环境科学与生态学最新文献

{"title":"Effects of mefentrifluconazole on soil microorganisms, enzymes and earthworms in sandy soil and clay loam of peanut fields.","authors":"Mengke Wang, Qingchen Zhao, Chaofan Jiang, Te Zhao, Kaidi Cui, Lin Zhou, Leiming He","doi":"10.1016/j.ecoenv.2025.118684","DOIUrl":"10.1016/j.ecoenv.2025.118684","url":null,"abstract":"<p><p>Our previous study indicated that mefentrifluconazole had a high efficacy in the control of peanut southern blight. However, the soil ecological risks of mefentrifluconazole in peanut fields were largely unknown. In this study, mefentrifluconazole was not readily degraded in soil that 0.1 mg/kg of mefentrifluconazole only degraded by 32.00 % and 35.00 % in sandy soil and clay loam after 60 days. It was also poorly vertically transported in soil that the relative displacement (R_f) values for mefentrifluconazole in both types of soil were 0.25. Moreover, substantial mefentrifluconazole was accumulated at a soil depth of 3-6 cm in both types. Consistent with residue dynamics, mefentrifluconazole had a long-term effect on soil microorganisms and enzymes. After 60 days, soil actinomycetes and bacteria recovered to control levels whereas fungi remained obviously lower than the control. It took at least 45 days for soil enzymes (urease, phosphatase and sucrase) to return to control levels. However, mefentrifluconazole exhibited low toxicity to earthworms. Only 14 days of exposure to 100 mg/kg of mefentrifluconazole resulted in significant weight loss in earthworms. In addition, earthworms showed obvious avoidance behavior toward mefentrifluconazole under natural leaching conditions. Above results suggested that mefentrifluconazole had prolonged effects on soil microorganisms and enzymes but showed low toxicity to earthworms.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118684"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the pathways of heavy metal accumulation in rice: roles of roots, stems, and soil pH.","authors":"Yang Yu, Hao Zheng, Qi Chen, Juan Fei, Hong Sun, Zhen Ding","doi":"10.1016/j.ecoenv.2025.118664","DOIUrl":"10.1016/j.ecoenv.2025.118664","url":null,"abstract":"<p><p>Heavy metal contamination in agricultural soils is a global concern, yet the roles of roots, stems, and soil pH in metal uptake by rice remain underexplored. This study evaluates the ecological and health risks of soil and rice contamination and examines how metal levels in soil, roots, stems, and soil pH affect accumulation in rice grains. A total of 520 paired soil-grain samples and 40 comprehensive sets including soil, rice roots, stems, and grains were collected and analyzed. The results indicated that the ecological and health risks associated with heavy metals were generally low. However, accumulation in rice roots and stems may contribute to secondary soil contamination. Grain Hg and Cu concentrations showed positive, non-linear associations with their respective soil levels. Cd in grains was positively and non-linearly associated with root concentrations, while Cu showed a positive linear relationship with root levels and a negative linear relationship with stem levels, suggesting that stems may act as a barrier to Cu translocation. Cr in grains was positively and non-linearly associated with stem concentrations. Soil pH significantly influenced uptake: Cd absorption was enhanced under acidic conditions (pH ≤ 5.5) and inhibited under alkaline conditions (pH > 7.5); Hg uptake increased at pH > 6.5; and Cu and Cr uptake were higher at pH > 7.5. This study fills key knowledge gaps by clarifying the roles of roots and stems in metal translocation and offers insights into mitigating heavy metal accumulation in rice through soil pH management.