生命科学最新文献

{"title":"Genomics control of biostimulant-induced stress tolerance and crop yield enhancement","authors":"Tsanko Gechev","doi":"10.1111/tpj.70382","DOIUrl":"https://doi.org/10.1111/tpj.70382","url":null,"abstract":"<p>Biostimulants are changing modern agriculture, as they have the potential to secure healthy and sustainable food production while preserving the environment. They have two main biological effects: growth promotion and stress protection. Both effects can lead to enhancement of the yield and improvement of the marketable grade of the produce in crops, without compromising crop quality. Their use increased exponentially in the past decade, as they are highly efficient, ecologically friendly (non-toxic, biodegradable), and applicable to all major crops. While exponential data on the physiological mechanisms of stress protection is accumulating in recent years, the information as to how biostimulants act at the molecular level is still rather limited. Here we review the growing evidence of the biostimulants role in stress protection and yield enhancement of crops, as well as the recent transcriptomic and metabolomic data, which indicate biostimulants' molecular mode of action. In particular, we outline the role of genes encoding signaling components, plant hormones (abscisic acid, brassinosteroids, and ethylene), genes encoding transcription factors from ERF, WRKY, NAC, and MYB families, and genes related to growth, photosynthesis, and stress response. Finally, we describe strategies to study the genetic and genomics control of biostimulants mode of action, with foci on stress tolerance and yield enhancement. In Arabidopsis, established systems for biostimulants-induced protection against drought and oxidative stress will allow both forward and reverse genetics approaches to identify key genes from the biostimulants network. Mutations in such genes compromise the stress-protective effect of biostimulants. In major crops such as pepper and tomato, large Genome Wide Association Studies (GWAS) panels can be utilized to study crops responses to biostimulants in terms of drought tolerance, fruit qualities, and yield in order to pinpoint genes controlling biostimulants-induced stress protection and yield enhancement. The combination of these approaches allows identification and verification of important genes involved in the pathways of biostimulant-induced stress protection and yield enhancement, as well as deciphering parts of the intricate biostimulant-signaling network.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two alternatively spliced variants of ZmHSF12 regulate the balance of plant growth and heat tolerance in maize and Arabidopsis","authors":"Junlong Qi,&nbsp;Chunxia Zhang,&nbsp;Longhui Yang,&nbsp;Ying Liu,&nbsp;Guoji Wang,&nbsp;Shixiao Li,&nbsp;Lynnette M. A. Dirk,&nbsp;A. Bruce Downie,&nbsp;Tianyong Zhao","doi":"10.1111/tpj.70372","DOIUrl":"https://doi.org/10.1111/tpj.70372","url":null,"abstract":"<div>\u0000 \u0000 <p>Heat shock factors (HSFs) are pivotal in regulating plant heat tolerance; however, the mechanisms HSFs employ in regulating transcription to maintain a balance of plant growth and heat tolerance are poorly understood. This study reports that two maize <i>HSF12</i> knockout lines are more sensitive to heat stress. <i>ZmHSF12</i> encodes two alternative spliced transcripts: <i>ZmHSF12-1</i> and <i>ZmHSF12-2</i>; overexpression of <i>ZmHSF12-2</i> enhances, whereas overexpression of <i>ZmHSF12</i>-<i>1</i> decreases plant heat tolerance, indicating the distinct functions of these two transcripts in plant heat stress response. In addition, ZmHSF12-2 upregulates <i>RAFFINOSE SYNTHASE</i> (<i>ZmRAFS</i>) and <i>CYTOKININ OXIDASE</i> (<i>ZmCKO2</i>) gene expression, controlling raffinose and cytokinin concentration in the cell, enhancing plant heat tolerance and inhibiting plant growth. ZmHSF12-1 interacts with ZmHSF12-2 and represses the transcriptional regulation of ZmHSF12-2 on <i>ZmCKO2</i> and <i>ZmRAFS</i>. Co-overexpression of <i>ZmHSF12-1</i> and <i>ZmHSF12-2</i> in <i>Arabidopsis</i> not only improved the heat tolerance of plants but also compensated for the growth defect phenotype of <i>ZmHSF12-2</i> overexpressing <i>Arabidopsis</i> plants. These findings deepen our understanding of plant heat tolerance and significantly impact the scientific community. They support the potential application of co-overexpressing <i>ZmHSF12-1</i> and <i>ZmHSF12-2</i> to improve crop heat tolerance without causing growth retardation and yield compensation, thereby offering a promising avenue for crop improvement.