农学最新文献

{"title":"A compact underwater stereo vision system for measuring fish","authors":"","doi":"10.1016/j.aaf.2023.03.006","DOIUrl":"10.1016/j.aaf.2023.03.006","url":null,"abstract":"<div><div>In the context of aquaculture, fish length is a key parameter to assess fish stocks, essential for feeding regime and contributes for decisions at several production levels. However, taking length measurements is a cumbersome task that, when applied to living individuals, can induce considerable stress, increasing the risk of damage or hindering their growth. Computer vision is one of the most used non-contact tools to overcome this issue, being fast, consistent and repeatable. However, its use in aquatic environments is limited by the high cost, the difficulty of calibrating the system in underwater conditions and the complexity of implementation. This paper proposes a low-cost easy-to-use vision system that can take measurements on live fish in aquatic conditions, without the need for a special calibration or a demanding in-water calibration service. The present work implemented a compact stereo vision system and developed a method that estimates the correct length of fish, based on the variation of the angle of incidence of the light rays in the water. Given some structural conditions such as a short baseline, the system is able to measure fish with an error of less than 1%. The short baseline allows to have a compact system and reduces the effect of water refraction on the 3D reconstruction. A set of experiments were performed with real fish, working robustly for a set of orientations of the fish (even when the caudal fin and snout are on different distances to the cameras).</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 1000-1006"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75300570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sturgeon and paddlefish: Review of research on broodstock and early life stage management","authors":"","doi":"10.1016/j.aaf.2023.04.001","DOIUrl":"10.1016/j.aaf.2023.04.001","url":null,"abstract":"<div><div>Sturgeon aquaculture has taken a parallel yet unique path relative to other teleost rearing. One of the driving factors has been the fact that virtually all extant species are facing extinction; thus, laws concerning the transport and processing of sturgeon are stringent. As a result, most sturgeon companies vertically integrate the farming process from hatchery to harvest, in contrast to the production of other commercial fish species (e.g., trout, sea bass, salmon), where farmers tend to purchase fry from producers specialized only in the production of that life stage and sell product to unaffiliated fish processors. Sturgeon aquaculture is furthermore unique in the extremely high value of the main product (caviar). The secondary product (meat) also surpasses other finfish aquaculture products' average price per weight. These expensive outputs reveal the greatest challenge in production — an exceptionally long time to market from larvae to harvestable adults. For all their rigor and robustness as juveniles and adults, sturgeon species are highly fragile during larval stages. Due to the unique legal framework around sturgeon cultivation, aquaculturists often specialize in regional species, although some hybrids have achieved global popularity. This lends to a heterogenous swath of literature on best cultivation and management practices, especially at early growth stages. Nonetheless, the role of aquaculture as both a food source and restocking program to thwart further extinction of sturgeon species has hardly been fully tapped. In light of the many challenges, this review examines recent advances in sturgeon farming with an emphasis on broodstock and early life-stage management to summarize studies that can help researchers and farmers assess the industry's current state.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 871-882"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82357053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of structural parameters on the hydrodynamic performance of vertical curved V-Type otter board","authors":"","doi":"10.1016/j.aaf.2023.02.004","DOIUrl":"10.1016/j.aaf.2023.02.004","url":null,"abstract":"<div><div>In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board, this paper based on the computational fluid dynamics method, a three-dimensional numerical model of vertical curved V-type otter board is established to calculate and analyze its hydrodynamic performance, and the validity of the numerical simulation results is verified by designing and constructing a scaled model for dynamic flume experiments. On this basis, the effects of different structural parameters, such as the deflector angle, camber, and spacing, on the hydrodynamic performance of the vertical curved V-Type