IF 1.2 4区生物学

Fly Pub Date : 2018-01-02 Epub Date: 2017-12-12 DOI: 10.1080/19336934.2017.1402994

Trevor L Davis, Ilaria Rebay

{"title":"Pleiotropy in Drosophila organogenesis: Mechanistic insights from Combgap and the retinal determination gene network.","authors":"Trevor L Davis, Ilaria Rebay","doi":"10.1080/19336934.2017.1402994","DOIUrl":"https://doi.org/10.1080/19336934.2017.1402994","url":null,"abstract":"Master regulatory transcription factors cooperate in networks to shepherd cells through organogenesis. In the Drosophila eye, a collection of master control proteins known as the retinal determination gene network (RDGN) switches the direction and targets of its output to choreograph developmental transitions, but the molecular partners that enable such regulatory flexibility are not known. We recently showed that two RDGN members, Eyes absent (Eya) and Sine oculis (So), promote exit from the terminal cell cycle known as the second mitotic wave (SMW) to permit differentiation. A search for co-factors identified the ubiquitously expressed Combgap (Cg) as a novel transcriptional partner that impedes cell cycle exit and interferes with Eya-So activity specifically in this context. Here, we argue that Cg acts as a flexible transcriptional platform that contributes to numerous gene expression outcomes by a variety of mechanisms. For example, Cg provides repressive activities that dampen Eya-So output, but not by recruiting Polycomb chromatin-remodeling complexes as it does in other contexts. We propose that master regulators depend on both specifically expressed co-factors that assemble the combinatorial code and broadly expressed partners like Cg that recruit the diverse molecular activities needed to appropriately regulate their target enhancers.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 1","pages":"62-70"},"PeriodicalIF":1.2,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1402994","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35595263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

MTV sings jubilation for telomere biology in Drosophila. MTV为《果蝇》的端粒生物学欢呼雀跃。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-02 Epub Date: 2017-06-02 DOI: 10.1080/19336934.2017.1325979

Lin Cheng, Ming Cui, Yikang S Rong

{"title":"MTV sings jubilation for telomere biology in Drosophila.","authors":"Lin Cheng, Ming Cui, Yikang S Rong","doi":"10.1080/19336934.2017.1325979","DOIUrl":"10.1080/19336934.2017.1325979","url":null,"abstract":"Telomere protects the ends of linear chromosomes. Telomere dysfunction fuels genome instability that can lead to diseases such as cancer. For over 30 years, Drosophila has fascinated the field as the only major model organism that does not rely on the conserved telomerase enzyme for end protection. Instead of short DNA repeats at chromosome ends, Drosophila has domesticated retrotransposons. In addition, telomere protection can be entirely sequence-independent under normal laboratory conditions, again dissimilar to what has been established for telomerase-maintained systems. Despite these major differences, recent studies from us and others have revealed remarkable similarities between the 2 systems. In particular, with the identification of the MTV complex as an ssDNA binding complex essential for telomere integrity in Drosophila (Zhang et al. 2016 Plos Genetics), we have now established several universal principles that are intrinsic to chromosome extremities but independent of the underlying DNA sequences or the telomerase enzyme. Telomere studies in Drosophila will continue to yield fundamental insights that are instrumental to the understanding of the evolution of telomere and telomeric functions.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 1","pages":"41-45"},"PeriodicalIF":1.2,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1325979","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34968291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Compromising asymmetric stem cell division in Drosophila central brain: Revisiting the connections with tumorigenesis. 果蝇中脑的不对称干细胞分裂:与肿瘤发生的关系。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-02 Epub Date: 2018-01-08 DOI: 10.1080/19336934.2017.1416277

Ana Carmena

引用次数: 6

Deletion of the nicotinic acetylcholine receptor subunit gene Dα1 confers insecticide resistance, but at what cost? 烟碱乙酰胆碱受体亚基基因Dα1的缺失赋予了杀虫剂抗性，但代价是什么?