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary legacy of the “living fossil” genus Parrotia (Hamamelidaceae): genomic insights into species divergence and polygenic adaptation","authors":"Yunyan Zhang,&nbsp;Kui Li,&nbsp;Yu Feng,&nbsp;Zhiyuan Li,&nbsp;Yimin Hu,&nbsp;Hamed Yousefzadeh,&nbsp;Malek Nasiri,&nbsp;Benjamin Adroit,&nbsp;Pengfu Li,&nbsp;Shan Lu,&nbsp;Pan Li,&nbsp;Hong Liu,&nbsp;Ye Peng,&nbsp;Chi Xu,&nbsp;Yingxiong Qiu,&nbsp;Zhongsheng Wang","doi":"10.1111/tpj.70367","DOIUrl":"https://doi.org/10.1111/tpj.70367","url":null,"abstract":"<p>Despite their long evolutionary history, the genomic basis of adaptation and speciation in “living fossil” plants remain largely unexplored. <i>Parrotia</i>, a Tertiary relict tree genus with two extant species, <i>P. subaequalis</i> and <i>P. persica</i>, exhibits a disjunct distribution between East Asia and West Asia. Here, we present the first chromosome-level assemblies for both species, confirmed their sibling relationship, and dated the speciation event to the early Miocene. The recent proliferation of long-terminal repeat retrotransposons has driven the genome expansion in <i>P. subaequalis</i>. We detected widespread heterogeneous genomic differentiation between species. Extensive signals of divergent selection, local adaptation, and elevated <i>K</i>a/<i>K</i>s ratios in <i>Parrotia</i> indicate that this genus has undergone adaptive evolution in distinct refugia, challenging the notion of it as an “evolutionary dead end”. Our findings provide new insights into the genomic evolution, environmental adaptation, and speciation of this “living fossil” tree genus.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70367","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High temperature induces MdGATA15 to suppress anthocyanin accumulation in apple peels","authors":"Yue Fang,&nbsp;Hua-Ying Ma,&nbsp;Zhi-Meng Wu,&nbsp;Chun-Fan Liu,&nbsp;Tong-Yao Xu,&nbsp;Tong Wang,&nbsp;Li-Xian Li,&nbsp;Shou-Qian Feng","doi":"10.1111/tpj.70381","DOIUrl":"https://doi.org/10.1111/tpj.70381","url":null,"abstract":"<div>\u0000 \u0000 <p>Although GATA transcription factors are known to play broad roles in plant growth, development, and stress responses, their involvement in high-temperature-induced anthocyanin suppression remains largely unexplored. In this study, using “Otome” as the experimental material, we revealed the important role of MdGATA15 in inhibiting anthocyanin accumulation under high temperature through multiple molecular mechanisms. A series of physiological and biochemical experiments demonstrated that MdGATA15 directly binds to the promoters of anthocyanin activators <i>MdMYB11</i>, <i>MdANS</i>, and the transporter gene <i>MdGSTF12</i>, repressing their expression. Simultaneously, MdGATA15 activates the expression of the anthocyanin biosynthesis repressor <i>MdMYB308</i>, further enhancing the inhibition. Notably, MdGATA15 binds to its own promoter, forming a positive feedback loop that significantly enhances its expression under high-temperature conditions. This mechanism provides new insights into understanding how apple responds to high-temperature stress. Additionally, we identified the bHLH transcription factor MdPIF4-Like3 in apple as an interactor of MdGATA15, which stabilizes and enhances the transcriptional activity of MdGATA15, thereby further reinforcing the inhibition of anthocyanin biosynthesis. These findings highlight the central role of MdGATA15 in high-temperature-mediated suppression of anthocyanin synthesis in apple and provide significant advances in understanding the molecular mechanisms of apple's response to heat stress. This study provides a theoretical basis for breeding heat-resistant apple cultivars with improved fruit quality by targeting key transcription factors involved in high-temperature stress response.