International Blood Research & Reviews最新文献

{"title":"Study on Malaria and Haemoglobin Interaction in Pregnant Women","authors":"Konne Oroma Ethel, Moore-Igwe Beatrice Wobiarueri, Chukwu Priya Homa, Chukuigwe-Igbere Orokwu Eziaku, Konne Joel Burabari, Konne Felix Eedee","doi":"10.9734/ibrr/2022/v13i230172","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i230172","url":null,"abstract":"Comparative observational study was carried out on the prevalence of malaria among pregnant women attending antenatal clinics in General Hospital, Bori (GHB) and Braithwaite Memorial Specialist Hospital, Port Harcourt (BMSH).  Four hundred women were involved in this study of which two hundred per study location. Pregnant women with pyrexia of unknown origin, HIV and those on anti malarial drugs were excluded. Consents were obtained from participants and confidentiality upheld.  Ethical approval was obtained from the ministry of health and from the selected health facilities. Sample collection was performed according to the recommended reference guideline for phlebotomy. Collected samples were used to assay for Haemoglobin using cyanomethamoglobin method, and malaria parasite using the Giemsa staining technique.  Statistical analysis was performed for percentage, frequency, for descriptive statistics and inferences deduced at p-value=0.05. All statistical analyses were performed using Statistical Package for Social Sciences. Questionnaires were issued to obtain their demographic data.  The prevalence of malaria was high among pregnant women with haemoglobin level 8.0–10.9g/dl from BMSH (17.9%) and GHB (35.9%).  Infection was not dependent on locality at P-value < 0.05. Awareness of  malaria in pregnancy should be supported. Anaemia in pregnancy should be treated and comorbidity of malaria and anaemia in pregnancy should be handled with urgency.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129435187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological Profiles of Eligible Blood Donors at Kenyatta National Hospital, Kenya","authors":"John K. Njenga, Nelson C. Menza, Scholastica G. Mathenge, Jesse N. Githanga","doi":"10.9734/ibrr/2022/v13i230173","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i230173","url":null,"abstract":"Aim: To determine hematological profile of eligible blood donors at Kenyatta National Hospital (KNH), Kenya. \u0000Study Design: Adopted a cross-sectional study. \u0000Place and Duration of Study: Kenyatta National Hospital, between March 2021 and August 2021. \u0000Methodology: This study recruited 202 eligible blood donors comprising of 173 males and 29 females aged 18-57 years. Blood samples (4ml) were drawn from donated units into ethylene diamine tetraacetic acid (EDTA) tube. Hematological parameters were estimated using a complete blood count (CBC) analyzer (Humacount 5D®). A total of eighteen hematological parameters were analyzed. These parameters included; red blood cell (RBC) count, hemoglobin concentration, RBC indices, white blood cell (WBC) count, absolute and differential WBC and platelet (PLT) count. Results were presented in medians and 95% interquartile ranges and compared using Mann–Whitney U test. \u0000Results: The median counts for all hematological parameters were within the accepted reference ranges for the adult urban population in Kenya. The median and interquartile range for total red cell count was 4.9×106/µL [0.74], hemoglobin level was 14.3g/dL[1.8], hematocrit was 44.9% [5.1], white blood count was 4.9×103/µL [1.4] and platelet was 234×103/L [64]. Among the red cell parameters analyzed, male donors had a significantly higher RBC count (P<0.001), hematocrit (P=0.001) and hemoglobin (P<0.001) than female donors. Among white blood cell parameters analyzed, only lymphocytes (P=0.011) were significantly higher in female donors than male donors. Platelet count (P<0.001) was also significantly higher in females than male donors.  \u0000Conclusion: This study showed eligible donors at KNH had significant differences in red cell count, hematocrit, hemoglobin, lymphocytes and platelets between male and female donors. Additionally, it highlighted that some blood donors had hematological parameters below and above the recommended reference ranges. These findings support the need to review the current donor recruitment criteria recommending the inclusion of complete blood count in screening.