Cell Biology International最新文献_第3页

Ectonucleotidase activity driven by acid ectophosphatase in luminal A MCF-7 breast cancer cells 腔 A MCF-7 乳腺癌细胞中由酸性异磷酸酶驱动的异核苷酸酶活性

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-09-16 DOI: 10.1002/cbin.12237

Marco Antonio Lacerda-Abreu, Bruna dos Santos Mendonça, Gabriela Nestal de Moraes, José Roberto Meyer-Fernandes

{"title":"Ectonucleotidase activity driven by acid ectophosphatase in luminal A MCF-7 breast cancer cells","authors":"Marco Antonio Lacerda-Abreu, Bruna dos Santos Mendonça, Gabriela Nestal de Moraes, José Roberto Meyer-Fernandes","doi":"10.1002/cbin.12237","DOIUrl":"10.1002/cbin.12237","url":null,"abstract":"Ectophosphatases catalyse the hydrolysis of phosphorylated molecules, such as phospho-amino acids, in the extracellular environment. Nevertheless, the hydrolysis of nucleotides in the extracellular environment is typically catalysed by ectonucleotidases. Studies have shown that acid ectophosphatase, or transmembrane-prostatic acid phosphatase (TM-PAP), a membrane-bound splice variant of prostatic acid phosphatase, has ecto-5′-nucleotidase activity. Furthermore, it was demonstrated that ectophosphatase cannot hydrolyse ATP, ADP, or AMP in triple-negative breast cancer cells. In contrast to previous findings in MDA-MB-231 cells, the ectophosphatase studied in the present work displayed a remarkable capacity to hydrolyse AMP in luminal A breast cancer cells (MCF-7). We showed that AMP dose-dependently inhibited p-nitrophenylphosphate (p-NPP) hydrolysis. The p-NPP and AMP hydrolysis showed similar biochemical behaviours, such as increased hydrolysis under acidic conditions and comparable inhibition by NiCl2, ammonium molybdate, and sodium orthovanadate. In addition, this ectophosphatase with ectonucleotidase activity was essential for the release of adenosine and inorganic phosphate from phosphorylated molecules available in the extracellular microenvironment. This is the first study to show that prostatic acid phosphatase on the membrane surface of breast cancer cells (MCF-7) is correlated with cell adhesion and migration.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactate-mitochondrial crosstalk: A new direction in the treatment of sepsis-induced acute kidney injury 乳酸-线粒体串联：治疗败血症所致急性肾损伤的新方向

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-09-09 DOI: 10.1002/cbin.12240

Zhixiong Wu, Wei Qing Liu, Liang Tang, Qiong Yuan, Yaling Li, Hongyu Hu, Xin Luo, Fan Ouyang

引用次数: 0

Lysosomes accumulate at the perinuclear region of muscle cells during chick myogenesis 小鸡肌肉发生过程中溶酶体在肌肉细胞核周区域聚集

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-09-09 DOI: 10.1002/cbin.12238

Kayo Moreira Bagri, Miria Gomes Pereira, Kamila Souto Leichtweis, Jose G. Abreu, Manoel Luis Costa, Claudia Mermelstein

引用次数: 0

OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2 OTUD6B 通过去泛素化 PTK2 来调节 STAT3 磷酸化，从而促进胆管癌的生长。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-27 DOI: 10.1002/cbin.12234

