农学最新文献

{"title":"Progress on the mining of functional genes of <i>Lonicera japonica</i>.","authors":"Nan Sun, Lu-Yao Huang, Sheng Yang, Jia Li, Cong-Zhe Hou, Zhen-Hua Liu","doi":"10.16288/j.yczz.24-190","DOIUrl":"10.16288/j.yczz.24-190","url":null,"abstract":"<p><p><i>Lonicera japonica</i> Thunb. is a semi-evergreen climbing shrub belonging to the Caprifoliaceae family, whose dried flower buds or flowers on the verge of blooming are known as Jin Yin Hua in traditional Chinese medicine. This plant is not only a high-value and widely used medicinal material but also possesses characteristics that make it suitable for both medicinal and culinary purposes. Currently, there is a robust market demand for Jin Yin Hua, yet the breeding technology for new varieties of <i>Lonicera japonica</i> lags behind, necessitating the integration of modern breeding techniques. With the advancement of genomics in <i>Lonicera japonica</i>, an increasing number of functional genes have been identified, amassing a rich reservoir of genetic resources for molecular breeding of this species. In this review, we summarize the progress in <i>Lonicera japonica</i> genomics, functional gene mining, and the establishment of genetic transformation systems. In light of the existing challenges and deficiencies in the research of functional genes and quality breeding of <i>Lonicera japonica</i>, it is imperative to establish a germplasm resource bank, a mutant library, and an efficient genetic transformation system for this plant. Intensive research into the mining and identification of functional genes should be conducted, and molecular markers closely linked to the functional genes of <i>Lonicera japonica</i> should be developed. This will lay a foundational basis for the identification and cultivation of breakthrough varieties with superior qualities in <i>Lonicera japonica</i>.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"920-936"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis and selection of high oleic peanut varieties in China: A study on agronomic, yield, and quality traits","authors":"Fangping Gong ,&nbsp;Zhuo Li ,&nbsp;Xiaojian Sun,&nbsp;Yi Fan,&nbsp;Yinghui Liu,&nbsp;Yurong Dang,&nbsp;Hao Li,&nbsp;Xingguo Zhang,&nbsp;Xingli Ma,&nbsp;Zhongfeng Li,&nbsp;Kai Zhao,&nbsp;Dongmei Yin","doi":"10.1016/j.ocsci.2024.09.001","DOIUrl":"10.1016/j.ocsci.2024.09.001","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) is an important oil crop. Oleic acid is a major factor that determines the quality of peanuts. Therefore, the high oleic and high oleic to linoleic acid ratio are the target traits in an advanced peanut breeding program. This study provided an extensive evaluation of the genetic and physical characteristics as well as disease resistance of 220 high oleic peanut varieties in China. Notably, these varieties clustered into five major categories based on their traits. A majority of these varieties have been bred using interspecific hybridization or selected from mutants of self-crossed parents, with the main parent varieties being Kaixuan 016 and CTWE. Analysis of disease resistance showed that most high oleic peanut varieties could resist two or three diseases. However, those varieties with resistance to multiple diseases were relatively scarce. Moreover, some high oleic peanut varieties showed no disease resistance or inadequate testing. The results further indicate that the genetic basis for high oleic peanut breeding is insufficient, highlighting the need for its further development. Importantly, our findings lay a critical foundation for future high oleic peanut breeding and promote better understanding of the genetic and trait diversity offered by these varieties.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 265-274"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Used vs. new: Does it have consequences for the performance of fishing gear?","authors":"","doi":"10.1016/j.aaf.2023.03.003","DOIUrl":"10.1016/j.aaf.2023.03.003","url":null,"abstract":"<div><div>Sea trials to test size selectivity equipment in trawls are often limited in time because they are costly, and the results can be influenced by multiple factors that are often overlooked. In the Barents Sea gadoid trawl fishery, the use of a size sorting grid in front of the codend is compulsory. The flexigrid, a netting section containing two flexible grids, is the most widely used grid section in this fishery. However, earlier selectivity studies with this device have shown inconclusive results. It has been speculated that