生命科学最新文献

{"title":"The E3 ubiquitin ligase SCFEDL3 mediates salt stress response by degradation of IbPP2CA2 to regulate ABA signaling in sweetpotato","authors":"Dandan Wang,&nbsp;Chengyang Li,&nbsp;Weihan Song,&nbsp;Wei Tang,&nbsp;Yuyu Chen,&nbsp;Meng Kou,&nbsp;Runfei Gao,&nbsp;Tianqi Gao,&nbsp;Chen Li,&nbsp;Hui Yan,&nbsp;Aicen Zhang,&nbsp;Xin Wang,&nbsp;Yungang Zhang,&nbsp;Qiang Li","doi":"10.1111/tpj.70307","DOIUrl":"https://doi.org/10.1111/tpj.70307","url":null,"abstract":"<div>\u0000 \u0000 <p>The phytohormone abscisic acid (ABA) plays a critical role as a signaling molecule, mediating adaptive responses of plants to salt stress. However, the orchestration of ABA signaling in response to salt stress in sweetpotato remains poorly understood. In this study, we cloned a gene of unknown function, <i>IbEDL3</i>, which is significantly upregulated in sweetpotato under salt stress conditions. Transgenic sweetpotato plants overexpressing <i>IbEDL3</i> exhibited enhanced proline accumulation, reaction oxygen species (ROS) scavenging, stomatal closure, and Na⁺ efflux compared with wild-type (WT) plants under salt stress. Further investigation revealed that IbEDL3 interacts with IbSKP1-1, forming part of the E3 ubiquitin ligase SCF^EDL3 complex. Although IbEDL3 interacted with IbABI1, IbABI2, and IbPP2CA2, the SCF^EDL3 complex only ubiquitinated and degraded IbPP2CA2. Under salt stress, SCF^EDL3 accelerated the ubiquitination and degradation of IbPP2CA2, leading to the release of more IbSnRK2.6, which further promoted ABA signaling to regulate stomatal closure, proline accumulation and Na⁺ efflux. Inhibition of <i>IbPP2CA2</i> resulted in enhanced salt tolerance in sweetpotato. Collectively, the SCF^IbEDL3-IbPP2CA2 module provides insight into the mechanisms underlying ABA signaling in response to salt stress and offers a new avenue for genetic improvement of salt tolerance in sweetpotato.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Safety Margins and Peak Leaf Temperatures Predict Vulnerability of Diverse Plant Species to an Experimental Heatwave.","authors":"Diana Cox, Renée M Marchin, David S Ellsworth, Agnieszka Wujeska-Klause, Alessandro Ossola, Kristine Y Crous, Michelle R Leishman, Paul D Rymer, Mark G Tjoelker","doi":"10.1111/pce.70041","DOIUrl":"https://doi.org/10.1111/pce.70041","url":null,"abstract":"<p><p>Extreme heat can push plants beyond their thermal safety margin (TSM) if maximum leaf temperature (T_{leaf_max}) exceeds leaf critical temperature (T_crit). The TSM is potentially useful for assessing heat vulnerability across species but needs further validation, so we exposed 50 tree/shrub species in controlled glasshouses to a 6-day heatwave (peak air temperature = 41°C). Many species increased their mean T_crit during the heatwave (42%), with ΔT_crit ranging from +1°C to 4°C, but other species did not acclimate or were impaired by heat stress (58%). Species T_{leaf_max} explained ~55% of the variation in species T_crit and was a key correlate of the plasticity of T_crit among species. Species with high ΔT_crit also had higher ΔT_{leaf_max}, with leaves being 7°‒12°C hotter during the heatwave than under baseline conditions. Both T_{leaf_max} and TSMs were correlated with heatwave damage across diverse species from contrasting climate zones. Species differences in TSMs were stable across measurement temperatures, correctly identified the most vulnerable species, and were strongly associated with T_{leaf_max}. Our results suggest that (1) T_{leaf_max} alone is more informative than T_crit for ranking species heat tolerance, and (2) species vulnerability to heatwaves is most reliably assessed by using TSMs that integrate T_{leaf_max} with T_crit across species.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of the Receptor-Like Kinase BIR1 Causes SOBIR1- and EDS1-Dependent Cell Death Phenotypes in Arabidopsis.","authors":"Irene Guzmán-Benito, Carmen Robinson, Marta Núñez-Salvador, Isabel Punzón, Gustavo Gómez, César Llave","doi":"10.1111/pce.70036","DOIUrl":"https://doi.org/10.1111/pce.70036","url":null,"abstract":"<p><p>The receptor-like kinase BAK1-INTERACTING RECEPTOR-LIKE KINASE 1 (BIR1) negatively regulates multiple resistance signalling pathways in Arabidopsis thaliana. Previous studies showed that loss of BIR1 function causes extensive cell death and constitutive activation of immune responses. Using a dexamethasone (DEX)-inducible system, we investigated the effects of BIR1 overexpression on plant development and immunity. Overexpression of BIR1, in the absence of microbes or elicitors, led to cell death phenotypes that resembled the effects of BIR1 depletion in knockout plants. We also observed transcriptional outputs that greatly overlap with canonical pathogen-triggered immunity and effector-triggered immunity (ETI), suggesting that BIR1 modulates immune responses by influencing both pathways. To investigate the genetic basis of BIR1 phenotypes, we conditionally expressed BIR1 in various Arabidopsis immune mutants including sobir1, bak1, eds1, sid2 and eds5. We found that ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) and SUPPRESSOR OF BIR1-1 (SOBIR1) are necessary for ETI-type cell death seen with BIR1 overexpression. These results support the hypothesis that an excess of BIR1 may be detected by guarding NLR proteins, triggering a cell death response in which SOBIR1 and EDS1 cooperate to transduce signals downstream of R proteins.