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Sturgeon and paddlefish: Review of research on broodstock and early life stage management 鲟鱼和桨鱼:鱼种和早期生命阶段管理研究综述
Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.04.001
{"title":"Sturgeon and paddlefish: Review of research on broodstock and early life stage management","authors":"","doi":"10.1016/j.aaf.2023.04.001","DOIUrl":"10.1016/j.aaf.2023.04.001","url":null,"abstract":"<div><div>Sturgeon aquaculture has taken a parallel yet unique path relative to other teleost rearing. One of the driving factors has been the fact that virtually all extant species are facing extinction; thus, laws concerning the transport and processing of sturgeon are stringent. As a result, most sturgeon companies vertically integrate the farming process from hatchery to harvest, in contrast to the production of other commercial fish species (e.g., trout, sea bass, salmon), where farmers tend to purchase fry from producers specialized only in the production of that life stage and sell product to unaffiliated fish processors. Sturgeon aquaculture is furthermore unique in the extremely high value of the main product (caviar). The secondary product (meat) also surpasses other finfish aquaculture products' average price per weight. These expensive outputs reveal the greatest challenge in production — an exceptionally long time to market from larvae to harvestable adults. For all their rigor and robustness as juveniles and adults, sturgeon species are highly fragile during larval stages. Due to the unique legal framework around sturgeon cultivation, aquaculturists often specialize in regional species, although some hybrids have achieved global popularity. This lends to a heterogenous swath of literature on best cultivation and management practices, especially at early growth stages. Nonetheless, the role of aquaculture as both a food source and restocking program to thwart further extinction of sturgeon species has hardly been fully tapped. In light of the many challenges, this review examines recent advances in sturgeon farming with an emphasis on broodstock and early life-stage management to summarize studies that can help researchers and farmers assess the industry's current state.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 871-882"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82357053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of structural parameters on the hydrodynamic performance of vertical curved V-Type otter board 结构参数对垂直曲面 V 型獭兔板水动力性能的影响
Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.02.004
{"title":"Effect of structural parameters on the hydrodynamic performance of vertical curved V-Type otter board","authors":"","doi":"10.1016/j.aaf.2023.02.004","DOIUrl":"10.1016/j.aaf.2023.02.004","url":null,"abstract":"<div><div>In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board, this paper based on the computational fluid dynamics method, a three-dimensional numerical model of vertical curved V-type otter board is established to calculate and analyze its hydrodynamic performance, and the validity of the numerical simulation results is verified by designing and constructing a scaled model for dynamic flume experiments. On this basis, the effects of different structural parameters, such as the deflector angle, camber, and spacing, on the hydrodynamic performance of the vertical curved V-Type otter board are investigated and the optimal structural design scheme is determined. The results demonstrate that single or synchronous changes of the angle, camber, and spacing of the two deflectors have varying degrees of impact on the hydrodynamic performance of the vertical curved V-Type otter board. To improve the expansion effect of the otter board, setting the angle of the deflector A to 40° and that of the deflector B to 30°, or setting the camber of deflectors A and B to 18%, or setting the spacing between deflectors A and B to 380 mm and that between the deflector and the main panel to 670 mm can increase the lift coefficient of the vertical curved V-Type otter board to its maximum value. Considering the overall performance of the otter board, when the angle of the deflector A is set to 25° and that of the deflector B is set to 30°, or the camber of the deflector A is set to 6% and that of the deflector B is set to 9%, or the spacing between deflectors A and B is set to 340 mm and that between the deflector and the main panel is set to 610 mm, the lift-to-drag ratio of the otter board can be increased to its maximum value.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 989-999"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90304361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural variations in oil crops: Types, and roles on domestication and breeding 油料作物的结构变化:类型以及对驯化和育种的作用
