农学最新文献

{"title":"Study on population genomics of <i>Bacillus anthracis</i> based on multiple types of genetic variations.","authors":"Zu-Ming Zhang, Hao Zhou, Xue-Zhi Huang, Duo-Yue Zhang, Jia-Yi Zhang, Yu Lin, Li-Wei Fang, Xiu-Chang Zhang, Yu-Jun Cui, Ya-Rong Wu, Yan-Jun Li","doi":"10.16288/j.yczz.24-295","DOIUrl":"https://doi.org/10.16288/j.yczz.24-295","url":null,"abstract":"<p><p><i>Bacillus anthracis</i>, the causative agent of the deadly infectious disease anthrax, is also a typical biological warfare agent. It primarily infects livestock such as cattle and sheep, as well as humans, causing significant economic losses to the livestock industry and posing a threat to human society. A more profound insight into the genetic diversity and evolutionary drivers of this species is essential for studying its virulence mechanisms and conducting anthrax surveillance and control. However, current research in this area is insufficient, particularly lacking population genomic studies based on multiple types of genetic variation. In this study, we collect and filter the genome sequences of 1,628 publicly available <i>B. anthracis</i> strains, and identify various types of variation in 1,347 high-quality sequences, including SNPs, indels, large fragment gains and losses, copy number variations (CNVs), and genome rearrangements. In total, we identify 26,635 SNPs, 9,997 indels, 21 large fragment gains and losses, 25 CNVs, and 5 inversions. Phylogenetic reconstruction reveals that this species can be divided into six major populations and 17 subgroups. By integrating population diversity and geographic distribution characteristics, we find that U.S. strains exhibit the highest genetic diversity, while African strains show significant geographic clustering. Additionally, through selection pressure analysis, we identify strong selection signals in four genes (<i>rpoB, fusA, spo0F</i>, <i>GBAA</i>_<i>RS11385</i>) related to drug resistance and sporulation. This study reconstructs the global population structure of <i>B. anthracis</i> and reveals key variations during the species' evolutionary process, providing targets for anthrax strain identification, tracing, and virulence mechanism research, as well as scientific support for the prevention and control of anthrax outbreaks.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"681-693"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onsite rapid detection method for genetically modified maize and soybean based on recombinase polymerase amplification.","authors":"Jia-Tong Yan, Guan-Wei Chen, Qing-Mei Miao, Cheng Peng, Lei Yang, Xiao-Yun Chen, Xiao-Li Xu, Wei Wei, Jun-Feng Xu, Xiao-Fu Wang","doi":"10.16288/j.yczz.24-322","DOIUrl":"https://doi.org/10.16288/j.yczz.24-322","url":null,"abstract":"<p><p>With the commercial planting of transgenic maize and transgenic soybeans in China, in order to ensure the healthy development of China's biological breeding industry, it is urgent to develop on-site rapid detection techniques for transgenic maize and soybeans. In this study, a rapid detection method based on recombinase polymerase amplification (RPA) was proposed to overcome the shortcomings of on-site rapid detection of transgenic maize and soybean. Representative transgenic maize (Shuang Kang 12-5, DBN9936, MON810) and soybean (DBN9004, GTS40-3-2, SHZD32-1) were selected as test objects. According to their flanking sequence, specific primers and probes were designed and screened, and a RPA amplification system with high specificity and sensitivity of 20 copies for each transgenic event was established. In order to quickly obtain DNA, seed samples use nucleic acid release agents to obtain templates that can be used for RPA amplification. In order to eliminate the interference of chlorophyll in leaves on RPA fluorescence signal and quickly obtain DNA, leaf samples use the nucleic acid rapid integrated extraction device developed by our laboratory to quickly obtain DNA and eliminate the chlorophyll interference. Combined with a portable metal bath for initiating RPA amplification, a blue light gel cutter for visual detection, and a rapid on-site detection method for transgenic maize and soybean was constructed. The entire detection process required approximately 20 minutes, and the detection result was consistent with the result of conventional PCR. It is suitable for on-site rapid detection without large equipment, and has strong practicability and adaptability. The proposed approach provides technical support for on-site detection of transgenic crops and offers a reference for the application of other rapid nucleic acid detection technologies.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"47 6","pages":"694-707"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: <i>PpTCP18</i> is upregulated by lncRNA5 and controls branch number in peach (<i>Prunus persica</i>) through positive feedback regulation of strigolactone biosynthesis.","authors":"","doi":"10.1093/hr/uhaf139","DOIUrl":"https://doi.org/10.1093/hr/uhaf139","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1093/hr/uhac224.].