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In conversation with Dr. Sateesh Kagale","authors":"Luis De Luna Valdez","doi":"10.1111/tpj.70355","DOIUrl":"https://doi.org/10.1111/tpj.70355","url":null,"abstract":"&lt;p&gt;In this interview, we speak with Dr. Sateesh Kagale, a leading plant biologist and Team Leader in Advanced Data Analytics at the National Research Council of Canada. Raised in a farming family in India, Dr. Kagale experienced firsthand the daily challenges that farmers face—from drought and poor soil fertility to pest outbreaks and plant diseases. These early life experiences instilled in him a deep-rooted commitment to improving agricultural sustainability and food security, shaping a career that now spans plant pathology, molecular biology, and bioinformatics. Dr. Kagale's research focuses on deciphering how crops respond to complex environmental stresses, with a particular emphasis on wheat, one of the world's most important staple crops. His team uses cutting-edge multi-omics approaches to unravel how wheat plants respond when faced with combinations of abiotic stresses like drought, heat, and salinity. Part of this work, titled ‘Multi-omics atlas of combinatorial abiotic stress responses in wheat’ was awarded TPJ's Outstanding Resource Article.&lt;/p&gt;&lt;p&gt;@SateeshKagale&lt;/p&gt;&lt;p&gt;\u0000 \u0000 &lt;/p&gt;&lt;p&gt;1. Can you tell us about you, your childhood, and your educational background? Anything that you're comfortable sharing.&lt;/p&gt;&lt;p&gt;I was born and raised in India in a farming family, where I experienced firsthand the many challenges that farmers face, from water shortages and pests to plant diseases and soil nutrient issues. These early experiences gave me a deep appreciation for agriculture and fueled my desire to find solutions that could help farmers, including my own family, overcome these obstacles.&lt;/p&gt;&lt;p&gt;With a clear sense of purpose, I pursued a B.Sc. in Agriculture at the University of Agriculture Sciences, Dharwad, India, followed by a Master's degree in Plant Pathology at the Tamil Nadu Agricultural University, Coimbatore, India. My passion for agricultural research then led me to Canada, where I completed a Ph.D. in Cell and Molecular Biology at Western University. This academic journey gave me the opportunity to explore innovative ways to improve crop resilience and productivity at a molecular level. In 2011, I joined the National Research Council of Canada as a research associate at the Aquatic and Crop Resource Development Research Centre in Saskatoon, Saskatchewan. In 2015, I transitioned to the role of research officer, and by April 2018, I assumed the position of team leader in Bioinformatics, now known as Advanced Data Analytics. Throughout my career, I have remained dedicated to advancing agricultural science and developing solutions to address real-world farming challenges. My work continues to be driven by the same motivation that first inspired me, that is, helping farmers build a more sustainable and productive future.&lt;/p&gt;&lt;p&gt;\u0000 \u0000 &lt;/p&gt;&lt;p&gt;2. How did you become interested in plant biology? Were you into plants growing up or that came later in life?&lt;/p&gt;&lt;p&gt;My childhood experiences with farming certainly s","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144712088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GhDMT7-mediated DNA methylation dynamics enhance starch and sucrose metabolism pathways to confer salt tolerance in cotton","authors":"Zhining Yang,&nbsp;Hongyu Nan,&nbsp;Xuke Lu,&nbsp;Maohua Dai,&nbsp;Kang Zhao,&nbsp;Yapeng Fan,&nbsp;Xiaopin Zhu,&nbsp;Menghao Zhang,&nbsp;Lidong Wang,&nbsp;Yuping Sun,&nbsp;Xiao Chen,&nbsp;Shuai Wang,&nbsp;Xiugui Chen,&nbsp;Junjuan Wang,&nbsp;Lanjie Zhao,&nbsp;Xiaowei Wang,&nbsp;Lixue Guo,&nbsp;Keyun Feng,&nbsp;Wenwei Gao,&nbsp;Wuwei Ye","doi":"10.1111/tpj.70364","DOIUrl":"https://doi.org/10.1111/tpj.70364","url":null,"abstract":"<div>\u0000 \u0000 <p>This study provides a comprehensive analysis of the impact of DNA methylation in cotton under salt stress conditions, elucidating its effects on gene expression and biological processes. Here, we determined the structures of the DNA methylation landscape across the cotton genome subjected to salt stress using whole-genome bisulfite sequencing (WGBS) and RNA-seq methodologies. We identified 4938 differentially methylated regions (DMRs) correlated with alterations in gene expression. Salt stress induced significant shifts in DNA methylation patterns, particularly in CHH contexts, suggesting context-dependent epigenetic regulation. DMRs were found to be implicated in diverse biological