生物医学工程学杂志 Pub Date : 2024-10-25 DOI: 10.7507/1001-5515.202403016

Ying Guo, Shaojie Li

{"title":"[Small-scale cross-layer fusion network for classification of diabetic retinopathy].","authors":"Ying Guo, Shaojie Li","doi":"10.7507/1001-5515.202403016","DOIUrl":"10.7507/1001-5515.202403016","url":null,"abstract":"Deep learning-based automatic classification of diabetic retinopathy (DR) helps to enhance the accuracy and efficiency of auxiliary diagnosis. This paper presents an improved residual network model for classifying DR into five different severity levels. First, the convolution in the first layer of the residual network was replaced with three smaller convolutions to reduce the computational load of the network. Second, to address the issue of inaccurate classification due to minimal differences between different severity levels, a mixed attention mechanism was introduced to make the model focus more on the crucial features of the lesions. Finally, to better extract the morphological features of the lesions in DR images, cross-layer fusion convolutions were used instead of the conventional residual structure. To validate the effectiveness of the improved model, it was applied to the Kaggle Blindness Detection competition dataset APTOS2019. The experimental results demonstrated that the proposed model achieved a classification accuracy of 97.75% and a Kappa value of 0.971 7 for the five DR severity levels. Compared to some existing models, this approach shows significant advantages in classification accuracy and performance.","PeriodicalId":39324,"journal":{"name":"生物医学工程学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Comparative analysis of the impact of repetitive transcranial magnetic stimulation and burst transcranial magnetic stimulation at different frequencies on memory function and neuronal excitability of mice]. [不同频率的重复经颅磁刺激和脉冲经颅磁刺激对小鼠记忆功能和神经元兴奋性影响的比较分析]。

生物医学工程学杂志 Pub Date : 2024-10-25 DOI: 10.7507/1001-5515.202312017

Rui Fu, Haijun Zhu, Chong Ding, Guizhi Xu

{"title":"[Comparative analysis of the impact of repetitive transcranial magnetic stimulation and burst transcranial magnetic stimulation at different frequencies on memory function and neuronal excitability of mice].","authors":"Rui Fu, Haijun Zhu, Chong Ding, Guizhi Xu","doi":"10.7507/1001-5515.202312017","DOIUrl":"10.7507/1001-5515.202312017","url":null,"abstract":"Transcranial magnetic stimulation (TMS) as a non-invasive neuroregulatory technique has been applied in the clinical treatment of neurological and psychiatric diseases. However, the stimulation effects and neural regulatory mechanisms of TMS with different frequencies and modes are not yet clear. This article explores the effects of different frequency repetitive transcranial magnetic stimulation (rTMS) and burst transcranial magnetic stimulation (bTMS) on memory function and neuronal excitability in mice from the perspective of neuroelectrophysiology. In this experiment, 42 Kunming mice aged 8 weeks were randomly divided into pseudo stimulation group and stimulation groups. The stimulation group included rTMS stimulation groups with different frequencies (1, 5, 10 Hz), and bTMS stimulation groups with different frequencies (1, 5, 10 Hz). Among them, the stimulation group received continuous stimulation for 14 days. After the stimulation, the mice underwent new object recognition and platform jumping experiment to test their memory ability. Subsequently, brain slice patch clamp experiment was conducted to analyze the excitability of granulosa cells in the dentate gyrus (DG) of mice. The results showed that compared with the pseudo stimulation group, high-frequency (5, 10 Hz) rTMS and bTMS could improve the memory ability and neuronal excitability of mice, while low-frequency (1 Hz) rTMS and bTMS have no significant effect. For the two stimulation modes at the same frequency, their effects on memory function and neuronal excitability of mice have no significant difference. The results of this study suggest that high-frequency TMS can improve memory function in mice by increasing the excitability of hippocampal DG granule neurons. This article provides experimental and theoretical basis for the mechanism research and clinical application of TMS in improving cognitive function.","PeriodicalId":39324,"journal":{"name":"生物医学工程学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research progress of the effects of high-intensity interval training on excess post-exercise oxygen consumption in human]. [高强度间歇训练对人体运动后多余耗氧量影响的研究进展]。

