其他最新文献

{"title":"Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.","authors":"Yan Zheng, Baichen Sun, Zhiling Qu","doi":"10.1080/20565623.2025.2489328","DOIUrl":"10.1080/20565623.2025.2489328","url":null,"abstract":"<p><strong>Objective: </strong>Transcriptome and proteome analyses may yield inconsistent predictions regarding tumor prognosis. The clinical and pathological significance of ASPM expression in lung adenocarcinoma (LUAD) remains unclear. This study investigates the expression and prognostic value of ASPM, focusing on its role in chemotherapy outcomes.</p><p><strong>Methods: </strong>We analyzed the prognostic relevance of ASPM using bioinformatics, immunohistochemical staining of LUAD tissue microarrays, and proteomics data. Further, in vitro experiments were conducted to evaluate the effects of ASPM overexpression on cell proliferation and sensitivity to cisplatin.</p><p><strong>Results: </strong>Bioinformatics analysis revealed that ASPM's prognostic significance differed between transcriptomic and proteomic datasets. Immunohistochemistry showed that high ASPM expression predicted improved overall survival only in LUAD patients undergoing chemotherapy, not in those without. Proteomics analysis identified ASPM-related signatures enriched in cell cycle and mitosis pathways. In vitro, ASPM overexpression promoted tumor cell proliferation and enhanced cisplatin-induced cytotoxicity.</p><p><strong>Conclusion: </strong>ASPM exhibits a dual role in LUAD prognosis, acting as a marker for improved chemotherapy outcomes while promoting tumor proliferation. These findings underscore ASPM's potential as a therapeutic target and predictive marker for personalized treatment in LUAD.</p>","PeriodicalId":12568,"journal":{"name":"Future Science OA","volume":"11 1","pages":"2489328"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11988246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking protein kinase targeting advances: integrating QSAR into machine learning for kinase-targeted drug discovery.","authors":"Rand Shahin, Sawsan Jaafreh, Yusra Azzam","doi":"10.1080/20565623.2025.2483631","DOIUrl":"10.1080/20565623.2025.2483631","url":null,"abstract":"<p><p>Protein kinases are vital drug targets, yet designing selective inhibitors is challenging, compounded by resistance and kinome complexity. This review explores Quantitative Structure-Activity Relationship (QSAR) modeling for kinase drug discovery, focusing on integrating traditional QSAR with machine learning (ML)-CNNs, RNNs-and structural data. Methods include structural databases, docking, and deep learning QSAR. Key findings show ML-integrated QSAR significantly improves selective inhibitor design for CDKs, JAKs, PIM kinases. The IDG-DREAM challenge exemplifies ML's potential for accurate kinase-inhibitor interaction prediction, outperforming traditional methods and enabling inhibitors with enhanced selectivity, efficacy, and resistance mitigation. QSAR combined with advanced computation and experimental data accelerates kinase drug discovery, offering transformative precision medicine potential. This review highlights deep learning-enhanced QSAR's novelty in automating feature extraction and capturing complex relationships, surpassing traditional QSAR, while emphasizing interpretability and experimental validation for clinical translation.</p>","PeriodicalId":12568,"journal":{"name":"Future Science OA","volume":"11 1","pages":"2483631"},"PeriodicalIF":2.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein targeting to Starch 2 and the plastidial phosphorylase 1 revealed protein-protein interactions with photosynthesis proteins in yeast two-hybrid screenings.","authors":"Sidratul Nur Muntaha, Joerg Fettke","doi":"10.1080/15592324.2025.2470775","DOIUrl":"10.1080/15592324.2025.2470775","url":null,"abstract":"<p><p>Starch metabolism in plants involves a complex network of interacting proteins that work together to ensure the efficient synthesis and degradation of starch. These interactions are crucial for regulating the balance between energy storage and release, adapting to the plant's developmental stage and environmental conditions. Several studies have been performed to investigate protein-protein interactions (PPIs) in starch metabolism complexes, yet it remains impossible to unveil all of the PPIs in this highly regulated process. This study uses yeast-two-hybrid (Y2H) screening against the Arabidopsis leaf cDNA library to explore PPIs, focusing on the starch-granule-initiating protein named Protein Targeting to Starch 2 (PTST2, At1g27070) and the protein involved in starch and maltodextrin metabolism, namely, plastidial phosphorylase 1 (PHS1, EC 2.4.1.1). More than 100 positive interactions were sequenced, and we found chloroplastidial proteins to be putative interacting partners of PTST2 and PHS1. Among them, photosynthetic proteins were discovered. These novel interactions could reveal new roles of PTST2 and PHS1 in the connection between starch metabolism and photosynthesis. This dynamic interplay between starch metabolism and other chloroplast functions highlights the importance of starch as both an energy reservoir and a regulatory component in the broader context of plant physiology and adaptation.