综合性期刊最新文献

{"title":"The recurrence and mortality risk in Luminal A breast cancer patients who lived in high pollution area.","authors":"Pimwarat Srikummoon, Patrinee Traisathit, Wimrak Onchan, Chagkrit Ditsatham, Natthapat Thongsak, Nawapon Nakharutai, Salinee Thumronglaohapun, Titaporn Supasri, Phonpat Hemwan, Imjai Chitapanarux","doi":"10.1371/journal.pone.0335140","DOIUrl":"https://doi.org/10.1371/journal.pone.0335140","url":null,"abstract":"<p><p>Luminal A is the most common subtype of breast cancer and has the best prognosis comparing to the others. The association between air pollution and survival of breast cancer have been reported but not specific to this subtype. We examined pollutant distributions over a decade in upper Northern Thailand, the area of high average annual particulate matter levels, and their impact on the mortality and recurrence risks of patients with luminal A breast cancer. Retrospective data of 1,305 luminal A breast cancer patients diagnosed from 2003 to 2018 were enrolled to this study. Cox proportional hazard models were used to identify factors associated with mortality and recurrence risks including all known risk factors and the annually averaged concentrations of pollutants. On multivariable analysis; metastatic stage (adjusted hazard ratio (aHR) =10.50; 95% confidence interval (95%CI): 7.23-15.25), smoking history (aHR = 1.72; 95% CI: 1.14-2.60), and age ≥ 50 years old (aHR = 1.46; 95% CI: 1.13-1.90) were significant factors influencing mortality risk. Factors contributing to recurrence risk included metastatic stage (aHR) 4.96 (95% CI: 2.78-8.83) and exposure to the time-updated local concentration of PM10 > 55 µg/m3 (aHR = 1.68; 95% CI: 1.16-2.45). Exposure to air pollutants is one of the detrimental factors affected to recurrence and mortality in luminal A subtype breast cancer.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0335140"},"PeriodicalIF":2.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FoxO3a regulated by miR-150-5p promotes the pyroptosis of macrophages in atherosclerosis.","authors":"Ping-Yu Cai, Mei-Mei Li, Shu-Han Chen, Yan-Li Zheng, Jun Li, De-Hong Huang, Jing-Ru Du, Hui-Li Lin","doi":"10.1371/journal.pone.0327075","DOIUrl":"https://doi.org/10.1371/journal.pone.0327075","url":null,"abstract":"<p><strong>Background: </strong>This study uses tissue exosome analysis to explore the role of miR-150-5p and its downstream genes in atherosclerosis (AS), an area where the functional mechanisms and pathophysiological significance of exosomal miR-150-5p remain poorly understood.</p><p><strong>Methods: </strong>Exosomes from AS mouse vascular tissue were analyzed to identify miR-150-5p target genes. Dual luciferase assays validated miRNA-target interactions, while RT-qPCR and Western blot assessed FoxO3a expression. RNA interference studies determined FoxO3a's role in pyroptosis. In vivo efficacy of the miR-150-5p inhibitor was evaluated using HE, Masson staining, and immunofluorescence.</p><p><strong>Results: </strong>In AS tissue exosomes, miR-150-5p levels increased whereas FoxO3a levels decreased. miR-150-5p regulated FoxO3a, enhancing macrophage pyroptosis. The miR-150-5p inhibitor reduced ox-LDL-induced RAW264.7 injury and pyroptosis by improving cell viability, decreasing LDH levels, and downregulating pyroptosis related proteins (Caspase-1, NLRP3, GSDMD-N). FoxO3a knockdown weakened the inhibitor's effects on NLRP3/GSDMD-mediated pyroptosis. In Apoe-/- mice, the inhibitor upregulated FoxO3a/ARC and suppressed pyroptosis signaling.</p><p><strong>Conclusion: </strong>This study advances understanding of miR-150-5p-mediated pyroptosis and highlights the potential of miR-150-5p inhibitors in combating AS.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0327075"},"PeriodicalIF":2.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From protection to pollution: Evaluating environmental and human health risks of acaricide use in dairy farming in Kenya.","authors":"Kevin W Maina, Martin C Parlasca, Elizaphan E J O Rao","doi":"10.1371/journal.pone.0333694","DOIUrl":"https://doi.org/10.1371/journal.pone.0333694","url":null,"abstract":"<p><p>Sustainable intensification of livestock production relies critically on effective disease management, yet the environmental implications of current practices remain poorly understood. The study was designed to evaluate the efficacy of acaricide use in tick control in Kenya's dairy sector affects environmental and human health risks. Using original survey data from dairy farmers and a two-stage least square (2SLS) approach, the results found that farmers' adaptation to perceived ineffective tick treatment leads to potentially harmful practices. Twenty percent of farmers improperly rotate acaricides, while 66% under-apply recommended doses. Despite 65% using protective gear, 29% report adverse health effects. Our estimates show that improper acaricide group rotation increases the environmental and human health risks by 35%. The study highlights important trade-offs between animal health management and environmental and human health objectives, suggesting a need to reform current disease prevention approaches to balance productivity gains with environmental sustainability in developing countries.