Communicative and Integrative Biology最新文献_第7页

Experimental evidence for extra proton exchange in ribulose 1,5-bisphosphate carboxylase/oxygenase catalysis. 核酮糖1,5-二磷酸羧化酶/加氧酶催化中额外质子交换的实验证据。

Communicative and Integrative Biology Pub Date : 2022-02-15 eCollection Date: 2022-01-01 DOI: 10.1080/19420889.2022.2039431

Camille Bathellier, Guillaume Tcherkez

{"title":"Experimental evidence for extra proton exchange in ribulose 1,5-bisphosphate carboxylase/oxygenase catalysis.","authors":"Camille Bathellier, Guillaume Tcherkez","doi":"10.1080/19420889.2022.2039431","DOIUrl":"https://doi.org/10.1080/19420889.2022.2039431","url":null,"abstract":"Despite considerable advances in the past 50 y, the mechanism of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalysis is still not well understood. In particular, the movement and exchange of protons within the active site is not well documented: typically, kinetics of H exchange during the first steps of catalysis, i.e. abstraction of the H3 atom of ribulose 1,5-bisphosphate (RuBP) and enolization, are not clearly established. Here, we took advantage of reaction assays run in heavy water (2H2O) to monitor the appearance of deuterated RuBP and deuterated products (3-phosphoglycerate and 2-phosphoglycolate) with exact mass LC-MS. Enolization was reversible such that de-enolization generated not only monodeuterated RuBP (2H-[H-3]-ribulose 1,5-bisphosphate) but also dideuterated RuBP (2H2-[H-3,O-3]-ribulose 1,5-bisphosphate). Carboxylation yielded about one half deuterated 3-phosphoglycerate (2H-[H-2]-3-phosphoglycerate) and also a small proportion of dideuterated 3-phosphoglycerate (2H2-[H-2,O-2]-3-phosphoglycerate). Oxygenation generated a small amount of monodeuterated, but no dideuterated, products. (Di)deuterated isotopologue abundance depended negatively on gas concentration. We conclude that in addition to the first step of proton exchange at H3 occurring before gas addition (and thus influenced by the competition between de-enolization and gas addition), there is another proton exchange step between solvent water, active site residues, and the 2,3-enediol(ate) leading to deuterated OH groups in products.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":" ","pages":"68-74"},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39814793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure to 1.8 GHz radiofrequency field modulates ROS in human HEK293 cells as a function of signal amplitude. 暴露于1.8 GHz射频场可调节人HEK293细胞中的ROS作为信号幅度的函数。

Communicative and Integrative Biology Pub Date : 2022-02-03 eCollection Date: 2022-01-01 DOI: 10.1080/19420889.2022.2027698

Marootpong Pooam, Nathalie Jourdan, Blanche Aguida, Cyril Dahon, Soria Baouz, Colin Terry, Haider Raad, Margaret Ahmad

{"title":"Exposure to 1.8 GHz radiofrequency field modulates ROS in human HEK293 cells as a function of signal amplitude.","authors":"Marootpong Pooam, Nathalie Jourdan, Blanche Aguida, Cyril Dahon, Soria Baouz, Colin Terry, Haider Raad, Margaret Ahmad","doi":"10.1080/19420889.2022.2027698","DOIUrl":"https://doi.org/10.1080/19420889.2022.2027698","url":null,"abstract":"The modern telecommunications industry is ubiquitous throughout the world, with a significant percentage of the population using cellular phones on a daily basis. The possible physiological consequences of wireless emissions in the GHz range are therefore of major interest, but remain poorly understood. Here, we show that exposure to a 1.8 GHz carrier frequency in the amplitude range of household telecommunications induces the formation of ROS (Reactive Oxygen Species) in human HEK293 cultured cells. The ROS concentrations detected by fluorescent imaging techniques increased significantly after 15 minutes of RF field exposure, and were localized to both nuclear and cytosolic cellular compartments. qPCR analysis showed altered gene expression of both anti-oxidative (SOD, GPX, GPX, and CAT) and oxidative (Nox-2) enzymes. In addition, multiple genes previously identified as responsive to static magnetic fields were found to also be regulated by RF, suggesting common features in response mechanisms. By contrast, many RF effects showed evidence of hormesis, whereby biological responsivity does not occur linearly as a function of signal amplitude. Instead, biphasic dose response curves occur with 'blind' spots at certain signal amplitudes where no measureable response occurs. We conclude that modulation of intracellular ROS can be a direct consequence of RF exposure dependent on signal frequency and amplitude. Since changes in intracellular ROS may have both harmful and beneficial effects, these could provide the basis for many reported physiological effects of RF exposure.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":" ","pages":"54-66"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8816398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39591770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Macrophages and vimentin in tissues adjacent to megaprostheses and mesh in reconstructive surgeries. 重建手术中巨噬细胞和巨噬蛋白在大型假体和补片附近组织中的表达。

