农学最新文献

{"title":"Stability analysis of reef fish communities based on symbiotic graph model","authors":"","doi":"10.1016/j.aaf.2023.03.005","DOIUrl":"10.1016/j.aaf.2023.03.005","url":null,"abstract":"<div><div>The community stability of coral reefs and fish is the focus of ecological monitoring of coral reefs. Among them, the realization of effective metrics of variations in reef fish communities (i.e., the combined communities of coral reefs and fish) is important for analyzing the stability of communities as well as maintaining the ecological balance of coral reefs. Based on coral reef and fish data collected at St. John's Island from 2004 to 2010, this study proposes a symbiotic graph modeling method to express the biological relationships of reef fish communities, and a Pyramid Match graph kernel method for fusing Attributes (PMA) to quantify community fluctuations to measure interannual variability of communities. The results showed that the community similarity was low in 2006, 2007, and 2008. The total coral cover rate in the study area decreased by 32.04% from 2006 to 2007 and increased by 24% in 2008. The total number of fish fell from 3780 in 2006 to 2596 in 2007 and rose to 6249 in 2008. Among them, the proportion of herbivorous fish decreased to 30.84% in 2007. Furthermore, we have combined the Louvain algorithm with the proposed PMA method to effectively identify the regions that should be prioritized for protection. Experiments were conducted on real datasets with good results, demonstrating the potential of the proposed method to assist in the analysis of community stability and identification of priority conservation areas.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 1007-1017"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72851961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of biological flocculation technology (BFT) on water quality dynamics and immune response of grass carp (Ctenopharyngodon idella)","authors":"","doi":"10.1016/j.aaf.2023.04.005","DOIUrl":"10.1016/j.aaf.2023.04.005","url":null,"abstract":"<div><div>The biofloc technology (BFT) offers benefits in improving feed utilization and reducing the feed conversion ratio compared to the recirculating aquaculture system (RAS). In this study, high-throughput 16S rRNA gene sequencing was employed to investigate and compare the bacterial communities in these aquaculture systems and the gut microbiota of grass carp reared in them. We observed a significant distinction between the microbial communities of the aquaculture systems and the intestinal microbiota of grass carp. The genera <em>cetobacterium</em> and <em>bacillus</em> were more abundant in the BFT system, accounting for only 0.05% and 0.25% in the RAS. Our study also demonstrated that BFT could influence the intestinal microorganisms of grass carp (<em>Ctenopharyngodon Idella</em>) by reducing the relative abundance of <em>burkholderia</em>-<em>caballeronia</em>-<em>paraburkholderia</em> and increasing that of <em>cetobacterium</em>. Based on bugBase community predictions, the potential pathogenicity in BFT and grass carp intestine was lower than in RAS. Regarding gene expression in the grass carp intestine, immunosuppressive genes showed higher expression, while pro-inflammatory genes exhibited lower expression in grass carp reared in BFT. Furthermore, the final average length and weight of grass carp in the BFT system were significantly higher than those in the RAS. This study provides valuable insights into gut microbiome ecology in relation to two grass carp aquaculture systems, which could be effectively applied in grass carp management to promote health and improve water quality.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 921-928"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90102894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of microbe-derived antioxidants on growth, digestive and aminotransferase activities, and antioxidant capacities in the hepatopancreas of Eriocheir sinensis under ammonia nitrogen stress","authors":"","doi":"10.1016/j.aaf.2022.12.002","DOIUrl":"10.1016/j.aaf.2022.12.002","url":null,"abstract":"<div><div>This study evaluated the effects of feeding a microbial-derived antioxidant diet (MA) for 56 days on the growth performance of Eriocheir sinensis, as well as on digestive and metabolic enzyme activity and antioxidant capacity under ammonia stress (400 mg/L ammonia nitrogen for 48 h). The weight gain at 28d and the specific growth rate at 56ds for crabs in the MA group were significantly higher than those in the control diet (C) group (<em>p</em> &lt; 0.05). Compared with the C group, the color of the hepatopancreas in the MA group was significantly improved (<em>p</em> &lt; 0.05). A significant decrease in the level of Hemolymph ammonia was found in the microbial antioxidant ammonia-N (MA_A) group (<em>p</em> &lt; 0.05), which may be related to the decrease of transaminase (AST, ALT, GDH) activities caused by MA diet (<em>p</em> &lt; 0.05). There is a significant increase in the digestive enzyme activities (Trypsin and Lipase), which reduced the accumulation of triglycerides in the hepatopancreas in the (MA_A) group (<em>p</em> &lt; 0.05). In addition, the antioxidant damage of crabs in hepatopancreas caused by ammonia nitrogen toxicity could also significantly be improved by MA fed in the (MA_A) group (<em>p</em> &lt; 0.05). microbe-derived antioxidants (supplemented with 0.2% in feeds) can protect the health of hepatopancreas, promote growth, and improve the ability to resist ammonia nitrogen stress for E.sinensis.