otter board are investigated and the optimal structural design scheme is determined. The results demonstrate that single or synchronous changes of the angle, camber, and spacing of the two deflectors have varying degrees of impact on the hydrodynamic performance of the vertical curved V-Type otter board. To improve the expansion effect of the otter board, setting the angle of the deflector A to 40° and that of the deflector B to 30°, or setting the camber of deflectors A and B to 18%, or setting the spacing between deflectors A and B to 380 mm and that between the deflector and the main panel to 670 mm can increase the lift coefficient of the vertical curved V-Type otter board to its maximum value. Considering the overall performance of the otter board, when the angle of the deflector A is set to 25° and that of the deflector B is set to 30°, or the camber of the deflector A is set to 6% and that of the deflector B is set to 9%, or the spacing between deflectors A and B is set to 340 mm and that between the deflector and the main panel is set to 610 mm, the lift-to-drag ratio of the otter board can be increased to its maximum value.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 989-999"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90304361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of different grass species extracts as source of micronutrients in prawn Macrobrachium rosenbergii feeds","authors":"","doi":"10.1016/j.aaf.2023.01.010","DOIUrl":"10.1016/j.aaf.2023.01.010","url":null,"abstract":"<div><div>The present research is an attempt to screen five perennial indigenous grass species for their biochemical constituents seasonally for one year in order to determine the exact season for the harvest of the target species which will be used as feed ingredient. Among them, <em>Cynodon dactylon</em> has shown better result compared to other grass species and was hence incorporated as micronutrient in the formulated feed. <em>Macrobrachium rosenbergii</em> culture was carried out by feeding conventional and formulated feed (prepared from grass species) in tanks and ponds filled with spring water for 180 days in 2020 in the Eastern Ghats region of Koraput district, Odisha, India. Two treatments namely T₁ and T₂ having four replications each were supplied with commercial feed and formulated feed. Feeds were applied as per the body weight of prawns at 20% for the 1st month, 15% for the 2nd month, 10% for the 3rd and the 4th month, 5% for the 5th month and 2% till the end of the experiment. The water temperature, pH, dissolved oxygen, transparency, nitrate, phosphate, silicate, Chl <em>a</em> in the culture tanks and ponds were monitored fortnightly. ANOVA results between all the water parameters for both culture tanks and ponds showed significant variation (<em>P</em> &lt; 0.05). The survival rate (77.0% ± 0.89%), daily growth rate (0.233 ± 0.05) g/day, specific growth rate (4.657% ± 1.04%) per day and condition index (2.638 ± 0.05) were found to be highest in prawns fed formulated feed. The feed conversion ratio varied from (2.03 ± 0.05) to (2.68 ± 0.04). Benefit cost ratio of field culture were 1.46 with yield of 702 kg/ha in conventional feed and 1.75 with yield of 950 kg/ha in formulated feed respectively. The study proved grass extracts to be efficient micronutrients for prawn feed which can be easily adopted in prawn production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 937-947"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78346294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on the mining of functional genes of <i>Lonicera japonica</i>.","authors":"Nan Sun, Lu-Yao Huang, Sheng Yang, Jia Li, Cong-Zhe Hou, Zhen-Hua Liu","doi":"10.16288/j.yczz.24-190","DOIUrl":"https://doi.org/10.16288/j.yczz.24-190","url":null,"abstract":"<p><p><i>Lonicera japonica</i> Thunb. is a semi-evergreen climbing shrub belonging to the Caprifoliaceae family, whose dried flower buds or flowers on the verge of blooming are known as Jin Yin Hua in traditional Chinese medicine. This plant is not only a high-value and widely used medicinal material but also possesses characteristics that make it suitable for both medicinal and culinary purposes. Currently, there is a robust market demand for Jin Yin Hua, yet the breeding technology for new varieties of <i>Lonicera japonica</i> lags behind, necessitating the integration of modern breeding techniques. With the advancement of genomics in <i>Lonicera japonica</i>, an increasing number of functional genes have been identified, amassing a rich reservoir of genetic resources for molecular breeding of this species. In this review, we summarize the progress in <i>Lonicera japonica</i> genomics, functional gene mining, and the establishment of genetic transformation systems. In light of the existing challenges and deficiencies in the research of functional genes and quality breeding of <i>Lonicera japonica</i>, it is imperative to establish a germplasm resource bank, a mutant library, and an efficient genetic transformation system for this plant. Intensive research into the mining and identification of functional genes should be conducted, and molecular markers closely linked to the functional genes of <i>Lonicera japonica</i> should be developed. This will lay a foundational basis for the identification and cultivation of breakthrough varieties with superior qualities in <i>Lonicera japonica</i>.