农学最新文献

{"title":"Improving crop image recognition performance using pseudolabels","authors":"Pengfei Deng,&nbsp;Zhaohui Jiang,&nbsp;Huimin Ma,&nbsp;Yuan Rao,&nbsp;Wu Zhang","doi":"10.1016/j.inpa.2024.02.001","DOIUrl":"10.1016/j.inpa.2024.02.001","url":null,"abstract":"<div><div>In crop image recognition, when faced with a large quantity of unlabeled data, the traditional manual labeling method requires a large amount of human and material resources. To solve this problem, this study proposes an image recognition method based on a pseudolabeling technique. First, the data are divided into labeled and unlabeled data. The initial network model is trained on labeled data. Then, pseudolabeling of the unlabeled data is predicted, and only the data that satisfy the confidence threshold are regarded as valid pseudolabeling. To convert the unlabeled data into supervised training data, the two types of data are mixed. The training is terminated when the number of remaining unlabeled data satisfies the end condition and when the fivefold cross-validation method is used to evaluate model performance. Compared with the traditional semisupervised method, the experimental method is simpler and more applicable. Experiments were conducted on rice growth stage recognition and crop weed seedling recognition tasks. The results showed that the proposed method achieved 99.17% accuracy in rice growth stage recognition and a high AUC value of 99.93% in crop weed seedling recognition, which demonstrated excellent performance. Compared with the traditional model, this method not only improves in accuracy but also has better stability and wider applicability and is expected to provide an efficient, accurate and scalable solution for crop image recognition.</div></div>","PeriodicalId":53443,"journal":{"name":"Information Processing in Agriculture","volume":"12 1","pages":"Pages 17-26"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Security analysis of agricultural energy internet considering electricity load control for dragon fruit cultivation","authors":"Xueqian Fu ,&nbsp;Lingxi Ma ,&nbsp;Huaichang Ge ,&nbsp;Jiahui Zhang","doi":"10.1016/j.inpa.2024.02.002","DOIUrl":"10.1016/j.inpa.2024.02.002","url":null,"abstract":"<div><div>With the increasing emphasis on sustainable energy and the advancements in modern agriculture, flexible agricultural power loads present challenges to the reliable operation of the agricultural energy internet. However, research on the coupling of energy system security with agricultural security is insufficient and fails to consider the impacts of meteorological elements on agricultural power loads. To address these gaps, this paper establishes load models for irrigation and light supplementation based on the actual cultivation demands of winter dragon fruit in Guangxi province. A static security index system is developed to analyze the security, considering the unique features of agricultural power demands. The condition of the distribution network is assessed by comparing the indexes with predefined limits, using a China 41-bus distribution network. Finally, the optimal scheme for nocturnal supplemental lighting treatment and irrigation is determined based on the method for maintaining secure operation of the distribution network. This study serves as a guide for simulating current farming power loads and demonstrates how security analysis of the agricultural energy internet contributes to the large-scale and sophisticated development of modern agriculture.</div></div>","PeriodicalId":53443,"journal":{"name":"Information Processing in Agriculture","volume":"12 1","pages":"Pages 27-39"},"PeriodicalIF":7.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics and diversity of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in Vietnam","authors":"Tuan-Binh Huynh ,&nbsp;Thuy-Dung Mai-Hoang ,&nbsp;Tram-Anh Bui-Nguyen ,&nbsp;Trinh Thi Phuong Ha ,&nbsp;Minh-Duy Phan ,&nbsp;Hieu Tran-Van","doi":"10.1016/j.aaf.2023.10.004","DOIUrl":"10.1016/j.aaf.2023.10.004","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) is a contagious disease in shrimp with extremely high mortality rate, causing an estimated $23.6 billion loss to the global shrimp industry during 2009–2016. A binary toxin, PirAB, is the causative agent, which is encoded by the <em>pirAB</em>^<em>vp</em> genes on a virulent plasmid typically found in <em>Vibrio parahaemolyticus</em>. Here, seventeen strains of <em>V. parahaemolyticus</em> were