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Sensitive Analysis of Triiodothyronine in Serum by One-Step Multiple Methylations Followed by LC/ESI-MS/MS 用 LC/ESI-MS/MS 对血清中的三碘甲状腺原氨酸进行一步多重甲基化灵敏分析
IF 1.7
Chromatography Pub Date : 2024-04-26 DOI: 10.15583/jpchrom.2024.004
Hiroki Matsuoka, Honoka Kaneko, Takumi Narita, Takayuki Ishige, Tatsuya Higashi
{"title":"Sensitive Analysis of Triiodothyronine in Serum by One-Step Multiple Methylations Followed by LC/ESI-MS/MS","authors":"Hiroki Matsuoka, Honoka Kaneko, Takumi Narita, Takayuki Ishige, Tatsuya Higashi","doi":"10.15583/jpchrom.2024.004","DOIUrl":"https://doi.org/10.15583/jpchrom.2024.004","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140652946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adsorption of Gaseous Organic Compounds onto PM2.5 During Air Sampling 空气采样过程中 PM2.5 对气态有机化合物的吸附情况
IF 1.7
Chromatography Pub Date : 2024-04-05 DOI: 10.15583/jpchrom.2024.002
I. Ueta, Hyuma Suzuki, T. Koyama, Yoshihiro Saito
{"title":"Adsorption of Gaseous Organic Compounds onto PM2.5 During Air Sampling","authors":"I. Ueta, Hyuma Suzuki, T. Koyama, Yoshihiro Saito","doi":"10.15583/jpchrom.2024.002","DOIUrl":"https://doi.org/10.15583/jpchrom.2024.002","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140740404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Two-Dimensional LC-MS/MS Methods for Highly-Selective Analysis of Chiral Amino Acids and the Evaluation of Their Intrinsic Amounts in Mammals and Stereoinversion in Proteins 开发用于高选择性分析手性氨基酸的二维 LC-MS/MS 方法，并评估其在哺乳动物体内的固有数量和蛋白质中的立体反转情况
IF 1.7
Chromatography Pub Date : 2024-02-02 DOI: 10.15583/jpchrom.2024.001
Chiharu Ishii, K. Hamase
{"title":"Development of Two-Dimensional LC-MS/MS Methods for Highly-Selective Analysis of Chiral Amino Acids and the Evaluation of Their Intrinsic Amounts in Mammals and Stereoinversion in Proteins","authors":"Chiharu Ishii, K. Hamase","doi":"10.15583/jpchrom.2024.001","DOIUrl":"https://doi.org/10.15583/jpchrom.2024.001","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139809507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Two-Dimensional LC-MS/MS Methods for Highly-Selective Analysis of Chiral Amino Acids and the Evaluation of Their Intrinsic Amounts in Mammals and Stereoinversion in Proteins 开发用于高选择性分析手性氨基酸的二维 LC-MS/MS 方法，并评估其在哺乳动物体内的固有数量和蛋白质中的立体反转情况
IF 1.7
Chromatography Pub Date : 2024-02-02 DOI: 10.15583/jpchrom.2024.001
Chiharu Ishii, K. Hamase
{"title":"Development of Two-Dimensional LC-MS/MS Methods for Highly-Selective Analysis of Chiral Amino Acids and the Evaluation of Their Intrinsic Amounts in Mammals and Stereoinversion in Proteins","authors":"Chiharu Ishii, K. Hamase","doi":"10.15583/jpchrom.2024.001","DOIUrl":"https://doi.org/10.15583/jpchrom.2024.001","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139869399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Novel Separation and Analytical Methods for Optically Active Substances and Therapeutic Monoclonal Antibodies Based on Liquid Chromatography 基于液相色谱法开发光学活性物质和治疗性单克隆抗体的新型分离和分析方法
IF 1.7
Chromatography Pub Date : 2024-01-23 DOI: 10.15583/jpchrom.2023.024
K. Todoroki
{"title":"Development of Novel Separation and Analytical Methods for Optically Active Substances and Therapeutic Monoclonal Antibodies Based on Liquid Chromatography","authors":"K. Todoroki","doi":"10.15583/jpchrom.2023.024","DOIUrl":"https://doi.org/10.15583/jpchrom.2023.024","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139604530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Cryogenic HPLC for Wide Range Low Temperatures Using Liquefied Propane as Mobile Phase and Its Application to Hydrogen/Deuterium Compounds Separation 以液化丙烷为流动相的宽范围低温高效液相色谱的开发及其在氢/氘化合物分离中的应用
IF 1.7
Chromatography Pub Date : 2023-12-31 DOI: 10.15583/jpchrom.2023.018
Masahiro Hattori, Hiroki Iwase, S. Kitagawa, Yoshinori Iiguni, Hajime Ohtani
{"title":"Development of Cryogenic HPLC for Wide Range Low Temperatures Using Liquefied Propane as Mobile Phase and Its Application to Hydrogen/Deuterium Compounds Separation","authors":"Masahiro Hattori, Hiroki Iwase, S. Kitagawa, Yoshinori Iiguni, Hajime Ohtani","doi":"10.15583/jpchrom.2023.018","DOIUrl":"https://doi.org/10.15583/jpchrom.2023.018","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139134028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Capillary Electrophoretic Characterization of Platinum and Silver Nanoparticles in Aqueous Solution Prepared by a Solution Plasma Process 利用溶液等离子工艺制备水溶液中的铂和银纳米粒子的毛细管电泳特性分析
IF 1.7
Chromatography Pub Date : 2023-12-13 DOI: 10.15583/jpchrom.2023.022
Toshio Takayanagi, Taiki Hirose, Hitoshi Mizuguchi, Hirotaka Okabe, Naoki Matsuda