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 957-966"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73160500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IKKε positively regulates NF-κB, MAPK, and IRF3-mediated type I IFN signaling pathways in Japanese eel (Anguilla japonica)","authors":"","doi":"10.1016/j.aaf.2023.01.001","DOIUrl":"10.1016/j.aaf.2023.01.001","url":null,"abstract":"<div><div>IKKε is an IκB kinase participating in the control of NF-κB and type I IFN signal pathways in mammals. However, the function of IKKε in regulating immune response is largely unknown in teleost. Herein, an IKKε homologue named <em>Aj</em>IKKε was characterized in Japanese eel (<em>Anguilla japonica</em>). <em>Aj</em>IKKε has an N-terminal kinase domain, a ubiquitin-like domain, and a coiled coil-containing domain (CC), which is conserved and similar to its counterpart in mammals. Expression analysis showed that <em>Aj</em>IKKε could be up-regulated in kidney, spleen, and particularly in liver under the stimulation of poly I:C, LPS, and <em>Aeromonas hydrophila</em> infection. <em>In vitro</em>, the mRNA levels of <em>Aj</em>IKKε were significantly provoked in eel liver cells stimulated by LPS and poly I:C, or the different concentrations of <em>A. hydrophila</em>. The overexpression of <em>Aj</em>IKKε could not only induce a significantly higher level of promoter activity of human NF-κB, AP-1, and IFN-β in a dose-dependent manner but also up-regulate the activation of promoters of Japanese eel cRel, AP1, IL6, IFN4, IRF3, and IRF7 in HEK293 cells. RNAi studies showed that after <em>Aj</em>IKKε was knocked down, the expression levels of IL1, IL6, TNFα, c-Jun, IFN2, IFN3, MX1, MX2, and IRF3 genes were significantly down-regulated in liver, spleen, and kidney of Japanese eels. In addition, the mutants of <em>Aj</em>IKKε-K39A, <em>Aj</em>IKKε-S174A, and <em>Aj</em>IKKε-ΔCC failed to activate Japanese eel IFN4, IRF3 and human IFN-β promoters in HEK293 cells. Collectively, these results suggest that <em>Aj</em>IKKε may function as a positive regulator of NF-κB, MAPK, and IRF3-mediated type I IFN signaling pathways related to immune response evoked by bacterial and viral infection.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 908-920"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78132520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A compact underwater stereo vision system for measuring fish","authors":"","doi":"10.1016/j.aaf.2023.03.006","DOIUrl":"10.1016/j.aaf.2023.03.006","url":null,"abstract":"<div><div>In the context of aquaculture, fish length is a key parameter to assess fish stocks, essential for feeding regime and contributes for decisions at several production levels. However, taking length measurements is a cumbersome task that, when applied to living individuals, can induce considerable stress, increasing the risk of damage or hindering their growth. Computer vision is one of the most used non-contact tools to overcome this issue, being fast, consistent and repeatable. However, its use in aquatic environments is limited by the high cost, the difficulty of calibrating the system in underwater conditions and the complexity of implementation. This paper proposes a low-cost easy-to-use vision system that can take measurements on live fish in aquatic conditions, without the need for a special calibration or a demanding in-water calibration service. The present work implemented a compact stereo vision system and developed a method that estimates the correct length of fish, based on the variation of the angle of incidence of the light rays in the water. Given some structural conditions such as a short baseline, the system is able to measure fish with an error of less than 1%. The short baseline allows to have a compact system and reduces the effect of water refraction on the 3D reconstruction. A set of experiments were performed with real fish, working robustly for a set of orientations of the fish (even when the caudal fin and snout are on different distances to the cameras).</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 1000-1006"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75300570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sturgeon and paddlefish: Review of research on broodstock and early life stage management","authors":"","doi":"10.1016/j.aaf.2023.04.001","DOIUrl":"10.1016/j.aaf.2023.04.001","url":null,"abstract":"<div><div>Sturgeon aquaculture has taken a parallel yet unique path relative to other teleost rearing. One of the driving factors has been the fact that virtually all extant species are facing extinction; thus, laws concerning the transport and processing of sturgeon are stringent. As a result, most sturgeon companies vertically integrate the farming process from hatchery to harvest, in contrast to the production of other commercial fish