综合性期刊最新文献

{"title":"Author Correction: Potential of artificial intelligence in reducing energy and carbon emissions of commercial buildings at scale","authors":"Chao Ding, Jing Ke, Mark Levine, Jessica Granderson, Nan Zhou","doi":"10.1038/s41467-025-63487-y","DOIUrl":"https://doi.org/10.1038/s41467-025-63487-y","url":null,"abstract":"<p>Correction to: <i>Nature Communications</i>; https://doi.org/10.1038/s41467-024-50088-4, published online 14 July 2024</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"23 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DKC1-mediated pseudouridylation of rRNA targets hnRNP A1 to sustain IRES-dependent translation and ATF4-driven metabolic adaptation","authors":"Anamika Gupta,&nbsp;Mohit Bansal,&nbsp;Jane Ding,&nbsp;Suman Karki,&nbsp;Madhuparna Pandit,&nbsp;Sunil Sudarshan,&nbsp;Han-Fei Ding","doi":"10.1126/sciadv.adv9401","DOIUrl":"10.1126/sciadv.adv9401","url":null,"abstract":"<div >The pseudouridine synthase DKC1 regulates internal ribosome entry site (IRES)–dependent translation and is up-regulated in cancers by the MYC family of oncogenes. The functional significance of DKC1 up-regulation and the mechanistic connection between pseudouridylation and IRES-mediated translation remain poorly understood. Here, we report that DKC1 drives an ATF4-mediated transcriptional program that supports amino acid metabolism and stress adaptation. We identify hnRNP A1, an IRES trans-acting factor, as a critical downstream mediator of DKC1 in sustaining ATF4 expression and IRES-dependent translation. Mechanistically, DKC1-mediated pseudouridylation at two specific 28<i>S</i> ribosomal RNA sites is crucial for maintaining hnRNP A1 protein expression. In turn, hnRNP A1 binds and stabilizes ATF4 messenger RNA, preferentially promoting IRES-dependent translation of ATF4 variant 1. Furthermore, cellular stress induces hnRNP A1, which is necessary for stress-induced ATF4 protein expression. Collectively, our findings uncover an MYC-driven DKC1-hnRNP A1 axis that links IRES-dependent translation and ATF4-mediated metabolic adaptation, thereby supporting cancer cell survival under metabolic stress during tumor progression.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 35","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv9401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A unified model of transient poration induced by antimicrobial peptides","authors":"Amy Rice, Andriana C. Zourou, Myriam L. Cotten, Richard W. Pastor","doi":"10.1073/pnas.2510294122","DOIUrl":"https://doi.org/10.1073/pnas.2510294122","url":null,"abstract":"Membrane active peptides (MAPs) represent a diverse group of agents that disrupt the integrity of lipid membranes. One class of MAPs, antimicrobial peptides (AMPs), destroy bacteria by transiently porating the bacterial membrane causing leakage of cellular contents. Transient leakage is classified as “graded,” where all vesicles in a population leak partially, or “all-or-none,” where some vesicles leak completely. However, the molecular interactions underlying transient leakage have eluded experimental determination. Here, dye leakage experiments with the AMP piscidin 1 (P1) show that graded leakage can be converted to all-or-none by simply adding a defect-promoting lysophospholipid. Molecular dynamics simulations demonstrate that area stress arising from membrane asymmetry decreases the energy of pore formation and is highly lipid dependent. Furthermore, lipids and peptides translocate the bilayer through these pores, leading to area-relaxed states where poration is highly unfavorable. Even pores too small to leak dye relieve area stress; they are the “none” component of all-or-none release. These observations lead to development of a quantitative model where graded and all-or-none leakage are treated as a continuum explained by a single mechanism that accounts for the local peptide concentration and probabilities of different pore sizes. This unified model accurately reproduces the P1 dye leakage data and provides an explanation for varying pore energy, size, and probability within the framework of asymmetry-driven poration. This model is expected to be applicable to other MAPs, including cell-penetrating peptides, and could provide a framework for designing peptides with greater cellular specificity, a long-sought outcome.