{"title":"Evaluation of growth performances of crossbreds and backcrossbreds with purebreds Nishikigoi and Hungarian carp (Cyprinus carpio)","authors":"Vander Bruno Santos, Lucas Rosan Furquim","doi":"10.1016/j.aaf.2023.11.003","DOIUrl":"10.1016/j.aaf.2023.11.003","url":null,"abstract":"<div><div>This study aimed to evaluate growth curves among different common carp genotypes, according to fingerling performance and morphometry. Fingerlings selected according to Nishikigoi pattern, either (9.66 ± 1.47) cm in standard length, from crossbred and pure carp, respectively, were cultivated in 8 cages (4 replicates for each genotype), measuring 9 m³ (3.0 m × 2.0 m × 1.5 m) each, in a 1500 m<sup>2</sup> (12 m × 125 m) pond at a depth of 1.60 m with 10 fingerlings/m³. Fish were fed four times a day at the rate of 5%–7% of biomass with the same specific diet for each growth stage. Forty carp of each genotype (10 fish/cage) were weighed at days 0, 60, 150, 270 and 330 of cultivation. They were weighed and measured for head length, standard length, body height and body width. The Gompertz model was fit for weight × age data for comparison of absolute and relative growth rate, weight and age at the inflection points. The crossbred genotype showed better survival, weight gain and feed conversion compared to pure carp (<em>P</em> < 0.05). The final weight estimate at 330 days was 38.45% higher in the crossbred genotype (289.16 g) when compared to the pure carp (208.85 g). The pure genotype presented lower weight (431.60 g), age (559.71 days) and absolute growth rate (1.03 g/day) at the inflection points when compared to the crossbred genotype (587.69 g; 559.71 days and 1.46 g/day, respectively). Body morphometric relationships changed with fish growth, and this occurred differently, depending on carp genotype evaluated. From these results, it can be concluded that crossing Nishikigoi with Hungarian carp can be a strategy to improve desirable characteristics in koi.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 452-458"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139538253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role and mechanism of UFMylation, a ubiquitin-like modification, in <i>Mycobacterium tuberculosis</i> infection immunity.","authors":"Yi-Ran Liao, Qi-Ao Zhang, Pei-Bo Li, Jian-Ping Xie","doi":"10.16288/j.yczz.24-259","DOIUrl":"https://doi.org/10.16288/j.yczz.24-259","url":null,"abstract":"<p><p>Ubiquitin-fold modifier 1 (FM1) is a ubiquitin-like type I protein widely present in prokaryote and most eukaryote. UFMylaiton, mediated by UFM1, is involved in the regulation of a variety of cellular biochemical processes. Recently, the importance of UFM1 system in endoplasmic reticulum homeostasis regulation has been gradually discovered and emphasized. Endoplasmic reticulum stress caused by <i>Mycobacterium tuberculosis</i> is an important link in the progression of tuberculosis, so UFM1 system is expected to become a new target for the development of anti-tuberculosis drugs. In this review, we provide a brief overview of the UFM1 system and UFMylation pathway, and summarize the recent advances in understanding UFM1's role in ER homeostasis regulation and its potential value in TB treatment, with the aim of offering new insight and direction for developing novel therapeutic strategies against tuberculosis.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"546-557"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-05-01DOI: 10.16288/j.yczz.24-368
Fan Bai, Wei He, Yu Gao, Wan-Jing Ping, Qiao-Mei Fu
{"title":"Ancient genomes revealed the genetic history and interaction of prehistoric populations in the Ngari region of the western Tibetan Plateau.","authors":"Fan Bai, Wei He, Yu Gao, Wan-Jing Ping, Qiao-Mei Fu","doi":"10.16288/j.yczz.24-368","DOIUrl":"https://doi.org/10.16288/j.yczz.24-368","url":null,"abstract":"<p><p>The Ngari Prefecture is located in the western part of the Tibetan Plateau. Despite its high altitude and harsh natural environment, it occupies a strategic position adjacent to northern Nepal, India, and Ladakh. As a crossroads connecting the Tibetan Plateau, Xinjiang Autonomous Region, Central Asia, and South Asia, it likely played an important role in early human migration. Archaeological studies in recent years have revealed that even before the establishment of the Tubo Dynasty in the seventh century, the Ngari region maintained extensive cultural connections with Kashmir, Nepal, Xinjiang, and other regions. Furthermore, its subsistence economy reflected strong interactions between Ngari and northern South Asia. Recent paleogenetic studies have demonstrated that the genetic components of the ancient population in the Ngari region originated from the southern part of the plateau, with multiple population expansions occurring from south to west. These studies have also confirmed that alongside cultural exchanges, the Ngari region began experiencing complex genetic interactions with ancient populations from neighboring Central and South Asia at least 2,300 years ago. In this review, we integrate findings from paleogenetics, archaeology, archaeobotany, and zooarchaeology in the Ngari region to systematically examine the genetic origins of the ancient western plateau population and analyze both intraregional cultural-genetic interactions within the Tibetan Plateau and those between Ngari populations and external groups. This synthesis provides important insights for understanding the complex relationships between ancient population movements and interactions in the western Tibetan Plateau, as well as the transformation patterns of regional archaeological cultures and subsistence economies.