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0333694"},"PeriodicalIF":2.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial dysfunction-related metabolite methylmalonic acid is associated with decreased cognitive performance.","authors":"Yige Liu, Jiaxin Wang, Pengyan Wu, Fan Tang, Wanlu Guo, Ruishan Li, Xinran Wang, Yiying Zhang, Shaohong Fang","doi":"10.1371/journal.pone.0332987","DOIUrl":"https://doi.org/10.1371/journal.pone.0332987","url":null,"abstract":"<p><strong>Purpose: </strong>Methylmalonic acid (MMA), a mitochondrial metabolite derived from propionate metabolism, has been implicated in fatal neurodegeneration in children with congenital methylmalonic acidemia. However, its clinical relevance in chronic cognitive decline remains poorly understood. This study aims to investigate the association between MMA levels, its metabolic coenzyme vitamin B12, and cognitive impairment in an elderly population.</p><p><strong>Patients and methods: </strong>The cohort comprised 2,762 participants aged 60 and older, who underwent assessments using the Digit Symbol Substitution Test (DSST), Animal Fluency test (AFT), Consortium to Establish a Registry for Alzheimer's disease (CERAD) Immediate Recall (CERAD-IR), CERAD Delayed Recall (CERAD-DR), with low cognitive performance defined as the bottom quartile. Generalized linear regression models were applied to explore potential associations. Additionally, differentially expressed genes (DEGs) in MMA-related propionate metabolic process were identified from the RNA expression profiles in frontal cortex from 56 Alzheimer's disease and 44 non-dementia controls.</p><p><strong>Results: </strong>Among the 2,762 participants, with a mean age of 69.2 years and 53.8% female, elevated MMA levels were significantly associated with an increased likelihood of low cognitive performance across multiple tests: DSST (OR = 1.95; 95% CI: 1.41-2.73), AFT (OR = 1.51; 95% CI: 1.01-2.25), CERAD-IR (OR = 1.58; 95% CI: 1.19-2.09), and CERAD-DR (OR = 1.56; 95% CI: 1.12-2.18). High serum vitamin B12 levels were associated with an increased likelihood of impaired CERAD-IR scores, although dietary B12 supplementation did not correlate with cognitive performance. Stratified analyses revealed a stronger relationship between MMA and cognitive decline in males, individuals over 75 years, and those with elevated serum and dietary vitamin B12 levels. DEG analysis identified genes related to MMA synthesis and mitochondrial function in cognitively impaired individuals, with significant enrichment in mitochondrial propionate metabolism pathways.</p><p><strong>Conclusion: </strong>The findings indicate that elevated MMA levels are linked to cognitive decline in older adults, potentially through mitochondrial dysfunction. These results underscore the clinical importance of MMA in the context of chronic cognitive impairment.</p>","PeriodicalId":20189,"journal":{"name":"PLoS ONE","volume":"20 10","pages":"e0332987"},"PeriodicalIF":2.6,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mutation detection method of discontinuous structures in rock strata based on drilling parameters.","authors":"Xin Sun, Jingyi Cheng, Zhijun Wan, Zhiqing Guo, Shuruo Zhang, Shuheng Wang","doi":"10.1038/s41598-025-20423-w","DOIUrl":"https://doi.org/10.1038/s41598-025-20423-w","url":null,"abstract":"<p><p>Discontinuous structures in coal mine roadway roofs, such as rock interfaces and joint fractures, are critical factors leading to surrounding rock instability. The use of Measurement While Drilling (MWD) technology to identify geological formations has become a growing trend. However, there is still a lack of rock structure recognition methods that offer high accuracy, efficiency, and strong generalizability. Therefore, this study acquired four drilling parameters including thrust, torque, modulation specific energy (SEM), and rock drillability assessment (RDA) through drilling experiments. By leveraging the Bayes algorithm, which has high precision, efficiency, and low cost, a change point detection model for drilling parameters was established, and a multi-parameter fusion criterion was proposed for identifying rock structures. The results show that for single rock interface identification, the errors of thrust, torque, SEM, and RDA were 13.3 mm, 4.6 mm, 4.4 mm, and 18.3 mm, respectively. For multiple rock interface identification, the recognition rates were 83.3%, 100.0%, 66.7%, and 83.3%, respectively. Moreover, the absolute value of the magnitude index (SLP) at the interface location was generally the highest among all change points. In multi-change-point detection, the SLP threshold should be set at ± 0.2. It is worth noting that the SLP value is correlated with data fluctuation intensity; greater fluctuation leads to higher SLP values at change points. This study contributes significantly to enabling intelligent perception of rock structures and improving the quality of rock mass control.