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118664"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-driven optimization of arsenic phytoextraction using amendments.","authors":"Huading Shi, Yunxian Yan, Zhaoyang Han, Liang Wang, Guanghui Guo, Jun Yang","doi":"10.1016/j.ecoenv.2025.118705","DOIUrl":"10.1016/j.ecoenv.2025.118705","url":null,"abstract":"<p><p>Exogenous amendments are crucial for enhancing the remediation efficiency of arsenic-contaminated soils by Pteris vittata. However, their effectiveness is unstable due to various factors, and neglecting their economic costs hinder broader application. In this study, we analyzed 2299 data points from 121 published datasets and used machine learning to predict and optimize the performance of amendments to enhance the phytoextraction efficiency. Using a random forest model, we predicted changes in As accumulation in P. vittata in response to specific amendments, considering 18 parameters across four categories: changes in P. vittata, amendments, soil properties, and cultivation conditions. The model achieved an R² value of 0.846. Using %IncMSE to quantify parameter contribution, we found that the biomass of P. vittata had a greater influence than the As concentration. Additionally, amendment type, application time, cultivation duration, and soil-available As were key factors in enhancing As accumulation in P. vittata. Regarding economic cost, different amendments required an investment ranging from 0.57 to 3903.86 CNY to enhance 1 g of As accumulation in P. vittata. Among these, phosphate fertilizers had the lowest cost, whereas calcium acetate, ethylenediamine-N,N'-disuccinic acid, and glutathione did not have economic advantages as amendments. This study offers guidance on the development of amendments, providing an important reference for the practical application of phytoextraction in As-contaminated soils.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118705"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenol A in utero induced glutamate and D-serine metabolic dysregulation in the hippocampus of rats and primary cultured astrocytes.","authors":"Luxin Zhang, Xinran Li, Yichen Zhao, Panjie Wang, Mengwenhui Shi, Xiao Li, Xiucong Pei, Zhiwen Duan, Mingyue Ma, Haiyang Yu","doi":"10.1016/j.ecoenv.2025.118651","DOIUrl":"10.1016/j.ecoenv.2025.118651","url":null,"abstract":"<p><p>Bisphenol A (BPA) is a widely used synthetic compound that could cause neurobehavioral abnormalities in mammals. Previous studies have suggested that NMDAR may be a potential target of BPA-induced neurotoxicity. However, the impact of exposure to BPA on glutamate (Glu) and D-serine (D-ser) metabolism (key regulators of NMDAR activation) has not been clarified yet. Pregnant SD rats were exposed to 0, 0.05, 0.5, 5 and 50 mg/kg/day BPA via oral gavage from gestational day (GD) 5 to GD 19, and primary cultured astrocytes (AS) from neonatal rats were exposed to 5, 10 and 20 μmol/L BPA, respectively. Neurochemical experiments were conducted to investigate dysfunctions in Glu and D-ser metabolism both in the hippocampus of rats' offspring at different developmental stages and in AS. Results showed that BPA exposure in utero induced Glu accumulation and inhibited GS, GLS1, and GDH expression and activity at the gene, protein, and enzymatic levels in the hippocampus of rats' offspring during different developmental stages (GD 20, PND 21, and PND 56). However, BPA exposure in utero increased D-ser levels at GD 20 but decreased them from PND 21 onward. Additionally, BPA exposure in utero inhibited SR, asc-1, and ASCT2 expression, while promoting ASCT1 expression during these stages. Besides, BPA exposure in utero up-regulated DAAO expression at GD 20 but down-regulated it from PND 21 onward. Moreover, BPA exposure inhibited the expression and activity of GS (except for its expression), GLS1, and GDH, while suppressing SR and DAAO expression but increasing ASCT2 expression without altering ASCT21 expression in AS. These findings will provide new insights into the pathogenesis of BPA-induced neurotoxicity through Glu and D-ser metabolic pathways.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118651"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal anti-androgen activity in the brackish Baltic waters in relation to the gene expression profiles of the blue mussel Mytilus trossulus.","authors":"Paulina Goździk, Katarzyna Smolarz, Magda Caban, Anna Hallmann","doi":"10.1016/j.ecoenv.2025.118660","DOIUrl":"10.1016/j.ecoenv.2025.118660","url":null,"abstract":"<p><p>Endocrine disruptors (EDs) in aquatic environments pose significant risks to marine organisms, affecting critical physiological processes such as reproduction, metabolism, and stress responses. The Baltic Sea, with its high anthropogenic