Sperm viability varies with buffer and genotype in Drosophila melanogaster.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fly Pub Date : 2021-12-01 Epub Date: 2020-12-08 DOI:10.1080/19336934.2020.1837592

Ruijian Guo, Anna-Lena Henke, Klaus Reinhardt

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引用次数: 1

Abstract

Sperm quality, an important male fitness trait, is commonly compared between studies. However, few studies consider how genetic and environmental variation affect sperm quality, even in the genetic model Drosophila melanogaster. Here we show that sperm viability, the proportion of live sperm, differed across the genotypes Oregon-R, Dahomey, and Canton-S by more than 15%, and across buffers (phosphate-buffered saline (PBS), Grace's Medium and Drosophila Ringer solution) by more than 20%. In terms of genotype-buffer pair comparisons, nearly half of the comparisons would produce significant differences in sperm viability (15 in 36), or its temporal decrease in a stress medium (19 in 36). Grace's medium produced the longest-lived sperm in vitro and the smallest differences between genotypes, Drosophila Ringer Solution produced the shortest lifespan and the largest differences. Our results suggest that fly and other sperm researchers would benefit from a standardized protocol of measuring sperm viability.

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黑腹果蝇精子活力随缓冲液和基因型的不同而不同。

精子质量是一项重要的男性健康特征，经常在研究之间进行比较。然而，很少有研究考虑遗传和环境变化如何影响精子质量，即使在遗传模型果蝇中也是如此。在这里，我们发现精子活力(活精子的比例)在基因型Oregon-R、Dahomey和Canton-S之间的差异超过15%，在缓冲液(磷酸盐缓冲盐水(PBS)、Grace培养基和果蝇林格溶液)之间的差异超过20%。在基因型-缓冲对比较方面，近一半的比较会产生显著的精子活力差异(36例中有15例)，或者在应激介质中精子活力降低(36例中有19例)。格雷斯培养基在体外产生的精子寿命最长，基因型之间的差异最小，而果蝇林格溶液产生的精子寿命最短，差异最大。我们的研究结果表明，苍蝇和其他精子研究人员将受益于测量精子活力的标准化协议。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fly 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Fly is the first international peer-reviewed journal to focus on Drosophila research. Fly covers a broad range of biological sub-disciplines, ranging from developmental biology and organogenesis to sensory neurobiology, circadian rhythm and learning and memory, to sex determination, evolutionary biology and speciation. We strive to become the “to go” resource for every researcher working with Drosophila by providing a forum where the specific interests of the Drosophila community can be discussed. With the advance of molecular technologies that enable researchers to manipulate genes and their functions in many other organisms, Fly is now also publishing papers that use other insect model systems used to investigate important biological questions. Fly offers a variety of papers, including Original Research Articles, Methods and Technical Advances, Brief Communications, Reviews and Meeting Reports. In addition, Fly also features two unconventional types of contributions, Counterpoints and Extra View articles. Counterpoints are opinion pieces that critically discuss controversial papers questioning current paradigms, whether justified or not. Extra View articles, which generally are solicited by Fly editors, provide authors of important forthcoming papers published elsewhere an opportunity to expand on their original findings and discuss the broader impact of their discovery. Extra View authors are strongly encouraged to complement their published observations with additional data not included in the original paper or acquired subsequently.