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36447"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing the normal and epileptic output of a neural mass model based on cyclic-small gain theorem.","authors":"Sina Hosseini, Abolfazl Yaghmaei, Fariba Bahrami, Mohammad Javad Yazdanpanah","doi":"10.1038/s41598-025-20315-z","DOIUrl":"https://doi.org/10.1038/s41598-025-20315-z","url":null,"abstract":"<p><p>This study presents a new application of the Cyclic-Small Gain Theorem (CSGT) to analyze the behavior of a Neural Mass Model (NMM), which is utilized to study brain activity related to epilepsy. The model is reformulated as an interconnected network of dynamic subsystems, which made CSGT applicable to this context. It is shown that whenever the CSGT conditions are satisfied, the model is input-to-state stable, and epilepsy cannot occur. Furthermore, the proposed method guarantees normal activity even with increased amplitude of noise, as long as the stability conditions of CSGT are verified. In this way, system instability can be considered indicative of the occurrence of epilepsy. The behavior of an interconnected network consisting of two epileptic columns and a healthy column is analyzed using the proposed method to study the propagation of epileptic activity between regions. The results show that, when the healthy column satisfies the CSGT conditions, it remains stable and unaffected by its epileptic counterparts. The study emphasizes the importance of the CSGT as a protective measure against epileptic activity and provides insights for designing control interventions using electric brain stimulation.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36412"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A clinical scoring system to predict tuberculous lymphadenitis in settings with high disease burden.","authors":"Ermias Teklehaimanot Yefter, Asefa Adimasu Taddese, Getahun Mengistu Tessema, Miteku Andualem Limenih, Alemante Tafese Beyna, Addisu Minaye Dejen, Destaye Shiferaw Alemu","doi":"10.1038/s41598-025-20322-0","DOIUrl":"https://doi.org/10.1038/s41598-025-20322-0","url":null,"abstract":"<p><p>Limited diagnostic service availability challenges the timely diagnosis and treatment of tuberculous lymphadenitis, the most common extrapulmonary tuberculosis in endemic areas. To address this, our study aimed to develop a clinical scoring rule for estimating the likelihood of tuberculous lymphadenitis, designed as a simple and easy-to-apply classification tool to facilitate timely diagnosis. We used a cross-sectional study to collect data from 1364 patients with lymphadenopathy. A prediction model was developed by incorporating predictors from multivariable logistic regression analysis. Optimal cutoff point was determined using Youden's Index. Performance was evaluated using discrimination and calibration. Internal validation and clinical utility were assessed using bootstrapping and decision curve analysis, respectively. A total of 790 patients (57.9%, 95% CI 55.3%-60.5%) were diagnosed with tuberculous lymphadenitis. Sex, duration, size, location, and consistency of lymphadenopathy, presence of pain, sinus tract, systemic symptoms, and erythrocyte sedimentation rate were predictors incorporated to develop the scoring rule. With a discrimination power of 96.9% (95% CI 96.1%, 97.8%), the clinical scoring rule using these predictors showed a sensitivity, specificity, and accuracy of 93.7%, 89.7%, and 92%, respectively. It demonstrated internal validity with near-zero optimism coefficients for various performance metrics. Using easily accessible clinical parameters, we developed a scoring rule that reliably predicts tuberculous lymphadenitis. Besides its use in clinical settings for making timely clinical decisions, the scoring rule is a valuable candidate for community-level screening, facilitating early case detection.