Guoqiang Xing, Hekai Chen, Zhiyue Guo, Yu Cui, Yongyuan Li, Jianwei Shen

{"title":"OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2","authors":"Guoqiang Xing, Hekai Chen, Zhiyue Guo, Yu Cui, Yongyuan Li, Jianwei Shen","doi":"10.1002/cbin.12234","DOIUrl":"10.1002/cbin.12234","url":null,"abstract":"Cholangiocarcinoma (CCA) is a hepatobiliary carcinoma with uncontrolled cell proliferation, poor prognosis, and high mortality. The ovarian tumor structural domain (OTU) containing protein 6B (OTUD6B) belongs to the OTU deubiquitin family and is vital in tumor development. However, its expression and biological function in CCA remain unknown. The expression of OTUD6B in CCA was analyzed using TIMER2.0, UALCAN, and GEO databases. MTT, clonal formation assay, immunofluorescence staining, immunohistochemistry staining, and flow cytometry examined the regulation of OTUD6B on cell proliferation, cycle, and apoptosis. The effects of OTUD6B on tumor volume and weight were assessed using the xenograft tumor model. The activities of PTK2 and STAT3 were detected by western blot and CO-IP. The biological database identified that OTUD6B was upregulated in CCA. In CCA cells, OTUD6B knockdown reduced CCA cell proliferation and promoted apoptosis. Cell cycle analysis indicated that the cycle stopped at the G0/G1 phase after OTU6B downregulation. Furthermore, OTUD6B knockdown resulted in a decrease in tumor volume and weight in xenograft tumor models. Mechanistically, OTUD6B is involved in the deubiquitination of PTK2. PTK2 further affected the phosphorylation of STAT3 thereby regulating the CCA process. Our study demonstrates that OTUD6B knockdown participates in the ubiquitination of PTK2 and phosphorylation of STAT3 to alleviate the process of CCA. These results suggest that OTUD6B may be a potential new strategy for CCA treatment.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142079384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of soluble epoxide hydrolase in the intestine 可溶性环氧化物水解酶在肠道中的作用。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-20 DOI: 10.1002/cbin.12232

Yanbei Ren, Ting Wang, Jiuheng Yin

引用次数: 0

Inhibitors of the mTOR signaling pathway can play an important role in breast cancer immunopathogenesis mTOR 信号通路抑制剂可在乳腺癌免疫发病机制中发挥重要作用。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-20 DOI: 10.1002/cbin.12231

Sulieman I. Shelash Al-Hawary, Farag M. A. Altalbawy, Saade Abdalkareem Jasim, Renuka Jyothi S, Azfar Jamal, Mohammed M. Naiyer, Shriya Mahajan, Hitesh Kalra, Mohammed Abed Jawad, Ahmed Hussein Zwamel

{"title":"Inhibitors of the mTOR signaling pathway can play an important role in breast cancer immunopathogenesis","authors":"Sulieman I. Shelash Al-Hawary, Farag M. A. Altalbawy, Saade Abdalkareem Jasim, Renuka Jyothi S, Azfar Jamal, Mohammed M. Naiyer, Shriya Mahajan, Hitesh Kalra, Mohammed Abed Jawad, Ahmed Hussein Zwamel","doi":"10.1002/cbin.12231","DOIUrl":"10.1002/cbin.12231","url":null,"abstract":"This study explores the critical role of inhibitors targeting the mammalian target of rapamycin (mTOR) signaling pathway in breast cancer research and treatment. The mTOR pathway, a central regulator of cellular processes, has been identified as a crucial factor in the development and progression of breast cancer. The essay explains the complex molecular mechanisms through which mTOR inhibitors, such as rapamycin and its analogs, exert their anticancer effects. These inhibitors can stop cell growth, proliferation, and survival in breast cancer cells by blocking critical signaling pathways within the mTOR pathway. Furthermore, the essay discusses the implications of using mTOR inhibitors as a comprehensive therapeutic strategy. It emphasizes the potential benefits of combining mTOR inhibitors with other treatment approaches to enhance the effectiveness of breast cancer treatment. The evolving landscape of breast cancer research underscores the significance of mTOR as a therapeutic target and highlights ongoing efforts to improve and optimize mTOR inhibitors for clinical use. In conclusion, the essay asserts that inhibitors of the mTOR signaling pathway offer a promising approach in the fight against breast cancer. These inhibitors provide a focused and effective intervention targeting specific dysregulations within the mTOR pathway. As research advances, the integration of mTOR inhibitors into customized combination therapies holds excellent potential for shaping a more effective and personalized approach to breast cancer treatment, ultimately leading to improved outcomes for individuals affected by this complex and diverse disease.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tyrosine kinase inhibitors in the treatment of leptomeningeal carcinomatosis 酪氨酸激酶抑制剂治疗脑膜外癌。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-13 DOI: 10.1002/cbin.12230

Hanyu Ni, Zilan Wang, Yanbing Tang, Jiaye Lu, Zixiang Zhu, Youjia Qiu, Zhouqing Chen, Zhong Wang