isolated from the Mekong Delta of Vietnam, of which 15 harboured identical <em>pirAB</em>^<em>vp</em> genes. The remaining two strains, which were PCR-negative for <em>pirAB</em>, and nine positive strains were selected for whole genome sequencing. Multi-locus sequence typing revealed five sequence types (STs), including one novel, that were associated with sites of isolation. A maximum likelihood tree of core genomes revealed that our strains formed three clusters and were closely related to strains from Thailand, South Korea, China, and Philippines. All sequenced strains carried β-lactam (including <em>bla</em>_OXA-10 and <em>bla</em>_NDM-1) and tetracycline resistance genes, with a range of 3–13 resistance genes. The <em>pirAB</em>-positive strains shared large regions of homology with the prototypical AHPND virulent plasmid. In summary, this work revealed the characteristics of AHPND-causing strains in the Mekong Delta of Vietnam and suggested possible transmission routes among Vietnam and other shrimp farming countries.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 2","pages":"Pages 202-209"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139294398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does soak time influence the effect of artificial light on catch efficiency in snow crab (Chionoecetes opilio) pot fishery?","authors":"Kristine Cerbule ,&nbsp;Bent Herrmann ,&nbsp;Eduardo Grimaldo ,&nbsp;Leif Grimsmo ,&nbsp;Junita D. Karlsen","doi":"10.1016/j.aaf.2023.11.004","DOIUrl":"10.1016/j.aaf.2023.11.004","url":null,"abstract":"<div><div>In the Barents Sea commercial snow crab (<em>Chionoecetes opilio</em>) fishery, an increase in catch efficiency of the conical pots is important for the profitability of the industry. Light emitting diodes (LEDs) have previously been tested for increasing catch efficiency of the snow crab pots. These earlier experiments have shown varying results ranging from large increase in snow crab catches to no significant effect. These experiments have used different pot soaking times; however, the soaking time might affect the impact of LEDs on catch efficiency. In commercial snow crab fishery, the pot soak time is varying which has not been considered in earlier experiments testing the effect of LEDs. Therefore, this study examined whether pot soaking time can explain the observed differences in relative catch efficiency of snow crab pots with and without LEDs with soak times ranging from 2 to 14 days in the Barents Sea snow crab fishery. For target sizes of snow crab (≥95 mm carapace width), results indicated an increase in catch efficiency between 10 and 30% for pots with LEDs with exception of one experiment using six days soak time. However, experimental results were subjected to large uncertainties and, except from one experiment with five days soak time, the estimated increases were nonsignificant. Furthermore, the pot soak time was not found to impact the effect of white LEDs on capture efficiency.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 2","pages":"Pages 330-343"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of pulp and viscera from hybrid sorubim and pacu by production of protein concentrate using alkaline solubilization","authors":"Suéllen Patrícia Held Azambuja ,&nbsp;Kauyse Matos Nascimento ,&nbsp;William Renzo Cortez-Vega ,&nbsp;Angela Dulce Cavenaghi-Altemio ,&nbsp;Jane Mary Lafayette Neves Gelinski ,&nbsp;Gustavo Graciano Fonseca","doi":"10.1016/j.aaf.2023.08.012","DOIUrl":"10.1016/j.aaf.2023.08.012","url":null,"abstract":"<div><div>Fish protein concentration (FPC) by alkaline solubilization has proven to be an alternative method in adding value to muscle-based materials. The aim of this work was to evaluate the influence of different processes (decanting/sieving or centrifugation), reagents (sodium bicarbonate or sodium hydroxide) and washing cycles (1–4) on the quality of protein concentrates obtained from mechanically separated meat (MSM) and viscera of hybrid sorubim (<em>Pseudoplatystoma reticulatum</em> × <em>P. corruscans</em>) and pacu (<em>Piaractus mesopotamicus</em>) fishes. The protein concentrate was obtained by washing the viscera or MSM during up to four cycles (4:1 solution:meat ratio). In each cycle, the liquid waste and fat were removed by decanting/sieving or centrifugation. Moisture, crude protein, crude fat and crude ash contents were determined after each washing cycle. The moisture and protein contents increased immediately after the first washing up to 77.88% (dry basis). The lipid content decreased with the cycles down to 2.99% (dry basis). Higher FPC yield was obtained for MSM during the centrifugation process compared to the decantation/sieving process. Two weight yields above 100% were found in procedures with NaHCO₃ and MSM of hybrid sorubim and pacu, probably due to the larger capacity of gel formation during protein concentration. Differences related to the fish species were more evident than the processes and utilized reagents.