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2470775"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune infiltration landscape and potential drug-targeted implications for hepatocellular carcinoma with 'progression/hyper-progression' recurrence.","authors":"Jing-Xuan Xu, Yue-Xiang Su, Yuan-Yuan Chen, Yi-Yue Huang, Zu-Shun Chen, Yu-Chong Peng, Lu-Nan Qi","doi":"10.1080/07853890.2025.2456113","DOIUrl":"10.1080/07853890.2025.2456113","url":null,"abstract":"<p><strong>Background and aims: </strong>Hepatocellular carcinoma (HCC) recurrence was previously characterized into four types, and patients with progression/hyper-progression recurrence (type III-IV) have an extremely poor prognosis. However, the immune background of resectable HCC, particularly in patients who experience recurrence, remains underexplored. Therefore, this study aimed to describe the immune landscape of resectable HCC, especially postoperative type III-IV recurrent HCC, and explore potential immune-targeted anti-relapse strategies for treated populations.</p><p><strong>Methods: </strong>The differences in gene expression in patients with recurrent HCC (type I-II (solitary or multi-intrahepatic oligo recurrence) vs. type III-IV) were investigated using bulk sequencing. Multiple immune infiltration methods (single-sample gene set enrichment analysis (GSEA), Microenvironment Cell Populations-counter and ESTIMATE) were used, and patients were divided into four groups to identify four distinct immune subtypes: immune-enrichment/matrix-poor (IE1), immune-enrichment/matrix-rich (IE2), immune intermediate/matrix-rich (ITM) and immune desert/matrix-poor (ID). Co-expression and protein interaction analyses were used to identify characteristic genes in ITM closely associated with type III-IV recurrence, which was matched with drug targets for Huaier granules (HG) and lenvatinib. Virtual docking was used to identify potential therapeutic targets, and the results were verified using single-nuclei RNA sequencing and histological analysis.</p><p><strong>Results: </strong>ITM was closely related to type III-IV recurrence and exhibited immunotherapy potential. The potential efficacy of inhibiting CCNA2, VEGFA, CXCL8, PLK2, TIMP1, ITGB2, ALDOA, ANXA5 and CSK in ITM reversal was determined. Molecular docking demonstrated that the proteins of these genes could bind to HG or lenvatinib. The immunohistochemical findings demonstrated differential VEGFA (<i>p</i> < .01) and PLK2 (<i>p</i> < .001) expression in ITM type and ID in type III-IV recurrent HCC.</p><p><strong>Conclusions: </strong>Three primary immunotypes of resectable HCC (IE2, ITM and ID) were identified, and HG and lenvatinib could potentially overcome immune checkpoint blockade (ICB) resistance in ITM patients with HCC, particularly those classified as type III-IV.</p>","PeriodicalId":93874,"journal":{"name":"Annals of medicine","volume":"57 1","pages":"2456113"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11774162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the therapeutic horizons of spesolimab: a review of off-label applications for inflammatory skin diseases.","authors":"Hanlin Zhang, Jia Zhou, Keyun Tang, Xinyi Zhang, Hongzhong Jin","doi":"10.1080/09546634.2025.2460582","DOIUrl":"10.1080/09546634.2025.2460582","url":null,"abstract":"<p><strong>Purpose: </strong>This review aims to outline the crucial role of IL-36 signaling in inflammatory skin diseases and summarize the therapeutic potential of spesolimab. Our goal is to provide insights into the off-label applications of spesolimab and future directions for its use in treating other challenging skin diseases.</p><p><strong>Materials and methods: </strong>We conducted a comprehensive literature search across PubMed, Embase, Web of Science, MEDLINE, Scopus, and the Cochrane Library to identify relevant studies. For RCTs, we additionally searched the ClinicalTrials.gov database.