species (e.g., trout, sea bass, salmon), where farmers tend to purchase fry from producers specialized only in the production of that life stage and sell product to unaffiliated fish processors. Sturgeon aquaculture is furthermore unique in the extremely high value of the main product (caviar). The secondary product (meat) also surpasses other finfish aquaculture products' average price per weight. These expensive outputs reveal the greatest challenge in production — an exceptionally long time to market from larvae to harvestable adults. For all their rigor and robustness as juveniles and adults, sturgeon species are highly fragile during larval stages. Due to the unique legal framework around sturgeon cultivation, aquaculturists often specialize in regional species, although some hybrids have achieved global popularity. This lends to a heterogenous swath of literature on best cultivation and management practices, especially at early growth stages. Nonetheless, the role of aquaculture as both a food source and restocking program to thwart further extinction of sturgeon species has hardly been fully tapped. In light of the many challenges, this review examines recent advances in sturgeon farming with an emphasis on broodstock and early life-stage management to summarize studies that can help researchers and farmers assess the industry's current state.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 871-882"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82357053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of structural parameters on the hydrodynamic performance of vertical curved V-Type otter board","authors":"","doi":"10.1016/j.aaf.2023.02.004","DOIUrl":"10.1016/j.aaf.2023.02.004","url":null,"abstract":"<div><div>In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board, this paper based on the computational fluid dynamics method, a three-dimensional numerical model of vertical curved V-type otter board is established to calculate and analyze its hydrodynamic performance, and the validity of the numerical simulation results is verified by designing and constructing a scaled model for dynamic flume experiments. On this basis, the effects of different structural parameters, such as the deflector angle, camber, and spacing, on the hydrodynamic performance of the vertical curved V-Type otter board are investigated and the optimal structural design scheme is determined. The results demonstrate that single or synchronous changes of the angle, camber, and spacing of the two deflectors have varying degrees of impact on the hydrodynamic performance of the vertical curved V-Type otter board. To improve the expansion effect of the otter board, setting the angle of the deflector A to 40° and that of the deflector B to 30°, or setting the camber of deflectors A and B to 18%, or setting the spacing between deflectors A and B to 380 mm and that between the deflector and the main panel to 670 mm can increase the lift coefficient of the vertical curved V-Type otter board to its maximum value. Considering the overall performance of the otter board, when the angle of the deflector A is set to 25° and that of the deflector B is set to 30°, or the camber of the deflector A is set to 6% and that of the deflector B is set to 9%, or the spacing between deflectors A and B is set to 340 mm and that between the deflector and the main panel is set to 610 mm, the lift-to-drag ratio of the otter board can be increased to its maximum value.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 989-999"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90304361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural variations in oil crops: Types, and roles on domestication and breeding","authors":"Xiaobo Cui,&nbsp;Miao Yao,&nbsp;Meili Xie,&nbsp;Ming Hu,&nbsp;Shengyi Liu,&nbsp;Lijiang Liu,&nbsp;Chaobo Tong","doi":"10.1016/j.ocsci.2024.09.002","DOIUrl":"10.1016/j.ocsci.2024.09.002","url":null,"abstract":"<div><div>Structural variations (SVs), a newly discovered genetic variation, have gained increasing recognition for their importance, yet much about them remains unknown. With the completion of whole-genome sequencing projects in oil crops, more SVs have been identified, revealing their types, genomic distribution, and characteristics. These findings have demonstrated the crucial roles of SVs in regulating gene expression, driving trait innovation, facilitating domestication, making this an opportune time for a systematic review. We summarized the progress of SV-related studies in oil crops, focusing on the types of SVs and their mechanisms of occurrence, the strategies and methods for SV detection, and the SVs identified in oil crops such as rapeseed, soybean, peanut, and sesame. The various types of SVs, such as presence-absence variations (PAVs), copy number variations (CNVs), and homeologous exchanges (HEs), have been shown. Along with their genomic characterization, their roles in crop domestication and breeding, and regulatory impact on gene expression and agronomic traits have also been demonstrated. This review will provide an overview of the SV research process in oil crops, enabling researchers to quickly understand key information and apply this knowledge in future studies and crop breeding.