</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"12 7","pages":"uhaf139"},"PeriodicalIF":7.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12235252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation of <i>ERECTA</i> homologous genes confers ideal plant architecture in <i>Brassica napus</i>.","authors":"Hui Zhang, Tao Wang, Jianwei Gu, Dengfeng Hong","doi":"10.1007/s42994-025-00217-4","DOIUrl":"https://doi.org/10.1007/s42994-025-00217-4","url":null,"abstract":"<p><p>Varieties with a semi-dwarf compact plant architecture may increase yield per unit area in rapeseed (<i>Brassica napus</i>) by allowing high-density cultivation and mechanical harvesting while conferring lodging resistance. Mutation of <i>ERECTA</i> (<i>ER</i>), which encodes a receptor-like protein kinase, generates a compact and upright plant architecture in <i>Arabidopsis thaliana</i>; however, there have been no reports on the roles of the ER family (ERf) in <i>B. napus</i>. In this study, we used the CRISPR/Cas9 system to generate mutants in each of the two homoeologs of <i>B. napus ERf</i> members <i>BnaER</i> and <i>ER-Like 1</i> (<i>BnaERL1</i>), and in the single <i>BnaERL2</i> gene, resulting in the homozygous mutants <i>BnaA09.er/BnaC08.er</i>, <i>BnaA06.erl1</i>/<i>BnaC03.erl1</i>, and <i>BnaA10.erl2</i>. Under greenhouse conditions, <i>BnaA09.er</i>/<i>BnaC08.er</i> plants were shorter than the wild type, with a compact inflorescence and shorter siliques. In addition, <i>BnaA09.er</i>/<i>BnaC08.er</i> plants produced significantly more branches and total siliques than the wild type, with no significant changes in the number of ovules per silique or thousand-seed weight. Under field conditions, the <i>BnaA09.er/BnaC08.er</i> mutant plant showed a phenotype comparable to that under greenhouse conditions, but with a notable drop in thousand-seed weight. These results indicate that the <i>BnaA09.er/BnaC08.er</i> mutant offers a valuable germplasm resource for breeding rapeseed with ideal plant architecture.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00217-4.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"249-262"},"PeriodicalIF":4.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observe natural selection by evolutionary experiments in crops.","authors":"Tian Wu, Shifeng Cheng","doi":"10.1007/s42994-025-00215-6","DOIUrl":"10.1007/s42994-025-00215-6","url":null,"abstract":"<p><p>Evolutionary experiments provide a unique lens through which to observe the impacts of natural selection on crop evolution, domestication, and adaptation through empirical evidence. Enabled by modern technologies-such as the development of large-scale, structured evolving populations, high-throughput phenotyping, and genomics-driven genetics studies-the transition from theoretical evolutionary biology to practical application is now possible for staple crops. The century-long Barley Composite Cross II (CCII) competition experiment has offered invaluable insights into understanding the genomic and phenotypic basis of natural and artificial selection driven by environmental adaptation during crop evolution and domestication. These experiments enable scientists to measure evolutionary dynamics, in real time, of genetic diversity, adaptation of fitness-associated traits, and the trade-offs inherent in selective processes. Beyond advancing our understanding of evolutionary biology and agricultural practices, these studies provide critical insights into addressing global challenges, from ensuring food security to fostering resilience in human societies.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"381-387"},"PeriodicalIF":4.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agricultural biotechnology in China: product development, commercialization, and perspectives.","authors":"Jingang Liang, Yu Sun, Yanchao Yang, Zeyu Wang, Han Wu, Taotao Gu, Ruifu Zhang, Xinli Sun, Bin Yao, Tao Tu, Xiaoqing Liu, Huiying Luo, Guangzhi Tong, Yue Jiao, Kui Li, Jie Zhang, Kongming Wu","doi":"10.1007/s42994-025-00209-4","DOIUrl":"10.1007/s42994-025-00209-4","url":null,"abstract":"<p><p>Meeting the increasing demand for food and industrial products by the growing global population requires targeted efforts to improve crops, livestock, and microorganisms. Modern biotechnology, particularly genetic modification (GM) and genome-editing (GE) technologies, is crucial for food security and environmental sustainability. China, which is at the forefront of global biotechnological innovation and the rapid advancements in GM and GE technologies, has prioritized this field by implementing strategic programs such as the National High-tech Research & Development Program in 1986, the National Genetically Modified Organism New Variety Breeding Program in 2008, and the Biological Breeding-National Science and Technology Major Project in 2022. Many biotechnological products have been widely commercialized in China, including biofertilizers, animal feed, animal vaccines, pesticides, and GM crops such as cotton (<i>Gossypium hirsutum</i>), maize (<i>Zea mays</i>), and soybean (<i>Glycine max</i>). In this review, we summarize progress on the research and utilization of GM and GE organisms in China over the past 3 decades and provide perspectives on their further development. This review thus aims to promote worldwide academic exchange and contribute to the further development and commercial success of agricultural biotechnology.