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114348979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Hepcidin Level among Chronic Kidney Disease Subjects Accessing Healthcare in Braithwaite Memorial Specialist Hospital Port Harcourt","authors":"Lenox-Prince, Tamunonengiye-ofori, Eze, Evelyn Mgbeoma, Mordi, Augustina Ngozi, Jeremiah, Awortu Zacchaeus","doi":"10.9734/ibrr/2022/v13i230171","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i230171","url":null,"abstract":"Hepcidin is the major controller of systemic iron homeostasis and the role of the kidney in regulating hepcidin level is vital in the whole process of iron and hepcidin relationship. This study was aimed at evaluating serum Hepcidin level among Chronic Kidney Disease subjects accessing Healthcare in BMSH Port Harcourt Metropolis. The study was conducted in Port Harcourt at Braithwaite Memorial Specialist Hospital among 55 CKD subjects and 33 normal individuals making up the control group. Subjects were selected randomly and 5mls of blood was collected in plain bottle using venipuncture technique for laboratory assessment of hepcidin. Hepcidin was assayed using competitive ELISA method.  T-test was used to compare the mean difference oh hepcidin between both groups. Result showed that there was a significant difference in hepcidin level between CKD and control groups; 52.00±36.00ng/ml for CKD group and 16.00±13.00ng/ml for control group, p<0.05. This study has shown that CKD has a significant impact in hepcidin level blood and consequently on iron regulation.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126564067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence Rate of Human Immunodeficiency Virus (HIV), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) Co - infection among Expectant Mothers Attending Antenatal Clinic in Rivers State University Teaching Hospital","authors":"B. Moore-Igwe, Beauty Eruchi Echonwere –Uwikor, Ransom Baribefii Jacob, Emmanuel-Nath Ogochukwu","doi":"10.9734/ibrr/2022/v13i230170","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i230170","url":null,"abstract":"The global burden of co-infection with human immunodeficiency virus (HIV), hepatitis B and C virus (HBV and HCV) has a negative impact in Sub-Saharan Africa.When HIV, HBV and HCV co-exist, they become life threatening and with high fatality rate particularly in gestation in which transmission occurs vertically, causing fetal and neonatal hepatitis. The study aimed at examining the occurrence rate of Human Immunodeficiency Virus (HIV), Hepatitis B virus (HBV) and Hepatitis C Virus (HCV) co - infection among expectant mothers attending antenatal clinic in Rivers State University Teaching Hospital (RSUTH). The study population comprised of one hundred and fifty (150) pregnant women. Venous blood was used in the study and screened for hepatitis B surface antigen (HBsAg), anti-HCV, and anti-HIV antibodies using commercially available immunoassay test kits. The prevalence of HCV, HIV and HBsAg among the pregnant subjects in relation to age group 21-30 and 31-40 in the study revealed a seropositive percentage of 0.7% and 1.3%. The other groups, however, showed no positive result among the three viruses. Furthermore, 0.7% of the pregnant women in their first, second and third trimester were co-infected with HCV and HBsAg while 1.3% out of 36.7% and 0.7% out of 61.3% of pregnant women within the age groups 21-30 and 31-40 respectively were seropositive for HIV. In relation to gestational age, it was seen from the study that 0.7% of the pregnant women in their first, second and third trimester were seropositive for HCV and HBsAg respectively, while 2% of the HIV seropositive pregnant women were in their first trimester. The overall seroprevalence of HCV, HIV and HBsAg as revealed in the study showed that infection was found to be 2% respectively among the pregnant women. The reduced prevalence of hepatitis B (HBsAg), hepatitis C (HCV) and human immunodeficiency virus (HIV) infection observed in the study among pregnant women attending antenatal care in the Rivers State University Teaching Hospital may be attributed to the increase in the awareness amongst the general populace in Port Harcourt especially couples about the consequences of sexually transmitted diseases such as HIV, Hepatitis B as well as Hepatitis C. In other words, there is reduction in seroprevalence of HBsAg, HCV and HIV which is premised on the efficacy of sensitization particularly on HBV vaccination and preventive protocols for HIV.