Cdc25Twine在果蝇母体向合子转化过程中降解的结构-功能分析

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fly Pub Date : 2022-02-28 DOI:10.1080/19336934.2022.2043095

P. Ferree, Maggie Xing, Jenny Zhang, Stefano Di Talia

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引用次数: 0

摘要

蛋白磷酸酶Cdc25Twine活性的下调与果蝇母系到合子转变(MZT)期间细胞周期的重塑有关。本文对Cdc25Twine进行了结构-功能分析。我们用嵌合体证明了Cdc25Twine和Cdc25String的n端区域控制着它们的微分降解动力学。Cdc25Twine的不同区域的缺失揭示了在MZT期间其快速降解所涉及和需要的假设结构域。值得注意的是，Cdc25String中存在一个非常相似的结构域，DNA复制检查点的删除导致Cdc25String和Cdc25Twine的降解动力学相似。最后，我们发现在缺少三号染色体左臂的胚胎中，Cdc25Twine的降解被延迟。因此，我们提出了Cdc25在果蝇MZT上的差异调控模型。

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Structure-function analysis of Cdc25Twine degradation at the Drosophila maternal-to-zygotic transition

ABSTRACT Downregulation of protein phosphatase Cdc25Twine activity is linked to remodelling of the cell cycle during the Drosophila maternal-to-zygotic transition (MZT). Here, we present a structure-function analysis of Cdc25Twine. We use chimeras to show that the N-terminus regions of Cdc25Twine and Cdc25String control their differential degradation dynamics. Deletion of different regions of Cdc25Twine reveals a putative domain involved in and required for its rapid degradation during the MZT. Notably, a very similar domain is present in Cdc25String and deletion of the DNA replication checkpoint results in similar dynamics of degradation of both Cdc25String and Cdc25Twine. Finally, we show that Cdc25Twine degradation is delayed in embryos lacking the left arm of chromosome III. Thus, we propose a model for the differential regulation of Cdc25 at the Drosophila MZT.

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来源期刊

Fly 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.