农学最新文献

{"title":"Combined supplementation of hydroxyproline and vitamin C improved the growth and flesh quality of Pacific white shrimp (Litopenaeus vanname) cultured in low salinity water","authors":"Tianyu Huang ,&nbsp;Beibei Guo ,&nbsp;Jinyu Zheng ,&nbsp;Menglu Li ,&nbsp;Yunfeng Chen ,&nbsp;Xiaoqin Li ,&nbsp;Xiangjun Leng","doi":"10.1016/j.aaf.2023.12.007","DOIUrl":"10.1016/j.aaf.2023.12.007","url":null,"abstract":"<div><div>This study investigated the effects of hydroxyproline (Hyp) and vitamin C (VC) addition to practical diet on growth and flesh quality of Pacific white shrimp (<em>Litopenaeus vannamei</em>) cultured in low salinity water. The control diet was formulated to contain 200 g/kg fish meal (FM20), and then 14 g/kg coated hydroxyproline (50%) (FM20H), 2 g/kg L-ascorbyl-2-phosphate (38%) (FM20C) and their combination (FM20HC) were added to the control diet to form four isoproteic (41.6%) and isolipidic (6.7%) diets. Pacific white shrimp with initial body weight of (1.50 ± 0.05) g were fed the above four diets in low salinity water (salinity 1‰) for 8 weeks. The results showed that the combined supplementation of Hyp and VC (FM20HC) increased the weight gain by 13.4%, and decreased the feed conversion ratio by 0.18 (<em>P</em> &lt; 0.05), while the individual supplementation (FM20H and FM20C groups) just numerically promoted the growth (<em>P</em> &gt; 0.05). In addition, the FM20HC group also showed higher protein and lipid retention than the FM20 group (<em>P</em> &lt; 0.05). In flesh quality, the supplementation of Hyp (FM20H) increased total collagen and heat-soluble collagen contents, flesh chewiness and free flavor amino acids contents (<em>P</em> &lt; 0.05). The supplementation of VC (FM20C) increased the contents of total collagen, heat-insoluble collagen and free flavor amino acids, and decreased flesh lactic acid, malondialdehyde contents and steaming loss (<em>P</em> &lt; 0.05). The combined supplementation of Hyp and VC (FM20HC) showed higher total collagen and heat-insoluble collagen contents than the individual supplementation (FM20H and FM20C) (<em>P</em> &lt; 0.05), and the myofiber density, flesh hardness and shear force in the FM20HC group were also significantly higher than those in the control (<em>P</em> &lt; 0.05). In summary, the combined supplementation of hydroxyproline and VC in practical diet with fishmeal content of 200 g/kg significantly improved the growth performance and flesh quality of <em>L. vannamei</em> cultured in low salinity water.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 442-451"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different types of dietary carotenoids improve the color and odor quality of Eriocheir sinensis ovaries","authors":"Long Zhang ,&nbsp;Jingjing Wu ,&nbsp;Xiaodong Jiang ,&nbsp;Xugan Wu ,&nbsp;Xichang Wang","doi":"10.1016/j.aaf.2024.01.001","DOIUrl":"10.1016/j.aaf.2024.01.001","url":null,"abstract":"<div><div>Chinese mitten crab, <em>Eriocheir sinensis</em>, is a commercially ecological crab species in China. Ovaries are one of the major edible parts for female <em>E. sinensis</em>, and their color and flavor primarily affect consumer acceptance. This study investigated the effects of different dietary carotenoids on the color and odor quality of adult female <em>E. sinensis</em> ovaries. The crab was fed the experiment diets with supplementation of 100 ×10⁻⁶ of β-carotene, astaxanthin, lutein, canthaxanthin and zeaxanthin, respectively, for 70 days, while the control diet without the supplementation of carotenoids. The results showed that different carotenoids generated an orange-red coloration on crab ovaries, and astaxanthin and canthaxanthin significantly increased the redness (<em>P</em> &lt; 0.05). All carotenoid groups had higher total carotenoids and fatty acid contents than the control group. Moreover, β-carotene significantly promoted ovarian carotenoid deposition, and lutein predominantly affected fatty acid accumulation (<em>P</em> &lt; 0.05). β-carotene and lutein groups accumulated more aroma components (alcohols, ketones, and aldehydes) by regulating the metabolism of carotenoid and fatty acids in ovaries. These results show that canthaxanthin is a potential astaxanthin substitute for promoting coloration, while β-carotene and lutein can improve the odor quality of the ovaries of adult female <em>E. sinensis</em>.