the differences observed resulted from the difference in age and usage of the grid sections in the studies compared. To reveal whether potential changes in the device construction over time can lead to differences in size selection properties, we performed comparative fishing trials where we compared a brand new flexigrid section and a well-used flexigrid section used continuously by a commercial trawler for approximately four years. The results showed that the new flexigrid released significantly more cod below ∼60 cm than the used flexigrid. However, when the grids were fished with a subsequent diamond mesh codend, there was no difference in the overall selectivity of the two gears, meaning that the size selectivity in the codend compensates for the potential reduction in selectivity performance of the grids. This study shows the importance of considering the age and earlier use of size selection devices like sorting grids before they are compared with other devices, as their size selection properties can change significantly over time and with use.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 981-988"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74943537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal effort, fish farming, and marine reserve in fisheries management","authors":"","doi":"10.1016/j.aaf.2023.03.001","DOIUrl":"10.1016/j.aaf.2023.03.001","url":null,"abstract":"<div><div>In this paper, we develop a bioeconomic model to determine an optimal wild catch harvest and freshwater commercial aquaculture production in a lake that uses cages or floating rafts. Although off-bottom aquaculture activities have less impact on the environment compared to on-bottom aquaculture, it has negative externalities. It takes away the fishing area and hence creates pressure in the open-access fishing ground. It also changes the nutrient and ecological composition of the environment. We recommend the implementation of a reserved area to minimize the impact of fishing and aquaculture activities. The reserve also helps in improving the habitat and restocking the fish population which is declining rapidly. We determine the optimal sizes of the aquaculture and reserve and the optimal effort level that maximizes social welfare. We illustrate our findings using numerical simulations and perform an analysis of the optimal solutions with respect to biological and economic parameters involved in the model.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 975-980"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84875970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-localization signals enhance mitochondrial targeted presentation of engineered proteins.","authors":"Bing-Qian Zhou, Shang-Pu Li, Xu Wang, Xiang-Yu Meng, Jing-Rong Deng, Jin-Liang Xing, Jian-Gang Wang, Kun Xu","doi":"10.16288/j.yczz.24-171","DOIUrl":"10.16288/j.yczz.24-171","url":null,"abstract":"<p><p>Effective delivery of engineered proteins into mitochondria is of great significance for developing efficient mitochondrial DNA editing tools and realizing accurate treatment of mitochondrial diseases. Here, the candidate genes, <i>eGFP</i> and <i>Cas9</i>, were engineered with different mitochondrial localization signal (MLS) sequences introduced at their up- or/and down-streams. The corresponding expression vectors for the engineered proteins were constructed respectively, and HEK293T cells were transfected with these vectors. The fluorescence colocalization and Western blotting assays were used to analyze the mitochondrial targeting presentation effect of different engineered proteins. The results demonstrated that the daul-MLS modification of the eGFP and Cas9 proteins significantly improved the efficiency of mitochondrial targeted presentation, compared with the engineered proteins with single MLS added. Hence, it is speculated that dual MLS strategy can enhance the mitochondrial targeting of engineered proteins, which lays a theoretical foundation for the future development of efficient mitochondrial DNA editing tools.