</p><p><strong>Results: </strong>In this review, we examine its off-label applications for conditions such as palmoplantar pustulosis, acrodermatitis continua of Hallopeau, hidradenitis suppurativa, pyoderma gangrenosum, and acute generalized exanthematous pustulosis. This review also explores the role of IL-36 in the pathophysiology of these disorders and discusses how spesolimab may address the limitations of current therapies for refractory cases. Randomized controlled trials and case reports are summarized to highlight the efficacy and tolerability of spesolimab across various inflammatory skin conditions. We highlight the challenges presented by the absence of standardized treatment guidelines and the need for larger clinical trials.</p><p><strong>Conclusions: </strong>This review underscores the potential of spesolimab to enhance treatment strategies for inflammatory skin diseases.</p>","PeriodicalId":94235,"journal":{"name":"The Journal of dermatological treatment","volume":"36 1","pages":"2460582"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving soybean drought tolerance via silicon-induced changes in growth, physiological, biochemical, and root characteristics.","authors":"Malik Muhammad Abdullah, Ejaz Ahmad Waraich, Muhammad Ahmad, Saddam Hussain, Hafiz Naeem Asghar, Arslan Haider, Usman Zulfiqar, Zahoor Ahmad, Walid Soufan, Pv Vara Prasad, Ivica Djalovic","doi":"10.1080/15592324.2025.2465232","DOIUrl":"10.1080/15592324.2025.2465232","url":null,"abstract":"<p><p>Drought-induced osmotic stress is a significant constraint to soybean growth and yield, necessitating the development of effective mitigation strategies. Silicon acts as an important strategy to mitigate the negative stress effects of drought stress. The study was aimed to evaluate the potential of soil-applied silicon in alleviating drought stress in soybean. Two field capacities were tested: control (85% FC) and drought (50% FC), with four silicon application rates (0, 100, 200, and 300 kg ha^-1) applied at sowing. Drought stress significantly affected the morphological parameters in soybean as plant height, leaf area, and water potential were reduced by 25%, 20%, and 36%, respectively, while root length increased as compared to control-85% FC. However, drought stress reduced root density, surface area, and biomass as compared to control-85% FC. Additionally, drought reduced photosynthetic rates, chlorophyll a and b levels, and stomatal conductance, while increasing malondialdehyde and hydrogen peroxide. The natural plant defense system was upregulated, with increased activity of phenolics, soluble proteins, and antioxidant enzymes like catalase, superoxide dismutase, and peroxidase. However, silicon applications, especially at 200 kg ha^-1, significantly alleviated the negative effects of drought stress by improving morphophysiological and biochemical traits in soybeans. Compared to the control, Si₂₀₀ increased plant height, root length, photosynthetic rate, and water potential by 22%, 39%, 23%, and 17%, respectively, as compared to control. Furthermore, silicon reduced malondialdehyde and hydrogen peroxide levels by 21% and 10%, enhancing plant resilience. Silicon supplementation also boosted biochemical attributes, with total soluble proteins, phenolics, and antioxidant enzyme activities increasing by 30%, 55%, 19%, 24%, and 31%, respectively, under drought conditions. In crux, silicon at 200 kg ha^-1 effectively mitigated the effects of drought stress in soybean, becoming a more sustainable approach to sustain crop yield and food security.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2465232"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whether the transient hair shedding phase exist after minoxidil treatment and does it predict treatment efficacy? A retrospective study in androgenetic alopecia patients.","authors":"Lingbo Bi, Haili Kan, Jing Wang, Yunbu Ding, Yuanbo Huang, Chaofan Wang, Yimei Du, Changpei Lu, Min Zhao, Weiling Sun, Tong Su, Weixin Fan","doi":"10.1080/09546634.2025.2480739","DOIUrl":"10.1080/09546634.2025.2480739","url":null,"abstract":"<p><strong>Purpose: </strong>Minoxidil is a routinely used drug in treating multiple hair disorders. This study aimed to investigate the facticity of the temporal increase in the hair shedding amount after topical use of minoxidil.</p><p><strong>Materials and methods: </strong>We selected 49 patients who used 2% or 5% minoxidil topically to treat androgenetic alopecia for 24 weeks. The amount of hair shedding was recorded every four weeks before and after the treatment. The BASP classification and trichoscopy test results were also recorded before and after the treatment. The relative amount of hair shedding (RAHS) was defined as the recorded number of hair shedding after normalization. The correlation between the maximum RAHS (MRAHS) and the sulfotransferase activity as well as the therapeutic effect was calculated.