Transcription-Austin最新文献_第4页

A BEN-domain protein and polycomb complex work coordinately to regulate transcription. 一个ben结构域蛋白和多梳复合体协同工作来调节转录。

IF 3.6

Transcription-Austin Pub Date : 2022-02-01 DOI: 10.1080/21541264.2022.2105128

Fredy Kurniawan, Supriya G Prasanth

引用次数: 2

The histone chaperone FACT: a guardian of chromatin structure integrity. 组蛋白伴侣事实:染色质结构完整性的守护者。

IF 3.6

Transcription-Austin Pub Date : 2022-02-01 DOI: 10.1080/21541264.2022.2069995

Célia Jeronimo, François Robert

引用次数: 7

Novel regulation of the transcription factor ZHX2 by N-terminal methylation. n端甲基化对转录因子ZHX2的新调控。

IF 3.6

Transcription-Austin Pub Date : 2022-02-01 DOI: 10.1080/21541264.2022.2079184

Meghan M Conner, Haley V Parker, Daniela R Falcone, Gehoon Chung, Christine E Schaner Tooley

{"title":"Novel regulation of the transcription factor ZHX2 by N-terminal methylation.","authors":"Meghan M Conner, Haley V Parker, Daniela R Falcone, Gehoon Chung, Christine E Schaner Tooley","doi":"10.1080/21541264.2022.2079184","DOIUrl":"https://doi.org/10.1080/21541264.2022.2079184","url":null,"abstract":"N-terminal methylation (Nα-methylation) by the methyltransferase NRMT1 is an important post-translational modification that regulates protein-DNA interactions. Accordingly, its loss impairs functions that are reliant on such interactions, including DNA repair and transcriptional regulation. The global loss of Nα-methylation results in severe developmental and premature aging phenotypes, but given over 300 predicted substrates, it is hard to discern which physiological substrates contribute to each phenotype. One of the most striking phenotypes in NRMT1 knockout (Nrmt1-/-) mice is early liver degeneration. To identify the disrupted signaling pathways leading to this phenotype and the NRMT1 substrates involved, we performed RNA-sequencing analysis of control and Nrmt1-/- adult mouse livers. We found both a significant upregulation of transcripts in the cytochrome P450 (CYP) family and downregulation of transcripts in the major urinary protein (MUP) family. Interestingly, transcription of both families is inversely regulated by the transcription factor zinc fingers and homeoboxes 2 (ZHX2). ZHX2 contains a non-canonical NRMT1 consensus sequence, indicating that its function could be directly regulated by Nα-methylation. We confirmed misregulation of CYP and MUP mRNA and protein levels in Nrmt1-/- livers and verified NRMT1 can methylate ZHX2 in vitro. In addition, we used a mutant of ZHX2 that cannot be methylated to directly demonstrate Nα-methylation promotes ZHX2 transcription factor activity and target promoter occupancy. Finally, we show Nrmt1-/- mice also exhibit early postnatal de-repression of ZHX2 targets involved in fetal liver development. Taken together, these data implicate ZHX2 misregulation as a driving force behind the liver phenotype seen in Nrmt1-/- mice.","PeriodicalId":47009,"journal":{"name":"Transcription-Austin","volume":"13 1-3","pages":"1-15"},"PeriodicalIF":3.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467584/pdf/KTRN_13_2079184.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

BRD4: a general regulator of transcription elongation. BRD4:转录延伸的一般调节因子。

IF 3.6

Transcription-Austin Pub Date : 2022-02-01 Epub Date: 2022-09-01 DOI: 10.1080/21541264.2022.2108302

