Fly最新文献_第6页

Flying remote. 远程飞行。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1884033

Howy Jacobs

引用次数: 0

In vivo assay and modelling of protein and mitochondrial turnover during aging. 衰老过程中蛋白质和线粒体周转的活体检测和建模。

IF 2.4 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1911286

Hans S Bell, John Tower

{"title":"In vivo assay and modelling of protein and mitochondrial turnover during aging.","authors":"Hans S Bell, John Tower","doi":"10.1080/19336934.2021.1911286","DOIUrl":"10.1080/19336934.2021.1911286","url":null,"abstract":"To maintain homoeostasis, cells must degrade damaged or misfolded proteins and synthesize functional replacements. Maintaining a balance between these processes, known as protein turnover, is necessary for stress response and cellular adaptation to a changing environment. Damaged mitochondria must also be removed and replaced. Changes in protein and mitochondrial turnover are associated with aging and neurodegenerative disease, making it important to understand how these processes occur and are regulated in cells. To achieve this, reliable assays of turnover must be developed. Several methods exist, including pulse-labelling with radioactive or stable isotopes and strategies making use of fluorescent proteins, each with their own advantages and limitations. Both cell culture and live animals have been used for these studies, in systems ranging from yeast to mammals. In vivo assays are especially useful for connecting turnover to aging and disease. With its short life cycle, suitability for fluorescent imaging, and availability of genetic tools, Drosophila melanogaster is particularly well suited for this kind of analysis.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"15 1","pages":"60-72"},"PeriodicalIF":2.4,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143256/pdf/KFLY_15_1911286.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38993629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Excitotoxicity and Regulation of a Constitutively Active TRP Ca²⁺ Channel in Drosophila. 探索果蝇组成活性TRP Ca2+通道的兴奋毒性和调控。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 Epub Date: 2020-12-01 DOI: 10.1080/19336934.2020.1851586

Bih-Hwa Shieh, Lucinda Nuzum, Inga Kristaponyte

{"title":"Exploring Excitotoxicity and Regulation of a Constitutively Active TRP Ca2+ Channel in Drosophila.","authors":"Bih-Hwa Shieh, Lucinda Nuzum, Inga Kristaponyte","doi":"10.1080/19336934.2020.1851586","DOIUrl":"https://doi.org/10.1080/19336934.2020.1851586","url":null,"abstract":"Unregulated Ca2+ influx affects intracellular Ca2+ homoeostasis, which may lead to neuronal death. In Drosophila, following the activation of rhodopsin the TRP Ca2+ channel is open to mediate the light-dependent depolarization. A constitutively active TRP channel triggers the degeneration of TrpP365 /+ photoreceptors. To explore retinal degeneration, we employed a multidisciplinary approach including live imaging using GFP tagged actin and arrestin 2. Importantly, we demonstrate that the major rhodopsin (Rh1) was greatly reduced before the onset of rhabdomere degeneration; a great reduction of Rh1 affects the maintenance of rhabdomere leading to degeneration of photoreceptors. TrpP365 /+ also led to the up-regulation of CaMKII, which is beneficial as suppression of CaMKII accelerated retinal degeneration. We explored the regulation of TRP by investigating the genetic interaction between TrpP365 /+ and mutants affecting the turnover of diacylglycerol (DAG). We show a loss of phospholipase C in norpAP24 exhibited a great reduction of the DAG content delayed degeneration of TrpP365 /+ photoreceptors. In contrast, knockdown or mutations in DAG lipase (InaE) that is accompanied by slightly reduced levels of most DAG but an increased level of DAG 34:1, exacerbated retinal degeneration of TrpP365 /+. Together, our findings support the notion that DAG plays a role in regulating TRP. Interestingly, DAG lipase is likely required during photoreceptor development as TrpP365 /+; inaEN125 double mutants contained severely degenerated rhabdomeres.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"15 1","pages":"8-27"},"PeriodicalIF":1.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2020.1851586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38611004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Characterizing a gene expression toolkit for eye- and photoreceptor-specific expression in Drosophila. 鉴定果蝇眼部和感光器特异性基因表达工具包。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1915683