{"title":"Tyrosine kinase inhibitors in the treatment of leptomeningeal carcinomatosis","authors":"Hanyu Ni, Zilan Wang, Yanbing Tang, Jiaye Lu, Zixiang Zhu, Youjia Qiu, Zhouqing Chen, Zhong Wang","doi":"10.1002/cbin.12230","DOIUrl":"10.1002/cbin.12230","url":null,"abstract":"Leptomeningeal carcinomatosis (LMC) is a devastating complication of advanced cancers, such as lung cancer and breast cancer, which is usually indicative of a poor prognosis. The current treatments for LMC include palliative care, with others aiming to prolong survival and relieve neurological symptoms. Traditional treatments for LMC include radiotherapy, systemic chemotherapy, and intrathecal injection. Furthermore, the application of molecularly targeted agents, such as antiepidermal growth factor receptor (anti-EGFR), antihuman epidermal growth factor receptor 2 (anti-HER2), and anti-PD-1 monoclonal antibody, have prolonged the survival of LMC patients. Targeted therapy with tyrosine kinase inhibitors has also been proven to be an effective treatment. Tyrosine kinases can be overactive or expressed at high levels in some cancer cells; therefore, the use of tyrosine kinase inhibitors may prevent the activation of tumor-related pathways, preventing cancer cell growth. The EGFR family are cell surface receptors directly related to tumor occurrence with tyrosine kinase activity; it is the most widely used target for tyrosine kinase inhibitors in the treatment of LMC. In this review, we introduced the clinical manifestation and diagnostic criteria of LMC, clarified the treatment mechanism of tyrosine kinase inhibitors for LMC with mutations in EGFR, HER2, or anaplastic lymphoma kinase, reviewed the current application of various generation tyrosine kinase inhibitors in patients with LMC, and discussed new clinical trials and the future directions of tyrosine kinase inhibitor therapy.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

METTL3 mediated ferroptosis in chondrocytes and promoted pain in KOA via HMGB1 m6A modification METTL3 通过 HMGB1 m6A 修饰介导软骨细胞的铁变态反应并促进 KOA 的疼痛。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-11 DOI: 10.1002/cbin.12229

Tianchi Bao, Taiyang Liao, Xuefeng Cai, Binjie Lu, Gaole Dai, Shuai Pei, Yunqing Zhang, Yuwei Li, Bo Xu

{"title":"METTL3 mediated ferroptosis in chondrocytes and promoted pain in KOA via HMGB1 m6A modification","authors":"Tianchi Bao, Taiyang Liao, Xuefeng Cai, Binjie Lu, Gaole Dai, Shuai Pei, Yunqing Zhang, Yuwei Li, Bo Xu","doi":"10.1002/cbin.12229","DOIUrl":"10.1002/cbin.12229","url":null,"abstract":"Methyltransferase-like 3 (METTL3) plays a role in the development of knee osteoarthritis (KOA). However, the mechanism underlying the role of METTL3 in KOA is unclear. This work investigated the effects of MELLT3 on ferroptosis and pain relief in in vitro and in vivo KOA models. Chondrocytes were treated with 10 ng/mL interleukin-1β (IL-1β) or 5 μM Erastin (ferroptosis inducer). IL-1β or Erastin treatment inhibited cell viability and glutathione levels; increased Fe2+, lipid reactive oxygen species and malondialdehyde production; and decreased glutathione peroxidase 4, ferritin light chain and solute carrier family 7 member 11 levels. The overexpression of METTL3 facilitated the N6-methyladenosine methylation of high mobility group box 1 (HMGB1). HMGB1 overexpression reversed the effect of sh-METTL3 on IL-1β-treated chondrocytes. A KOA rat model was established by the injection of monosodium iodoacetate into the joints and successful model establishment was confirmed by haematoxylin and eosin staining and Safranin O/Fast Green staining. METTL3 depletion alleviated cartilage damage, the inflammatory response, ferroptosis and knee pain in KOA model rats, and these effects were reversed by the addition of HMGB1. In conclusion, METTL3 depletion inhibited ferroptosis and the inflammatory response, and ameliorated cartilage damage and knee pain during KOA progression by regulating HMGB1.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD34+ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence CD34+ 基质细胞/骨髓细胞及其在小鼠肺发育中的作用：光镜、免疫荧光、超微结构和扫描电子显微镜证据。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-04 DOI: 10.1002/cbin.12223

Ganesh Dama, Chengxu Xue, Yangxia Zhang, Dezhuang Li, Jinyu Fan, Liang Qiao, Zhihao Xu, Ciqing Yang, Yanli Liu, Mohammad Farris Iman Leong Bin Abdullah, Juntang Lin