pressure and unique ecological conditions, is particularly vulnerable to EDs contamination. Our study aimed to evaluate the endocrine-disrupting, metabolism-impairing, and cytotoxic effects of water samples collected seasonally from a sewage treatment plant outlet in the Gulf of Gdańsk (Poland). Simultaneously, the gonadal transcriptome profiles of Mytilus trossulus were analyzed to investigate potential responses at the molecular level. Anti-androgenic activity of water samples, quantified using the XenoScreen YAS assay, was the highest in autumn (36.47 µM flutamide equivalents) and winter (23.57 μM), decreasing in spring (4.48 μM) and summer (0.54 μM). No estrogenic or anti-estrogenic activity was detected in the collected water samples tested with the YES assay and using the cell line BT-474. RNA-sequencing of mussel gonads enabled the identification of seasonal transcriptomic changes linked to gametogenesis, steroid and lipid metabolism, and detoxification processes. Seasonal fluctuations in anti-androgenic activity were observed in water samples, accompanied by distinct shifts in gene expression patterns in mussels. While no acute physiological disturbances were detected, the ongoing anthropogenic impact on the Baltic Sea underscores the need for continued monitoring. The findings highlight the potential for EDs-induced disruptions in marine organisms, emphasizing the importance of mitigating human-caused environmental stressors to protect aquatic biodiversity.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118660"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probabilistic trophic magnification analysis for assessing the bioaccumulation potential of organophosphate flame retardants in freshwater ecosystems of South Korea.","authors":"Dokyun Kim, Eun-Ji Won, Ha-Eun Cho, Hyeong-Wook Jo, Ki-Young Choi, Chang-Joon Kim, Kyung-Hoon Shin","doi":"10.1016/j.ecoenv.2025.118657","DOIUrl":"10.1016/j.ecoenv.2025.118657","url":null,"abstract":"<p><p>Despite considerable efforts in recent years to quantify the trophic magnification factor (TMF) of organophosphate flame retardants (OPFRs), definitive conclusions regarding their biomagnification potential have not yet been reached. This study hypothesizes that sampling uncertainty may affect TMF calculations. To overcome the limitations of traditional regression-based methods (TMF_R), we applied a Monte Carlo simulation-based probabilistic method (TMF_M), which provides more reliable estimates of OPFR bioaccumulation in freshwater ecosystems. The concentrations of tributyl phosphate (TNBP), tris(2-chloroethyl) phosphate (TCEP), and tris(2-chloroisopropyl) phosphate (TCIPP) were analyzed in relation to the amino acid stable isotope-based trophic position in fish from four major rivers in Korea. The TMF_M values were 1.2 ± 0.3, 1.4 ± 0.9, and 2.0 ± 1.9 for TNBP, TCEP, and TCIPP, respectively, with TCIPP exhibiting a relatively higher biomagnification potential, consistent with previous findings. In contrast, the TMF_R produced extreme values (e.g., 17.8 for TCIPP), whereas TMF_M moderated by approximately 50 %, reducing spatial and temporal variability. A meta-analysis of literature data further supported the TMF_M estimates, revealing that 80 % of TMFs ranged between 0 and 2, while higher values were observed for top predators and specific ecosystems. These results advance the understanding of OPFR bioaccumulation by improving TMF estimation accuracy and highlight the need for ongoing monitoring and mechanistic studies. The probabilistic approach presented here offers a valuable tool for environmental risk assessment and supports informed chemical management.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118657"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal air temperature exposure and risk of small vulnerable newborn: Identifying critical windows.","authors":"Mingzhao Huang, Jian Fang, Yaqi Zhang, Luli Xu, Xiya Qin, Kai Chen, Zhiguo Xia, Jun Li, Lianting Hu, Xiaofeng Mu, Xiaoxuan Fan, Youjie Wang, Aifen Zhou, Chao Xiong","doi":"10.1016/j.ecoenv.2025.118727","DOIUrl":"10.1016/j.ecoenv.2025.118727","url":null,"abstract":"<p><strong>Background: </strong>Small vulnerable newborn (SVN), defined as preterm birth (PTB), small for gestational age (SGA), or low birth weight (LBW), remains a global health challenge. While prenatal temperature exposure may influence pregnancy outcomes, existing trimester-level analyses lack temporal precision to detect sensitive gestational windows. This population-based study aims to explore the relationship between temperature and SVN over time and identify critical exposure periods with weekly resolution.