Bhoopander Giri, R. Rawat, G. Saxena, Preet Manchanda, Qiangsheng Wu, Anuradha Sharma
{"title":"Effect of Rhizoglomus fasciculatum and Paecilomyces lilacinus in the biocontrol of root-knot nematode, Meloidogyne incognita in Capsicum annuum L","authors":"Bhoopander Giri, R. Rawat, G. Saxena, Preet Manchanda, Qiangsheng Wu, Anuradha Sharma","doi":"10.1080/19420889.2021.2025195","DOIUrl":"https://doi.org/10.1080/19420889.2021.2025195","url":null,"abstract":"ABSTRACT Root-knot nematodes possess a major threat to agricultural production of various crops worldwide. The intensive use of chemical nematicides to control plant parasitic nematodes has adverse effects on our environment and human health. Owing to the importance of developing new strategies, an experiment was conducted to reveal the influence of arbuscular mycorrhizal fungus, Rhizoglomus fasciculatum and nematophagous fungus, Paecilomyces lilacinus alone or in combination with various organic amendments such as superphosphate, green and organic manure to control the infection of root-knot, nematode Meloidogyne incognita in a vegetable crop Capsicum annuum. These two fungi along with soil amendments significantly improved plant growth and fruit yield and effectively controlled infection of M. incognita. The dual inoculation of P. lilacinus and R. fasciculatum reduced the number of galls and egg masses, thereby revealing the controlled proliferation of M. incognita infection in C. annuum roots. The beneficial effect of these fungi further increased on supplementation of soil with organic or green manures. Inoculation of C. annuum with these two fungi showed a significant increase in egg parasitization; however, maximum effect was detected on dual inoculation. Amongst the soil amendments, the best response was obtained in case of green manure along with mycorrhizal fungus and P. lilacinus. Present study revealed that nematophagous and AM fungi, in combination with green manure were effective in controlling M. incognita, thus suggesting the use of such agents for biocontrol of plant parasitic nematodes in agriculture.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"15 1","pages":"75 - 87"},"PeriodicalIF":0.0,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48646191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Feelings of Knowing - Fundamental Interoceptive Patterns (FoK-FIP): a magnetic monopole-like \"pure mental\" process fundamental to subjective feelings and self-awareness.","authors":"Holly Pollard-Wright","doi":"10.1080/19420889.2021.2023280","DOIUrl":"https://doi.org/10.1080/19420889.2021.2023280","url":null,"abstract":"<p><p>The Feelings of Knowing - Fundamental Interoceptive Patterns (FoK-FIP) is a transdisciplinary theory developed to explain elusive phenomena suspected to exist that do not easily lend themselves to empirical measurement. The FoK-FIP theory posits that specialized self-generated biomagnetism and \"pure mental\" process share similarities with the hypothetical elementary particle described in particle physics, magnetic monopoles with a magnetic charge. Feelings of Knowing (FoK) are \"awareness charge\" that are self-generated events. Fundamental Interoceptive Patterns (FIP) are restricted oscillatory magnetic fields that are FoK caused phenomena. Further, FoK produces \"cognitive force,\" an observing ego representing specialized interoceptive awareness. Through embodied states, FoK-FIP acts as a \"biological node,\" an informational processing unit in which physiological signals and an observing ego's sensations or feelings are centered. An observing ego cognitively broadcasts using specialized small magnetic signals and four phases of a narrowed range of interoceptive signals. By defining interoceptive signals (i.e., signals of the body's internal state) using FoK-FIP through cognitive broadcasting, an observing ego creates a world it projects around itself. This process is understood through the components map with interoceptive markers (IMs), a novel algorithm based on biological evolution. FoK-FIP-related predictions are described as are empirical studies to test aspects of the theory. The FoK-FIP theory details a path to wellbeing based on a sense of control and capacity for self-care. Mental stability is thought to change as a function of an observing ego's volitional reactions.