Oil Crop Science Pub Date : 2024-11-01 DOI: 10.1016/j.ocsci.2024.09.002
Xiaobo Cui, Miao Yao, Meili Xie, Ming Hu, Shengyi Liu, Lijiang Liu, Chaobo Tong
{"title":"Structural variations in oil crops: Types, and roles on domestication and breeding","authors":"Xiaobo Cui,&nbsp;Miao Yao,&nbsp;Meili Xie,&nbsp;Ming Hu,&nbsp;Shengyi Liu,&nbsp;Lijiang Liu,&nbsp;Chaobo Tong","doi":"10.1016/j.ocsci.2024.09.002","DOIUrl":"10.1016/j.ocsci.2024.09.002","url":null,"abstract":"<div><div>Structural variations (SVs), a newly discovered genetic variation, have gained increasing recognition for their importance, yet much about them remains unknown. With the completion of whole-genome sequencing projects in oil crops, more SVs have been identified, revealing their types, genomic distribution, and characteristics. These findings have demonstrated the crucial roles of SVs in regulating gene expression, driving trait innovation, facilitating domestication, making this an opportune time for a systematic review. We summarized the progress of SV-related studies in oil crops, focusing on the types of SVs and their mechanisms of occurrence, the strategies and methods for SV detection, and the SVs identified in oil crops such as rapeseed, soybean, peanut, and sesame. The various types of SVs, such as presence-absence variations (PAVs), copy number variations (CNVs), and homeologous exchanges (HEs), have been shown. Along with their genomic characterization, their roles in crop domestication and breeding, and regulatory impact on gene expression and agronomic traits have also been demonstrated. This review will provide an overview of the SV research process in oil crops, enabling researchers to quickly understand key information and apply this knowledge in future studies and crop breeding.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 240-246"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New insights into the combined effects of geographical origin, cultivar and crop season on the physicochemical characteristics of Moroccan olive oils produced in northern Morocco. A comparative study 地理来源、栽培品种和作物季节对摩洛哥北部生产的摩洛哥橄榄油理化特性的综合影响的新见解。比较研究
Oil Crop Science Pub Date : 2024-11-01 DOI: 10.1016/j.ocsci.2024.11.001
Noura Issaoui , Inmaculada Olivares , Amar Habsaoui , Mohamed Ebn Touhami , Adil Khtira , El Hassan Sakar , Sebastián Sánchez
{"title":"New insights into the combined effects of geographical origin, cultivar and crop season on the physicochemical characteristics of Moroccan olive oils produced in northern Morocco. A comparative study","authors":"Noura Issaoui ,&nbsp;Inmaculada Olivares ,&nbsp;Amar Habsaoui ,&nbsp;Mohamed Ebn Touhami ,&nbsp;Adil Khtira ,&nbsp;El Hassan Sakar ,&nbsp;Sebastián Sánchez","doi":"10.1016/j.ocsci.2024.11.001","DOIUrl":"10.1016/j.ocsci.2024.11.001","url":null,"abstract":"<div><div>The Tangier-Tetouan-Al Hoceima (TTA) region is one of the main olive oil producing regions in Morocco. Little is devoted to characterize olive oil physicochemical traits from TTA hence the originality of this study. This aimed at investigating variation in olive oil quality produced from three Moroccan cultivars ‘Moroccan Picholine’, ‘Menara’, and ‘Haouzia’ and their blends. Sampling was performed in five provinces fromTTA (Northern Morocco) during four consecutive crop-seasons (2018–2021) considering three extraction technologies (ET): traditional discontinuous press system (SP) and continuous extraction systems including decanter of three outlets (3O) and decanter of two outlets (2O). Physicochemical measurements consisted of routinely quality parameters namely free acidity (FA), peroxide value (PV), UV absorption parameters (<em>K</em><sub>232</sub>, <em>K</em><sub>270</sub>, and <em>ΔK</em>), chlorophylls (Chl) and carotenoids (Car) contents, total phenolic compounds (TPC) and oxidative stability (OS). Crop season showed its superiority impacts on <em>K</em><sub>232</sub>, OS, TPC, Chl, and OS. While cultivar was the main variability source in both PV and <em>K</em><sub>270</sub>, and FA was mainly determined by ET. Important variations (<em>p</em> ​&lt; ​0.05) were reported among crop seasons and locations due to pedoclimatic differences. ‘Menara’ and ‘Haouzia’ had higher pigments content, TPC, and OS, and the blends displayed low pigments concentration, TPC, and OS. Expectedly, continuous ET (2O and 3O) had the greatest values of pigments content, TPC, and OS as revealed by principal component analysis. Strong correlations were highlighted among basic quality parameters, TPC, pigments, and OS. Simple linear regression was used to describe the relationships between OS and TPC (R<sup>2</sup> ​= ​0.856) and OS regressed against Chl (R<sup>2</sup> ​= ​0.690) and Car (R<sup>2</sup> ​= ​0.760), while TPC were regressed on Chl (R<sup>2</sup> ​= ​0.670) and Car (R<sup>2</sup> ​= ​0.680) and finally Chl against Car (R<sup>2</sup> ​= ​0.931). In conclusion, compared to technological, genotypic, and geographic effects, climatic conditions were the main factor driving olive oil stability and associated phenolics and pigments; oil cultivar blend seems to have negative effects on pigments concentration and total phenolic compounds as well as oxidative stability.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 255-264"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of different grass species extracts as source of micronutrients in prawn Macrobrachium rosenbergii feeds 不同草种提取物作为对虾饲料中微量元素来源的评估
Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.01.010
{"title":"Assessment of different grass species extracts as source of micronutrients in prawn Macrobrachium rosenbergii feeds","authors":"","doi":"10.1016/j.aaf.2023.01.010","DOIUrl":"10.1016/j.aaf.2023.01.010","url":null,"abstract":"<div><div>The present research is an attempt to screen five perennial indigenous grass species for their biochemical constituents seasonally for one year in order to determine the exact season for the harvest of the target species which will be used as feed ingredient. Among them, <em>Cynodon dactylon</em> has shown better result compared to other grass species and was hence incorporated as micronutrient in the formulated feed. <em>Macrobrachium rosenbergii</em> culture was carried out by feeding conventional and formulated feed (prepared from grass species) in tanks and ponds filled with spring water for 180 days in 2020 in the Eastern Ghats region of Koraput district, Odisha, India. Two treatments namely T<sub>1</sub> and T<sub>2</sub> having four replications each were supplied with commercial feed and formulated feed. Feeds were applied as per the body weight of prawns at 20% for the 1st month, 15% for the 2nd month, 10% for the 3rd and the 4th month, 5% for the 5th month and 2% till the end of the experiment. The water temperature, pH, dissolved oxygen, transparency, nitrate, phosphate, silicate, Chl <em>a</em> in the culture tanks and ponds were monitored fortnightly. ANOVA results between all the water parameters for both culture tanks and ponds showed significant variation (<em>P</em> &lt; 0.05). The survival rate (77.0% ± 0.89%), daily growth rate (0.233 ± 0.05) g/day, specific growth rate (4.657% ± 1.04%) per day and condition index (2.638 ± 0.05) were found to be highest in prawns fed formulated feed. The feed conversion ratio varied from (2.03 ± 0.05) to (2.68 ± 0.04). Benefit cost ratio of field culture were 1.46 with yield of 702 kg/ha in conventional feed and 1.75 with yield of 950 kg/ha in formulated feed respectively. The study proved grass extracts to be efficient micronutrients for prawn feed which can be easily adopted in prawn production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 937-947"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78346294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Progress on the mining of functional genes of Lonicera japonica. 忍冬功能基因的挖掘进展。
遗传 Pub Date : 2024-11-01 DOI: 10.16288/j.yczz.24-190
Nan Sun, Lu-Yao Huang, Sheng Yang, Jia Li, Cong-Zhe Hou, Zhen-Hua Liu