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 2","pages":"Pages 312-320"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139292527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plankton community in Penaeus vannamei nurseries with synbiotic system under different frequencies of adjustment on Ca:Mg:K ratio in low salinity water","authors":"Otávio Augusto Lacerda Ferreira Pimentel ,&nbsp;Valdemir Queiroz de Oliveira ,&nbsp;Rildo José Vasconcelos de Andrade ,&nbsp;Carlos Yure Barbosa de Oliveira ,&nbsp;Elizabeth Pereira dos Santos ,&nbsp;Alitiene Moura Lemos Pereira ,&nbsp;Luis Otavio Brito ,&nbsp;Alfredo Olivera Gálvez","doi":"10.1016/j.aaf.2023.09.003","DOIUrl":"10.1016/j.aaf.2023.09.003","url":null,"abstract":"<div><div>The aim of this study was to evaluate plankton communities in <em>Penaeus vannamei</em> nurseries under different frequencies of adjustment on Ca:Mg:K ratio in low salinity water and with synbiotic system. A nursery culture was carried out for 40 days, using 2000 shrimp m⁻³. Following treatments were established: 1IA (Ca:Mg:K ratio adjustment to 1:3:1 on day 1); 2IA (Ca:Mg:K ratio adjustment to 1:3:1 on day 1 and day 20); 3IA (Ca:Mg:K ratio adjustment to 1:3:1 on day 1, day 10, and day 20); SW (seawater - salinity of 31 g L⁻¹); and SWD (seawater diluted to a salinity of 2.3 g L⁻¹). Rice bran processed by probiotic microorganisms was used as an organic carbon source. Treatments did not have a significant effect on phytoplankton and zooplankton composition. However, plankton composition was significantly different over the experimental course. A reduced abundance of Cyanophyta was observed compared to traditional aquaculture systems. This can be explained by the probiotic bacteria addition to the system, along with fertilization, which may have limited the growth of this harmful algae. Throughout the experiment, zooplankton community had a dominance by protozoan microorganisms, such as amoebae and ciliates. The presence of copepods and rotifers was also observed throughout the experimental course. This proves that the use of the synbiotic system provides conditions for the microbial loop development, mimicking a natural environment. Different adjustment frequencies in the water Ca:Mg:K ratio do not significantly change plankton composition in <em>P. vannamei</em> intensive nurseries using low salinity water and synbiotic system. Temporal variation in plankton composition probably occurred due to fertilization of the system with an organic carbon source. The use of the synbiotic system proved to be efficient in microbial loop development, providing the growth of a high load of microorganisms that can influence water quality and shrimp growth in low salinity water.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 2","pages":"Pages 289-297"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135848626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: The histone H3K27 demethylase SlJMJ4 promotes dark- and ABA-induced leaf senescence in tomato.","authors":"","doi":"10.1093/hr/uhaf046","DOIUrl":"https://doi.org/10.1093/hr/uhaf046","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1093/hr/uhab077.].</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"12 2","pages":"uhaf046"},"PeriodicalIF":7.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11878786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: DgbZIP3 interacts with DgbZIP2 to increase the expression of <i>DgPOD</i> for cold stress tolerance in chrysanthemum.","authors":"","doi":"10.1093/hr/uhaf047","DOIUrl":"https://doi.org/10.1093/hr/uhaf047","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1093/hr/uhac105.].</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"12 2","pages":"uhaf047"},"PeriodicalIF":7.