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":" ","pages":"1-54"},"PeriodicalIF":0.0,"publicationDate":"2022-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39814792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental evidence for extra proton exchange in ribulose 1,5-bisphosphate carboxylase/oxygenase catalysis.","authors":"Camille Bathellier, Guillaume Tcherkez","doi":"10.1080/19420889.2022.2039431","DOIUrl":"https://doi.org/10.1080/19420889.2022.2039431","url":null,"abstract":"<p><p>Despite considerable advances in the past 50 y, the mechanism of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalysis is still not well understood. In particular, the movement and exchange of protons within the active site is not well documented: typically, kinetics of H exchange during the first steps of catalysis, i.e. abstraction of the H3 atom of ribulose 1,5-bisphosphate (RuBP) and enolization, are not clearly established. Here, we took advantage of reaction assays run in heavy water (<sup>2</sup>H<sub>2</sub>O) to monitor the appearance of deuterated RuBP and deuterated products (3-phosphoglycerate and 2-phosphoglycolate) with exact mass LC-MS. Enolization was reversible such that de-enolization generated not only monodeuterated RuBP (<sup>2</sup>H-[H-3]-ribulose 1,5-bisphosphate) but also dideuterated RuBP (<sup>2</sup>H<sub>2</sub>-[H-3,O-3]-ribulose 1,5-bisphosphate). Carboxylation yielded about one half deuterated 3-phosphoglycerate (<sup>2</sup>H-[H-2]-3-phosphoglycerate) and also a small proportion of dideuterated 3-phosphoglycerate (<sup>2</sup>H<sub>2</sub>-[H-2,O-2]-3-phosphoglycerate). Oxygenation generated a small amount of monodeuterated, but no dideuterated, products. (Di)deuterated isotopologue abundance depended negatively on gas concentration. We conclude that in addition to the first step of proton exchange at H3 occurring before gas addition (and thus influenced by the competition between de-enolization and gas addition), there is another proton exchange step between solvent water, active site residues, and the 2,3-enediol(ate) leading to deuterated OH groups in products.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":" ","pages":"68-74"},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39814793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marootpong Pooam, Nathalie Jourdan, Blanche Aguida, Cyril Dahon, Soria Baouz, Colin Terry, Haider Raad, Margaret Ahmad
{"title":"Exposure to 1.8 GHz radiofrequency field modulates ROS in human HEK293 cells as a function of signal amplitude.","authors":"Marootpong Pooam, Nathalie Jourdan, Blanche Aguida, Cyril Dahon, Soria Baouz, Colin Terry, Haider Raad, Margaret Ahmad","doi":"10.1080/19420889.2022.2027698","DOIUrl":"https://doi.org/10.1080/19420889.2022.2027698","url":null,"abstract":"<p><p>The modern telecommunications industry is ubiquitous throughout the world, with a significant percentage of the population using cellular phones on a daily basis. The possible physiological consequences of wireless emissions in the GHz range are therefore of major interest, but remain poorly understood. Here, we show that exposure to a 1.8 GHz carrier frequency in the amplitude range of household telecommunications induces the formation of ROS (Reactive Oxygen Species) in human HEK293 cultured cells. The ROS concentrations detected by fluorescent imaging techniques increased significantly after 15 minutes of RF field exposure, and were localized to both nuclear and cytosolic cellular compartments. qPCR analysis showed altered gene expression of both anti-oxidative (SOD, GPX, GPX, and CAT) and oxidative (Nox-2) enzymes. In addition, multiple genes previously identified as responsive to static magnetic fields were found to also be regulated by RF, suggesting common features in response mechanisms. By contrast, many RF effects showed evidence of hormesis, whereby biological responsivity does not occur linearly as a function of signal amplitude. Instead, biphasic dose response curves occur with 'blind' spots at certain signal amplitudes where no measureable response occurs. We conclude that modulation of intracellular ROS can be a direct consequence of RF exposure dependent on signal frequency and amplitude. Since changes in intracellular ROS may have both harmful and beneficial effects, these could provide the basis for many reported physiological effects of RF exposure.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":" ","pages":"54-66"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39591770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Macrophages and vimentin in tissues adjacent to megaprostheses and mesh in reconstructive surgeries.","authors":"Kunihiro Asanuma, Tomoki Nakamura, Takahiro Iino, Tomohito Hagi, Akihiro Sudo","doi":"10.1080/19420889.2022.2101193","DOIUrl":"https://doi.org/10.1080/19420889.2022.2101193","url":null,"abstract":"<p><p>In reconstructive surgery using artificial materials after wide resection, soft tissues are usually adjacent to metal surfaces or mesh. The purpose of this study was to provide histological evaluation of the soft tissues adjacent to the metal surfaces of megaprostheses and mesh. Tissues from revision surgery of megaprosthesis and from wide resection after recurrent thoracic wall sarcoma were