生理学报 Pub Date : 2024-10-25

Yang-Yang Su, Xiao-Ning Dong, Xiu-Qin Wu

{"title":"[Research progress of the effects of high-intensity interval training on excess post-exercise oxygen consumption in human].","authors":"Yang-Yang Su, Xiao-Ning Dong, Xiu-Qin Wu","doi":"","DOIUrl":"","url":null,"abstract":"Elevated human metabolism during recovery is associated with increased excess post-exercise oxygen consumption (EPOC). EPOC is linearly related to exercise duration and exponentially related to exercise intensity. It is commonly believed that near-maximal intensity interval training prompts the body to produce greater EPOC. This review focuses on the origin and development of high-intensity interval training (HIIT), analyzes its concept, classification and function, and discusses its effects on human EPOC. HIIT promotes a significant increase in EPOC during the fast recovery period, whereas the changes of EPOC during the slow recovery period are still inconclusive; Sprint interval training (SIT) promotes a significant increase in EPOC throughout the whole recovery period. Compared with HIIT, the body's energy expenditure and oxygen uptake (VO2) increase significantly during moderate-intensity continuous training (MICT), but the total energy expenditure and VO2 during exercise and 24 h of recovery period are similar between the two types of exercises, indicating that greater EPOC is generated during the recovery period of HIIT. The mechanisms by which interval training improves EPOC include increasing lung ventilation and catecholamine secretion, accelerating systemic circulation, increasing body temperature, promoting glycogen resynthesis, rapid recruitment of fast twitch muscle fibers and uncoupling of mitochondrial respiration, up-regulating hypoxia inducible factor-1 alpha and skeletal muscle protein, as well as improving intestinal flora.","PeriodicalId":7134,"journal":{"name":"生理学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of voltage-gated potassium channel α subunits in cardiovascular system. 电压门控钾通道 α 亚基在心血管系统中的作用

生理学报 Pub Date : 2024-10-25

Jin-Ru Yang, Peng Huang, Shu-Kuan Ling

引用次数: 0

[Exogenous EPO protects HT22 cells from intermittent hypoxia-induced injury by activating JAK2-STAT5 signaling pathway]. [外源性 EPO 通过激活 JAK2-STAT5 信号通路保护 HT22 细胞免受间歇性缺氧诱导的损伤】。］

生理学报 Pub Date : 2024-10-25

Ke-Rong Qi, Xue Chen, Jian-Chao Si, Qing-Qing Liu, Sheng-Chang Yang

{"title":"[Exogenous EPO protects HT22 cells from intermittent hypoxia-induced injury by activating JAK2-STAT5 signaling pathway].","authors":"Ke-Rong Qi, Xue Chen, Jian-Chao Si, Qing-Qing Liu, Sheng-Chang Yang","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this study was to investigate the effects of exogenous erythropoietin (EPO) on intermittent hypoxia (IH)-induced neuronal injury and the underlying mechanism. Mouse hippocampal neuron HT22 cells were exposed to IH for different durations (1% O2 for 7 min/21% O2 for 3 min, one cycle for 10 min). Cell viability was detected by CCK-8. EPO content in the supernatant of cell culture medium was detected by ELISA kit, and the protein expression was detected by Western blot. EPO receptor (EPOR) protein expression was detected by immunofluorescence staining and Western blot. Cellular apoptosis and mitochondrial membrane potential were detected by the corresponding kits. Reactive oxygen species (ROS) level was detected by DCFH probe, and expression levels of JAK2-STAT5 signaling pathway-related proteins were detected by Western blot. The results showed that IH exposure significantly decreased HT22 cell activity. EPO and EPOR protein expressions were significantly up-regulated at 12 h of IH exposure, but down-regulated at 24 and 48 h. In IH-treated HT22 cells, exogenous EPO significantly increased cell activity and mitochondrial membrane potential, decreased ROS levels and cell apoptosis, up-regulated Nrf-2 and heme oxygenase 1 (HO-1) protein expression levels, decreased Cleaved-Caspase-3/Caspase-3 and Bax/Bcl-2 ratios, and promoted the phosphorylation of JAK2-STAT5 pathway-related proteins. Whereas JAK2 and STAT5 blockers both reversed these neuronal protective effects of EPO. These results suggest exogenous EPO inhibits IH-induced oxidative stress and apoptosis by activating the JAK2-STAT5 signaling pathway, thus exerting a neuronal protective effect.","PeriodicalId":7134,"journal":{"name":"生理学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research progress on the mechanism of autophagy flow injury caused by lysosomal dysfunction after cerebral ischemia]. [脑缺血后溶酶体功能障碍导致自噬流损伤机制的研究进展]。