</p><p><strong>Results: </strong>A temporary increase in the amount of hair shedding was detected in the first 12 weeks. This increase has a longer duration in patients treated with 2% minoxidil compared to 5%. Its severity was correlated with the improvement of trichoscopy tests only in patients with 5% minoxidil but not in 2%. However, both patients in the 2% and 5% minoxidil groups had a significant association between the MRAHS and the improvement in BASP classification.</p><p><strong>Conclusion: </strong>The amount of hair shedding increases temporarily after the topical minoxidil use, the level of which is a potential treatment efficacy.</p>","PeriodicalId":94235,"journal":{"name":"The Journal of dermatological treatment","volume":"36 1","pages":"2480739"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cumulative associations between health behaviours, mental well-being, and health over 30 years.","authors":"Tiia Kekäläinen, Johanna Ahola, Emmi Reinilä, Tiina Savikangas, Marja-Liisa Kinnunen, Tuuli Pitkänen, Katja Kokko","doi":"10.1080/07853890.2025.2479233","DOIUrl":"https://doi.org/10.1080/07853890.2025.2479233","url":null,"abstract":"<p><strong>Background: </strong>Both the number of risky health behaviours and the duration of exposure to these behaviours over time may increase the risk of later adverse outcomes. This study examined cumulative associations of risky health behaviours with both positive and negative aspects of mental well-being and health. It has a uniquely long follow-up period of over 30 years, from early adulthood to the beginning of late adulthood.</p><p><strong>Materials and methods: </strong>The data were from the Jyväskylä Longitudinal Study of Personality and Social Development. The participants represent the Finnish age cohort born in 1959. This study utilized data collected at ages 27 (1986), 36 (1995), 42 (2001), 50 (2009), and 61 (2020-2021) (<i>n</i> = 206-326). Risk scores indicating the current number of risky behaviours of smoking, heavy alcohol consumption, and physical inactivity and their temporal accumulation over time were calculated. The associations of risk scores with mental well-being (depressive symptoms, psychological well-being) and health (self-rated health, number of metabolic risk factors) from age 36 onwards were analyzed with linear multilevel models adjusted for gender and education.</p><p><strong>Results: </strong>More current risky behaviours were associated with more depressive symptoms (<i>B</i> = 0.10, <i>p</i> = 0.032), lower psychological well-being (<i>B</i> = -0.10, <i>p</i> = 0.010), lower self-rated health (<i>B</i> = -0.45, <i>p</i> < 0.001), and more metabolic risk factors (<i>B</i> = 0.53, <i>p</i> = 0.013). The associations of temporal risk scores with the outcomes were even stronger (depressive symptoms: <i>B</i> = 0.38, <i>p</i> < 0.001; psychological well-being: <i>B</i> = -0.15, <i>p</i> = 0.046; self-rated health: <i>B</i> = -0.82, <i>p</i> < 0.001; metabolic risk factors: <i>B</i> = 1.49, <i>p</i> < 0.001). Among individual behaviours, the temporal risk score of alcohol consumption was negatively associated with most outcomes, while smoking was associated with poorer mental well-being and physical inactivity with poorer health.</p><p><strong>Conclusions: </strong>The current and temporal accumulation of multiple risky health behaviours were associated with poorer mental well-being and health. Preventing these behaviours early in adulthood and midlife is crucial to avoid their accumulation and subsequent health risks.</p>","PeriodicalId":93874,"journal":{"name":"Annals of medicine","volume":"57 1","pages":"2479233"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12024514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kaili sour soup in alleviation of hepatic steatosis in rats via lycopene route: an experimental study.","authors":"Yi Li, Shuo Cong, Rui Chen, Juan Tang, Liqiong Zhai, Yongmei Liu","doi":"10.1080/07853890.2025.2479585","DOIUrl":"https://doi.org/10.1080/07853890.2025.2479585","url":null,"abstract":"<p><strong>Background: </strong>Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver diseases, with a range of manifestations, such as hepatic steatosis. Our previous study showed that Kaili Sour Soup (KSS) significantly attenuated hepatic steatosis in rats. This study explored the main components of KSS and the mechanisms by which it exerts its protective effects against NAFLD.