Isolation and purification of antioxidant peptides from swim bladder of grass carp (Ctenopharyngodon idella)

Q1 Agricultural and Biological Sciences

Aquaculture and Fisheries Pub Date : 2025-05-01 DOI:10.1016/j.aaf.2023.12.009

Suxin Li , Jinhui Gu , Biluan Zhong , Rui Feng , Hao Pan , Yiyi Liu , Wenzheng Shi

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Abstract

Swim bladder is a kind of material with rich nutritional value, wide access and a good source of collagen. In order to make it more fully utilized and absorbed by human body, using grass carp swim bladder as raw material and alkaline protease-neutral protease to prepare collagen peptide, the degree of hydrolysis can reach 36.91%. The results proved that after purification by ultrafiltration membrane and gel column chromatography, the antioxidant activity of GCP generally increased with the increase of concentration, and the smaller the molecular weight of GCP obtained after separation and purification, the stronger the overall antioxidant activity. Among them, the ABTS⁺ scavenging rate of GCP with a certain molecular weight was 88.64%. Moreover, GCP also has good SOD scavenging ability and ferrous ion chelating ability. The total antioxidant activity of GCP-II purified by G15 gel column is great, for it exerts considerable antioxidant capacity at a lower concentration.

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从草鱼鳔中分离和纯化抗氧化肽

鱼鳔是一种营养价值丰富、获取途径广泛的物质，是胶原蛋白的良好来源。为使其更充分地被人体利用和吸收，以草鱼鱼鳔为原料，采用碱性蛋白酶-中性蛋白酶制备胶原肽，水解度可达36.91%。结果证明，经超滤膜和凝胶柱层析纯化后，GCP的抗氧化活性普遍随浓度的增加而增加，分离纯化后得到的GCP分子量越小，整体抗氧化活性越强。其中，具有一定分子量的GCP的ABTS+清除率为88.64%。此外，GCP还具有良好的SOD清除能力和铁离子螯合能力。G15凝胶柱纯化的GCP-II总抗氧化活性高，在较低浓度下也具有相当的抗氧化能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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