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 475-484"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139888465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity and therapeutical impacts of Bee venom (Apis mellifera L.) on Nile tilapia juvenile (Oreochromis niloticus)","authors":"Mohammed F. El Basuini ,&nbsp;Haitham M. Ramadan ,&nbsp;Abdelaziz M. El-Hais ,&nbsp;Mohamed A.A. Zaki ,&nbsp;Nourhan M. Kamel ,&nbsp;Islam I. Teiba ,&nbsp;Emad H. El-Bilawy ,&nbsp;Mohamed Reda Badr ,&nbsp;Mohamed Fathy Abdel-Aziz ,&nbsp;Akram Ismael Shehata","doi":"10.1016/j.aaf.2024.02.002","DOIUrl":"10.1016/j.aaf.2024.02.002","url":null,"abstract":"<div><div>Bee venom (BV) has been widely studied for its therapeutic properties in various medical applications. However, its effects on aquatic organisms, particularly Nile tilapia (<em>Oreochromis niloticus</em>), remain poorly understood, particularly concerning toxicity and therapeutic impacts. This study investigated the effects of bee venom (BV) injection on Nile tilapia (<em>O. niloticus</em>) by examining growth, feed efficiency, biometric parameters, whole-body chemical analysis, and the activity of antioxidant enzymes in the liver. Nile tilapia juveniles were injected with BV at doses of 0, 3, 6, 9, and 12 mg/kg of fish weight. Observations were made on the 14th day post-injection (DPI). No significant differences were found in growth, feed utilization, survival rate, biometric indices, and whole-body composition among the BV dosage groups. Similarly, the activity of intestinal enzymes (amylase, protease, and lipase) showed no significant changes. However, the study revealed a time-dependent response of antioxidant enzymes (SOD, CAT, and GPx) in the liver, with activity peaks on the 4th and 6th DPI. On the 14th DPI, fish injected with BV at doses of 3, 6, and 9 mg/kg exhibited higher antioxidant enzyme activities compared to the control and the highest dosage group (12 mg/kg). These results imply the presence of potential adaptive antioxidant activity and a favorable influence on the health and immune responses of Nile tilapia following BV injection. Notably, the effects appear to vary with the dose, influencing both antioxidant enzyme activities and immunity. Further research is warranted to explore the long-term effects of BV exposure and its potential therapeutic applications in aquaculture.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 429-441"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140090714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in rice blast resistance genes.","authors":"Zhan-Chun Wang, Gui-Tao Zhong, Bei-Bei Zhang, Yi-Lin Xie, Ding-Zhong Tang, Wei Wang","doi":"10.16288/j.yczz.24-358","DOIUrl":"https://doi.org/10.16288/j.yczz.24-358","url":null,"abstract":"<p><p>Rice (<i>Oryza sativa</i> L.) is an important food crop in China. Its growth and development are threatened by rice blast, which leads to huge grain losses in severe cases. Cultivating and deploying rice varieties that contain broad-spectrum resistance (<i>R</i>) genes is the most economical and effective strategy to prevent and control rice blast. In general, rice blast resistance genes code nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) that play a crucial role in the resistance to rice blast. Therefore, it is essential to clone <i>NLR</i> genes and elucidate the recognition and activation mechanisms between NLRs and effectors for rice disease resistance breeding. In this review, we summarize and provide perspectives on the research progress of <i>NLR</i> genes, the recognition between NLR proteins and effectors in rice, as well as the role and mechanism of paired NLR proteins in the resistance, hoping to provide reference for rice disease resistance breeding.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"533-545"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of regulatory mechanisms of enhancers in gastric cancer with double minute chromosomes based on bioinformatics.","authors":"Meng-Ting An, Guan-Lin Guo, Jie Wu, Wen-Jing Sun, Xue-Yuan Jia","doi":"10.16288/j.yczz.24-291","DOIUrl":"https://doi.org/10.16288/j.yczz.24-291","url":null,"abstract":"<p><p>Gastric cancer (GC) has a high incidence and mortality rate globally. Double minutes (DMs) are extrachromosomal circular chromosomes that carry amplified oncogenes or drug resistance genes, and they are closely associated with tumorigenesis and drug resistance. To investigate the role and regulatory mechanisms of double minutes in the malignant progression of gastric cancer, we utilize bioinformatics methods to analyze genomic copy number variation data from the CCLE cell line database and TCGA solid tumor database. We analyze and summarize the genomic features of tumor samples with double minutes. Based on these features, we classify gastric cancer samples. We use the FANTOM5 database, R package \"GenomicRanges\", Bedtools, and MEME SUITE to analyze the functions of the enhancers on double minutes and their regulated target genes in gastric cancer. Next, we apply the CIBERSORT package and the GDSC drug database to analyze immune cell infiltration and drug resistance in gastric cancer samples with and without double minutes from TCGA. The results show that the genome with a copy number greater than 10 and genomic fragments longer than 50 kb play a significant role. The regulatory role of double minutes enhancers affects drug resistance and tumor immunity, with disruptions in the enhancer-transcription factor-target gene regulatory loops also linked to tumor immunity. Furthermore, the target genes regulated by double minutes enhancers not only alter the expression of immune-related genes but also contribute to increased drug resistance to common chemotherapy agents in gastric cancer samples. High expression of drug resistance-related target genes in gastric cancer samples with double minutes is closely associated with poor prognosis. This study provides new insights for the treatment of gastric cancer patients with double minutes.