</p><p><strong>Methods: </strong>Twenty-four 6-week-old male Sprague-Dowley (SD) rats were randomly assigned to three treatments: feeding a normal standard diet, a high-fat diet, or a high-fat diet plus gavage KSS. The effects of KSS treatment on hepatic lipid accumulation were assessed using biochemical, histological, and molecular experiments. The amounts of KSS ingredients were measured using biochemical assays. Network pharmacology analyses were performed to identify the hub genes of KSS targets and enriched pathways. CCK-8 assay was used to determine the effect of free fatty acids (FFA), lycopene, and estrogen on HepG2 viability. Quantitative Real-Time polymerase chain reaction (qRT-PCR) and Western blot assays were performed to determine the effect of KSS or lycopene on estrogen signaling and expression of lipid metabolism-related molecules. Statistical analyses were performed using GraphPad Prism and SPSS.</p><p><strong>Results: </strong>KSS alleviated fat deposition in rat liver tissue and affected the expression of hepatic lipid synthesis, catabolism, and oxidative molecules. Lycopene was identified as the ingredient with the highest amount in KSS. Network pharmacology analyses showed that the hub genes were enriched in the estrogen signaling pathway. Cellular experiments showed that lycopene increased the expression of Estrogen Receptor α (ERα), Carnitine palmitoyltransferase 1 A (<i>CPT1A</i>), Peroxisome proliferator-activated receptor α (<i>PPARα</i>) (all <i>p</i> < 0.01), and Hormone sensitive lipase (<i>HSL</i>) (<i>p</i> < 0.05), and reduced the expression of lipid metabolism-related factors <i>1c(SREBP-1c</i>) (<i>p</i> < 0.01), Acetyl-CoA carboxylase 1 (<i>ACC</i>) and Lipoprotein lipase (<i>LPL</i>) (all <i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>KSS ameliorated abnormal lipid metabolism in patients with NAFLD. Lycopene was the major component of KSS, and it affected estrogen signaling and the expression of lipid metabolism molecules. In short, both KSS and LYC could change lipid metabolism by lowering lipid accumulation and raising lipolysis.</p>","PeriodicalId":93874,"journal":{"name":"Annals of medicine","volume":"57 1","pages":"2479585"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis in <i>Bupleurum chinense</i> DC.","authors":"Han Wang, Shanqun Hu, Tong Li, Xuejie Qu, Jiaqi Zhang, Baoshun Wang, Yixuan Sun, Rui Cao, Yutong Yan, Ze Song, Xia'nan Zhang, Rong Luo, Yuru Tong, Changli Liu","doi":"10.1080/15592324.2025.2495301","DOIUrl":"https://doi.org/10.1080/15592324.2025.2495301","url":null,"abstract":"<p><p><i>Bupleurum chinense</i> DC. a medicinal plant valued for saikosaponins (SSs) with antipyretic and hepatoprotective properties, faces constrained SS biosynthesis mediated by abscisic acid (ABA) during growth. Basic helix-loop-helix (bHLH) transcription factors (TFs) are hypothesized to participate in ABA signaling cascades, but their mechanistic role in SS regulation remains undefined. In this study, 20 differentially expressed <i>BcbHLH</i> genes were identified by transcriptomic profiling of ABA-induced hairy roots, with four MYC-family candidates (<i>BcbHLH1-BcbHLH4</i>) demonstrating ABA-responsive regulatory potential. ABA exposure (100 or 200 μmol/L, 24-72 h) induced dose-dependent SS reduction, while correlation analyses revealed coordinated expression between <i>BcbHLH1-BcHMGR</i> (<i>r</i> = 0.62) and <i>BcbHLH4-BcBAS</i> (<i>r</i> = 0.78), pinpointing these TFs as critical nodes in SS pathway modulation. Tissue-specific profiling showed predominant <i>BcbHLH</i> expression in stems and young leaves, with nuclear localization confirming their transcriptional regulatory organelles. BcbHLH3/4 exhibited transcriptional activation activity in the MYC_N domain, while molecular docking predicted 11th Arginine in the HLH domain as essential for G-box DNA binding. Collectively, our findings suggest that BcbHLH1-BcbHLH4 may serve as potential switches for fine-tuning ABA responsiveness in SS biosynthesis. Strategic manipulation of <i>BcbHLH</i> activity through genetic engineering approaches such as CRISPR-based editing or overexpression could alleviate ABA-mediated biosynthetic repression. Furthermore, precision engineering of the critical functional domain in BcbHLH could enhance promoter-binding activity to target genes and improve SS biosynthesis efficiency. These findings provide a reference framework for harnessing transcriptional regulators to optimize SS production in <i>Bupleurum chinense</i> DC.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"20 1","pages":"2495301"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}