Elisabeth Altendorfer, Yelizaveta Mochalova, Andreas Mayer

{"title":"BRD4: a general regulator of transcription elongation.","authors":"Elisabeth Altendorfer, Yelizaveta Mochalova, Andreas Mayer","doi":"10.1080/21541264.2022.2108302","DOIUrl":"https://doi.org/10.1080/21541264.2022.2108302","url":null,"abstract":"Transcription elongation by RNA polymerase II (Pol II) has emerged as a regulatory hub in gene expression. A key control point occurs during early transcription elongation when Pol II pauses in the promoter-proximal region at the majority of genes in mammalian cells and at a large set of genes in Drosophila. An increasing number of trans-acting factors have been linked to promoter-proximal pausing. Some factors help to establish the pause, whereas others are required for the release of Pol II into productive elongation. A dysfunction of this elongation control point leads to aberrant gene expression and can contribute to disease development. The BET bromodomain protein BRD4 has been implicated in elongation control. However, only recently direct BRD4-specific functions in Pol II transcription elongation have been uncovered. This mainly became possible with technological advances that allow selective and rapid ablation of BRD4 in cells along with the availability of approaches that capture the immediate consequences on nascent transcription. This review sheds light on the experimental breakthroughs that led to the emerging view of BRD4 as a general regulator of transcription elongation.","PeriodicalId":47009,"journal":{"name":"Transcription-Austin","volume":" ","pages":"70-81"},"PeriodicalIF":3.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40337396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

The pleiotropic roles of SPT5 in transcription. SPT5在转录中的多效性作用。

IF 3.6

Transcription-Austin Pub Date : 2022-02-01 Epub Date: 2022-07-25 DOI: 10.1080/21541264.2022.2103366

Aixia Song, Fei Xavier Chen

引用次数: 12

Oxidative stress induces Ser 2 dephosphorylation of the RNA polymerase II CTD and premature transcription termination. 氧化应激诱导RNA聚合酶II CTD的丝氨酸2去磷酸化和转录过早终止。

IF 3.6

Transcription-Austin Pub Date : 2021-10-01 Epub Date: 2021-12-07 DOI: 10.1080/21541264.2021.2009421

Takashi Yamazaki, Lizhi Liu, James L Manley

{"title":"Oxidative stress induces Ser 2 dephosphorylation of the RNA polymerase II CTD and premature transcription termination.","authors":"Takashi Yamazaki, Lizhi Liu, James L Manley","doi":"10.1080/21541264.2021.2009421","DOIUrl":"https://doi.org/10.1080/21541264.2021.2009421","url":null,"abstract":"The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) consists of YSPTSPS heptapeptide repeats, and the phosphorylation status of the repeats controls multiple transcriptional steps and co-transcriptional events. However, how CTD phosphorylation status responds to distinct environmental stresses is not fully understood. In this study, we found that a drastic reduction in phosphorylation of a subset of Ser2 residues occurs rapidly but transiently following exposure to H2O2. ChIP analysis indicated that Ser2-P, and to a lesser extent Tyr1-P was reduced only at the gene 3' end. Significantly, the levels of polyadenylation factor CstF77, as well as Pol II, were also reduced. However, no increase in uncleaved or readthrough RNA products was observed, suggesting transcribing Pol II prematurely terminates at the gene end in response to H2O2. Further analysis found that the reduction of Ser2-P is, at least in part, regulated by CK2 but independent of FCP1 and other known Ser2 phosphatases. Finally, the H2O2 treatment also affected snRNA 3' processing although surprisingly the U2 processing was not impaired. Together, our data suggest that H2O2 exposure creates a unique CTD phosphorylation state that rapidly alters transcription to deal with acute oxidative stress, perhaps creating a novel \"emergency brake\" mechanism to transiently dampen gene expression.","PeriodicalId":47009,"journal":{"name":"Transcription-Austin","volume":" ","pages":"277-293"},"PeriodicalIF":3.6,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208776/pdf/KTRN_12_2009421.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39952978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

m⁶A RNA modification in transcription regulation. 转录调控中的m6A RNA修饰。

IF 3.6

Transcription-Austin Pub Date : 2021-10-01 DOI: 10.1080/21541264.2022.2057177

Junaid Akhtar, Margot Lugoboni, Guillaume Junion

引用次数: 9

Emerging insights into the function and structure of the Integrator complex. 对整合者复合体功能和结构的新认识。

IF 3.6

Transcription-Austin Pub Date : 2021-10-01 Epub Date: 2022-03-20 DOI: 10.1080/21541264.2022.2047583

Moritz M Pfleiderer, Wojciech P Galej

引用次数: 0

Macromolecular assemblies supporting transcription-translation coupling. 支持转录-翻译耦合的大分子组装。