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"284-310"},"PeriodicalIF":4.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth-promoting rhizobacteria on peanut seedlings (Arachis hypogaea L.): Isolation, taxonomical, and functional characterization","authors":"Ezequiel Darío Bigatton ,&nbsp;Ibrahim Ayoub ,&nbsp;María Ángeles Castillejo ,&nbsp;Carolina Merlo ,&nbsp;Carolina Vázquez ,&nbsp;Mariela Valeria Archilla ,&nbsp;Marina Bruno ,&nbsp;María Paula Martín ,&nbsp;Romina Paola Pizzolitto ,&nbsp;Lucas Esteban Dubini ,&nbsp;Enrique Iván Lucini ,&nbsp;Ricardo Javier Haro","doi":"10.1016/j.ocsci.2025.04.003","DOIUrl":"10.1016/j.ocsci.2025.04.003","url":null,"abstract":"<div><div>To improve crop yields, global food production needs sustainable agronomic tools like Plant Growth-Promoting Rhizobacteria (PGPR). Region-adapted PGPR strains are crucial to increasing peanut production. Argentina is the seventh-largest peanut producer, and Córdoba is the main region with 250,000 ​ha (75% of the total sowing area). This study aimed to isolate, identify, and characterize the biocontrol and growth promotion capacity of PGPR strains belonging to the <em>Bacillus</em> and <em>Pseudomonas</em> genera. The strains were tested against <em>Sclerotinia minor</em>, <em>Sclerotium rolfsii</em>, <em>Fusarium verticillioides</em>, and <em>Aspergillus flavus</em> for biocontrol assays. For growth promotion, pot trials used two peanut cultivars, ASEM 400 INTA and Granoleico, under 40% and 60% field capacity under two water regimes. The isolated strains were <em>Bacillus velezensis</em>, <em>B. subtilis</em>, <em>B. tequilensis</em>, <em>B</em>. <em>safensis, B. altitudinis</em>, and <em>Pseudomonas psychrophila</em>. These strains demonstrated <em>in-vitro</em> phosphorus solubilization, nitrogen fixation, ammonification, nitrification, enzyme releasing, phytohormones production, and high biocontrol capacity of over 75%. SC6 and RI3 (both <em>B. velezensis</em>) and P10 (<em>P. psychrophila</em>) exhibited outstanding performance. They significantly promoted peanut root biomass by more than 50% and leaf area by 30%, with increased chlorophyll content index and leaf relative water content, particularly under water stress conditions (40% field capacity). According to the results, RI3, SC6, and P10 could be classified as PGPR, which supports the results obtained in other field studies with these same microorganisms. Future investigations should prioritize the development of industrial formulations to assess their effectiveness in alternative crops and to incorporate them into other agricultural practices.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 2","pages":"Pages 87-99"},"PeriodicalIF":0.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soyasaponin β-glucosidase confers soybean resistance to pod borer (<i>Leguminivora glycinivorella</i>).","authors":"Chengyong Feng, Xindan Xu, Jia Yuan, Mingyu Yang, Fanli Meng, Guodong Wang","doi":"10.1007/s42994-025-00214-7","DOIUrl":"10.1007/s42994-025-00214-7","url":null,"abstract":"<p><p>Plant specialized metabolites are commonly stored in glycosylated forms within plant cells, with their homeostasis regulated by glycosyltransferases and β-glucosidases (BGLUs, also known as β-glucoside hydrolases (E.C.3.2.1.21)). Soyasaponins, the predominant triterpenoid compounds (C30) in soybean seeds, contain two sugar moieties attached at the C3 and C22 positions. While glycosyltransferases involved in soyasaponin biosynthesis have been well characterized, the role of BGLUs in soyasaponin homeostasis remains unclear. In this study, we identified <i>GmSSBG1</i> (<i>Soyasaponin β-glucosidase1</i>; <i>Glyma.07G258700</i>) as a candidate gene potentially involved in soyasaponin homeostasis through gene to gene co-expression analysis. Biochemical assays demonstrated that GmSSBG1 specifically hydrolyzes arabinose residues at the C22 position of A0- and B0-series soyasaponins. Loss-of-function mutations in <i>GmSSBG1</i> led to a significant accumulation of A0- and B0-series soyasaponins in mutant seeds, which correlated with a pronounced decrease in resistance to the soybean pod borer (<i>Leguminivora glycinivorella</i>). Our findings provide critical insights into the regulatory mechanisms underlying soyasaponin homeostasis and lay a theoretical foundation for molecular breeding strategies aimed at developing soybean lines with enhanced resistance to soybean pod borer, even to other insect pests.