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sketching T cell atlases in the single-cell era: challenges and recommendations.","authors":"Itana Bojović, António Gg Sousa, Sini Junttila, Laura L Elo","doi":"10.1111/imcb.70040","DOIUrl":"https://doi.org/10.1111/imcb.70040","url":null,"abstract":"<p><p>Recent advances in single-cell technologies have enabled the creation of comprehensive cell atlases, reference maps of various cell types within organisms. Here we specifically focus on T cell atlases, which offer a detailed catalog of the adaptive immune system at single-cell resolution. As such, they capture cellular diversity, functional states, and spatial dynamics across tissues, developmental stages, and disease conditions. Given the central role of T cells in orchestrating immune responses, their dysregulation underpins autoimmune disorders, cancer progression and failed immunotherapies. Therefore, a unified T cell atlas is critical for decoding such disease mechanisms, identifying therapeutic targets, and advancing personalized treatments. In this article, we explore the latest advances in T cell atlases, describing breakthroughs in multi-omics technologies, spatial profiling and computational frameworks that resolve transcriptional, epigenetic and proteomic heterogeneity. We also address persistent challenges and highlight strategies to address these gaps. Finally, we discuss emerging frontiers set to reshape our understanding of T cell dynamics in both health and diseases. Together, these insights underscore the transformative potential of T cell atlases in reconstructing precision immunology and accelerating therapeutic innovation.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-Dependent Modulation of ECS-Mediated Haploid Induction in Arabidopsis thaliana.","authors":"Zifu Zhao, Hedi Ma, Xuecheng Zhang, Ce Shi, Meng-Xiang Sun","doi":"10.1111/pce.70054","DOIUrl":"https://doi.org/10.1111/pce.70054","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foliar Water Uptake Capacity in Sun and Shade Leaves of Fagus sylvatica L.","authors":"Willem Goossens, Olivier Leroux, Kathy Steppe","doi":"10.1111/pce.70035","DOIUrl":"https://doi.org/10.1111/pce.70035","url":null,"abstract":"<p><p>Foliar water uptake (FWU) is emerging as a key strategy for coping with drought, prompting more and deeper investigation into its role. This study examines the FWU dynamics by a series of time-dependent submersion experiments in sun and shade beech (Fagus sylvatica L.) leaves, a species known for its drought sensitivity. The results showed distinct FWU capacities, linked to their varying leaf water potentials, indicating that this factor is the driver for foliar absorption. Both sun and shade leaves experienced a proportional increase in leaf water content subsequent to FWU, with the same increment across leaf types after normalising for leaf water potential. Furthermore, results uncovered distinct foliar absorption dynamics between sun and shade leaves, pointing to specific absorption strategies. While sun leaves were able to absorb more water, they approached saturation more gradually compared to their shaded counterparts. This divergence in water management strategy also reflects the morpho-anatomical adaptation of sun and shade leaves to their respective microclimates.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delta inulin alone or combined with CpG oligonucleotide enhances antibody-dependent influenza vaccine protection in mice and nonhuman primate newborns.","authors":"Isaac G Sakala, Yoshikazu Honda-Okubo, Nikolai Petrovsky","doi":"10.1111/imcb.70045","DOIUrl":"10.1111/imcb.70045","url":null,"abstract":"<p><p>Newborns represent over half of hospitalized pediatric influenza infection cases, with current influenza vaccines not effective in the first months of life. Advax^® (delta inulin) is a polysaccharide particle that targets DC-SIGN, whereas CpG55.2 is a potent murine and human toll-like receptor (TLR)-9 agonist. This study asked whether Advax or CpG alone, or combined, could enhance the protection of an inactivated influenza virus vaccine (IIV) in newborns. One-day-old mouse pups were immunized subcutaneously with a single dose of IIV alone or with Advax or Advax-CpG55.2 adjuvants and then, at 28 days of age, challenged intranasally with a lethal dose of influenza virus. While IIV alone or with CpG adjuvant provided minimal protection, Advax alone or combined with CpG55.2 induced enhanced serum anti-influenza IgM and IgG responses to IIV and protected the newborns against clinical disease. Protection induced by a single vaccine dose was highly durable and was still evident 6-9 months after a single neonatal immunization. Protection was lost in B-cell-deficient μMT pups but preserved in β2m knockout pups and in CD4⁺ and CD8⁺ T-cell-depleted pups, indicating the importance of intact humoral immunity to the enhanced protection. The neonatal benefits of Advax^® and Advax-CpG55.2 adjuvant were confirmed in newborn macaques, where they similarly enhanced serum anti-influenza antibody responses to IIV. This raises the possibility that Advax^® adjuvant alone or in combination with CpG55.2 may have utility in improving influenza vaccine protection in human newborns.