Communicative and Integrative Biology Pub Date : 2022-01-01 DOI: 10.1080/19420889.2022.2101193

Kunihiro Asanuma, Tomoki Nakamura, Takahiro Iino, Tomohito Hagi, Akihiro Sudo

{"title":"Macrophages and vimentin in tissues adjacent to megaprostheses and mesh in reconstructive surgeries.","authors":"Kunihiro Asanuma, Tomoki Nakamura, Takahiro Iino, Tomohito Hagi, Akihiro Sudo","doi":"10.1080/19420889.2022.2101193","DOIUrl":"https://doi.org/10.1080/19420889.2022.2101193","url":null,"abstract":"In reconstructive surgery using artificial materials after wide resection, soft tissues are usually adjacent to metal surfaces or mesh. The purpose of this study was to provide histological evaluation of the soft tissues adjacent to the metal surfaces of megaprostheses and mesh. Tissues from revision surgery of megaprosthesis and from wide resection after recurrent thoracic wall sarcoma were used. Histological analysis was evaluated by hematoxylin/eosin (HE) and Masson's trichrome staining, and by immunohistochemical staining for markers including cluster of differentiation 68 (CD68), vimentin, collagen type and S100A4. Soft tissue adherence to the smooth metal surface of Ti alloy was not observed. On the surface of capsule, CD68- and vimentin-positive cells formed a thin layer. In contrast, soft tissue adherence to a rough-surface cobalt chrome alloy was observed. Capsule was not apparent for this tissue, in which CD68- and vimentin-positive cells were aggregated randomly. In the resected tissues of recurrent chest wall sarcoma, muscles showed connections to connective soft tissues but did not invade to the inside of the mesh. Around the polypropylene mesh, large numbers of CD68- and vimentin-positive cells were seen. On the ePTFE, small numbers of CD68-positive cells were observed, while a larger number of the cells were vimentin positive. High accumulation of S100A4-positive cells was observed at the metal surface and polypropylene surface. Cells were strongly positive for CD68 and vimentin in tissues adjacent to metal and mesh surfaces. Macrophages and vimentin may play important roles in the foreign body reaction to metal and mesh, and so may contribute to encapsulation and fibrosis.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"15 1","pages":"168-181"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10392615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Novel biomarkers of ciliary extracellular vesicles interact with ciliopathy and Alzheimer's associated proteins. 纤毛细胞外囊泡的新生物标志物与纤毛病和阿尔茨海默病相关蛋白相互作用。

Communicative and Integrative Biology Pub Date : 2021-12-25 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.2017099

Ashraf M Mohieldin, Amal Alachkar, John Yates, Surya M Nauli

引用次数: 7

Effect of decreasing population growth-rate on deforestation and population sustainability. 人口增长率下降对森林砍伐和人口可持续性的影响。

Communicative and Integrative Biology Pub Date : 2021-12-18 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.2010394

