农学最新文献

{"title":"Impact of deacidification processes on the quality and oxidative stability of walnut oil","authors":"Xuan Ma ,&nbsp;Chongbo Huang ,&nbsp;Chang Zheng ,&nbsp;Fangyan Long ,&nbsp;Mandi Zhao ,&nbsp;Changsheng Liu","doi":"10.1016/j.ocsci.2024.10.001","DOIUrl":"10.1016/j.ocsci.2024.10.001","url":null,"abstract":"<div><div>In order to select an appropriate deacidification process and improve the quality of walnut oil, low-temperature cold-pressed crude walnut oil was used as raw material. Deacidified walnut oil was prepared using three deacidification processes: chemical deacidification (CD), adsorption deacidification (AD), and molecular distillation deacidification (MDD). The physicochemical properties, nutritional components, and <em>in vitro</em> antioxidant activities of the resulting deacidified walnut oils were comparatively analyzed. The results indicate that the fatty acid content in walnut oil exhibits fluctuating changes during the three different deacidification processes. The MDD shows a higher deacidification rate, reaching 94.06%, which is superior to the other two methods. Additionally, the AD retains more total phenols and tocopherols, with retention rates of 95.79% and 74.62%, respectively; whereas MDD is more effective at retaining phytosterols, achieving a retention rate of 98.09%. All these methods displayed positive impacts on the <em>in vitro</em> antioxidant capacity and oil stability of walnut oil, with ferric-reducing antioxidant power (FRAP) content and oxidative stability time were significantly reduced.whencompared to the untreated crude oil Among them, AD had the greatest impact on oxidative stability index (OSI), with its decreasing from 2.06 ​h to 0.82 ​h. Overall, compared to CD or MDD, the AD has best application prospects in preserving nutritional components.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 247-254"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of Recirculating Aquaculture System (RAS) with combination of clear water, probiotic and biofloc technology: A review","authors":"","doi":"10.1016/j.aaf.2023.07.006","DOIUrl":"10.1016/j.aaf.2023.07.006","url":null,"abstract":"<div><div>Recirculating Aquaculture System (RAS) is introduced in aquaculture farming industry to reduce water resource utilization, efficient the energy and land uses, and also help minimalize the water exchange. This system enables utilization of unsuitable land and promotes a sustainable environment in aquaculture industry. Furthermore, this technology has been established and proved efficient in monitoring the aquatic animal condition subsequently helps in maintaining the water quality and help remove solid particle wastes from the aquaculture treatment. As today, RAS has been developed with more effective technologies such as the use of UV irradiation, solid capture, protein skimmer and also provided with highly techno bio-filtration set. Basically, this system was applied for broodstock maturation, nursery phase, and grow-out production. In this review article, we provide an overview of RAS between the clear water, probiotic, and biofloc technology, and the advantages of its combination. Even though RAS and biofloc is two different parallel system, the application of the probiotic and biofloc in the semi-RAS application system is intense to be investigated. The synergistic effect of RAS using this combination towards high yield aquaculture production will be highlighted in this review paper. Expectantly this review paper will generate awareness and useful information on the RAS application in the aquaculture system operation with help in maximize the impact to the aquaculture yield production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 883-892"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73378125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofloc system supplemented by Pseudoalteromonas piscicida 1Ub protects the Pacific white shrimp Penaeus vannamei from Vibrio parahaemolyticus infection","authors":"","doi":"10.1016/j.aaf.2023.05.003","DOIUrl":"10.1016/j.aaf.2023.05.003","url":null,"abstract":"<div><div>This study aimed to evaluate the supplementation of the probiotic <em>Pseudoalteromonas piscicida</em> 1Ub to the biofloc system as an ecofriendly strategy for protecting white shrimp (<em>Penaeus vannamei</em>) from <em>Vibrio parahaemolyticus</em> infection. Shrimp with an average body weight of (0.50 ± 0.09) g were reared in 30 glass jars with