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"30 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CSF total tau as a proxy of synaptic degeneration","authors":"Carolina Soares, Bruna Bellaver, Pamela C. L. Ferreira, Guilherme Povala, Cristiano Schaffer Aguzzoli, João Pedro Ferrari-Souza, Hussein Zalzale, Firoza Z. Lussier, Francieli Rohden, Sarah Abbas, Guilherme Bauer-Negrini, Douglas Teixeira Leffa, Andréa Lessa Benedet, Rebecca Langhough, Tobey J. Betthauser, Bradley T. Christian, Rachael E. Wilson, Dana L. Tudorascu, Pedro Rosa-Neto, Thomas K. Karikari, Henrik Zetterberg, Kaj Blennow, Eduardo R. Zimmer, Sterling C. Johnson, Tharick A. Pascoal","doi":"10.1038/s41467-025-63545-5","DOIUrl":"https://doi.org/10.1038/s41467-025-63545-5","url":null,"abstract":"<p>Cerebrospinal fluid (CSF) total tau (t-tau) is considered a biomarker of neuronal degeneration alongside brain atrophy and fluid neurofilament light chain protein (NfL) in biomarker models of Alzheimer’s disease (AD). However, previous studies show that CSF t-tau correlates strongly with synaptic dysfunction/degeneration biomarkers like neurogranin (Ng) and synaptosomal-associated protein 25 (SNAP25). Here, we compare the association between CSF t-tau and synaptic degeneration and axonal/neuronal degeneration biomarkers in cognitively unimpaired and impaired groups from two independent cohorts. We observe a stronger correlation between CSF t-tau and synaptic biomarkers than neurodegeneration biomarkers in both groups. Synaptic biomarkers explain a greater proportion of variance in CSF t-tau levels compared to neurodegeneration biomarkers. Notably, CSF t-tau levels are elevated in individuals with abnormalities only in synaptic biomarkers, but not in individuals with abnormalities only in neurodegeneration biomarkers. Our findings suggest that CSF t-tau is a closer proxy for synaptic degeneration than for axonal/neuronal degeneration.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"21 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photo-homogenization assisted segregation easing technique (PHASET) for highly efficient and stable wide-bandgap perovskite solar cells","authors":"Liming Du, Fangfang Cao, Rui Meng, Yueying Zhang, Junchuan Zhang, Zhiyu Gao, Cong Chen, Can Li, Dewei Zhao, Jichun Ye, Zhen Li, Chuanxiao Xiao","doi":"10.1038/s41467-025-63176-w","DOIUrl":"https://doi.org/10.1038/s41467-025-63176-w","url":null,"abstract":"<p>Wide-bandgap (WBG) perovskite solar cells (PSCs) can exceed the Shockley–Queisser limit in tandem solar cells (TSCs), but phase segregation under continuous illumination limits their stability. Using in-situ microscopic characterizations, we investigate the dynamics of photon-induced phase segregation. Initial light soaking drives iodide diffusion into a metastable state, but continued redistribution increases the phase separation energy barrier, resulting in a more stable, segregation-resistant state. Inspired by stabilization methods in silicon photovoltaics, we develop the Photo-Homogenization Assisted Segregation Easing Technique (PHASET), which combines light soaking with 2-ThEABr surface passivation to suppress halide segregation. PHASET enhances efficiency and stability, enabling an efficiency of 20.23% for 1.79 eV WBG-PSCs, with 97% of the initial efficiency retained after 1200 hours of continuous illumination. Integration with a 1.25 eV narrow-bandgap subcell results in a two-terminal all-perovskite TSC with 28.64% efficiency, retaining 77% of its initial performance after 1200 hours of maximum power point tracking.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"55 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced enzyme stability at the interphase of water-oil for continuous-flow olefin epoxidation","authors":"Ming Zhang, Minran Wang, Yumeng Mi, Ting Li, Dawei Hou, Haitao Li, Xili Tong, Tingting Zhang, Huimin Yi, Hengquan Yang","doi":"10.1038/s41467-025-63476-1","DOIUrl":"https://doi.org/10.1038/s41467-025-63476-1","url":null,"abstract":"<p>The practical applications of enzymes often require their immobilization for multiple recycling or long-term running. However, practically efficient enzyme immobilization methods are lacking. Herein, we present an enzyme immobilization approach by engineering a porous “interphase” between water and oil around the surfaces of Pickering emulsion droplets. The designed “interphase” consists of a