生理学报 Pub Date : 2024-10-25

Jia-Qian Wang, Hong-Yun He, Yi-Hao Deng

{"title":"[Research progress on the mechanism of autophagy flow injury caused by lysosomal dysfunction after cerebral ischemia].","authors":"Jia-Qian Wang, Hong-Yun He, Yi-Hao Deng","doi":"","DOIUrl":"","url":null,"abstract":"Ischemic stroke is an acute cerebrovascular disease caused by cerebral vascular obstruction, which is the third leading cause of human death and disability. Multiple studies have demonstrated that autophagy plays a positive role in neurons after ischemic stroke. Autophagy is the main intracellular mechanism that mediates the degradation and recycling of various substrates in lysosomes, so it is very important to maintain normal function of lysosomes. However, cerebral ischemia can result in significant impairment of lysosomal function, subsequently leading to disruption in autophagy flow and exacerbation of neuronal injury. This review elucidates the mechanism of autophagic flux injury resulting from lysosomal dysfunction induced by impaired fusion between autophagosomes and lysosomes, alterations in the acidic environment within lysosomes, and diminished biosynthesis of lysosomes following ischemic stroke. The lysosome is regarded as the primary focal point for investigating the mechanism of autophagic flux injury, with the aim of modulating neuronal autophagic flux to improve cerebral ischemia-induced brain injury. This approach holds potential for exerting a neuroprotective effect and providing a novel avenue for stroke treatment.","PeriodicalId":7134,"journal":{"name":"生理学报","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The role of the tryptophan-kynurenine pathway in neuropathic pain]. [色氨酸-犬尿氨酸途径在神经性疼痛中的作用]。

生理学报 Pub Date : 2024-10-25

Zi-Han Wu, Hao-Jun You, Jing Lei

引用次数: 0

[A lightweight convolutional neural network for myositis classification from muscle ultrasound images]. [用于肌肉超声图像肌炎分类的轻量级卷积神经网络]。

生物医学工程学杂志 Pub Date : 2024-10-25 DOI: 10.7507/1001-5515.202301023

Hao Tan, Xun Lang, Tao Wang, Bingbing He, Zhiyao Li, Yu Lu, Yufeng Zhang

{"title":"[A lightweight convolutional neural network for myositis classification from muscle ultrasound images].","authors":"Hao Tan, Xun Lang, Tao Wang, Bingbing He, Zhiyao Li, Yu Lu, Yufeng Zhang","doi":"10.7507/1001-5515.202301023","DOIUrl":"10.7507/1001-5515.202301023","url":null,"abstract":"Existing classification methods for myositis ultrasound images have problems of poor classification performance or high computational cost. Motivated by this difficulty, a lightweight neural network based on a soft threshold attention mechanism is proposed to cater for a better IIMs classification. The proposed network was constructed by alternately using depthwise separable convolution (DSC) and conventional convolution (CConv). Moreover, a soft threshold attention mechanism was leveraged to enhance the extraction capabilities of key features. Compared with the current dual-branch feature fusion myositis classification network with the highest classification accuracy, the classification accuracy of the network proposed in this paper increased by 5.9%, reaching 96.1%, and its computational complexity was only 0.25% of the existing method. The obtained results support that the proposed method can provide physicians with more accurate classification results at a lower computational cost, thereby greatly assisting them in their clinical diagnosis.","PeriodicalId":39324,"journal":{"name":"生物医学工程学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Feature detection of B-ultrasound images of intussusception in children based on improved YOLOv8n]. [基于改进型 YOLOv8n 的儿童肠套叠 B 超图像特征检测]。