</p><p><strong>Methods: </strong>Using a retrospective cohort of 1,129,572 singleton births (2011-2022) in Wuhan, China, we linked daily ambient temperature data at 1-km² resolution to maternal residential coordinates. Extended distributed lag non-linear models (Extended-DLNMs) combined with logistic regression examined associations between weekly temperature exposure and SVN outcomes.</p><p><strong>Results: </strong>Of the births, 14.5 % were classified as SVN (7.49 % PTB, 4.66 % LBW, 7.58 % SGA). Exposure to heat (28.8°C v.s. 18.6°C) and cold (5.3°C v.s. 18.6°C) during pregnancy was associated with increased SVN risks, primarily driven by PTB and LBW. Heat exposure during weeks 10-23 and 34-37 increased PTB risk, peaking at week 37 (OR: 1.11, 95 % CI: 1.09-1.13). Cold exposure during weeks 1-13 and 22-33 increased PTB risk, most notably at week 28 (OR: 1.09, 95 % CI: 1.07-1.10). For LBW, heat exposure during weeks 9-24 and 37-42 was associated with increased risks, strongest at week 42 (OR: 1.19, 95 % CI: 1.16-1.21). Cold exposure during weeks 1-6 and 23-34 correlated with elevated LBW risks, particularly at week 29 (OR: 1.08, 95 % CI: 1.05-1.11).</p><p><strong>Conclusions: </strong>While the overall exposure-response relationship exhibits a U-shaped pattern throughout gestation, our findings reveal substantial heterogeneity in temperature sensitivity across developmental windows. Some gestational stages are more susceptible to heat, whereas others exhibit increased vulnerability to cold. Further mechanistic research is warranted to elucidate the placental and fetal adaptation pathways under temperature extremes.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118727"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel extraction method of prymnesins from Prymnesium parvum whole culture samples and re-evaluation of existing protocols.","authors":"Magdalena Pöchhacker, Alexander Conrad, Doris Marko, Elisabeth Varga","doi":"10.1016/j.ecoenv.2025.118745","DOIUrl":"10.1016/j.ecoenv.2025.118745","url":null,"abstract":"<p><p>Harmful algal blooms caused by Prymnesium parvum can result in massive fish kills. The ichthyotoxins suspected to be responsible, known as prymnesins (PRMs), produced by P. parvum are classified into A-, B-, and C-types. In 2022, a severe HAB event within the Odra/Oder River impacted the environment via these toxins. The detection of PRMs is still a challenge due to a lack of analytical standards and stability issues along with losses during conventional extraction methods. In this study, PRM recovery was assessed for the three most common extraction methods for microalgae, solid-phase extraction (SPE), solid-liquid extraction (SLE), and liquid-liquid extraction (LLE). Furthermore, the impacts from sonication, freezing, and different solvents upon PRM stability were evaluated. Within methanol (MeOH), PRMs remained stable but within aqueous solvents, PRMs degraded rapidly. A novel 50 % MeOH SPE approach was developed, which outperformed LLE and SLE. This method offers advantages for salty whole culture samples through desalination and reduces PRM losses by the omission of reconstitution and evaporation steps. Ethanol as greener alternative to MeOH resulted in lower PRM yields compared to MeOH but showed similar recovery to LLE and still had advantages, besides the ecological ones, like faster and easier handling. The method's robustness was confirmed across all algal growth stages and for all PRM classes, including 6, 4 and 3 analogs for A-, B-, and C-types, respectively. The optimized SPE approach provides a faster, cleaner and more reliable extraction method for PRMs and improves analytical workflows for both natural and laboratory samples.</p>","PeriodicalId":303,"journal":{"name":"Ecotoxicology and Environmental Safety","volume":"302 ","pages":"118745"},"PeriodicalIF":6.