Spencer E Escobedo, Aashka Shah, Alyssa N Easton, Hana Hall, Vikki M Weake

{"title":"Characterizing a gene expression toolkit for eye- and photoreceptor-specific expression in Drosophila.","authors":"Spencer E Escobedo, Aashka Shah, Alyssa N Easton, Hana Hall, Vikki M Weake","doi":"10.1080/19336934.2021.1915683","DOIUrl":"10.1080/19336934.2021.1915683","url":null,"abstract":"Binary expression systems are a powerful tool for tissue- and cell-specific research. Many of the currently available Drosophila eye-specific drivers have not been systematically characterized for their expression level and cell-type specificity in the adult eye or during development. Here, we used a luciferase reporter to measure expression levels of different drivers in the adult Drosophila eye, and characterized the cell type-specificity of each driver using a fluorescent reporter in live 10-day-old adult males. We also further characterized the expression pattern of these drivers in various developmental stages. We compared several Gal4 drivers from the Bloomington Drosophila Stock Center (BDSC) including GMR-Gal4, longGMR-Gal4 and Rh1-Gal4 with newly developed Gal4 and QF2 drivers that are specific to different cell types in the adult eye. In addition, we generated drug-inducible Rh1-GSGal4 lines and compared their induced expression with an available GMR-GSGal4 line. Although both lines had significant induction of gene expression measured by luciferase activity, Rh1-GSGal4 was expressed at levels below the detection of the fluorescent reporter by confocal microscopy, while GMR-GSGal4 showed substantial reporter expression in the absence of drug by microscopy. Overall, our study systematically characterizes and compares a large toolkit of eye- and photoreceptor-specific drivers, while also uncovering some of the limitations of currently available expression systems in the adult eye.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"15 1","pages":"73-88"},"PeriodicalIF":1.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2021.1915683","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38908488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Drosophila melanogaster: a fruitful model for oncohistones. 黑腹果蝇:嗜瘤蛋白丰富的模型。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2020.1863124

Amel Chaouch, Paul Lasko

引用次数: 0

Drosophila as a useful model for understanding the evolutionary physiology of obesity resistance and metabolic thrift. 果蝇是理解肥胖抵抗和代谢节俭的进化生理学的有用模型。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1896960

Lindsey J Gray, Marla B Sokolowski, Stephen J Simpson

{"title":"Drosophila as a useful model for understanding the evolutionary physiology of obesity resistance and metabolic thrift.","authors":"Lindsey J Gray, Marla B Sokolowski, Stephen J Simpson","doi":"10.1080/19336934.2021.1896960","DOIUrl":"https://doi.org/10.1080/19336934.2021.1896960","url":null,"abstract":"Evolved metabolic thriftiness in humans is a proposed contributor to the obesity epidemic. Insect models have been shown to evolve both 'metabolic thrift' in response to rearing on high-protein diets that promote leanness, and 'obesity resistance' when reared on fattening high-carbohydrate, low-protein foods. Despite the hypothesis that human obesity is caused by evolved metabolic thrift, genetic contributions to this physiological trait remain elusive. Here we conducted a pilot study to determine whether thrift and obesity resistance can arise under laboratory based 'quasi-natural selection' in the genetic model organism Drosophila melanogaster. We found that both these traits can evolve within 16 generations. Contrary to predictions from the 'thrifty genotype/phenotype' hypothesis, we found that when animals from a metabolic thrift inducing high-protein environment are mismatched to fattening high-carbohydrate foods, they did not become 'obese'. Rather, they accumulate less triglyceride than control animals, not more. We speculate that this may arise through as yet un-quantified parental effects - potentially epigenetic. This study establishes that D. melanogaster could be a useful model for elucidating the role of the trans- and inter-generational effects of diet on the genetics of metabolic traits in higher animals.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"15 1","pages":"47-59"},"PeriodicalIF":1.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2021.1896960","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25475814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup. PseudoBase：假褐腹果蝇亚群的基因组可视化和探索资源。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2020.1864201

Katharine L Korunes, Russell B Myers, Ryan Hardy, Mohamed A F Noor

{"title":"PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup.","authors":"Katharine L Korunes, Russell B Myers, Ryan Hardy, Mohamed A F Noor","doi":"10.1080/19336934.2020.1864201","DOIUrl":"10.1080/19336934.2020.1864201","url":null,"abstract":"Drosophila pseudoobscura is a classic model system for the study of evolutionary genetics and genomics. Given this long-standing interest, many genome sequences have accumulated for D. pseudoobscura and closely related species D. persimilis, D. miranda, and D. lowei. To facilitate the exploration of genetic variation within species and comparative genomics across species, we present PseudoBase, a database that couples extensive publicly available genomic data with simple visualization and query tools via an intuitive graphical interface, amenable for use in both research and educational settings. All genetic variation (SNPs and indels) within the database is derived from the same workflow, so variants are easily comparable across data sets. Features include an embedded JBrowse interface, ability to pull out alignments of individual genes/regions, and batch access for gene lists. Here, we introduce PseudoBase, and we demonstrate how this resource facilitates use of extensive genomic data from flies of the Drosophila pseudoobscura subgroup.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"15 1","pages":"38-44"},"PeriodicalIF":1.2,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808432/pdf/KFLY_15_1864201.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38714228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm viability varies with buffer and genotype in Drosophila melanogaster. 黑腹果蝇精子活力随缓冲液和基因型的不同而不同。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 Epub Date: 2020-12-08 DOI: 10.1080/19336934.2020.1837592

Ruijian Guo, Anna-Lena Henke, Klaus Reinhardt

引用次数: 1

Dawning of the open era. 开放时代的曙光。

IF 1.2 4区生物学

Fly Pub Date : 2021-12-01 DOI: 10.1080/19336934.2021.1943285

Howy Jacobs

引用次数: 0

Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions. 果蝇GFAT1和GFAT2酶编码专性发育功能。