{"title":"CD34+ stromal cells/telocytes and their role in mouse lung development: Light microscopy, immunofluorescence, ultrastructural and scanning electron microscopy evidence","authors":"Ganesh Dama, Chengxu Xue, Yangxia Zhang, Dezhuang Li, Jinyu Fan, Liang Qiao, Zhihao Xu, Ciqing Yang, Yanli Liu, Mohammad Farris Iman Leong Bin Abdullah, Juntang Lin","doi":"10.1002/cbin.12223","DOIUrl":"10.1002/cbin.12223","url":null,"abstract":"Telocytes (TCs), a novel type of mesenchymal or interstitial cell with specific, very long and thin cellular prolongations, have been found in various mammalian organs and have potential biological functions. However, their existence during lung development is poorly understood. This study aimed to investigate the existence, morphological features, and role of CD34+ SCs/TCs in mouse lungs from foetal to postnatal life using primary cell culture, double immunofluorescence, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The immunofluorescence double staining profiles revealed positive expression of CD34 and PDGFR-α, Sca-1 or VEGFR-3, and the expression of these markers differed among the age groups during lung development. Intriguingly, in the E18.5 stage of development, along with the CD34+ SCs/TCs, haematopoietic stem cells and angiogenic factors were also significantly increased in number compared with those in the E14.5, E16.5, P0 and P7. Subsequently, TEM confirmed that CD34+ SCs/TCs consisted of a small cell body with long telopodes (Tps) that projected from the cytoplasm. Tps consisted of alternating thin and thick segments known as podomers and podoms. TCs contain abundant endoplasmic reticulum, mitochondria and secretory vesicles and establish close connections with neighbouring cells. Furthermore, SEM revealed characteristic features, including triangular, oval, spherical, or fusiform cell bodies with extensive cellular prolongations, depending on the number of Tps. Our findings provide evidence for the existence of CD34+ SCs/TCs, which contribute to vasculogenesis, the formation of the air‒blood barrier, tissue organization during lung development and homoeostasis.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141888592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DEAD/H-box helicase 11 is transcriptionally activated by Yin Yang-1 and accelerates oral squamous cell carcinoma progression DEAD/H-box螺旋酶11被阴阳-1转录激活，并加速口腔鳞状细胞癌的进展。

IF 3.3 3区生物学

Cell Biology International Pub Date : 2024-08-01 DOI: 10.1002/cbin.12228

Guang Yang, Xin Shi, Meixia Zhang, Kaiwen Wang, Xin Tian, Xiaofeng Wang

{"title":"DEAD/H-box helicase 11 is transcriptionally activated by Yin Yang-1 and accelerates oral squamous cell carcinoma progression","authors":"Guang Yang, Xin Shi, Meixia Zhang, Kaiwen Wang, Xin Tian, Xiaofeng Wang","doi":"10.1002/cbin.12228","DOIUrl":"10.1002/cbin.12228","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) is the most common oral malignancy. DEAD/H-box helicase 11 (DDX11), a DNA helicase, has been implicated in the progression of several cancers. Yet, the precise function of DDX11 in OSCC is poorly understood. The DDX11 expression in OSCC cells and normal oral keratinocytes was evaluated in the Gene Expression Omnibus database (GSE146483 and GSE31853). SCC-4 and CAL-27 cells expressing doxycycline-inducible DDX11 or DDX11 shRNA were generated by lentiviral infection. The role of DDX11 in OSCC cells was determined by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, colony formation assay, flow cytometry assay, TUNEL staining, and western blot. The effects of DDX11 on tumor growth were explored in a xenograft nude mouse model. The relationship between DDX11 and transcription factor Yin Yang-1 (YY1) was researched using the dual luciferase report assay and chromatin immunoprecipitation assay. DDX11 expression was significantly upregulated in OSCC cells. Knockdown of DDX11 inhibited cell proliferation, induced cell cycle arrest, and suppressed PI3K-AKT pathway, while DDX11 overexpression showed opposite effects. The number of apoptotic cells was increased in DDX11 silenced cells. DDX11 upregulation or knockdown accelerated or suppressed tumor growth in vivo, respectively. Moreover, the YY1 bound and activated the DDX11 promoter, resulting in increasing DDX11 expression. Forced expression DDX11 reversed the anticancer effects of YY1 silencing on OSCC cells. DDX11 has tumor-promoting function in OSCC and is transcriptionally regulated by YY1, indicating that DDX11 may serve as a potential target for the OSCC treatment.","PeriodicalId":9806,"journal":{"name":"Cell Biology International","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0