypic variability that retained patients either as misdiagnosed or undiagnosed cases for years has been correctly diagnosed through NGS research applications.","PeriodicalId":105987,"journal":{"name":"Biochemical Analysis Tools - Methods for Bio-Molecules Studies","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114793306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Markers and Their Optimization: Addressing the Problems of Nonhomology Using Decapod COI Gene 分子标记及其优化:解决十足COI基因的非同源性问题

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-09-06 DOI: 10.5772/intechopen.86993

Deepak Jose, M. Harikrishnan

{"title":"Molecular Markers and Their Optimization: Addressing the Problems of Nonhomology Using Decapod COI Gene","authors":"Deepak Jose, M. Harikrishnan","doi":"10.5772/intechopen.86993","DOIUrl":"https://doi.org/10.5772/intechopen.86993","url":null,"abstract":"Advancements in DNA sequencing and computational technologies influenced almost all areas of biological sciences. DNA barcoding technology employed for generating nucleotide sequences (DNA barcodes) from standard gene region(s) is capable of resolving the complexities caused due to morphological characters. Thus, they complement taxonomy, population analysis, and phylogenetic and evolutionary studies. DNA barcodes are also utilized for species identification from eggs, larvae, and commercial products. Sequence similarity search using Basic Local Alignment Search Tool (BLAST) is the most reliable and widely used strategy for characterizing newly generated sequences. Similarity searches identify “homologous” gene sequence(s) for query sequence(s) by statistical calculations and provide identity scores. However, DNA barcoding relies on diverse DNA regions which differ considerably among taxa. Even, region-specific variations within barcode sequences from a single gene leading to “nonhomology” have been reported. This causes complications in specimen identification, population analysis, phylogeny, evolution, and allied studies. Hence, the selection of appropriate barcode region(s) homologous to organism of interest is inevitable. Such complications could be avoided using standardized barcode regions sequenced using optimized primers. This chapter discusses about the potential problems encountered due to the unknown/unintentional/intentional use of nonhomologous barcode regions and the need for primer optimization.","PeriodicalId":105987,"journal":{"name":"Biochemical Analysis Tools - Methods for Bio-Molecules Studies","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115304270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Online Automated Micro Sample Preparation for High-Performance Liquid Chromatography 高效液相色谱在线自动化微样品制备

Biochemical Analysis Tools - Methods for Bio-Molecules Studies Pub Date : 2019-08-29 DOI: 10.5772/intechopen.89079

H. Kataoka, A. Ishizaki, Keita Saito

{"title":"Online Automated Micro Sample Preparation for High-Performance Liquid Chromatography","authors":"H. Kataoka, A. Ishizaki, Keita Saito","doi":"10.5772/intechopen.89079","DOIUrl":"https://doi.org/10.5772/intechopen.89079","url":null,"abstract":"Sample preparation is one of the most labor-intensive and time-consuming operations in sample analysis. Sample preparation strategies include the exhaustive or non-exhaustive extraction of analytes from matrices. Online coupling of sample preparation with the separation system is regarded as an important goal. In-tube solid-phase microextraction (SPME) is an effective sample preparation technique that uses an open tubular fused-silica capillary column as an extraction device. In-tube SPME is useful for trace enrichment, automated sample cleanup, and rapid online analysis. Moreover, this method can be used to determine the analytes in complex matrices by direct sample injection or merely by simple sample treatment such as filtration. In-tube SPME is frequently combined with high-performance liquid chromatography (HPLC) using online column-switching techniques. Various operating systems and new sorbent materials have been reported to improve extraction efficiency, such as sorption capacity and selectivity. This chapter discusses efficient micro sample preparation techniques for HPLC, especially online automated in-tube SPME.","PeriodicalId":105987,"journal":{"name":"Biochemical Analysis Tools - Methods for Bio-Molecules Studies","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125059726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1