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"17 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113976367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant Expression of CD19 in Early T-cell Precursor Lymphoblastic Leukemia- A Diagnostic Challenge","authors":"Neelum Mansoor, S. Maqsood, Sumera M Shaikh","doi":"10.9734/ibrr/2022/v13i130169","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i130169","url":null,"abstract":"A systematic approach is required to diagnose acute leukemia. Most of the cases are satisfactorily diagnosed and categorized into subtypes. However, a few cases pose diagnostic dilemma secondary to immunophenotypic aberrancies which are defined as antigens that are normally restricted to a different lineage and expressed by a neoplastic population while absent from its normal non neoplastic counterpart. We report a rare case of Early T-cell Precursor Lymphoblastic Leukemia with aberrant expression of CD19. A 7-year-old boy referred to our hospital with his cervical lymph node biopsy reported as lymphoproliferative disorder. The patient was COVID-19 positive. Chest X-ray showed mild right sided pleural effusion with huge mediastinal mass. Flow cytometry on peripheral blood used to establish the diagnosis. The case is reported to improve knowledge regarding aberrant expression of markers. Hematopathology teams should be aware of this phenomenon so that appropriate workup can be done to reach correct diagnosis.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133346468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Measurement of Anticoagulant Effect of Acacia Nilotica Aqueous Extract on Normal Human Plasma","authors":"Shaima Naser Saeed, Ahmed Elhadi Elsadig, Tarig A. M. Hamid","doi":"10.9734/ibrr/2022/v13i130168","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i130168","url":null,"abstract":"Aims: To determine the anticoagulant effect of Acacia Nilotica aqueous extract on normal human plasma. \u0000Study Design: This is an experimental study. \u0000Place and Duration of the Study: Department of Haematology, Faculty of Medical Laboratory sciences, Alzaiem Alazhari, Khartoum – Sudan, during the period from July – August 2021. \u0000Methodology: A total of 20 human blood samples were collected for this study from apparently healthy subjects following ethical considerations. Samples were collected in tri-sodium citrate and platelets poor plasma (PPP) were immediately prepared by centrifugation. Aqueous solution of Acacia nilotica was prepared with distilled water (D.W), three concentrations of the solution (50%, 75% and 100%) were prepared. Prothrombin Time (PT) and Activated partial thromboplastin time (APTT) were tested by manual method before and after adding different concentrations of Acacia Nilotica solution. Statistical tests were performed by using SPSS version 16. \u0000Results: The results of study showed that Acacia Nilotica significantly prolonged the PT and APTT results of human plasma (P values for 50%, 75% and 100% solutions  were 0.000, 0.000 and 0.000 while for APTT 0.036, 0.000 and 0.000 for PT respectively). Also the study showed no significant correlation between control and A. nilotica concentrations among coagulation profile, except a significant positive correlation between control and 75% Acacia nilotica on APPT. \u0000Conclusion: Acacia Nilotica solutions had anticoagulant effect in human plasma, and could be nominated as a potential preventive agent for thromboembolic diseases.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115159598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of Free Hemoglobin Percentage in Stored Packed Red Blood Cells Units by Colorimetric Method","authors":"Shima Alaaldeen Alsmanie, A. Elsadig, T. Hamid","doi":"10.9734/ibrr/2022/v13i130167","DOIUrl":"https://doi.org/10.9734/ibrr/2022/v13i130167","url":null,"abstract":"Aims: To measure free hemoglobin percentage in stored packed red blood cells units by colorimetric method. \u0000Study Design: This is a descriptive, hospital based case study. \u0000Place and Duration of Study: Khartoum central blood bank during the period from June to September 2021. \u0000Methodology: A total of thirty-six packed red blood cells blood bags with Citrate-Phosphate-Dextrose-Adenine-one (CPDA-1) stored at 2-6°C for 35 days were withdrawn aseptically on days one; 14, and day 35. Free hemoglobin concentration was measured with Drabkin's method through digital photoelectric colorimeter in which the percentage of hemolysis was calculated, and PH values were measured by a PH meter (ADWA). Mean and standard deviation of each day were calculated by statistical package for social science (SPSS) computer program version 22.0. \u0000Results: The results showed statistically significant elevation in percentages of free plasma hemoglobin with prolongation of storage duration periods; day one compared with day 14 (P = 0.001), day one compared with day 35 (P = 0.001) and day 14 compared with day 35 (P = 0.001). No significant correlation was observed between the degree of hemolysis in day 35 with PH values (P = 0.9). \u0000Conclusion: This study concluded that prolonged storage is associated with elevation of free plasma hemoglobin level indicative of progressive hemolysis.","PeriodicalId":249518,"journal":{"name":"International Blood Research &amp; Reviews","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129755426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}