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"920-936"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Used vs. new: Does it have consequences for the performance of fishing gear?","authors":"","doi":"10.1016/j.aaf.2023.03.003","DOIUrl":"10.1016/j.aaf.2023.03.003","url":null,"abstract":"<div><div>Sea trials to test size selectivity equipment in trawls are often limited in time because they are costly, and the results can be influenced by multiple factors that are often overlooked. In the Barents Sea gadoid trawl fishery, the use of a size sorting grid in front of the codend is compulsory. The flexigrid, a netting section containing two flexible grids, is the most widely used grid section in this fishery. However, earlier selectivity studies with this device have shown inconclusive results. It has been speculated that the differences observed resulted from the difference in age and usage of the grid sections in the studies compared. To reveal whether potential changes in the device construction over time can lead to differences in size selection properties, we performed comparative fishing trials where we compared a brand new flexigrid section and a well-used flexigrid section used continuously by a commercial trawler for approximately four years. The results showed that the new flexigrid released significantly more cod below ∼60 cm than the used flexigrid. However, when the grids were fished with a subsequent diamond mesh codend, there was no difference in the overall selectivity of the two gears, meaning that the size selectivity in the codend compensates for the potential reduction in selectivity performance of the grids. This study shows the importance of considering the age and earlier use of size selection devices like sorting grids before they are compared with other devices, as their size selection properties can change significantly over time and with use.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 981-988"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74943537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal effort, fish farming, and marine reserve in fisheries management","authors":"","doi":"10.1016/j.aaf.2023.03.001","DOIUrl":"10.1016/j.aaf.2023.03.001","url":null,"abstract":"<div><div>In this paper, we develop a bioeconomic model to determine an optimal wild catch harvest and freshwater commercial aquaculture production in a lake that uses cages or floating rafts. Although off-bottom aquaculture activities have less impact on the environment compared to on-bottom aquaculture, it has negative externalities. It takes away the fishing area and hence creates pressure in the open-access fishing ground. It also changes the nutrient and ecological composition of the environment. We recommend the implementation of a reserved area to minimize the impact of fishing and aquaculture activities. The reserve also helps in improving the habitat and restocking the fish population which is declining rapidly. We determine the optimal sizes of the aquaculture and reserve and the optimal effort level that maximizes social welfare. We illustrate our findings using numerical simulations and perform an analysis of the optimal solutions with respect to biological and economic parameters involved in the model.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 975-980"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84875970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-localization signals enhance mitochondrial targeted presentation of engineered proteins.","authors":"Bing-Qian Zhou, Shang-Pu Li, Xu Wang, Xiang-Yu Meng, Jing-Rong Deng, Jin-Liang Xing, Jian-Gang Wang, Kun Xu","doi":"10.16288/j.yczz.24-171","DOIUrl":"https://doi.org/10.16288/j.yczz.24-171","url":null,"abstract":"<p><p>Effective delivery of engineered proteins into mitochondria is of great significance for developing efficient mitochondrial DNA editing tools and realizing accurate treatment of mitochondrial diseases. Here, the candidate genes, <i>eGFP</i> and <i>Cas9</i>, were engineered with different mitochondrial localization signal (MLS) sequences introduced at their up- or/and down-streams. The corresponding expression vectors for the engineered proteins were constructed respectively, and HEK293T cells were transfected with these vectors. The fluorescence colocalization and Western blotting assays were used to analyze the mitochondrial targeting presentation effect of different engineered proteins. The results demonstrated that the daul-MLS modification of the eGFP and Cas9 proteins significantly improved the efficiency of mitochondrial targeted presentation, compared with the engineered proteins with single MLS added. Hence, it is speculated that dual MLS strategy can enhance the mitochondrial targeting of engineered proteins, which lays a theoretical foundation for the future development of efficient mitochondrial DNA editing tools.