{"title":"Machine learning allied to green tech analysis: Pioneering approaches for remineralizers and silicate fertilizers chemical composition assessment","authors":"Renata Andrade, Júlia Gonçalves Aguiar, Magda Bergmann, Liliane Lavoura Bueno Sachs, Sérgio Henrique Godinho Silva, Alberto Vasconcellos Inda, Marco Aurélio Carbone Carneiro, Nilton Curi","doi":"10.1590/1413-7054202549011825","DOIUrl":"https://doi.org/10.1590/1413-7054202549011825","url":null,"abstract":"ABSTRACT Traditional methods for the chemical characterization of agrominerals remain costly and generate chemical waste, especially for large-scale applications. Portable X-ray fluorescence (pXRF) spectrometry has recently emerged as a tool for rapid material analysis. However, pXRF applications to remineralizers (REM), silicate fertilizers (SiF), and other agrominerals remain unexplored. This study aimed to: i) apply pXRF data to characterize agrominerals, and ii) assess six machine learning algorithms [projection pursuit regression (PPR), partial least squares (PLS), random forest (RF), support vector machine (SVM), extreme gradient boosting (XGB), and cubist regression (CR)] for agrominerals chemical composition assessment. 53 REM and SiF samples underwent traditional wet-chemical analysis (ICM 40B method) and triplicate pXRF scanning (60 s). The dataset was randomly separated into modeling (70%) and validation (30%) sub-datasets, and the machine learning algorithms were trained using k-fold cross-validation. Results indicated highly accurate predictions. Among the 49 predicted elements, 41 showed high accuracy (Al, Ba, Be, Bi, Ca, Ce, Co, Cr, Cs, Cu, Fe, Ga, Ge, Hf, In, K, La, Li, Lu, Mg, Mn, Mo, Na, Nb, Ni, P, Pb, Rb, S, Sc, Sr, Tb, Th, Ti, Tl, U, V, Y, Yb, Zn, and Zr) with R2 above 0.80, and RPD values ranging from 1.31 to 23.0. The presence of nutrients such as Ca, Mg, K, S, Fe, Mn, Zn, and P stood out. CR outperformed the other algorithms. The study demonstrates the potential of using pXRF combined with machine learning algorithms for accurate, rapid, cheap and environmentally friendly prediction of the chemical composition of agrominerals.","PeriodicalId":10191,"journal":{"name":"Ciência e Agrotecnologia","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro propagation and root anatomical features of Phragmipedium kovachii J.T. Atwood, Dalström & Ric.Fernández","authors":"Muthab Hussien, О. И. Молканова, Vladimir Koval","doi":"10.1590/1413-7054202549002125","DOIUrl":"https://doi.org/10.1590/1413-7054202549002125","url":null,"abstract":"ABSTRACT Phragmipedium kovachii is a renowned orchid for its striking flowers and ecological significance. This study aimed to develop an efficient protocol for its micropropagation and conservation. Shoots measuring 0.7-1.0 cm were used as explants and cultured on half-strength Murashige and Skoog (MS) medium with various combinations of 6-benzylaminopurine (6-BAP) and indole-3-acetic acid (IAA), with or without 100 mL L-¹ coconut water. The highest protocorm-like bodies (PLBs) formation rate (96.7%) and the maximum PLBs per explant (12.58 ± 0.33) were obtained on medium containing 2.0 mg L-¹ 6-BAP, 0.6 mg L-¹ IAA, and coconut water. During the regeneration phase, PLBs regenerated on media supplemented with either 0.5 mg L-¹ 6-BAP and coconut water, as well as those on media containing 0.5 mg L-¹ meta-Topolin (mT) and banana homogenate, produced optimal plantlets for subsequent rooting stages. Rooting was enhanced using 1.0 mg L-¹ IAA with coconut water or 0.5 mg L-¹ indole-3-butyric acid (IBA) with banana homogenate. Plantlets grown on coconut water-supplemented media developed longer roots with a pentarch stele, well-differentiated sclerenchyma tissue, and numerous metaxylem vessels, exhibiting the highest survival rate. In contrast, those cultured on banana homogenate produced thicker roots with a polyarch stele. Acclimatization was successfully achieved using substrates consisting of equal proportions of bark, perlite, and peat, as well as a mixture of soil, sand and perlite (2:1:1), ensuring high ex vitro survival rates (87 and 80%, respectively). This study provides an effective protocol for the ex situ conservation and large-scale production of P. kovachii plants.","PeriodicalId":10191,"journal":{"name":"Ciência e Agrotecnologia","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scielo.br/j/cagro/a/PxBCfB66x9j94cdpftphbPx/?lang=en&format=pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}