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"558-572"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transposon insertion in pmel17 rewired skin and muscle transcriptomes in Mozambique tilapia","authors":"Fei Sun ,&nbsp;Le Wang ,&nbsp;Gen Hua Yue","doi":"10.1016/j.aaf.2023.12.002","DOIUrl":"10.1016/j.aaf.2023.12.002","url":null,"abstract":"<div><div>The <em>pmel17</em> gene plays a crucial role in melanin pigmentation. Our previous studies showed that in Mozambique tilapia, a transposon inserted into the 3′ untranslated region (3′ UTR) of <em>pmel17</em> resulted in the silencing of <em>pmel17</em> and led to the loss of melanin pigments (golden mutant phenotype). Additionally, the transposon insertion caused reduced growth performance and increased locomotion. In this study, to investigate the mechanisms underlying these phenotypic changes, we sequenced transcriptomes of the skin and muscle samples collected from wildtype and mutant tilapias. A total of 51 and 141 differentially expressed genes (DEGs) were identified in the skin and muscle transcriptomes, respectively. DEGs in the skin were primarily down-regulated in golden genotypes and associated with neural crest development and melanin pigmentation pathways. Besides these DEGs involved in the classic melanin pigmentation pathway of vertebrates, 14 DEGs were also observed to be related to melanogenesis. In muscle transcriptomes, there was an enrichment of GO terms associated with growth factors and cellular lipid catabolic processes. Specifically, DEGs related to growth factor binding exhibited a down-regulation, while those related to lipid metabolism showed an up-regulation in mutant genotypes. These findings agree with observed phenotypic changes. Furthermore, several DEGs associated with muscle function and mobility were up-regulated. Our study sheds light on how a single mutation in a gene can modulate multiple phenotypes by rewiring gene regulation networks. The research also provides valuable insights into the complex genetic mechanisms underlying the regulation of diverse phenotypic traits by a single gene.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 382-391"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Domestication of manggabai fish (Glossogobius giuris) through adaptation to different types of feed and aquaculture containers","authors":"Yuniarti Koniyo,&nbsp;Juliana","doi":"10.1016/j.aaf.2023.12.005","DOIUrl":"10.1016/j.aaf.2023.12.005","url":null,"abstract":"<div><div>This study aims to determine the growth and survival of Manggabai fish (Glossogobius giuris) cultivated in different types of feed and maintenance containers. The method used in the study was an experimental method using a 2 × 3 Factorial Complete Randomized Design (CRD), namely two levels of rearing container factors and three levels of feed type factors. The container consists of two types of containers, namely aquarium and concrete tub. The feed given consists of three types, namely <em>Daphnia</em> sp., <em>Tubifex</em> sp. And pellets. The research variables consisted of growth and survival of test animals. The test animals used were Manggabai fish with a total length of (3 ± 0.05) cm and body weight of 4 ± 0.05 g. Data analysis in this study used a two-factor Completely Randomized Design with a probability level of <em>P</em> &lt; 0.05 and used descriptive analysis of length, weight, and survival growth data during the maintenance of test animals. The results showed that the use of different feed and maintenance containers had a significant effect on the growth and survival of manggabai fish. Total absolute weight growth of manggabai fish reared in concrete containers and fed with <em>Daphnia</em> sp. 22.19 g, pellets 22.99 g, and those fed with <em>Tubifex</em> sp. Feed amounted to 39.63 g. The highest growth and survival were obtained in the treatment using a concrete tank and <em>Tubifex</em> sp. Feed. The highest growth and survival were obtained in the treatment using a concrete tank and <em>Tubifex</em> sp. Feed. The use of aquarium maintenance containers and the provision of pellets is not recommended for domestication of Manggabai fish, because it provides the lowest growth and survival.