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solving the Defense-Yield Dilemma in Crop Plants.","authors":"Subhasis Karmakar, S P Avinash, Sabarinathan Selvaraj, Mirza J Baig","doi":"10.1111/pce.70043","DOIUrl":"https://doi.org/10.1111/pce.70043","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phototropin Mediates the Accurate Movement of Trifoliate Leaves in Medicago truncatula.","authors":"Zhicheng Jiao, Silu Zhan, Chaowei Liu, Hanyin Zhang, Wanying Huang, Faqiang Li, Liangfa Ge","doi":"10.1111/pce.70025","DOIUrl":"https://doi.org/10.1111/pce.70025","url":null,"abstract":"<p><p>Light-harvesting efficiency is crucial for plant photosynthesis, and leaves must adjust their angles to maximize sunlight capture. Leguminous plants have evolved a specialized motor organ, the pulvinus, located between the leaf blades and petioles, enabling rapid leaflet reorientation toward sunlight. Although the role of pulvinus in orienting leaflets is well understood, the exact mechanisms behind this light response remain unclear. In this study, we identified a fast-neutron radiated mutant with the back-bent leaflet (bbl) phenotype in Medicago truncatula. The mutant leaves fail to orient properly toward light and excessively open under white light (WL) or blue light (BL). The adaxial motor cells of the bbl pulvinus contain a higher potassium concentration than those of wild-type plants, causing excessive cell swelling under WL. BBL encodes a Phototropin 2 homologue from Arabidopsis thaliana, and is highly expressed in the pulvinus. BBL/MtPHOT2 is associated with the plasma membrane in the dark, and translocates to the Golgi body upon BL exposure. Our findings demonstrate that BBL/MtPHOT2 regulates leaflet movement by modulating potassium concentration in the adaxial pulvinus motor cells, ensuring accurate leaf orientation toward the light. Our study provides genetic evidence that fills the gap between pulvinus-mediated leaflet movement and light direction.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis Vacuolar protein sorting 9a (VPS9a) is required for glutamine synthetase/glutamate synthase (GS/GOGAT) cycle and autophagy under nutrient starvation","authors":"Baiyang Yu,&nbsp;Yanhui Zhou,&nbsp;Yunfeng Shi,&nbsp;Shengshu Wang,&nbsp;Qing Pang,&nbsp;Chao Yang,&nbsp;Thomas Roitsch,&nbsp;Weiming Hu,&nbsp;Yizhou Wang,&nbsp;Fen Liu","doi":"10.1111/tpj.70289","DOIUrl":"https://doi.org/10.1111/tpj.70289","url":null,"abstract":"<div>\u0000 \u0000 <p>Plants have developed complex endomembrane systems in response to environmental challenges such as nutrient deficiency. This study focused on the role of <i>Vacuolar protein sorting 9 (VPS9a)</i>, a key regulatory gene involved in the endosomal sorting process in <i>Arabidopsis thaliana</i>. Loss of <i>VPS9a</i> function results in stress-sensitive phenotypes under carbon and nitrogen starvation. First, we investigated the changes in the Glutamine Synthetase/Glutamate Synthase (GS/GOGAT) cycle under nitrogen starvation and conducted a co-expression network analysis based on transcriptomic profiles. These results indicate that the endocytic pathway and the majority of the degradation processes are related to GS and NADH-GOGAT activity. Genes related to autophagy and endocytic pathways showed diverse response trends in Col-0, <i>vps9a-2</i>, and <i>35S:VPS9a-GFP/vps9a-2</i>. Several autophagy- and endocytosis-related genes, including <i>Autophagy-related protein 1</i> (<i>ATG1</i>), <i>Autophagy-related protein 8</i> (<i>ATG8)</i>, <i>Thylakoid lumen protein</i> (<i>TLP18.3</i>), <i>Autoinhibited Ca(2+)-ATPase, Isoform 4</i> (<i>ACA4</i>), <i>MAP kinase 2</i> (<i>AtMKK2</i>), and <i>Extensin 21</i> (<i>EXT21</i>), were identified as hub genes. Further, we found that the loss of <i>VPS9a</i> function leads to reduced accumulation of autophagic bodies and a marked decrease in ATG8a protein levels but does not affect autophagic flux or the accumulation of ATG8 with phosphatidylethanolamine (PE). Interestingly, <i>VPS9a</i> appears to exert differential effects on various ATG8 Homologs. In summary, our results established a connection between autophagy, endocytic pathways, and nitrogen metabolism processes, identifying key hub genes involved in these processes. Among these hub genes, <i>VPS9a</i> was found to affect ATG8a levels, suggesting that <i>VPS9a</i> selectively regulates specific ATG8 proteins involved in autophagic processes.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}