Light in Nature VII最新文献

筛选
英文 中文
Fluorescence lifetime-based intrinsic metabolic signatures of microglia cell (Conference Presentation) 基于荧光寿命的小胶质细胞内在代谢特征(会议报告)
Light in Nature VII Pub Date : 2019-09-10 DOI: 10.1117/12.2529349
A. K. Sagar, Kevin P. Cheng, Jonathan N. Ouellette, Justin C. Williams, J. Watters, K. Eliceiri
{"title":"Fluorescence lifetime-based intrinsic metabolic signatures of microglia cell (Conference Presentation)","authors":"A. K. Sagar, Kevin P. Cheng, Jonathan N. Ouellette, Justin C. Williams, J. Watters, K. Eliceiri","doi":"10.1117/12.2529349","DOIUrl":"https://doi.org/10.1117/12.2529349","url":null,"abstract":"Microglia are central nervous system (CNS) resident macrophages. They play a prominent role in virtually all neurodegenerative and traumatic brain injuries. Visualizing microglia using label-free methodologies will allow a better understanding of how microglia participate in CNS disorders in the absence of perturbations from external fluorescent dyes. Fluorescence Lifetime Imaging (FLIM) of NADH is an effective tool for monitoring cell intrinsic metabolic changes, and can imply metabolic state based on free/bound NADH lifetime quantification. Recently, FLIM based NADH lifetime quantification was used to characterize macrophages and other immune cell types. Here, we use a lifetime-based, label-free method to characterize microglia in vitro. We have found that there is a unique and statistically significant difference (p<0.05, n=5) in the NADH lifetime and free/bound NADH levels in microglia compared to other CNS cell types. Activated (i.e. inflammatory) microglia play a particularly important role in CNS diseases compared to quiescent microglia, and to our knowledge, there are no markers that can uniquely identify activated microglia, and distinguish them from related immune cell types. Thus, we have extended our NADH FLIM-based approach to differentiate quiescent microglia from activated microglia and found that there is a statistically significant difference (p<0.05,n=5) in NADH mean lifetime in the activated cells. Identifying microglia in a mixed population of CNS cells, and distinguishing activated from quiescent microglia will pave the way to better understanding their roles in the healthy and diseased brain.","PeriodicalId":103401,"journal":{"name":"Light in Nature VII","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117322650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信