{"title":"Progress on the mining of functional genes of <i>Lonicera japonica</i>.","authors":"Nan Sun, Lu-Yao Huang, Sheng Yang, Jia Li, Cong-Zhe Hou, Zhen-Hua Liu","doi":"10.16288/j.yczz.24-190","DOIUrl":"10.16288/j.yczz.24-190","url":null,"abstract":"<p><p><i>Lonicera japonica</i> Thunb. is a semi-evergreen climbing shrub belonging to the Caprifoliaceae family, whose dried flower buds or flowers on the verge of blooming are known as Jin Yin Hua in traditional Chinese medicine. This plant is not only a high-value and widely used medicinal material but also possesses characteristics that make it suitable for both medicinal and culinary purposes. Currently, there is a robust market demand for Jin Yin Hua, yet the breeding technology for new varieties of <i>Lonicera japonica</i> lags behind, necessitating the integration of modern breeding techniques. With the advancement of genomics in <i>Lonicera japonica</i>, an increasing number of functional genes have been identified, amassing a rich reservoir of genetic resources for molecular breeding of this species. In this review, we summarize the progress in <i>Lonicera japonica</i> genomics, functional gene mining, and the establishment of genetic transformation systems. In light of the existing challenges and deficiencies in the research of functional genes and quality breeding of <i>Lonicera japonica</i>, it is imperative to establish a germplasm resource bank, a mutant library, and an efficient genetic transformation system for this plant. Intensive research into the mining and identification of functional genes should be conducted, and molecular markers closely linked to the functional genes of <i>Lonicera japonica</i> should be developed. This will lay a foundational basis for the identification and cultivation of breakthrough varieties with superior qualities in <i>Lonicera japonica</i>.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"920-936"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive analysis and selection of high oleic peanut varieties in China: A study on agronomic, yield, and quality traits 中国高油酸花生品种的综合分析与筛选:农艺、产量和品质性状研究
Oil Crop Science Pub Date : 2024-11-01 DOI: 10.1016/j.ocsci.2024.09.001
Fangping Gong , Zhuo Li , Xiaojian Sun, Yi Fan, Yinghui Liu, Yurong Dang, Hao Li, Xingguo Zhang, Xingli Ma, Zhongfeng Li, Kai Zhao, Dongmei Yin
{"title":"Comprehensive analysis and selection of high oleic peanut varieties in China: A study on agronomic, yield, and quality traits","authors":"Fangping Gong ,&nbsp;Zhuo Li ,&nbsp;Xiaojian Sun,&nbsp;Yi Fan,&nbsp;Yinghui Liu,&nbsp;Yurong Dang,&nbsp;Hao Li,&nbsp;Xingguo Zhang,&nbsp;Xingli Ma,&nbsp;Zhongfeng Li,&nbsp;Kai Zhao,&nbsp;Dongmei Yin","doi":"10.1016/j.ocsci.2024.09.001","DOIUrl":"10.1016/j.ocsci.2024.09.001","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) is an important oil crop. Oleic acid is a major factor that determines the quality of peanuts. Therefore, the high oleic and high oleic to linoleic acid ratio are the target traits in an advanced peanut breeding program. This study provided an extensive evaluation of the genetic and physical characteristics as well as disease resistance of 220 high oleic peanut varieties in China. Notably, these varieties clustered into five major categories based on their traits. A majority of these varieties have been bred using interspecific hybridization or selected from mutants of self-crossed parents, with the main parent varieties being Kaixuan 016 and CTWE. Analysis of disease resistance showed that most high oleic peanut varieties could resist two or three diseases. However, those varieties with resistance to multiple diseases were relatively scarce. Moreover, some high oleic peanut varieties showed no disease resistance or inadequate testing. The results further indicate that the genetic basis for high oleic peanut breeding is insufficient, highlighting the need for its further development. Importantly, our findings lay a critical foundation for future high oleic peanut breeding and promote better understanding of the genetic and trait diversity offered by these varieties.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 265-274"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Used vs. new: Does it have consequences for the performance of fishing gear? 新旧对比:这对渔具性能有影响吗?
Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.03.003
{"title":"Used vs. new: Does it have consequences for the performance of fishing gear?","authors":"","doi":"10.1016/j.aaf.2023.03.003","DOIUrl":"10.1016/j.aaf.2023.03.003","url":null,"abstract":"<div><div>Sea trials to test size selectivity equipment in trawls are often limited in time because they are costly, and the results can be influenced by multiple factors that are often overlooked. In the Barents Sea gadoid trawl fishery, the use of a size sorting grid in front of the codend is compulsory. The flexigrid, a netting section containing two flexible grids, is the most widely used grid section in this fishery. However, earlier selectivity studies with this device have shown inconclusive results. It has been speculated that the differences observed resulted from the difference in age and usage of the grid sections in the studies compared. To reveal whether potential changes in the device construction over time can lead to differences in size selection properties, we performed comparative fishing trials where we compared a brand new flexigrid section and a well-used flexigrid section used continuously by a commercial trawler for approximately four years. The results showed that the new flexigrid released significantly more cod below ∼60 cm than the used flexigrid. However, when the grids were fished with a subsequent diamond mesh codend, there was no difference in the overall selectivity of the two gears, meaning that the size selectivity in the codend compensates for the potential reduction in selectivity performance of the grids. This study shows the importance of considering the age and earlier use of size selection devices like sorting grids before they are compared with other devices, as their size selection properties can change significantly over time and with use.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 981-988"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74943537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimal effort, fish farming, and marine reserve in fisheries management 渔业管理中的最佳努力、鱼类养殖和海洋保护区
Aquaculture and Fisheries Pub Date : 2024-11-01 DOI: 10.1016/j.aaf.2023.03.001
{"title":"Optimal effort, fish farming, and marine reserve in fisheries management","authors":"","doi":"10.1016/j.aaf.2023.03.001","DOIUrl":"10.1016/j.aaf.2023.03.001","url":null,"abstract":"<div><div>In this paper, we develop a bioeconomic model to determine an optimal wild catch harvest and freshwater commercial aquaculture production in a lake that uses cages or floating rafts. Although off-bottom aquaculture activities have less impact on the environment compared to on-bottom aquaculture, it has negative externalities. It takes away the fishing area and hence creates pressure in the open-access fishing ground. It also changes the nutrient and ecological composition of the environment. We recommend the implementation of a reserved area to minimize the impact of fishing and aquaculture activities. The reserve also helps in improving the habitat and restocking the fish population which is declining rapidly. We determine the optimal sizes of the aquaculture and reserve and the optimal effort level that maximizes social welfare. We illustrate our findings using numerical simulations and perform an analysis of the optimal solutions with respect to biological and economic parameters involved in the model.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 975-980"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84875970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual-localization signals enhance mitochondrial targeted presentation of engineered proteins. 双定位信号增强了线粒体对工程蛋白质的定向呈现。
遗传 Pub Date : 2024-11-01 DOI: 10.16288/j.yczz.24-171
Bing-Qian Zhou, Shang-Pu Li, Xu Wang, Xiang-Yu Meng, Jing-Rong Deng, Jin-Liang Xing, Jian-Gang Wang, Kun Xu
{"title":"Dual-localization signals enhance mitochondrial targeted presentation of engineered proteins.","authors":"Bing-Qian Zhou, Shang-Pu Li, Xu Wang, Xiang-Yu Meng, Jing-Rong Deng, Jin-Liang Xing, Jian-Gang Wang, Kun Xu","doi":"10.16288/j.yczz.24-171","DOIUrl":"10.16288/j.yczz.24-171","url":null,"abstract":"<p><p>Effective delivery of engineered proteins into mitochondria is of great significance for developing efficient mitochondrial DNA editing tools and realizing accurate treatment of mitochondrial diseases. Here, the candidate genes, <i>eGFP</i> and <i>Cas9</i>, were engineered with different mitochondrial localization signal (MLS) sequences introduced at their up- or/and down-streams. The corresponding expression vectors for the engineered proteins were constructed respectively, and HEK293T cells were transfected with these vectors. The fluorescence colocalization and Western blotting assays were used to analyze the mitochondrial targeting presentation effect of different engineered proteins. The results demonstrated that the daul-MLS modification of the eGFP and Cas9 proteins significantly improved the efficiency of mitochondrial targeted presentation, compared with the engineered proteins with single MLS added. Hence, it is speculated that dual MLS strategy can enhance the mitochondrial targeting of engineered proteins, which lays a theoretical foundation for the future development of efficient mitochondrial DNA editing tools.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"937-946"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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