Gerardo Aquino, Mauro Bologna

{"title":"Effect of decreasing population growth-rate on deforestation and population sustainability.","authors":"Gerardo Aquino, Mauro Bologna","doi":"10.1080/19420889.2021.2010394","DOIUrl":"https://doi.org/10.1080/19420889.2021.2010394","url":null,"abstract":"We consider the effect of non-constant parameters on the human-forest interaction logistic model coupled with human technological growth introduced in [1]. In recent years in fact, a decrease in human population growth rate has emerged which can be measured to about 1.7% drop per year since 1960 value, which coincides with latest UN projections for next decades up to year 2100 [2]. We therefore consider here the effect of decreasing human population growth-rate on the aforementioned model and we evaluate its effect on the probability of survival of human civilization without going through a catastrophic population collapse. We find that for realistic values of the human population carrying capacity of the earth (measured by the parameter β) this decrease would not affect previous results, leading to a low probability of avoiding a catastrophic collapse. For larger more optimistic values of β instead, a decrease in growth-rate would tilt the probability in favor of a positive outcome, i.e. from 10-20% up to even 95% likelihood of avoiding collapse.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"261-263"},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8726714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39880940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Behaviorist approaches to investigating memory and learning: A primer for synthetic biology and bioengineering. 研究记忆和学习的行为主义方法:合成生物学和生物工程入门。

Communicative and Integrative Biology Pub Date : 2021-12-14 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.2005863

Charles I Abramson, Michael Levin

{"title":"Behaviorist approaches to investigating memory and learning: A primer for synthetic biology and bioengineering.","authors":"Charles I Abramson, Michael Levin","doi":"10.1080/19420889.2021.2005863","DOIUrl":"https://doi.org/10.1080/19420889.2021.2005863","url":null,"abstract":"The fields of developmental biology, biomedicine, and artificial life are being revolutionized by advances in synthetic morphology. The next phase of synthetic biology and bioengineering is resulting in the construction of novel organisms (biobots), which exhibit not only morphogenesis and physiology but functional behavior. It is now essential to begin to characterize the behavioral capacity of novel living constructs in terms of their ability to make decisions, form memories, learn from experience, and anticipate future stimuli. These synthetic organisms are highly diverse, and often do not resemble familiar model systems used in behavioral science. Thus, they represent an important context in which to begin to unify and standardize vocabulary and techniques across developmental biology, behavioral ecology, and neuroscience. To facilitate the study of behavior in novel living systems, we present a primer on techniques from the behaviorist tradition that can be used to probe the functions of any organism - natural, chimeric, or synthetic - regardless of the details of their construction or origin. These techniques provide a rich toolkit for advancing the fields of synthetic bioengineering, evolutionary developmental biology, basal cognition, exobiology, and robotics.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"230-247"},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39851953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

How fern and fern allies respond to heterogeneous habitat - a case in Yuanjiang dry-hot valley. 蕨类及其同族对异质生境的响应——以沅江干热河谷为例

Communicative and Integrative Biology Pub Date : 2021-12-13 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.2007591

Feng-Chun Yang, Chaya Sarathchandra, Jing-Xin Liu, Hua-Ping Huang, Jian-Yong Gou, Ye Li, Xiao-Ye Mao, Hui-Ting Wen, Jun Zhao, Ming-Fu Yang, Suthathong Homya, Kritana Prueksakorn