IF 1.2 4区生物学

Fly Pub Date : 2018-01-02 Epub Date: 2017-11-22 DOI: 10.1080/19336934.2017.1396399

Jason Somers, Hang Ngoc Bao Luong, Philip Batterham, Trent Perry

{"title":"Deletion of the nicotinic acetylcholine receptor subunit gene Dα1 confers insecticide resistance, but at what cost?","authors":"Jason Somers, Hang Ngoc Bao Luong, Philip Batterham, Trent Perry","doi":"10.1080/19336934.2017.1396399","DOIUrl":"https://doi.org/10.1080/19336934.2017.1396399","url":null,"abstract":"Nicotinic acetylcholine receptors (nAChRs) have vital functions in processes of neurotransmission that underpin key behaviors. These pentameric ligand-gated ion channels have been used as targets for insecticides that constitutively activate them, causing the death of insect pests. In examining a knockout of the Dα1 nAChR subunit gene, our study linked this one subunit with multiple traits. We were able to confirm previous work that had identified Dα1 as a target of the neonicotinoid class of insecticides. Further, we uncovered roles for the gene in influencing mating behavior and patterns of sleep. The knockout mutant was also observed to have a significant reduction in longevity. This study highlighted the severe fitness costs that appear to be associated with the loss of function of this gene in natural populations in the absence of insecticides targeting the Dα1 subunit. Such a fitness cost could explain why target site resistances to neonicotinoids in pest insect populations have been associated specific amino acid replacement mutations in nAChR subunits, rather than loss of function. That mutant phenotypes were observed for the two behaviors examined indicates that the functions of Dα1, and other nAChR subunits, need to be explored more broadly. It also remains to be established whether these phenotypes were due to loss of the Dα1 receptor and/or to compensatory changes in the expression levels of other nAChR subunits.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 1","pages":"46-54"},"PeriodicalIF":1.2,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1396399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35216322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Identifying the minimum number of microsatellite loci needed to assess population genetic structure: A case study in fly culturing. 确定评估种群遗传结构所需的最小微卫星位点数量:以蝇类养殖为例。

IF 2.4 4区生物学

Fly Pub Date : 2018-01-02 Epub Date: 2017-12-01 DOI: 10.1080/19336934.2017.1396400

Wolfgang Arthofer, Carina Heussler, Patrick Krapf, Birgit C Schlick-Steiner, Florian M Steiner

{"title":"Identifying the minimum number of microsatellite loci needed to assess population genetic structure: A case study in fly culturing.","authors":"Wolfgang Arthofer, Carina Heussler, Patrick Krapf, Birgit C Schlick-Steiner, Florian M Steiner","doi":"10.1080/19336934.2017.1396400","DOIUrl":"10.1080/19336934.2017.1396400","url":null,"abstract":"Small, isolated populations are constantly threatened by loss of genetic diversity due to drift. Such situations are found, for instance, in laboratory culturing. In guarding against diversity loss, monitoring of potential changes in population structure is paramount; this monitoring is most often achieved using microsatellite markers, which can be costly in terms of time and money when many loci are scored in large numbers of individuals. Here, we present a case study reducing the number of microsatellites to the minimum necessary to correctly detect the population structure of two Drosophila nigrosparsa populations. The number of loci was gradually reduced from 11 to 1, using the Allelic Richness (AR) and Private Allelic Richness (PAR) as criteria for locus removal. The effect of each reduction step was evaluated by the number of genetic clusters detectable from the data and by the allocation of individuals to the clusters; in the latter, excluding ambiguous individuals was tested to reduce the rate of incorrect assignments. We demonstrate that more than 95% of the individuals can still be correctly assigned when using eight loci and that the major population structure is still visible when using two highly polymorphic loci. The differences between sorting the loci by AR and PAR were negligible. The method presented here will most efficiently reduce genotyping costs when small sets of loci (\"core sets\") for long-time use in large-scale population screenings are compiled.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 1","pages":"13-22"},"PeriodicalIF":2.4,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35631723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strong responses of Drosophila melanogaster microbiota to developmental temperature. 黑腹果蝇微生物群对发育温度的强烈反应。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-02 Epub Date: 2017-12-07 DOI: 10.1080/19336934.2017.1394558