</p>","PeriodicalId":35536,"journal":{"name":"遗传","volume":"46 11","pages":"937-946"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of Recirculating Aquaculture System (RAS) with combination of clear water, probiotic and biofloc technology: A review","authors":"","doi":"10.1016/j.aaf.2023.07.006","DOIUrl":"10.1016/j.aaf.2023.07.006","url":null,"abstract":"<div><div>Recirculating Aquaculture System (RAS) is introduced in aquaculture farming industry to reduce water resource utilization, efficient the energy and land uses, and also help minimalize the water exchange. This system enables utilization of unsuitable land and promotes a sustainable environment in aquaculture industry. Furthermore, this technology has been established and proved efficient in monitoring the aquatic animal condition subsequently helps in maintaining the water quality and help remove solid particle wastes from the aquaculture treatment. As today, RAS has been developed with more effective technologies such as the use of UV irradiation, solid capture, protein skimmer and also provided with highly techno bio-filtration set. Basically, this system was applied for broodstock maturation, nursery phase, and grow-out production. In this review article, we provide an overview of RAS between the clear water, probiotic, and biofloc technology, and the advantages of its combination. Even though RAS and biofloc is two different parallel system, the application of the probiotic and biofloc in the semi-RAS application system is intense to be investigated. The synergistic effect of RAS using this combination towards high yield aquaculture production will be highlighted in this review paper. Expectantly this review paper will generate awareness and useful information on the RAS application in the aquaculture system operation with help in maximize the impact to the aquaculture yield production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 883-892"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73378125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofloc system supplemented by Pseudoalteromonas piscicida 1Ub protects the Pacific white shrimp Penaeus vannamei from Vibrio parahaemolyticus infection","authors":"","doi":"10.1016/j.aaf.2023.05.003","DOIUrl":"10.1016/j.aaf.2023.05.003","url":null,"abstract":"<div><div>This study aimed to evaluate the supplementation of the probiotic <em>Pseudoalteromonas piscicida</em> 1Ub to the biofloc system as an ecofriendly strategy for protecting white shrimp (<em>Penaeus vannamei</em>) from <em>Vibrio parahaemolyticus</em> infection. Shrimp with an average body weight of (0.50 ± 0.09) g were reared in 30 glass jars with a working volume of 2.5 L at a density of 20 ind/L. Shrimp were reared for 5 d for each treatment, which included the biofloc system without and with 10⁶ colony forming unit (CFU) per mL probiotic. The regular clear water system was used as control. All treatment groups were challenged with 10³, 10⁵, and 10⁷ CFU/mL <em>V. parahaemolyticus</em>. For the negative control, shrimp were reared without <em>V. parahaemolyticus</em>. The results showed that the density of <em>V. parahaemolyticus</em> cocultured with <em>P. piscicida</em> 1Ub decreased and the density of <em>V. parahaemolyticus</em> in rearing water and shrimp body in the probiotic-treated group was lower than that in the control group (<em>P</em> &lt; 0.05). The survival and immune response (total hemocyte count, phagocytic activity, respiratory burst, phenoloxidase, and superoxide dismutase) of shrimp in the probiotic group was higher than that in the positive control (<em>P</em> &lt; 0.05). Moreover, supplementing the biofloc system with the probiotic could protect shrimp hepatopancreas from damage caused by <em>V. parahaemolyticus</em>, regardless of bacterial density. Thus, the supplementation of the probiotic <em>P. piscicida</em> 1Ub in the biofloc system could significantly protect and increase the resistance of shrimp to <em>V. parahaemolyticus</em> infection.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"9 6","pages":"Pages 967-974"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74924612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}