used. Histological analysis was evaluated by hematoxylin/eosin (HE) and Masson's trichrome staining, and by immunohistochemical staining for markers including cluster of differentiation 68 (CD68), vimentin, collagen type and S100A4. Soft tissue adherence to the smooth metal surface of Ti alloy was not observed. On the surface of capsule, CD68- and vimentin-positive cells formed a thin layer. In contrast, soft tissue adherence to a rough-surface cobalt chrome alloy was observed. Capsule was not apparent for this tissue, in which CD68- and vimentin-positive cells were aggregated randomly. In the resected tissues of recurrent chest wall sarcoma, muscles showed connections to connective soft tissues but did not invade to the inside of the mesh. Around the polypropylene mesh, large numbers of CD68- and vimentin-positive cells were seen. On the ePTFE, small numbers of CD68-positive cells were observed, while a larger number of the cells were vimentin positive. High accumulation of S100A4-positive cells was observed at the metal surface and polypropylene surface. Cells were strongly positive for CD68 and vimentin in tissues adjacent to metal and mesh surfaces. Macrophages and vimentin may play important roles in the foreign body reaction to metal and mesh, and so may contribute to encapsulation and fibrosis.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"15 1","pages":"168-181"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10392615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashraf M Mohieldin, Amal Alachkar, John Yates, Surya M Nauli
{"title":"Novel biomarkers of ciliary extracellular vesicles interact with ciliopathy and Alzheimer's associated proteins.","authors":"Ashraf M Mohieldin, Amal Alachkar, John Yates, Surya M Nauli","doi":"10.1080/19420889.2021.2017099","DOIUrl":"https://doi.org/10.1080/19420889.2021.2017099","url":null,"abstract":"<p><p>Ciliary extracellular vesicles (ciEVs), released from primary cilia, contain functional proteins that play an important role in cilia structure and functions. We have recently shown that ciEVs and cytosolic extracellular vesicles (cyEVs) have unique and distinct biomarkers. While ciEV biomarkers have shown some interactions with known ciliary proteins, little is known about the interaction of ciEV proteins with proteins involved in ciliopathy and neurodegenerative disorders. Here, we reveal for the first time the protein-protein interaction (PPI) between the top five ciEVs biomarkers with ciliopathy and Alzheimer disease (AD) proteins. These results support the growing evidence of the critical physiological roles of cilia in neurodegenerative disorders.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"264-269"},"PeriodicalIF":0.0,"publicationDate":"2021-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8726672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39880941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of decreasing population growth-rate on deforestation and population sustainability.","authors":"Gerardo Aquino, Mauro Bologna","doi":"10.1080/19420889.2021.2010394","DOIUrl":"https://doi.org/10.1080/19420889.2021.2010394","url":null,"abstract":"<p><p>We consider the effect of non-constant parameters on the human-forest interaction logistic model coupled with human technological growth introduced in [1]. In recent years in fact, a decrease in human population growth rate has emerged which can be measured to about 1.7% drop per year since 1960 value, which coincides with latest UN projections for next decades up to year 2100 [2]. We therefore consider here the effect of decreasing human population growth-rate on the aforementioned model and we evaluate its effect on the probability of survival of human civilization without going through a catastrophic population collapse. We find that for realistic values of the human population carrying capacity of the earth (measured by the parameter β) this decrease would not affect previous results, leading to a low probability of avoiding a catastrophic collapse. For larger more optimistic values of β instead, a decrease in growth-rate would tilt the probability in favor of a positive outcome, i.e. from 10-20% up to even 95% likelihood of avoiding collapse.