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36439"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences and fingerprints of ESBL-producing E. coli from chicken faeces.","authors":"Nisa Sipahi, Yasemin Numanoglu Cevik","doi":"10.1038/s41598-025-14114-9","DOIUrl":"https://doi.org/10.1038/s41598-025-14114-9","url":null,"abstract":"<p><p>E. coli is an important source of β-lactam resistance. Extended Spectrum β-Lactamase (ESBL) producing E. coli and their phylogenetic transmission are an important problem for health. On the other hand, MALDI-TOF MS allows sensitive and specific applications in food safety and many clinical studies, and it is also a method approved by the Food and Drug Administration (FDA) for microbial identification. We thought that the protein phenotypic character of the bacteria might provide data on resistance. This study aims to investigate β-lactamases in E. coli and to examine the differences in protein properties of ESBL-producing bacteria. In the present study that is the first report for Duzce (Türkiye), 28.6% of 122 isolates were identified as Chicken Faeces E. coli (CFEC)-ESBL. bla_CTX-M, bla_CTX-M-1, bla_CTX-M-15, bla_SHV, bla_TEM, bla_OXA-10, bla_CIT and bla_MOX genes were determined with PCR and bla_CTX-M gene was detected with the highest rate (88.5%). At least one of the resistance genes was detected in the phenotype screening tests, except one of the isolates (CFEC-ESBL-90). On the other hand, CFEC-ESBL-38 contained bla_CTX-M-15 and the fact that this isolate was the only atypical ESBL strain with indole (-) and lac (-) characteristics among all isolates explains the highest variance (41%). It was also different from other Principle Component Analysis (PCA) components. Moreover, this isolate had a high degree of similarity (87%; CCI) with the other isolate (CFEC-ESBL-90), which had low similarity to CFEC-ESBLs. The study determined the differences and similarities between E. coli isolates with all PCA analyses. Our findings indicate that the ESBL group generally differed from susceptible strains, and the isolates had some heterogeneities and homogeneities. As a result, phyloproteomic analyses with MALDI-TOF MS are considered beneficial in characterising phenotypic bacterial behaviour. Despite that, there were some limitations in the study that need to be solved with further research.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36310"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous-wave and mode-locked Bi-doped fiber laser at 1.7 μm.","authors":"Ali Roohforouz, M R K Soltanian, Pin Long, Nitika Vaish, Lawrence R Chen","doi":"10.1038/s41598-025-20559-9","DOIUrl":"https://doi.org/10.1038/s41598-025-20559-9","url":null,"abstract":"<p><p>We investigate continuous-wave (CW) and mode-locked (ML) operation of bismuth-doped fiber (BDF) lasers at 1.7 μm. We compare the slope efficiency (SE) of the Figure-of-9 (Fo9) and linear cavity configurations for CW operation and find that the linear cavity exhibits a slightly higher SE. We demonstrate that the Fo9 configuration can also support mode-locked operation. We observe multi-pulse operation and can control the number of output pulses by adjusting the pump power.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36455"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental investigation of al₂o₃ nanoparticle-enhanced natural ester oil as a biodegradable liquid dielectric for transformers.","authors":"S Marisargunam, T Mariprasath, P Saraswathi, Mohit Bajaj, Milkias Berhanu Tuka","doi":"10.1038/s41598-025-16210-2","DOIUrl":"https://doi.org/10.1038/s41598-025-16210-2","url":null,"abstract":"<p><p>Mineral oil has been widely used as a liquid dielectric in transformers, serving as both an insulating and heat dissipation medium. However, its poor biodegradability, low fire resistance, and environmental concerns limit its long-term viability. To address these challenges, this study investigates natural ester oil (NEO) as an eco-friendly, biodegradable alternative, offering enhanced insulation and thermal performance. The research focuses on evaluating NEO's key characteristics, aging behavior, and failure probability, with and without Al₂O₃ nanoparticle enhancement. A 60-day accelerated thermal aging test at 100 °C was conducted to assess the degradation resistance of NEO compared to mineral oil. The effects of partial discharge, creepage failure, and electrical stress were analyzed, and a Weibull statistical model was applied to predict insulation failure probabilities. Experimental results confirm that NEO demonstrates superior breakdown strength, lower degradation rate, and higher fire safety. The addition of Al₂O₃ nanoparticles significantly improves dielectric properties, reduces viscosity, and enhances the thermal stability of NEO, making it more suitable for transformer applications. This research establishes nano-enhanced NEO as a superior alternative to mineral oil, offering higher insulation performance, improved thermal durability, and enhanced fire safety. The findings advocate for its adoption as a sustainable, high-performance dielectric for modern transformers, promoting both reliability and environmental compliance.</p>","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"15 1","pages":"36311"},"PeriodicalIF":3.9,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145313616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}