{"title":"How fern and fern allies respond to heterogeneous habitat - a case in Yuanjiang dry-hot valley.","authors":"Feng-Chun Yang, Chaya Sarathchandra, Jing-Xin Liu, Hua-Ping Huang, Jian-Yong Gou, Ye Li, Xiao-Ye Mao, Hui-Ting Wen, Jun Zhao, Ming-Fu Yang, Suthathong Homya, Kritana Prueksakorn","doi":"10.1080/19420889.2021.2007591","DOIUrl":"https://doi.org/10.1080/19420889.2021.2007591","url":null,"abstract":"The Yuanjiang dry-hot valley features hot and dry climate, low vegetation and soil degradation. It had lush vegetation in the past, but has become degraded in recent decades. Understanding the interrelationship between species and the habitat is necessary to explain this change. In this study, a link between fern and fern allies - a group that is hypersensitive to environmental factors and their circumstances is constructed. Intensive transects and plots were designed to be proxies for extant fern and fern allies, and their habitats. Fifty years of meteorological records of precipitation and temperature along altitude and river running direction (latitudinal) were employed. Alpha and beta diversity are used to access diversity. Species_estimated, Singletons, Uniques, ACE, ICE, and Chao2, which associate to abundance and rarity, are subscribed to the correlation between fern and fern allies, and their ecosystem. Eight species, Selaginella pseudopaleifera, Aleuritopteris squamosa, Adiantum malesianum, Pteris vittata, Davallia trichomanoides, Sinephropteris delavayi, Selaginella jugorum, and Lygodium japonicum are used as indicators of a typical xeric and sun-drying habitat. The results indicate (1) accompanied by dramatically shrinking habitats, fern and fern allies are in very low diversity and abundance, whereas the rarity is relatively high; (2) for fern and fern allies, environmental factors are positive when altitude goes up; and (3) eight indicator species are latitudinally correlated with fern and fern allies along the river running direction.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"248-260"},"PeriodicalIF":0.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39851954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lifting the veil on Bohm's holomovement. 揭开玻姆全息运动的面纱。

Communicative and Integrative Biology Pub Date : 2021-11-22 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.2001157

Andrew Lohrey, Bruce Boreham

引用次数: 3

Infrared light therapy relieves TLR-4 dependent hyper-inflammation of the type induced by COVID-19. 红外光治疗可缓解COVID-19诱导的TLR-4依赖性高炎症。

Communicative and Integrative Biology Pub Date : 2021-09-15 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.1965718

Blanche Aguida, Marootpong Pooam, Margaret Ahmad, Nathalie Jourdan

{"title":"Infrared light therapy relieves TLR-4 dependent hyper-inflammation of the type induced by COVID-19.","authors":"Blanche Aguida, Marootpong Pooam, Margaret Ahmad, Nathalie Jourdan","doi":"10.1080/19420889.2021.1965718","DOIUrl":"https://doi.org/10.1080/19420889.2021.1965718","url":null,"abstract":"The leading cause of mortality from COVID-19 infection is respiratory distress due to an exaggerated host immune response, resulting in hyper-inflammation and ensuing cytokine storms in the lungs. Current drug-based therapies are of limited efficacy, costly, and have potential negative side effects. By contrast, photobiomodulation therapy, which involves periodic brief exposure to red or infrared light, is a noninvasive, safe, and affordable method that is currently being used to treat a wide range of diseases with underlying inflammatory conditions. Here, we show that exposure to two 10-min, high-intensity periods per day of infrared light causes a marked reduction in the TLR-4 dependent inflammatory response pathway, which has been implicated in the onset of cytokine storms in COVID-19 patients. Infrared light exposure resulted in a significant decline in NFkB and AP1 activity as measured by the reporter gene assay; decreased expression of inflammatory marker genes IL-6, IL-8, TNF-alpha, INF-alpha, and INF-beta as determined by qPCR gene expression assay; and an 80% decline in secreted cytokine IL6 as measured by ELISA assay in cultured human cells. All of these changes occurred after only 48 hours of treatment. We suggest that an underlying cellular mechanism involving modulation of ROS may downregulate the host immune response after Infrared Light exposure, leading to decrease in inflammation. We further discuss technical considerations involving light sources and exposure conditions to put these observations into potential clinical use to treat COVID-19 induced mortality.","PeriodicalId":39647,"journal":{"name":"Communicative and Integrative Biology","volume":"14 1","pages":"200-211"},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39440521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Stem cells part of the innate and adaptive immune systems as a therapeutic for Covid-19. 干细胞是先天免疫系统和适应性免疫系统的一部分，可用于治疗Covid-19。

Communicative and Integrative Biology Pub Date : 2021-09-10 eCollection Date: 2021-01-01 DOI: 10.1080/19420889.2021.1965356

Greg Maguire

引用次数: 6