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"261-263"},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8726714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39880940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Behaviorist approaches to investigating memory and learning: A primer for synthetic biology and bioengineering.","authors":"Charles I Abramson, Michael Levin","doi":"10.1080/19420889.2021.2005863","DOIUrl":"https://doi.org/10.1080/19420889.2021.2005863","url":null,"abstract":"<p><p>The fields of developmental biology, biomedicine, and artificial life are being revolutionized by advances in synthetic morphology. The next phase of synthetic biology and bioengineering is resulting in the construction of novel organisms (biobots), which exhibit not only morphogenesis and physiology but functional behavior. It is now essential to begin to characterize the behavioral capacity of novel living constructs in terms of their ability to make decisions, form memories, learn from experience, and anticipate future stimuli. These synthetic organisms are highly diverse, and often do not resemble familiar model systems used in behavioral science. Thus, they represent an important context in which to begin to unify and standardize vocabulary and techniques across developmental biology, behavioral ecology, and neuroscience. To facilitate the study of behavior in novel living systems, we present a primer on techniques from the behaviorist tradition that can be used to probe the functions of any organism - natural, chimeric, or synthetic - regardless of the details of their construction or origin. These techniques provide a rich toolkit for advancing the fields of synthetic bioengineering, evolutionary developmental biology, basal cognition, exobiology, and robotics.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"230-247"},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39851953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How fern and fern allies respond to heterogeneous habitat - a case in Yuanjiang dry-hot valley.","authors":"Feng-Chun Yang, Chaya Sarathchandra, Jing-Xin Liu, Hua-Ping Huang, Jian-Yong Gou, Ye Li, Xiao-Ye Mao, Hui-Ting Wen, Jun Zhao, Ming-Fu Yang, Suthathong Homya, Kritana Prueksakorn","doi":"10.1080/19420889.2021.2007591","DOIUrl":"https://doi.org/10.1080/19420889.2021.2007591","url":null,"abstract":"<p><p>The Yuanjiang dry-hot valley features hot and dry climate, low vegetation and soil degradation. It had lush vegetation in the past, but has become degraded in recent decades. Understanding the interrelationship between species and the habitat is necessary to explain this change. In this study, a link between fern and fern allies - a group that is hypersensitive to environmental factors and their circumstances is constructed. Intensive transects and plots were designed to be proxies for extant fern and fern allies, and their habitats. Fifty years of meteorological records of precipitation and temperature along altitude and river running direction (latitudinal) were employed. Alpha and beta diversity are used to access diversity. Species_estimated, Singletons, Uniques, ACE, ICE, and Chao2, which associate to abundance and rarity, are subscribed to the correlation between fern and fern allies, and their ecosystem. Eight species, <i>Selaginella pseudopaleifera, Aleuritopteris squamosa, Adiantum malesianum, Pteris vittata, Davallia trichomanoides, Sinephropteris delavayi, Selaginella jugorum</i>, and <i>Lygodium japonicum</i> are used as indicators of a typical xeric and sun-drying habitat. The results indicate (1) accompanied by dramatically shrinking habitats, fern and fern allies are in very low diversity and abundance, whereas the rarity is relatively high; (2) for fern and fern allies, environmental factors are positive when altitude goes up; and (3) eight indicator species are latitudinally correlated with fern and fern allies along the river running direction.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"248-260"},"PeriodicalIF":0.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39851954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lifting the veil on Bohm's holomovement.","authors":"Andrew Lohrey, Bruce Boreham","doi":"10.1080/19420889.2021.2001157","DOIUrl":"https://doi.org/10.1080/19420889.2021.2001157","url":null,"abstract":"<p><p>In this paper, we argue that Bohm's unbroken and undivided totality he called the holomovement, the title he gave to the concept of the self-organizing universe, is more coherently understood when viewed as universal consciousness. Bohm's understanding of consciousness oscillates around being a quality of local minds and the interconnected totality of the holomovement. We suggest such equivocations impose limitations on Bohm's general holistic framework because they import into his model the limiting restrictions of Cartesian separation and are, therefore, incongruous for use within his holistic model of the holomovement. We also argue that the term 'meaning' has a structural and functional agency appropriate to Bohm's model of the holomovement, while also reflecting the living characteristics of this organic totality that is full of meaning.</p>","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"221-229"},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39800124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}