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"501-512"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-05-01DOI: 10.16288/j.yczz.24-217
Sai-Nan An, Huan-Chun Yang, Shan Jiang, Jing-Xuan Li, Gen-Fa Zhang
{"title":"Design and exploration of integrating bioinformatic analysis into comprehensive and exploratory epigenetic experiments.","authors":"Sai-Nan An, Huan-Chun Yang, Shan Jiang, Jing-Xuan Li, Gen-Fa Zhang","doi":"10.16288/j.yczz.24-217","DOIUrl":"https://doi.org/10.16288/j.yczz.24-217","url":null,"abstract":"<p><p>As the most thoroughly studied epigenetic modification, DNA methylation-induced silencing of suppressor genes is closely related with the development of cancers. Bioinformatic analysis is an important research tool in the fields of life sciences, medicine, and so on. To introduce the application of bioinformatic analysis in cancer epigenetic research to students and to change the current situation that students usually passively perform experiments during the experimental teaching of genetics, we established a comprehensive and exploratory epigenetic experiment which was integrated with bioinformatic analysis. The implement of this experiment followed the principles of centering on students and cultivating scientific research capabilities. Students selected the genes that were potentially silenced by high DNA methylation in lung adenocarcinoma through bioinformatic analysis and literature review under the guidance of teachers and formulated experimental scheme by their own. They then conducted real-time PCR and methylation-specific PCR to explore whether DNA methylation caused the expression silencing of genes of interest and to identify the DNA methyltransferase responsible for the methylation of genes of interest. To comprehensively assess the capability of students, we designed diversified evaluation methods which intensify the assessment of experiment process. This experiment has the characteristics of interdisciplinarity, comprehensiveness, and inquiry, which can help to cultivate the scientific thinking and practical ability of students.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"600-608"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chocolate squid (Todarodes pacificus) bait reduces snow crab catch rates","authors":"T. Araya-Schmidt, P.D. Winger, G. Peck","doi":"10.1016/j.aaf.2023.12.001","DOIUrl":"10.1016/j.aaf.2023.12.001","url":null,"abstract":"<div><div>This study evaluated the performance of chocolate squid (<em>Todarodes pacificus</em>) bait as a lower-cost alternative to traditional squid bait in a snow crab (<em>Chionoecetes opilio</em>) fishery in Eastern Canada. The results showed that pots with chocolate squid bait had significantly lower catch rates compared to pots with traditional squid bait. The chocolate squid bait significantly reduced the number of crabs caught per pot by 13.99% and the weight of crabs caught per pot by 2.88 kg. Although the chocolate squid bait is cheaper, the decrease in catch rates could require increased effort to reach individual quotas, which could lead to longer or more frequent fishing trips and more traps being fished, increasing fuel consumption and habitat disruption, resulting in negative environmental impacts. Finding alternative lower-cost baits that maintain catch rates remains a key priority for reducing operational costs and minimizing environmental impacts in the snow crab fishery.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 494-498"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histological observations and gene expression of the digestive system of the Chinses mitten crab (Eriocheir sinensis)","authors":"Zhiyong Zhang , Hewei Jiang , Yufei Liu , Xugan Wu , Xiaowu Chen","doi":"10.1016/j.aaf.2023.12.003","DOIUrl":"10.1016/j.aaf.2023.12.003","url":null,"abstract":"<div><div>In order to elucidate the structural characteristics and gene expression of the tissues of the digestive system of adult <em>Eriocheir sinensis</em>, this study was conducted to systematically study the digestive system of <em>E</em>. <em>sinensis</em> using histological and transcriptomic techniques. The results showed that the digestive system of <em>E</em>. <em>sinensis</em> mainly consisted of the esophagus, stomach (including cardia stomach and pyloric