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 514-521"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139455791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding size selectivity of trawls using structural models: Methodology and a case study on fish sorting grids","authors":"Nadine Jacques ,&nbsp;Bent Herrmann ,&nbsp;Jesse Brinkhof ,&nbsp;Manu Sistiaga","doi":"10.1016/j.aaf.2024.03.003","DOIUrl":"10.1016/j.aaf.2024.03.003","url":null,"abstract":"<div><div>Fish behaviour affects the performance of selection devices in fishing gears. Traditionally, fish behaviour in relation to selection devices is assessed by direct observation. However, this approach has limitations, and the observations are not explicitly incorporated in the selectivity models. Further, underwater observations and quantification of fish behaviour can be challenging. In this study we outline and use an indirect method to explicitly incorporate and quantify fish behaviour in trawl selectivity analysis. We use a set of structural models, which are based on modelling the actual processes believed to determine the size selection of the device, to discern which behaviours are most likely to explain the selectivity process. By bootstrapping we assess how confident we can be in the choice of a specific structural model and on discerning the associated behavioural aspects. We collected size selectivity data in the Barents Sea demersal trawl fishery targeting gadoids, where the use of a sorting grid is compulsory. Using our modelling approach, we obtained deeper understanding of which behavioural processes most likely affect size selectivity in the sorting grids tested. Our approach can be applied to other fishing gears to understand and quantify fish behaviour in relation to size selectivity.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 499-513"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential contribution of Indonesian fishing vessels in reducing Green House gas emission","authors":"Suryanto Suryanto,&nbsp;Duto Nugroho,&nbsp;Umi Muawanah,&nbsp;Setiya Triharyuni,&nbsp;Dian Oktaviani,&nbsp;Sandi Wibowo,&nbsp;Novi Susetyo Adi,&nbsp;Puput Dani Prasetyo Adi","doi":"10.1016/j.aaf.2024.08.002","DOIUrl":"10.1016/j.aaf.2024.08.002","url":null,"abstract":"<div><div>In common practices, fishing vessels' Greenhouse Gas (GHG) emission is a part of the energy sector. Annual series data show Indonesian fishing vessels are growing by more than 18,000 units/year, dominated by fishing vessels with a tonnage of less than 30 GT. A ′bottom-up' approach was applied to develop low-carbon fishing activity practices to contribute to GHG emission reduction. This study focuses on a GHG inventory of fishing vessels managed by local and central Governments, using data from fishing permits and vessel monitoring systems from 2010 to 2018. The result is expected to act as the baseline for estimating emissions of fishing vessels up to 2030. The model predicted it could be reduced in 2030 by up to 6.43 E+07 tons CO2eq (68%) for fishing vessels with a size of less than 30 GT and up to 5.80E+06 tons CO2eq (84.5%) for a larger size of fishing vessels, respectively. To achieve further mitigation targets, mixing diesel oil using 30% biodiesel should be implemented progressively by 10% annually from 2021. Concerning fisher welfare after 2015, it shows a positive trend along with energy efficiency, and it can be concluded that strategies to improve the energy efficiency of active fishing vessels should be considered urgent.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"10 3","pages":"Pages 372-381"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and application of unstable genetically resistant strains in fission yeast.","authors":"Lu-Feng Dan, Yi-Wen Chu, Xin-Rong Wang, Xiang-Wei He","doi":"10.16288/j.yczz.24-266","DOIUrl":"https://doi.org/10.16288/j.yczz.24-266","url":null,"abstract":"<p><p>Reversible alterations at DNA sequence or epigenetic levels can result in phenotypes that are unstably inherited. The reversibility of these inheritable changes might be uniquely beneficial for adaption to possible fluctuations in environment. However, unstable changes are always ignored for the genetic instability in traditional studies, especially in the cause of drug resistance. In this study, we conduct a specific genetic screen in fission yeast using rapamycin (+caffeine) and obtain 173 resistant isolates. In contrast to the common strategy of isolating stable genetic mutants, we passage the cell culture with rapamycin resistance on drug free condition and test the resistance of offspring every five days, and obtain 14 strains that exhibit unstable resistance to rapamycin (the drug resistance is lost randomly among the cell progenies without drug selection pressure). Further studies show that the unstable genetic resistance of some strains is regulated by reversible DNA sequence alterationat the <i>ssp1</i> gene locus. This study provides new insights and relevant scientific basis for the regulatory mechanism of unstable drug resistance in the process of rapamycin as a clinical anti-tumor drug, and a new possible target for solving the problem of drug resistance.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 5","pages":"589-599"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}