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11848036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143494855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus atrophaeus DX-9 biocontrol against potato common scab involves significant changes in the soil microbiome and metabolome","authors":"Jingjing Cao,&nbsp;Yue Ma,&nbsp;Jing Fu,&nbsp;Zhiqin Wang,&nbsp;Yonglong Zhao,&nbsp;Naiqin Zhong,&nbsp;Pan Zhao","doi":"10.1007/s42994-025-00199-3","DOIUrl":"10.1007/s42994-025-00199-3","url":null,"abstract":"<div><p>Potato common scab (CS) is a worldwide disease, caused by <i>Streptomyces</i> spp., and its presence reduces the market value of potatoes. A nontoxic and potentially effective approach in many control strategies is the use of antagonistic microbes as biocontrol agents. In this study, <i>Bacillus atrophaeus</i> DX­9 was isolated and assessed for its ability to protect against CS. Through integrated metagenomic and metabolomic analyses, changes in the soil microbial community structure and soil properties were analyzed to understand the effects of <i>Bacillus atrophaeus</i> DX­9 on CS. These studies revealed that DX­9 inoculation could significantly decrease CS disease rate, disease index, and the number of CS pathogens, along with an increase in soil N and P content. Our metagenomic assays identified 102 phyla and 1154 genera, and DX­9 inoculation increased the relative abundances of the phyla Pseudomonadota, Chloroflexota and Gemmatimonadota. Additionally, an increase in the relative abundance of genera, such as <i>Bradyrhizobium</i>, <i>Agrobacterium</i>, and <i>Nitrobacter</i>, were significantly and positively correlated with soil N and P. Metabolomic analysis revealed that DX­9 inoculation significantly increased the soil levels of phytolaccoside A, 7,8­dihydropteroic acid, novobiocin, and azafrin. These compounds were enriched in microbe pathway metabolites, including xenobiotic biodegradation and metabolism, biosynthesis of other secondary metabolites, and metabolism of cofactors and vitamins. In summary, the use of <i>Bacillus atrophaeus</i> DX­9 against potato CS offers an alternative biocontrol method that can improve both soil microbial community and properties. This study provides insight into the potential mechanisms by which microbial inoculants can control CS disease.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 1","pages":"33 - 49"},"PeriodicalIF":4.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-025-00199-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenous U6 promoters and visual reporters","authors":"Xiuzhi Xia,&nbsp;Shihao Li,&nbsp;Na Wang,&nbsp;Panxu Cheng,&nbsp;Butuo Zhu,&nbsp;Pengcheng Zhang,&nbsp;Dahai Yang,&nbsp;Hao Lin,&nbsp;Lifang Niu","doi":"10.1007/s42994-025-00200-z","DOIUrl":"10.1007/s42994-025-00200-z","url":null,"abstract":"<div><p>CRISPR/Cas is a simple, robust, versatile tool for plant biology studies and precision plant breeding. However, establishing a high-efficiency gene editing system for multiplex editing of the autotetraploid crop alfalfa (<i>Medicago sativa</i> L.), the most important forage legume worldwide, remains a formidable challenge. Here, we systematically identified endogenous <i>U6</i> promoters in alfalfa through transient expression via <i>Agrobacterium</i>-mediated infiltration of alfalfa leaves. We further demonstrated the efficacy of the three most active promoters for genome editing using an optimized alfalfa hairy root system. Subsequently, we established an improved CRISPR/Cas9 multiplex system containing three or four tandemly arrayed <i>MsU6</i>-promoter-driven polycistronic tRNA-sgRNA (PTG) expression cassettes, each consisting of three tRNA-sgRNA units, to simultaneously edit three or four alfalfa genes, coupled with the visual reporter <i>RH1</i> or <i>RUBY</i>. This toolkit showed efficient multiplex editing in the hairy root system with visual selection. We successfully obtained regenerated, red-colored shoots resulting from the stable transformation of alfalfa. These results highlight the potential application of the visual reporter system for the stable transformation of alfalfa. Our improved CRISPR/Cas9 multiplex system enables convenient, high-efficiency multiplex genome editing in alfalfa, providing a versatile toolset to facilitate functional studies of multiple genes and gene families for basic research and the genetic improvement of alfalfa.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 1","pages":"81 - 90"},"PeriodicalIF":4.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-025-00200-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}