stomach), midgut, intestinal bulb, hindgut and hepatopancreas. In the cardia stomach, calcification of the cuticle into cuticular ossicles, chitin tooth, ridge, bristles and other structures were observed at different sites, which together form the \"Gastric mill\" for grinding food. The pyloric stomach contains special comb-like structures and rows of pectinate bristles, which are important components of the gland filter for filtering and sifting food. The wall of the digestive tract of <em>E. sinensis</em> consists of four layers: the mucosal layer, the submucosal layer, the muscular layer and the outer membrane, of which the surface of the mucosal layer of the esophagus and hindgut has a well-developed cuticle with rows of setae, and the submucosal layer has special mucus glands (esophageal glands or hindgut glands); the surface of the mucosal layer of the midgut has no cuticle and has dense microvillus. The intestinal bulb has the morphological characteristics of the transition from midgut to hindgut. The hepatopancreas consists of many hepatic tubules, and four types of cells are often seen in the hepatic tubules, including absorptive cells (R cells), vesicular cells (B cells), fibroblasts (F cells), and embryonic cells (E cells). The transcriptome data of the digestive system of <em>E</em>. <em>sinensis</em> showed that Esophagus, Stomach, Midgut, Intestinal bulb, Hindgut and hepatopancreas expressed 13252, 13285, 13634, 11147, 13127, 12551 genes respectively; and they specifically expressed 1529, 549, 1199, 624,990, 1688 genes respectively. A total of 25 digestive enzyme genes were isolated from six tissues of the digestive system. Except for maltosidase-glucoamylase and phosphatase A2, which had small differences in expression in various tissues of the digestive system, all other digestive enzymes had the highest expression in the hepatopancreas. Digestive enzymes with high expression in the hepatopancreas are trypsin, pancreatic rennin, carboxypeptidase B, ɑ-amylase, and astacin.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 411-420"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Current updates on viral infections affecting tilapia","authors":"Christal Winona Clyde, Jin Ping Tan, Swee Keong Yeap, Chean Yeah Yong","doi":"10.1016/j.aaf.2024.05.002","DOIUrl":"10.1016/j.aaf.2024.05.002","url":null,"abstract":"<div><div>Tilapia aquaculture is a commercially important and lucrative trade with global production predicted to increase in the future. Owing to the robust and disease-resistant nature of tilapia, tilapia aquaculture has expanded and intensified over the years. However, the intensification of tilapia aquaculture has led to the emergence of diseases, whereby viral diseases represent a prominent limiting factor to the growth of aquaculture. This review aims to discuss all viral infections in tilapia, with emphasis on the aetiology, occurrence, transmission, pathology, diagnostic methods, and control measures to mitigate outbreaks of viral diseases in aquaculture. To date, ten viral infectious diseases have been reported globally in various tilapia species reared in aquaculture or laboratory settings. To our knowledge, this review article represents the first instance of reviewing tilapia parvovirus (TiPV) infection in tilapia, in addition to tilapia larvae encephalitis virus (TLEV), infectious spleen and kidney necrosis virus (ISKNV), iridovirus-like agent, novel megalocytivirus, Bohle iridovirus (BIV), lymphocystis disease virus (LCDV), infectious pancreatic necrosis virus (IPNV), nervous necrosis virus (NNV), and tilapia lake virus (TiLV).</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 355-371"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141695085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
遗传Pub Date : 2025-05-01DOI: 10.16288/j.yczz.24-288
Jie-Hao Lin, Tong-Shu Yang, Wen-Qing Zhang, Wei Liu
{"title":"Role of different Lyl1 transcripts in zebrafish primitive hematopoiesis.","authors":"Jie-Hao Lin, Tong-Shu Yang, Wen-Qing Zhang, Wei Liu","doi":"10.16288/j.yczz.24-288","DOIUrl":"https://doi.org/10.16288/j.yczz.24-288","url":null,"abstract":"<p><p>Primitive hematopoiesis is a crucial process in the organism, responsible for the transportation of oxygen and nutrients during early embryonic stages and laying the foundation for the immune system. During primitive hematopoiesis, hematopoietic-related transcription factors and their cofactors interact to form a complex regulatory network that controls the process of primitive hematopoiesis. Among the bHLH transcription factor family, SCL and LYL1 are key factors in embryonic hematopoiesis. SCL is responsible for initiating primitive hematopoiesis, while LYL1, a paralog of SCL, compensates for the hematopoietic impact of SCL deficiency in adulthood. However, the role of LYL1 in primitive hematopoiesis remains unclear. This study, through analysis of zebrafish blood cell scRNA-seq data, discovered high expression of <i>CABZ01066694.1</i> in hematopoietic stem/progenitor cells. Sequence alignment revealed it as a short transcript of the <i>lyl1</i> gene. Subsequently, using 5'RACE and sequencing, the study confirmed the existence of both long (<i>lyl1f</i>) and short (<i>lyl1s</i>) transcripts of <i>lyl1</i> in zebrafish and humans, similar to mice. Further analysis of scRNA-seq and RNA-seq data from public databases showed that in zebrafish primitive hematopoietic cells, <i>lyl1</i> primarily transcribes <i>lyl1s.