a working volume of 2.5 L at a density of 20 ind/L. Shrimp were reared for 5 d for each treatment, which included the biofloc system without and with 10⁶ colony forming unit (CFU) per mL probiotic. The regular clear water system was used as control. All treatment groups were challenged with 10³, 10⁵, and 10⁷ CFU/mL <em>V. parahaemolyticus</em>. For the negative control, shrimp were reared without <em>V. parahaemolyticus</em>. The results showed that the density of <em>V. parahaemolyticus</em> cocultured with <em>P. piscicida</em> 1Ub decreased and the density of <em>V. parahaemolyticus</em> in rearing water and shrimp body in the probiotic-treated group was lower than that in the control group (<em>P</em> &lt; 0.05). The survival and immune response (total hemocyte count, phagocytic activity, respiratory burst, phenoloxidase, and superoxide dismutase) of shrimp in the probiotic group was higher than that in the positive control (<em>P</em> &lt; 0.05). Moreover, supplementing the biofloc system with the probiotic could protect shrimp hepatopancreas from damage caused by <em>V. parahaemolyticus</em>, regardless of bacterial density. Thus, the supplementation of the probiotic <em>P. piscicida</em> 1Ub in the biofloc system could significantly protect and increase the resistance of shrimp to <em>V. parahaemolyticus</em> infection.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 967-974"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74924612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug resistance mechanism of anti-angiogenesis therapy in tumor.","authors":"Xu Yan, Ying Guo, Dong-Lin Sun, Nan Wu, Yan Jin","doi":"10.16288/j.yczz.24-110","DOIUrl":"10.16288/j.yczz.24-110","url":null,"abstract":"<p><p>Angiogenesis refers to the process of forming a new network of blood vessels from existing ones through the migration, proliferation, and differentiation of endothelial cells. This process is crucial for the growth and spread of solid tumors, particularly once the tumor volume exceeds 2 mm³, as the newly formed vascular network provides essential oxygen, nutrients, and growth factors to the tumor. Anti-angiogenesis therapy has become one of the commonly used targeted treatments for cancer in clinical practice. Bevacizumab, the first anti-angiogenesis drug, has been widely applied in the treatment of various solid tumors. However, due to acquired resistance, its efficacy is typically sustained for only 1 to 2 years. Despite the relative genomic stability of endothelial cells, which makes resistance less likely, various types of resistance phenomena have been observed in clinical practice, indicating that resistance to anti-angiogenic therapy remains a challenging research area. This review focuses on the latest advances in the mechanisms of resistance to anti-angiogenic therapy in tumors and explores new prospects for anti-tumor angiogenesis treatment, in order to provide strong theoretical support and guidance for clinical practice.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"911-919"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-myc modulates the replication of RGNNV via glutamine-mediated ATP production in grouper fin cells","authors":"","doi":"10.1016/j.aaf.2023.05.005","DOIUrl":"10.1016/j.aaf.2023.05.005","url":null,"abstract":"<div><div><em>C-myc</em> is a proto-oncogene that plays an important role in a variety of diseases. There were a lot of research on the correlation between C-myc and human viruses. However, the study about <em>C-myc</em> related to aquatic species virus is very limited. In the present study, the qRT-PCR, cellular immunofluorescence and western blotting determination data reported that <em>C-myc</em> and glutaminase (<em>GLS</em>) genes were significantly upregulated when grouper fin cells (GF-1) were infected with red grouper nervous necrosis virus (RGNNV). After knocking down the <em>C-myc</em> gene, the mRNA and protein levels of <em>GLS</em>, capsid protein (<em>CP</em>) and RNA polymerase (<em>RdRp</em>) of RGNNV were significantly reduced in RGNNV-infected GF-1 cells and the overexpression of the <em>C-myc</em> gene remarkably promoted these genes, which indicated that the replication of the virus and <em>GLS</em> gene were positively regulated by <em>C-myc</em> in RGNNV-infected GF-1 cells. In addition, supplementation of exogenous ATP can partially restore viral replication when RGNNV-infected GF-1 cells were cultured in glutamine-free medium, which confirmed that the glutamine was decomposed into ATP to provide energy for viral replication. Further studies confirmed that overexpression of <em>C-myc</em> can increase the content of ATP in normal cells. To sum up, these data suggested that activation of <em>C-myc</em> gene affected viral replication by regulating <em>GLS</em> expression to drive glutamine dissolution.