porous, nanometer-thick silica shell serving as a scaffold to incorporate enzymes. Within this “interphase”, enzymes can simultaneously be in contact with enzyme-preferred aqueous microenvironment and the oil phase containing organic reactants. The porous “interphase” with its tunable structure and properties allows modulation of transport of reactants, crudely akin to a cell membrane, and of local concentration of reactants. As a proof of the concept, we showcase that our “interphase” strategy is very effective in immobilization of <i>Candida antarctica</i> lipase B (CALB) for continuous-flow olefin epoxidation. Long-term stabilization (800 h), 16-fold increase in catalysis efficiency relative to batch reactions, and 99% H₂O₂ utilization efficiency are achieved. The integration of unique microenvironment and hydrophobic pores of the “interphase” is found to be crucial for such excellent performances, practically providing the most efficient enzymatic epoxidation system. This strategy opens an avenue for the design of efficient and sustainable biocatalytic processes.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"18 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abnormal mitochondrial structure and function in brown adipose tissue of SLC35A4-MP knockout mice","authors":"Andréa L. Rocha,&nbsp;Christian Schmedt,&nbsp;Guy Perkins,&nbsp;Antonio Pinto,&nbsp;Jolene K. Diedrich,&nbsp;Huanqi Shan,&nbsp;Kaja Plucińska,&nbsp;Eduardo Vieira de Souza,&nbsp;Joan M. Vaughan,&nbsp;Mark Foster,&nbsp;Srinath C. Sampath,&nbsp;Srihari C. Sampath,&nbsp;Paul Cohen,&nbsp;Mark H. Ellisman,&nbsp;Alan Saghatelian","doi":"10.1126/sciadv.ads7381","DOIUrl":"10.1126/sciadv.ads7381","url":null,"abstract":"<div >Uncovering the role of upstream open reading frames (uORFs) challenges conventional views of one protein per messenger RNA and reveals the capacity of some uORFs to encode microproteins that contribute to cellular biology and physiology. This study explores the functional role of a recently identified mitochondrial microprotein, SLC35A4-MP, in the brown adipose tissue of mice. Our findings reveal dynamic regulation of SLC35A4-MP expression during primary brown adipocyte differentiation in vitro and during cold exposure or high-fat diet (HFD)–induced obesity in mice. Using a knockout mouse model, we show that loss of SLC35A4-MP disrupts mitochondrial lipid composition, decreasing cardiolipins and phosphatidylethanolamine in brown adipose tissue from HFD-fed mice. SLC35A4-MP deficiency also impairs mitochondrial activity, alters mitochondrial number and morphology, and promotes inflammation. Knockout mice accumulate acylcarnitines during cold exposure, indicating defective fatty acid oxidation. These findings reveal SLC35A4-MP as a previously unrecognized microprotein in regulating mitochondrial function and tissue lipid metabolism, adding to the growing list of functional endogenous microproteins.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 35","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads7381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voice identity invariance by anterior temporal lobe neurons","authors":"Margherita Giamundo,&nbsp;Régis Trapeau,&nbsp;Etienne Thoret,&nbsp;Luc Renaud,&nbsp;Thomas Brochier,&nbsp;Pascal Belin","doi":"10.1126/sciadv.adv7033","DOIUrl":"10.1126/sciadv.adv7033","url":null,"abstract":"<div >The ability to recognize speakers by their voice despite acoustical variation plays a substantial role in primate social interactions. Although neurons in the macaque anterior temporal lobe (ATL) show invariance to face viewpoint, whether they also encode abstract representations of caller identity is not known. Here, we demonstrate that neurons in the voice-selective ATL of two macaques support invariant voice identity representations through dynamic population-level coding. These representations minimize neural distances across different vocalizations from the same individual while preserving distinct trajectories for different callers. This structure emerged from the coordinated activity of the broader neuronal ensemble, although a small subset of highly identity-selective neurons carried high identity information. Our findings provide a neural basis for voice identity recognition in primates and highlight the ATL as a key hub for integrating perceptual voice features into higher-level identity representations.