</i> Finally, using Morpholino technology to knock down <i>lyl1f</i> and <i>lyl1s</i> separately, it was found that knocking down <i>lyl1s</i> hindered the production of primitive myeloid progenitors and primitive granulocytes, whereas knocking down <i>lyl1f</i> promoted the production of primitive macrophages. In conclusion, this study demonstrates the existence of long and short transcripts of <i>lyl1</i> in zebrafish and humans, with distinct roles in regulating primitive myelopoiesis, providing new insights into the regulation of primitive hematopoiesis.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"573-588"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marios Charalambides , Michalis Menicou , George Triantaphyllidis
{"title":"Economic feasibility study for the expansion of the Cyprus aquaculture sector: A roadmap for transition to offshore in the Mediterranean Sea","authors":"Marios Charalambides , Michalis Menicou , George Triantaphyllidis","doi":"10.1016/j.aaf.2023.12.006","DOIUrl":"10.1016/j.aaf.2023.12.006","url":null,"abstract":"<div><div>Global demand for seafood is constantly increasing, driven by population growth and rising per capita consumption. While wild-capture fisheries are facing limitations in meeting this demand, the expansion of aquaculture emerges as the only solution. This paper investigates the potential for increasing the aquaculture production in the Republic of Cyprus by transitioning towards offshore aquaculture farms. The model developed provides a valuable roadmap for offshore transitions in Mediterranean near-coast areas. The study includes a detailed economic analysis coupled with sensitivity analysis to assess the feasibility of this transition considering various expansion scenarios. The results provide valuable insights into the viability and implications of this process, highlighting economic and operational issues.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 522-531"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139872976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christopher B. Rillahan , Michael T. Coute , Daniel Ward , Pingguo He
{"title":"Comparing the growth and survival of tautog (Tautoga onitis) fed a diet of green crab (Carcinus maenas) or commercial pellets in a recirculating aquaculture system","authors":"Christopher B. Rillahan , Michael T. Coute , Daniel Ward , Pingguo He","doi":"10.1016/j.aaf.2024.02.005","DOIUrl":"10.1016/j.aaf.2024.02.005","url":null,"abstract":"<div><div>Tautog, a coastal marine wrasse, is a highly sought food fish in southern New England and the Mid-Atlantic region of the United States due to its firm white fillet. Tautog has been identified as a candidate species for marine aquaculture in the region. Unfortunately, previous grow out studies have documented slow growth, which is detrimental for aquaculture. However, these studies contrast with data from wild populations which indicate rapid growth in the first few years of life. It is theorized that these differences in growth could be due to available diets. In the wild, tautog primarily prey on mollusks and crustacean prey, while laboratory studies rely on commercial fishmeal-based diets. This study compared the growth and survival of juvenile tautog fed with chopped green crabs, commercial fish pellets and a hybrid diet consisting of both green crabs and pellets. Wild juvenile tautog were collected from local waters and cultivated in a recirculating aquaculture system for 250 days. Tautog fed the commercial pellet diet had a growth rate of 0.31 ± 0.11 mm/day, consistent with previous grow out studies. The average growth rate of tautog fed the green crab diet was 0.52 ± 0.13 mm/day, with a peak growth rate of 0.66 ± 0.07 mm/day. The hybrid diet was intermediate to the other treatments at an average growth rate of 0.46 ± 0.1 mm/day. Statistically significant differences were observed across treatment groups (<em>P</em> < 0.05). Survival was high in the green crab and hybrid treatments (96.7% ± 1.2% and 96.0% ± 2.0%, respectively), and lower in the pellet treatment (85.3% ± 9.5 %). Data from this study indicate that the growth potential of tautog is higher than previously documented in aquaculture studies. This study highlights the need for additional research to develop feed formulations for non-piscivorous marine carnivores.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 469-474"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140401064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}