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 929-936"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82520737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking epigenetic breeding potential in tomato and potato","authors":"Pingxian Zhang,&nbsp;Yuehui He,&nbsp;Sanwen Huang","doi":"10.1007/s42994-024-00184-2","DOIUrl":"10.1007/s42994-024-00184-2","url":null,"abstract":"<div><p>Tomato (<i>Solanum lycopersicum</i>) and potato (<i>Solanum tuberosum</i>), two integral crops within the nightshade family, are crucial sources of nutrients and serve as staple foods worldwide. Molecular genetic studies have significantly advanced our understanding of their domestication, evolution, and the establishment of key agronomic traits. Recent studies have revealed that epigenetic modifications act as “molecular switches”, crucially regulating phenotypic variations essential for traits such as fruit ripening in tomatoes and tuberization in potatoes. This review summarizes the latest findings on the regulatory mechanisms of epigenetic modifications in these crops and discusses the integration of biotechnology and epigenomics to enhance breeding strategies. By highlighting the role of epigenetic control in augmenting crop yield and adaptation, we underscores its potential to address the challenges posed by a growing global population as well as changing climate.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 4","pages":"507 - 518"},"PeriodicalIF":4.6,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00184-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thiophanate-methyl and its major metabolite carbendazim weaken rhizobacteria-mediated defense responses in cucumbers against Fusarium wilt","authors":"Kai Cui,&nbsp;Xiaoming Xia,&nbsp;Youwei Wang,&nbsp;Yueli Zhang,&nbsp;Ying Zhang,&nbsp;Junli Cao,&nbsp;Jun Xu,&nbsp;Fengshou Dong,&nbsp;Xingang Liu,&nbsp;Xinglu Pan,&nbsp;Yongquan Zheng,&nbsp;Xiaohu Wu","doi":"10.1007/s42994-024-00181-5","DOIUrl":"10.1007/s42994-024-00181-5","url":null,"abstract":"<div><p>The effect of fungicides on the plant-rhizosphere microbiome is a subject of ongoing debate, but whether any alteration in the rhizosphere microbiome could affect plant health is an issue that has not been thoroughly investigated. To address this deficiency, we analyzed the rhizosphere microbiome of wilt disease—resistant and disease-susceptible cucumber cultivars to determine whether (and which) plant-associated microorganisms have a role in disease resistance. We further assessed whether the fungicides thiophanate-methyl and carbendazim affect the rhizosphere microbiome, which may contribute to the plant’s immune response. Based on results acquired with both radicle-inoculation and soil-inoculation methods, cultivars Longyuanxiuchun (LYXC) and Shuyan2 (SY2) were identified as being disease resistant, whereas Zhongnong6 (ZN6) and Zhongnong38 (ZN38) were susceptible. The microbiome structure differed substantially between the resistant and susceptible plants, with LYXC and SY2 each having a significantly greater Shannon index than Zhongnong38. These results revealed that the disease-resistant cucumber cultivars recruited more beneficial bacteria, i.e., <i>Bacillus</i>, in their rhizosphere soil; as such, <i>Bacillus</i> was identified as a keystone genus in the microbial co-occurrence network. Thus, the presence of <i>Bacillus</i> may help cucumbers defend against fungal pathogens within the rhizosphere. <i>Bacillus subtilis</i> strain LD15, which was isolated from LYXC rhizosphere soil, could suppress pathogen growth, in vitro, and reduce disease severity in pot assays. Moreover, evidence also confirmed the accumulation of LD1 in the rhizosphere soil of resistant cucumber cultivars. For LYXC, application of thiophanate-methyl or carbendazim altered the microbiome structure, decreased bacterial diversity, and reduced the abundance of <i>Bacillus</i> species. Finally, pot assays verified that fungicide application decreased the proportion of LD15 in rhizosphere soil. From a microbial perspective, thiophanate-methyl and carbendazim may weaken the rhizobacteria-mediated defense response of cucumbers against cucumber Fusarium wilt disease. Our findings reveal a role for the rhizosphere microbiome in protecting plants from pathogens and constitute a reference for assessing the ecotoxicological risk of pesticides to non-target soil microorganisms.