{"title":"Capillary Electrophoretic Characterization of Platinum and Silver Nanoparticles in Aqueous Solution Prepared by a Solution Plasma Process","authors":"Toshio Takayanagi, Taiki Hirose, Hitoshi Mizuguchi, Hirotaka Okabe, Naoki Matsuda","doi":"10.15583/jpchrom.2023.022","DOIUrl":"https://doi.org/10.15583/jpchrom.2023.022","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139006144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amino Acid Analysis in Rat Wound Exudate by High-Performance Liquid Chromatography-Fluorescence Detection 用高效液相色谱-荧光检测法分析大鼠伤口渗出液中的氨基酸
IF 1.7
Chromatography Pub Date : 2023-12-04 DOI: 10.15583/jpchrom.2023.021
Makoto Tsunoda, Yuma Ishida, Mao Kunimitsu
{"title":"Amino Acid Analysis in Rat Wound Exudate by High-Performance Liquid Chromatography-Fluorescence Detection","authors":"Makoto Tsunoda, Yuma Ishida, Mao Kunimitsu","doi":"10.15583/jpchrom.2023.021","DOIUrl":"https://doi.org/10.15583/jpchrom.2023.021","url":null,"abstract":"","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138604174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liquid Chromatography Based Multiplex Gene Expression Analysis Using Fluorous Separation Technique with Fluorescence Detection 基于荧光检测荧光分离技术的液相色谱多重基因表达分析
Chromatography Pub Date : 2023-10-20 DOI: 10.15583/jpchrom.2023.020
Kenichiro TODOROKI, Chiemi MIYAUCHI, Tatsunosuke TOMITA, Jun Zhe MIN, Koichi INOUE, Toshimasa TOYO'OKA
{"title":"Liquid Chromatography Based Multiplex Gene Expression Analysis Using Fluorous Separation Technique with Fluorescence Detection","authors":"Kenichiro TODOROKI, Chiemi MIYAUCHI, Tatsunosuke TOMITA, Jun Zhe MIN, Koichi INOUE, Toshimasa TOYO'OKA","doi":"10.15583/jpchrom.2023.020","DOIUrl":"https://doi.org/10.15583/jpchrom.2023.020","url":null,"abstract":"We have developed a comprehensive method for analyzing gene expression levels by combining the fluorous-fluorescence hybrid probes, oligonucleic acids modified with a perfluoroalkyl tag and fluorescent probes, with the fluorous liquid chromatography (LC) – fluorescence detection. When Taq polymerase chain reaction (PCR) was performed using this probe, fluorescent mononucleotides were released according to the amount of template gene targeted. Because each probe differs in the linker length of the fluorescent probe and the type of nucleobase, multiple gene expression levels can be analyzed by LC as the amount of fluorescent mononucleotides produced. The excess unreacted probe, which did not undergo the Taq PCR reaction, was selectively removed by adsorption in the fluorous LC column introduced as a pretreatment column. The method established in this study allows simultaneous LC quantification of three housekeeping genes. In addition, LC separation conditions for the simultaneous analysis of nine gene expression levels were established, and comprehensive detection of cytokine genes in lipopolysaccharide-stimulated leukocytes was successfully achieved.","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135513814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elution Behavior of Carbohydrates Using Core-Shell Ion-Exchange Resin St-70 with Different Degrees of Cross-Linking in the Porous Shell 多孔壳中不同交联度核壳离子交换树脂St-70对碳水化合物的洗脱行为
Chromatography Pub Date : 2023-10-20 DOI: 10.15583/jpchrom.2023.008
Shun-ichi MITOMO, Yutaka INOUE, Takashi TANIKAWA, Yukiko NEGISHI
{"title":"Elution Behavior of Carbohydrates Using Core-Shell Ion-Exchange Resin St-70 with Different Degrees of Cross-Linking in the Porous Shell","authors":"Shun-ichi MITOMO, Yutaka INOUE, Takashi TANIKAWA, Yukiko NEGISHI","doi":"10.15583/jpchrom.2023.008","DOIUrl":"https://doi.org/10.15583/jpchrom.2023.008","url":null,"abstract":"A novel core-shell ion-exchange resin (St-70) having a core-shell monomer weight ratio of 30:70 was prepared for use in highperformance liquid chromatography (HPLC), and the effect of the degree of cross-linking (10–55%) of the porous shell on the separation of carbohydrates was examined. A mixture of inositol, glucose, fructose, and sucrose was separated under alkaline conditions (100 and 150 mmol/L NaOH) at flow rates of 0.3–0.7 mL/min using St-70, which exhibited a degree of cross-linking of 10, 40, and 55%. The retention time of carbohydrates decreased with increasing of the degree of cross-linking in the porous shell. The theoretical plate number (N) of glucose, fructose, and sucrose decreased with decreasing the degree of cross-linking when 100 mmol/L NaOH eluent was used at flow rates of 0.3 and 0.5 mL/min.","PeriodicalId":10272,"journal":{"name":"Chromatography","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135513815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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