processes and pathways, encompassing protein metabolism, cellular homeostasis, starch and sucrose metabolism, and plant hormone signaling, all pivotal for cotton's adaptation to salt stress. Furthermore, RNA-seq analysis confirmed the impact of DNA methylation on gene expression, uncovering 9642 salt stress-responsive differentially expressed genes (DEGs). These DEGs exhibited enrichment in pathways such as carbohydrate metabolism, cell wall synthesis, and defense response, underscoring the intricate interplay between methylation and gene regulation in stress response. Moreover, the study investigated the role of the key DNA methyltransferase gene <i>GhDMT7</i> in modulating cotton's response to salt stress, revealing that its downregulation enhanced cotton's salt tolerance, potentially attributed to decreased DNA methylation levels, reduced membrane damage, and enhanced antioxidant capacity. These findings elucidate the role of DNA methylation in abiotic stress resilience and provide insights for crop improvement.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The miR156u/v-MhDIV3 Module Modulates Cadmium Uptake and Damage via Enhancing MhNRAMP1 Expression in Malus hupehensis.","authors":"Jianfei Song, Junhong Yan, Baozhen Sun, Jiaxin Lv, Bing Chen, Xiaojian Zhang, Xiaoyue Zhu, Weiwei Zhang, Hongqiang Yang","doi":"10.1111/pce.70088","DOIUrl":"https://doi.org/10.1111/pce.70088","url":null,"abstract":"<p><p>Cadmium (Cd), a toxic heavy metal, threatens crop production and human health, and its uptake by Malus hupehensis is regulated by MhNRAMP1. The role of the DIVARICATA (DIV) transcription factor (TF) in Cd stress remains largely unclear. Here, nine DIVs were isolated from M. hupehensis based on CDS sequences of identified DIV members in Malus. Among them, MhDIV3 exhibited the earliest and strongest response to Cd. Its encoded protein, MhDIV3, possesses the characteristics of R2R3-MYB TF. Suppression of MhDIV3 in M. hupehensis roots and apple calli led to a higher fresh weight and lower levels of reactive oxygen species (ROS) and malondialdehyde (MDA) under Cd stress, while overexpression of MhDIV3 in M. hupehensis roots and tomato exacerbated Cd-caused oxidative damage by accelerating Cd²⁺ uptake. Mechanistically, MhDIV3 bound to an enhancer in the intron of MhNRAMP1 to positively regulate its expression under Cd stress. Additionally, miR156u/v, an upstream regulator of MhDIV3, suppressed MhDIV3 expression by complementing its 3'UTR. Overexpression of miR156u/v reduced Cd²⁺ uptake and stress damage, similar to MhDIV3-suppression. Overall, our results suggested that miR156u/v-MhDIV3 module positively modulates Cd uptake and damage by triggering MhNRAMP1 expression in M. hupehensis.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phase 2 randomized controlled trial of seasonal influenza vaccine shows Advax^® delta inulin adjuvant accelerates the humoral anti-influenza response.","authors":"Lei Li, Yoshikazu Honda-Okubo, Varun Khanna, Dimitar Sajkov, Nikolai Petrovsky","doi":"10.1111/imcb.70050","DOIUrl":"https://doi.org/10.1111/imcb.70050","url":null,"abstract":"<p><p>Advax^® is a delta inulin polysaccharide adjuvant shown in animal models to enhance and accelerate influenza vaccine protection. A clinical trial was conducted in 109 healthy adult participants aged 18-70 years randomized to receive a single intramuscular seasonal trivalent influenza vaccine (TIV) alone or formulated with 5 or 10 mg Advax^® adjuvant to explore the effect of the adjuvant on the humoral immune response. The addition of Advax^® 10 mg to TIV accelerated the rise in serum influenza-specific antibodies, with this group exhibiting significantly higher increases in hemagglutinin inhibition (HAI) against 3 of the 3 vaccine serotypes at 7 days post-vaccination (7 dpv), 2 at 14 dpv and 1 at 21 dpv. By 7 dpv, the Advax 10-mg group achieved HAI seroprotection rates of 96.9% against H1N1, 100% against H3N2 and 46.9% against influenza B versus rates of 86.1%, 100% and 22.2%, respectively, for the TIV alone group. The Advax^®-adjuvanted groups demonstrated an increased frequency of non-silent CDR3 mutations in the B cell receptor heavy chain of peripheral blood IgG⁺ and IgM⁺ plasmablasts at 7 dpv, consistent with the adjuvant enhancing B cell affinity maturation in IgM⁺ and IgG⁺ plasmablasts independently of class switch recombination. The ability of Advax adjuvant to accelerate humoral responses against influenza could be advantageous during influenza outbreaks when time to protection is of the essence. Further studies are needed into the mechanisms whereby delta inulin accelerates vaccine immunity.