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"937-946"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of deacidification processes on the quality and oxidative stability of walnut oil","authors":"Xuan Ma ,&nbsp;Chongbo Huang ,&nbsp;Chang Zheng ,&nbsp;Fangyan Long ,&nbsp;Mandi Zhao ,&nbsp;Changsheng Liu","doi":"10.1016/j.ocsci.2024.10.001","DOIUrl":"10.1016/j.ocsci.2024.10.001","url":null,"abstract":"<div><div>In order to select an appropriate deacidification process and improve the quality of walnut oil, low-temperature cold-pressed crude walnut oil was used as raw material. Deacidified walnut oil was prepared using three deacidification processes: chemical deacidification (CD), adsorption deacidification (AD), and molecular distillation deacidification (MDD). The physicochemical properties, nutritional components, and <em>in vitro</em> antioxidant activities of the resulting deacidified walnut oils were comparatively analyzed. The results indicate that the fatty acid content in walnut oil exhibits fluctuating changes during the three different deacidification processes. The MDD shows a higher deacidification rate, reaching 94.06%, which is superior to the other two methods. Additionally, the AD retains more total phenols and tocopherols, with retention rates of 95.79% and 74.62%, respectively; whereas MDD is more effective at retaining phytosterols, achieving a retention rate of 98.09%. All these methods displayed positive impacts on the <em>in vitro</em> antioxidant capacity and oil stability of walnut oil, with ferric-reducing antioxidant power (FRAP) content and oxidative stability time were significantly reduced.whencompared to the untreated crude oil Among them, AD had the greatest impact on oxidative stability index (OSI), with its decreasing from 2.06 ​h to 0.82 ​h. Overall, compared to CD or MDD, the AD has best application prospects in preserving nutritional components.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 247-254"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of Recirculating Aquaculture System (RAS) with combination of clear water, probiotic and biofloc technology: A review","authors":"","doi":"10.1016/j.aaf.2023.07.006","DOIUrl":"10.1016/j.aaf.2023.07.006","url":null,"abstract":"<div><div>Recirculating Aquaculture System (RAS) is introduced in aquaculture farming industry to reduce water resource utilization, efficient the energy and land uses, and also help minimalize the water exchange. This system enables utilization of unsuitable land and promotes a sustainable environment in aquaculture industry. Furthermore, this technology has been established and proved efficient in monitoring the aquatic animal condition subsequently helps in maintaining the water quality and help remove solid particle wastes from the aquaculture treatment. As today, RAS has been developed with more effective technologies such as the use of UV irradiation, solid capture, protein skimmer and also provided with highly techno bio-filtration set. Basically, this system was applied for broodstock maturation, nursery phase, and grow-out production. In this review article, we provide an overview of RAS between the clear water, probiotic, and biofloc technology, and the advantages of its combination. Even though RAS and biofloc is two different parallel system, the application of the probiotic and biofloc in the semi-RAS application system is intense to be investigated. The synergistic effect of RAS using this combination towards high yield aquaculture production will be highlighted in this review paper. Expectantly this review paper will generate awareness and useful information on the RAS application in the aquaculture system operation with help in maximize the impact to the aquaculture yield production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 883-892"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73378125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofloc system supplemented by Pseudoalteromonas piscicida 1Ub protects the Pacific white shrimp Penaeus vannamei from Vibrio parahaemolyticus infection","authors":"","doi":"10.1016/j.aaf.2023.05.003","DOIUrl":"10.1016/j.aaf.2023.05.003","url":null,"abstract":"<div><div>This study aimed to evaluate the supplementation of the probiotic <em>Pseudoalteromonas piscicida</em> 1Ub to the biofloc system as an ecofriendly strategy for protecting white shrimp (<em>Penaeus vannamei</em>) from <em>Vibrio parahaemolyticus</em> infection. Shrimp with an average body weight of (0.50 ± 0.09) g were reared in 30 glass jars with a working volume of 2.5 L at a density of 20 ind/L. Shrimp were reared for 5 d for each treatment, which included the biofloc system without and with 10⁶ colony forming unit (CFU) per mL probiotic. The regular clear water system was used as control. All treatment groups were challenged with 10³, 10⁵, and 10⁷ CFU/mL <em>V. parahaemolyticus</em>. For the negative control, shrimp were reared without <em>V. parahaemolyticus</em>. The results showed that the density of <em>V. parahaemolyticus</em> cocultured with <em>P. piscicida</em> 1Ub decreased and the density of <em>V. parahaemolyticus</em> in rearing water and shrimp body in the probiotic-treated group was lower than that in the control group (<em>P</em> &lt; 0.05). The survival and immune response (total hemocyte count, phagocytic activity, respiratory burst, phenoloxidase, and superoxide dismutase) of shrimp in the probiotic group was higher than that in the positive control (<em>P</em> &lt; 0.05). Moreover, supplementing the biofloc system with the probiotic could protect shrimp hepatopancreas from damage caused by <em>V. parahaemolyticus</em>, regardless of bacterial density. Thus, the supplementation of the probiotic <em>P. piscicida</em> 1Ub in the biofloc system could significantly protect and increase the resistance of shrimp to <em>V. parahaemolyticus</em> infection.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 967-974"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74924612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug resistance mechanism of anti-angiogenesis therapy in tumor.","authors":"Xu Yan, Ying Guo, Dong-Lin Sun, Nan Wu, Yan Jin","doi":"10.16288/j.yczz.24-110","DOIUrl":"10.16288/j.yczz.24-110","url":null,"abstract":"<p><p>Angiogenesis refers to the process of forming a new network of blood vessels from existing ones through the migration, proliferation, and differentiation of endothelial cells. This process is crucial for the growth and spread of solid tumors, particularly once the tumor volume exceeds 2 mm³, as the newly formed vascular network provides essential oxygen, nutrients, and growth factors to the tumor. Anti-angiogenesis therapy has become one of the commonly used targeted treatments for cancer in clinical practice. Bevacizumab, the first anti-angiogenesis drug, has been widely applied in the treatment of various solid tumors. However, due to acquired resistance, its efficacy is typically sustained for only 1 to 2 years. Despite the relative genomic stability of endothelial cells, which makes resistance less likely, various types of resistance phenomena have been observed in clinical practice, indicating that resistance to anti-angiogenic therapy remains a challenging research area. This review focuses on the latest advances in the mechanisms of resistance to anti-angiogenic therapy in tumors and explores new prospects for anti-tumor angiogenesis treatment, in order to provide strong theoretical support and guidance for clinical practice.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"911-919"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-myc modulates the replication of RGNNV via glutamine-mediated ATP production in grouper fin cells","authors":"","doi":"10.1016/j.aaf.2023.05.005","DOIUrl":"10.1016/j.aaf.2023.05.005","url":null,"abstract":"<div><div><em>C-myc</em> is a proto-oncogene that plays an important role in a variety of diseases. There were a lot of research on the correlation between C-myc and human viruses. However, the study about <em>C-myc</em> related to aquatic species virus is very limited. In the present study, the qRT-PCR, cellular immunofluorescence and western blotting determination data reported that <em>C-myc</em> and glutaminase (<em>GLS</em>) genes were significantly upregulated when grouper fin cells (GF-1) were infected with red grouper nervous necrosis virus (RGNNV). After knocking down the <em>C-myc</em> gene, the mRNA and protein levels of <em>GLS</em>, capsid protein (<em>CP</em>) and RNA polymerase (<em>RdRp</em>) of RGNNV were significantly reduced in RGNNV-infected GF-1 cells and the overexpression of the <em>C-myc</em> gene remarkably promoted these genes, which indicated that the replication of the virus and <em>GLS</em> gene were positively regulated by <em>C-myc</em> in RGNNV-infected GF-1 cells. In addition, supplementation of exogenous ATP can partially restore viral replication when RGNNV-infected GF-1 cells were cultured in glutamine-free medium, which confirmed that the glutamine was decomposed into ATP to provide energy for viral replication. Further studies confirmed that overexpression of <em>C-myc</em> can increase the content of ATP in normal cells. To sum up, these data suggested that activation of <em>C-myc</em> gene affected viral replication by regulating <em>GLS</em> expression to drive glutamine dissolution.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 929-936"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82520737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}