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 35","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv7033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropeptide dynamics coordinate layered plasticity mechanisms adapting Drosophila circadian behavior to changing environment","authors":"Abhishek Chatterjee,&nbsp;Joydeep De,&nbsp;Béatrice Martin,&nbsp;Elisabeth Chélot,&nbsp;Ping Zhong,&nbsp;François Rouyer","doi":"10.1126/sciadv.adt7168","DOIUrl":"10.1126/sciadv.adt7168","url":null,"abstract":"<div >The <i>Drosophila</i> brain contains distinct circadian oscillators responsible for generating the morning and evening bouts of locomotor activity in light-dark cycles. We lack a mechanistic understanding of how environmental changes reshape the resulting bimodal rest-activity pattern. Here, we uncover a seasonal switch mechanism that remodels the evening bout of activity. Under temperate summer-like conditions, levels of the pigment-dispersing factor (PDF) neuropeptide diminish, triggering a cascade. Lowered PDF receptor (PDFR) signaling disinhibits glycogen synthase kinase 3/SHAGGY to advance the evening output, and in parallel, weakens the siesta-promoting <i>vc</i>DN1p-SIF<i>amide</i> axis to expand the evening peak. Under these conditions, <i>vc</i>DN1p takes over the evening pacemaker role by exerting control over the dorsal lateral oscillator neurons. Our findings elucidate how environment-induced changes in PDFR signaling tip the balanced output of the clock network, aligning daily rhythms with seasonal time. Neuropeptide-driven parallel adjustment of clock circuitry and clock protein functioning likely represents a conserved strategy, enabling animals to adapt their daily behavior to seasonal changes.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 35","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt7168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TIGER: A tdTomato in vivo genome-editing reporter mouse for investigating precision-editor delivery approaches","authors":"Samuel W. Du, Grazyna Palczewska, Zhiqian Dong, Julie C. Lauterborn, Balasankara Reddy Kaipa, Alexander L. Yan, Rafał Hołubowicz, Siyoung Ha, Paul Z. Chen, Christine M. Gall, Gulab Zode, David R. Liu, Krzysztof Palczewski","doi":"10.1073/pnas.2506257122","DOIUrl":"https://doi.org/10.1073/pnas.2506257122","url":null,"abstract":"In vivo genome editing has the potential to address many inherited and environmental disorders. However, a major hurdle for the clinical translation of genome editing is safe, efficient delivery to disease-relevant tissues. A modality-agnostic reporter animal model that facilitates rapid, precise, and quantifiable assessment of functional delivery and editing could greatly enhance the evaluation and translation of delivery technologies. Here, we present the development of the tdTomato in vivo genome-editing reporter (TIGER) mouse, a reporter strain that harbors an integrated and constitutively expressed mutated tdTomato gene in the <jats:italic toggle=\"yes\">Polr2a</jats:italic> locus. The mutations (Q115X, Q357X) abolish fluorescence, but successful adenine base editing (ABE) or prime editing (PE) restores tdTomato fluorescence. This mouse model facilitates the tissue- and cell type–specific assessment of genome editing agent delivery. We describe several editing strategies validated in vitro and demonstrate efficient ABE and PE in vivo using viral and nonviral delivery vectors targeting four cell types within the mouse eye: the retinal pigment epithelium, photoreceptors, Müller glia, and the trabecular meshwork. We show direct editing characterization in the ocular tissues via in vivo and ex vivo two-photon confocal microscopy and verify the spectral and fluorescence lifetime properties of tdTomato reporter in other mouse tissues. Additionally, we demonstrate successful adeno-associated virus (AAV)-mediated PE of extraocular tissues, including hepatocytes, skeletal muscle, and brain neurons by intravenous injection. Thus, the TIGER mouse facilitates the direct development, comparison, and optimization of delivery platforms for efficient and productive ABE or PE broadly applicable in vivo across multiple tissues tested in this study.","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"23 1","pages":""},"PeriodicalIF":11.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}