Neda N Moghadam, Pia Mai Thorshauge, Torsten N Kristensen, Nadieh de Jonge, Simon Bahrndorff, Henrik Kjeldal, Jeppe Lund Nielsen

{"title":"Strong responses of Drosophila melanogaster microbiota to developmental temperature.","authors":"Neda N Moghadam, Pia Mai Thorshauge, Torsten N Kristensen, Nadieh de Jonge, Simon Bahrndorff, Henrik Kjeldal, Jeppe Lund Nielsen","doi":"10.1080/19336934.2017.1394558","DOIUrl":"https://doi.org/10.1080/19336934.2017.1394558","url":null,"abstract":"Physiological responses to changes in environmental conditions such as temperature may partly arise from the resident microbial community that integrates a wide range of bio-physiological aspects of the host. In the present study, we assessed the effect of developmental temperature on the thermal tolerance and microbial community of Drosophila melanogaster. We also developed a bacterial transplantation protocol in order to examine the possibility of reshaping the host bacterial composition and assessed its influence on the thermotolerance phenotype. We found that the temperature during development affected thermal tolerance and the microbial composition of male D. melanogaster. Flies that developed at low temperature (13°C) were the most cold resistant and showed the highest abundance of Wolbachia, while flies that developed at high temperature (31°C) were the most heat tolerant and had the highest abundance of Acetobacter. In addition, feeding newly eclosed flies with bacterial suspensions from intestines of flies developed at low temperatures changed the heat tolerance of recipient flies. However, we were not able to link this directly to a change in the host bacterial composition.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 1","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2018-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2017.1394558","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35568217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

Localization and translation control of slam in Drosophila cellularization. 果蝇细胞化过程中slam的定位与翻译控制。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-09-18 DOI: 10.1080/19336934.2018.1520574

Shuling Yan, Jörg Großhans

{"title":"Localization and translation control of slam in Drosophila cellularization.","authors":"Shuling Yan, Jörg Großhans","doi":"10.1080/19336934.2018.1520574","DOIUrl":"https://doi.org/10.1080/19336934.2018.1520574","url":null,"abstract":"In this extra view, we comment on our recent work concerning the mRNA localization of the gene slow as molasses (slam). slam is a gene essential for the polarized invagination of the plasma membrane and separation of basal and lateral cortical domains during cellularization as well as for germ cell migration in later embryogenesis. We have demonstrated an intimate relationship between slam RNA and its encoded protein. Slam RNA co-localizes and forms a complex with its encoded protein. Slam mRNA localization not only is required for reaching full levels of functional Slam protein but also depends on Slam protein. The translation of slam mRNA is subject to tight spatio-temporal regulation leading to a rapid accumulation of Slam protein and zygotic slam RNA at the furrow canal. In this extra view, we first discuss the mechanism controlling localization and translation of slam RNA. In addition, we document in detail the maternal and zygotic expression of slam RNA and protein and provide data for a function in membrane stabilization. Furthermore, we mapped the region of Slam protein mediating cortical localization in cultured cells.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 3-4","pages":"191-198"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1520574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36485995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway. bantam microRNA是果蝇失肢通路的负调控因子。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-08-19 DOI: 10.1080/19336934.2018.1499370

Nanci S Kane, Mehul Vora, Richard W Padgett, Ying Li

引用次数: 6

Combining morphology and molecular data to improve Drosophila paulistorum (Diptera, Drosophilidae) taxonomic status. 结合形态学和分子数据来提高保氏果蝇（Diptera，Drosophilidae）的分类地位。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-02-07 DOI: 10.1080/19336934.2018.1429859