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00214-7.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"160-173"},"PeriodicalIF":4.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterotrimeric G-protein subunits regulate plant architecture, pod development, seed size, and symbiotic nodulation in <i>Medicago truncatula</i>.","authors":"Fanghao Sun, Fugui Zhu, Shasha Ran, Qinyi Ye, Tao Wang, Jiangli Dong","doi":"10.1007/s42994-025-00210-x","DOIUrl":"10.1007/s42994-025-00210-x","url":null,"abstract":"<p><p>Heterotrimeric G proteins are crucial transducers of signaling from receptors, participating in growth and development, as well as in responses to biotic and abiotic stimuli. However, little is known about their roles in regulating various yield-related traits in legumes. In this study, we systematically analyzed the functions of two G-protein-encoding genes, <i>MtGα1</i> and <i>MtGβ1</i>, along with <i>Regulator of G-protein Signaling1</i> (<i>MtRGS1</i>), in <i>Medicago truncatula</i>. All three genes were ubiquitously expressed in roots, stems, leaves, nodules, flowers, and pods. We generated the knockout mutants <i>Mtgα1</i>, <i>Mtgβ1</i>, and <i>Mtrgs1</i> using CRISPR/Cas9 and assessed their growth and development. <i>MtGα1</i> knockout resulted in slightly shorter plants with smaller pods and shorter spines, but larger seeds, without affecting overall biomass or other traits. <i>MtGβ1</i> knockout led to dwarfism, weak root development, a severe drop in biomass production, smaller legume pods with shorter spines, and smaller seeds. However, the <i>Mtrgs1</i> mutants were largely similar to wild-type plants, with few significant defects in growth and development. We also investigated the symbiotic nodulation-related phenotypes of these mutants, discovering that <i>Mtgβ1</i> mutants produce lighter nodules, whereas <i>Mtgα1</i> and <i>Mtrgs1</i> mutants have normal nodulation phenotypes similar to those of wild-type plants. These observations suggest that MtGβ1 positively regulates nodulation, although the detailed mechanisms by which G proteins regulate symbiotic nitrogen fixation in <i>M. truncatula</i> remain to be explored. This work provides potentially valuable genetic resources for further functional analysis and elucidation of the molecular mechanisms of G proteins in this model legume.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00210-x.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"141-159"},"PeriodicalIF":4.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous knockout of multiple <i>eukaryotic translation initiation factor 4E</i> genes confers durable and broad-spectrum resistance to potyviruses in tobacco.","authors":"Yong Liu, Shuo Wang, Danyang Zhao, Chenglu Zhao, Haiqin Yu, Jianmin Zeng, Zhijun Tong, Cheng Yuan, Zhenghe Li, Changjun Huang","doi":"10.1007/s42994-025-00216-5","DOIUrl":"10.1007/s42994-025-00216-5","url":null,"abstract":"<p><p>Recessive resistance mediated by mutations in the eukaryotic translation initiation factor 4E (eIF4E), has proven effective against diverse potyviruses and is extensively utilized in breeding programs. However, the rise of resistance-breaking (RB) strains and emerging potyviral species necessitates the development of more durable and broad-spectrum resistance strategies. In this study, our field survey in Yunnan, China, identified potato virus Y (PVY) RB isolates, as well as the prevalence of tobacco vein banding mosaic virus (TVBMV) and chilli veinal mottle virus (ChiVMV), in tobacco carrying the recessive <i>va</i> locus, which lacks the <i>eIF4E1-S</i> susceptibility gene, due to a chromosomal deletion. Protein interaction and viral infection assays demonstrated that both eIF4E1-S and eIFiso4E-T are used by PVY RB as susceptibility factors for infection, with the combined inactivation of these genes confering durable resistance. Similarly, the knockout of <i>eIFiso4E-S,</i> in the <i>va</i> genetic background, provided effective resistance to TVBMV and reduced susceptibility to ChiVMV. Notably, pyramiding mutations in <i>eIFiso4E-S</i> and <i>eIFiso4E-T</i>, in <i>va</i> tobacco, generated plants exhibiting robust, broad-spectrum resistance, to all three viruses, without compromising plant development. These findings underscore the potential of stacking eIF4E mutations to engineer durable, broad-spectrum resistance to potyviruses in tobacco, offering a promising strategy for crop improvement.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00216-5.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"232-248"},"PeriodicalIF":4.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}