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 4","pages":"417 - 430"},"PeriodicalIF":4.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00181-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: The RUBY reporter for visual selection in soybean genome editing","authors":"Li Chen,&nbsp;Yupeng Cai,&nbsp;Xiaoqian Liu,&nbsp;Weiwei Yao,&nbsp;Shuiqing Wu,&nbsp;Wensheng Hou","doi":"10.1007/s42994-024-00156-6","DOIUrl":"10.1007/s42994-024-00156-6","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 4","pages":"519 - 519"},"PeriodicalIF":4.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00156-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Processing pipelines and analytical methods for single-cell DNA methylation sequencing data.","authors":"Yan-Ni Wang, Jia Li","doi":"10.16288/j.yczz.24-154","DOIUrl":"https://doi.org/10.16288/j.yczz.24-154","url":null,"abstract":"<p><p>Single-cell DNA methylation sequencing technology has seen rapid advancements in recent years, playing a crucial role in uncovering cellular heterogeneity and the mechanisms of epigenetic regulation. As sequencing technologies have progressed, the quality and quantity of single-cell methylation data have also increased, making standardized preprocessing workflows and appropriate analysis methods essential for ensuring data comparability and result reliability. However, a comprehensive data analysis pipeline to guide researchers in mining existing data has yet to be established. This review systematically summarizes the preprocessing steps and analysis methods for single-cell methylation data, introduces relevant algorithms and tools, and explores the application prospects of single-cell methylation technology in neuroscience, hematopoietic differentiation, and cancer research. The aim is to provide guidance for researchers in data analysis and to promote the development and application of single-cell methylation sequencing technology.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 10","pages":"807-819"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational dissection of the regulatory mechanisms of aberrant metabolism in remodeling the microenvironment of breast cancer.","authors":"Yu-Xin Wan, Xin-Yu Zhu, Yu Zhao, Na Sun, Tian-Tong-Fei Jiang, Juan Xu","doi":"10.16288/j.yczz.24-167","DOIUrl":"https://doi.org/10.16288/j.yczz.24-167","url":null,"abstract":"<p><p>The composition of T cell subsets and tumor-specific T cell interactions within the tumor microenvironment (TME) contribute to the heterogeneity observed in breast cancer. Moreover, aberrant tumor metabolism is often intimately linked to dysregulated anti-tumor immune function of T cells. Identifying key metabolic genes that affect immune cell interactions thus holds promise for uncovering potential therapeutic targets in the treatment of breast cancer. This study leverages single-cell transcriptomic data from breast cancer to investigate tumor-specific T-cell subsets and their interacting subnetworks in the TME during cancer progression. We further assess the metabolic pathway activities of tumor-specifically activated T-cell subsets. The results reveal that metabolic pathways involved in insulin synthesis, secretion, degradation, as well as fructose catabolism, significantly influence multiple T cell interactions. By integrating the metabolic pathways that significantly up-regulate T cells in tumors and influence their interactions, we identify key abnormal metabolic genes associated with T-cell collaboration and further develop a breast cancer risk assessment model. Additionally, using gene expression profiles of prognosis-related genes significantly associated with aberrant metabolism and drug IC50 values, we predict targeted drugs, yielding potential candidates like GSK-J4 and PX-12. This study integrate the analysis of abnormal T-cell interactions and metabolic pathway abnormalities in the breast cancer TME, elucidating their roles in cancer progression and providing leads for novel breast cancer therapeutic strategies.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 10","pages":"871-885"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}