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent molecular composition of dissolved organic matter in biochar-amended manure composts.","authors":"Nennen Zhu, Ao Cheng, Xufang Yu, Xiaolong Liu, Ting Li, Xiujuan Zhou, Wenchao Ji, Xingjun Fan","doi":"10.1016/j.jenvman.2025.126770","DOIUrl":"10.1016/j.jenvman.2025.126770","url":null,"abstract":"<p><p>Biochar (BC) amendment is a promising strategy for enhancing compost humification, yet its dose-dependent effects on the molecular-level characteristics of dissolved organic matter (DOM) remain unclear. This study systematically investigates the molecular composition of DOM in compost amended with 0 % (CK), 1 % (T1), 2 % (T2), and 5 % (T5) BC. Elemental analysis revealed a progressive increase in N content from 3.46 % (CK) to 4.56 % (T5), indicating enhanced N retention. Spectroscopic analysis indicated an increase in humification and enrichment of O-containing groups, particularly C-O, related to carbohydrate-like substances. High-performance size exclusion chromatography showed that BC additions increased the abundance of medium molecular weight (0.5-8.0 kDa) while reducing high molecular weight (>8 kDa) fractions, resulting in a slight decrease in weight-average molecular weight (Mw) from 23.76 kDa to 20.32-22.35 kDa. High-resolution mass spectrometry revealed that BC amendment, particularly at 5 %, led to lower oxidation, reduced aromaticity, and a higher proportion of saturated, aliphatic CHO compounds. Molecular-level analysis showed substantial degradation of lignins/CRAM compounds (39 %-56 %) and CHON species (33 %-41 %), alongside the formation of carbohydrates (21-33 %) and CHO species (53-79 %). Overall, this study highlights that BC dosage significantly alters DOM molecular composition, providing mechanistic insights into humification enhancement. The findings offer valuable implications for improving compost quality and suggest that even low BC dosages could serve as a cost-effective and scalable strategy in composting practices.</p>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"392 ","pages":"126770"},"PeriodicalIF":8.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entrepreneurship and ecological sustainability in EU-5: A panel quantile approach.","authors":"Muhammet Daştan, Ismail Demirdag, Abdullah Emre Caglar","doi":"10.1016/j.jenvman.2025.126802","DOIUrl":"10.1016/j.jenvman.2025.126802","url":null,"abstract":"<p><p>The 2035 and 2050 climate targets have accelerated the European Union's (EU) commitment to decarbonization policies. While public sector initiatives remain crucial, the private sector, particularly entrepreneurial activities, plays an equally important role in shaping environmental well-being. Given their growing significance within the EU economies, these activities must be considered fundamental components in designing effective environmental policies. In this context, the present study employs a method of moments quantile approach to examine the impact of entrepreneurship on environmental sustainability across five EU countries (France, Germany, Italy, Spain, and the Netherlands) from 2001 to 2022, framed within the load capacity curve hypothesis. Additionally, the study evaluates the combined effects of green energy consumption and urbanization, recognizing their interconnected influence on ecological sustainability. Empirical results indicate that the load capacity curve hypothesis does not hold for these nations. Moreover, green energy consumption and urbanization significantly enhance environmental sustainability, while entrepreneurship is linked to a decline in environmental quality. Given the environmental challenges associated with entrepreneurship in the EU, it is imperative to develop and enforce policies that promote environmentally sustainable business practices. One possible measure could be the mandatory adoption of environmentally friendly certifications, such as ISO standards, for new enterprises. This study concludes with a discussion of policy implications aimed at fostering a sustainable environment through green entrepreneurship and supportive regulatory frameworks.</p>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"392 ","pages":"126802"},"PeriodicalIF":8.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}