Rebeca Zanini, Mário Josias Müller, Gilberto Cavalheiro Vieira, Victor Hugo Valiati, Maríndia Deprá, Vera Lúcia da Silva Valente

{"title":"Combining morphology and molecular data to improve Drosophila paulistorum (Diptera, Drosophilidae) taxonomic status.","authors":"Rebeca Zanini, Mário Josias Müller, Gilberto Cavalheiro Vieira, Victor Hugo Valiati, Maríndia Deprá, Vera Lúcia da Silva Valente","doi":"10.1080/19336934.2018.1429859","DOIUrl":"10.1080/19336934.2018.1429859","url":null,"abstract":"The willistoni species subgroup has been the subject of several studies since the latter half of the past century and is considered a Neotropical model for evolutionary studies, given the many levels of reproductive isolation and different evolutionary stages occurring within them. Here we present for the first time a phylogenetic reconstruction combining morphological characters and molecular data obtained from 8 gene fragments (COI, COII, Cytb, Adh, Ddc, Hb, kl-3 and per). Some relationships were incongruent when comparing morphological and molecular data. Also, morphological data presented some unresolved polytomies, which could reflect the very recent divergence of the subgroup. The total evidence phylogenetic reconstruction presented well-supported relationships and summarized the results of all analyses. The diversification of the willistoni subgroup began about 7.3 Ma with the split of D. insularis while D.paulistorum complex has a much more recent diversification history, which began about 2.1 Ma and apparently has not completed the speciation process, since the average time to sister species separation is one million years, and some entities of the D. paulistorum complex diverge between 0.3 and 1 Ma. Based on the obtained data, we propose the categorization of the former \"semispecies\" of D. paulistorum as a subspecies and describe the subspecies D. paulistorum amazonian, D. paulistorum andeanbrazilian, D. paulistorum centroamerican, D. paulistorum interior, D. paulistorum orinocan and D. paulistorum transitional.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 2","pages":"81-94"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1429859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35754730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Epithelial tumors: Growing from within. 上皮性肿瘤:从内部生长。

IF 1.2 4区生物学

Fly Pub Date : 2018-01-01 Epub Date: 2018-03-01 DOI: 10.1080/19336934.2018.1441652

Mariana Muzzopappa, Marco Milán

{"title":"Epithelial tumors: Growing from within.","authors":"Mariana Muzzopappa, Marco Milán","doi":"10.1080/19336934.2018.1441652","DOIUrl":"https://doi.org/10.1080/19336934.2018.1441652","url":null,"abstract":"The growth of epithelial tumors is often governed by cell interactions with the surrounding stroma. Drosophila has been instrumental in identifying the relevant molecular elements mediating these interactions. Of note is the role of the TNF ligand Eiger, released from recruited blood cells, in activating the JNK tumor-promoting pathway in epithelial tumors. JNK drives the transcriptional induction of mitogenic molecules, matrix metalloproteases and systemic signals that lead to tumor growth, tissue invasiveness and malignancy. Here we review our findings on a tumor-intrinsic, Eiger- and stroma-independent mechanism that contributes to the unlimited growth potential of tumors caused either by chromosomal instability or impaired cell polarity. This newly identified mechanism, which was revealed in an experimental condition in which contacts between tumor cells and wild-type epithelial cells were minimized, relies on interactions between functionally distinct tumor cell populations that activate JNK in a cell-autonomous manner. We discuss the impact of cell interaction-based feedback amplification loops on the unlimited growth potential of epithelial tumors. These findings are expected to contribute to the identification of the relevant cell populations and molecular mechanisms to be targeted in drug therapy.","PeriodicalId":12128,"journal":{"name":"Fly","volume":"12 2","pages":"127-132"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19336934.2018.1441652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35837647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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