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 240-246"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the combined effects of geographical origin, cultivar and crop season on the physicochemical characteristics of Moroccan olive oils produced in northern Morocco. A comparative study","authors":"Noura Issaoui ,&nbsp;Inmaculada Olivares ,&nbsp;Amar Habsaoui ,&nbsp;Mohamed Ebn Touhami ,&nbsp;Adil Khtira ,&nbsp;El Hassan Sakar ,&nbsp;Sebastián Sánchez","doi":"10.1016/j.ocsci.2024.11.001","DOIUrl":"10.1016/j.ocsci.2024.11.001","url":null,"abstract":"<div><div>The Tangier-Tetouan-Al Hoceima (TTA) region is one of the main olive oil producing regions in Morocco. Little is devoted to characterize olive oil physicochemical traits from TTA hence the originality of this study. This aimed at investigating variation in olive oil quality produced from three Moroccan cultivars ‘Moroccan Picholine’, ‘Menara’, and ‘Haouzia’ and their blends. Sampling was performed in five provinces fromTTA (Northern Morocco) during four consecutive crop-seasons (2018–2021) considering three extraction technologies (ET): traditional discontinuous press system (SP) and continuous extraction systems including decanter of three outlets (3O) and decanter of two outlets (2O). Physicochemical measurements consisted of routinely quality parameters namely free acidity (FA), peroxide value (PV), UV absorption parameters (<em>K</em>₂₃₂, <em>K</em>₂₇₀, and <em>ΔK</em>), chlorophylls (Chl) and carotenoids (Car) contents, total phenolic compounds (TPC) and oxidative stability (OS). Crop season showed its superiority impacts on <em>K</em>₂₃₂, OS, TPC, Chl, and OS. While cultivar was the main variability source in both PV and <em>K</em>₂₇₀, and FA was mainly determined by ET. Important variations (<em>p</em> ​&lt; ​0.05) were reported among crop seasons and locations due to pedoclimatic differences. ‘Menara’ and ‘Haouzia’ had higher pigments content, TPC, and OS, and the blends displayed low pigments concentration, TPC, and OS. Expectedly, continuous ET (2O and 3O) had the greatest values of pigments content, TPC, and OS as revealed by principal component analysis. Strong correlations were highlighted among basic quality parameters, TPC, pigments, and OS. Simple linear regression was used to describe the relationships between OS and TPC (R² ​= ​0.856) and OS regressed against Chl (R² ​= ​0.690) and Car (R² ​= ​0.760), while TPC were regressed on Chl (R² ​= ​0.670) and Car (R² ​= ​0.680) and finally Chl against Car (R² ​= ​0.931). In conclusion, compared to technological, genotypic, and geographic effects, climatic conditions were the main factor driving olive oil stability and associated phenolics and pigments; oil cultivar blend seems to have negative effects on pigments concentration and total phenolic compounds as well as oxidative stability.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 255-264"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of different grass species extracts as source of micronutrients in prawn Macrobrachium rosenbergii feeds","authors":"","doi":"10.1016/j.aaf.2023.01.010","DOIUrl":"10.1016/j.aaf.2023.01.010","url":null,"abstract":"<div><div>The present research is an attempt to screen five perennial indigenous grass species for their biochemical constituents seasonally for one year in order to determine the exact season for the harvest of the target species which will be used as feed ingredient. Among them, <em>Cynodon dactylon</em> has shown better result compared to other grass species and was hence incorporated as micronutrient in the formulated feed. <em>Macrobrachium rosenbergii</em> culture was carried out by feeding conventional and formulated feed (prepared from grass species) in tanks and ponds filled with spring water for 180 days in 2020 in the Eastern Ghats region of Koraput district, Odisha, India. Two treatments namely T₁ and T₂ having four replications each were supplied with commercial feed and formulated feed. Feeds were applied as per the body weight of prawns at 20% for the 1st month, 15% for the 2nd month, 10% for the 3rd and the 4th month, 5% for the 5th month and 2% till the end of the experiment. The water temperature, pH, dissolved oxygen, transparency, nitrate, phosphate, silicate, Chl <em>a</em> in the culture tanks and ponds were monitored fortnightly. ANOVA results between all the water parameters for both culture tanks and ponds showed significant variation (<em>P</em> &lt; 0.05). The survival rate (77.0% ± 0.89%), daily growth rate (0.233 ± 0.05) g/day, specific growth rate (4.657% ± 1.04%) per day and condition index (2.638 ± 0.05) were found to be highest in prawns fed formulated feed. The feed conversion ratio varied from (2.03 ± 0.05) to (2.68 ± 0.04). Benefit cost ratio of field culture were 1.46 with yield of 702 kg/ha in conventional feed and 1.75 with yield of 950 kg/ha in formulated feed respectively. The study proved grass extracts to be efficient micronutrients for prawn feed which can be easily adopted in prawn production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 937-947"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78346294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}