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A phloem-based defense mechanism linked to elevated riboflavin levels in wild tomato Solanum chmielewskii impedes whitefly nymphal development","authors":"Lissy-Anne M. Denkers,&nbsp;Arjen van Doorn,&nbsp;Marc Galland,&nbsp;Gerd Balcke,&nbsp;Martin de Vos,&nbsp;Robert C. Schuurink,&nbsp;Petra M. Bleeker","doi":"10.1111/tpj.70363","DOIUrl":"https://doi.org/10.1111/tpj.70363","url":null,"abstract":"<p>Management of the phloem-feeding pest insect <i>Bemisia tabaci</i> (whitefly) is difficult due to its short generation time and large number of offspring. Several whitefly-resistant wild tomato accessions have been identified, with the resistance attributed to specific defense metabolites in glandular trichomes. Interestingly, we found that on <i>Solanum chmielewskii</i> LA1840, which lacks trichome-based resistance, nymphal development is delayed and decreased compared with a cultivated tomato. Here, we show that the resistance observed in LA1840 is based on a mobile factor in the vasculature, the site of interaction for nymphs during feeding. The putative compound responsible for the resistance apparently passed the graft junction from an LA1840 rootstock to an otherwise susceptible cultivar scion. After untargeted metabolomics on the phloem collected from the wild accessions, a Random Forest algorithm predicted riboflavin to be linked to the resistance phenotype. The resistant genotypes indeed exhibit increased riboflavin levels in leaves compared with susceptible plants. The effect of elevated riboflavin levels on whitefly nymph development was validated through feeding riboflavin to susceptible plants. Our results highlight the power of natural variation in metabolites and vasculature-based resistance mechanisms for the development of sustainable whitefly management.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70363","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AtHSP90.5 and AtFtsH12 synergistically regulate the accumulation of photosynthesis protein complexes and chloroplast development in Arabidopsis","authors":"Xiaomin Wang,&nbsp;王晓敏,&nbsp;Jiahui Zhong,&nbsp;钟家辉,&nbsp;Bilang Li,&nbsp;李碧浪,&nbsp;Shuo Yang,&nbsp;杨硕,&nbsp;Peiyi Wang,&nbsp;王佩仪,&nbsp;Jingxia Shao,&nbsp;邵景侠,&nbsp;Jingjing Meng,&nbsp;孟晶晶,&nbsp;Xiayan Liu,&nbsp;刘夏燕,&nbsp;Fei Yu,&nbsp;郁飞,&nbsp;Yafei Qi,&nbsp;齐亚飞","doi":"10.1111/tpj.70369","DOIUrl":"https://doi.org/10.1111/tpj.70369","url":null,"abstract":"<div>\u0000 \u0000 <p>The 90-kDa heat shock protein (HSP90) is a central component of the chaperone system for protein homeostasis (proteostasis). In Arabidopsis, AtHSP90.5 is the sole chloroplast-localized HSP90 family member, yet its role in chloroplast proteostasis remains poorly characterized. Here, we identify and characterize the <i>pale green arabidopsis 5</i> mutant, <i>pga5-1</i>, which exhibits defective chloroplast development and impaired accumulation of photosynthetic protein complexes. Genetic analysis revealed that <i>pga5-1</i> is a hypomorphic allele of <i>AtHSP90.5</i>, harboring a missense mutation (G646E) localized closely to the substrate-binding site. Biochemical studies demonstrated that AtHSP90.5 interacts with AtFtsH12, and the ATPase activity of AtHSP90.5 is essential for the oligomerization of AtFtsH12 complexes. Strikingly, the mutation of the conserved residue (E106A) for the ATPase activity of AtHSP90.5 can rescue the embryonic lethality of <i>AtHSP90.5</i> null mutants, yielding albino seedlings with non-photosynthetic plastids, and partially complement <i>pga5-1</i>. Furthermore, we show that AtHSP90.5 associates with subunits of light-harvesting antenna complexes, including LhcB1, LhcB2, and LhcA1, and is epistatic to PGA4/cpSRP54 in regulating the accumulation of a chimeric chloroplast marker protein, LhcB2-GFP. Collectively, our findings establish a critical role for AtHSP90.5 in maintaining photosynthesis protein complexes and uncover a previously unknown functional link between AtHSP90.5 and AtFtsH12 in chloroplast protein translocation.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}