生物医学工程学杂志 Pub Date : 2024-10-25 DOI: 10.7507/1001-5515.202401017

Chenyu Liu, Jian Xu, Ke Li, Lu Wang

{"title":"[Feature detection of B-ultrasound images of intussusception in children based on improved YOLOv8n].","authors":"Chenyu Liu, Jian Xu, Ke Li, Lu Wang","doi":"10.7507/1001-5515.202401017","DOIUrl":"10.7507/1001-5515.202401017","url":null,"abstract":"To assist grassroots sonographers in accurately and rapidly detecting intussusception lesions from children's abdominal ultrasound images, this paper proposes an improved YOLOv8n children's intussusception detection algorithm, called EMC-YOLOv8n. Firstly, the EfficientViT network with a cascaded group attention module was used as the backbone network to enhance the speed of target detection. Secondly, the improved C2fMBC module was used to replace the C2f module in the neck network to reduce network complexity, and the coordinate attention (CA) module was introduced after each C2fMBC module to enhance attention to positional information. Finally, experiments were conducted on the self-built dataset of intussusception in children. The results showed that the recall rate, average detection accuracy (mAP@0.5) and precision of the EMC-YOLOv8n algorithm improved by 3.9%, 2.1% and 0.9%, respectively, compared to the baseline algorithm. Despite slightly increased network parameters and computational load, significant improvements in detection accuracy enable efficient completion of detection tasks, demonstrating substantial economic and social value.","PeriodicalId":39324,"journal":{"name":"生物医学工程学杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Levels and risk assessment of dl-PCBs and dioxins in soils surrounded by cement plants from industrial areas of Colombia and Spain 哥伦比亚和西班牙工业区水泥厂周围土壤中 dl-PCB 和二恶英的含量和风险评估

IF 5.3 2区环境科学与生态学

Emerging Contaminants Pub Date : 2024-10-24 DOI: 10.1016/j.emcon.2024.100427

Iñaki Lacomba , Jenny Palomares-Bolaños , Ana Juan-García , Antonio López , Jesús Olivero-Verbel , Karina Caballero-Gallardo , Clara Coscollà , Cristina Juan

{"title":"Levels and risk assessment of dl-PCBs and dioxins in soils surrounded by cement plants from industrial areas of Colombia and Spain","authors":"Iñaki Lacomba , Jenny Palomares-Bolaños , Ana Juan-García , Antonio López , Jesús Olivero-Verbel , Karina Caballero-Gallardo , Clara Coscollà , Cristina Juan","doi":"10.1016/j.emcon.2024.100427","DOIUrl":"10.1016/j.emcon.2024.100427","url":null,"abstract":"<div><div>This study investigates the concentrations of 17 PCDD/Fs and 12 dl-PCBs congeners in soil samples collected from industrial areas in Cartagena de Indias (Colombia) and Valencia (Spain). The aim is to assess the characteristic distribution patterns and the potential risk around cement plants within the selected locations, addressing the lack of data on PCDD/Fs and dl-PCBs in soils from the two studied areas. Soil samples were analysed using gas chromatography coupled to high-resolution mass spectrometry (GC-HRMS). The PCDD/Fs concentrations varied from 0.01 pg g−1 dw to 520.43 pg g−1 dw in Colombia and from 0.01 pg g−1 dw to 150.48 pg g−1 dw in Spain. For dl-PCBs, levels ranged from 0.03 pg g−1 dw to 1611.83 pg g−1 dw in Colombia and from 0.06 to 189.64 pg g−1 dw in Spain. Despite the differences observed in concentration terms between the two areas studied, the same pattern of congeners was observed. The hazard index (HI) values for exposure of adults and children in soil were, in overall, smaller than one (HI < 1), while the total cancer risk (TCR) values exceeded the acceptable risk value of 10−6, which indicate probable non-carcinogenic and carcinogenic risks resulting from exposure to PCDD/Fs and dl-PCBs in these areas. The ecological risk was assessed using the Contamination Factor (CF) and the Ecological Risk Index (ERI), revealing significant contamination in the studied areas.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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