{"title":"PsoRPM3 Recognises the Meloidogyne incognita Effector MiTSPc to Trigger Defence Response in Prunus sogdiana.","authors":"Wenjiang Pu, Xuefeng Chen, Zhikun Liu, Haifeng Zhu, Sifang Luo, Kun Xiao, Jianfang Hu, Pingyin Guan","doi":"10.1111/pce.70086","DOIUrl":"https://doi.org/10.1111/pce.70086","url":null,"abstract":"<p><p>Phytoparasitic nematodes are among the most economically destructive plant pathogens. Large numbers of effectors secreted by phytoparasitic nematodes are delivered into host cells to facilitate susceptible invasion and maintain long-lasting parasitism in the host plants. Plant nucleotide-bound leucine-rich repeat (LRR) receptors (NLRs) directly or indirectly recognise pathogen-derived effectors to initiate innate immunity. In this study, we have identified Meloidogyne incognita secreted effectors MiTSPc and MiACPS, which can interact with resistance protein PsoRPM3 in Prunus sogdiana (P. sogdiana). In the leaves of PsoRPM3 transgenic tobacco plants and disease-resistant P. sogdiana lines, when the MiTSPc and MiACPS were transit expressed, significant hypersensitive response and high ion leakage rate were detected. Moreover, when the MiTSPc was silenced in M. incognita, galls were observed in the roots of PsoRPM3 transgenic tobacco plants. Co-localisation experiments have shown that MiTSPc and PsoRPM3 were overlapped. Our data revealed that LxxLxLxxN/CxL motif of PsoRPM3 LRR domain can recognise MiTSPc<sup>23-54aa</sup>. Taken together, the disease resistant protein PsoRPM3 can directly recognise M. incognita effector MiTSPc to deploy defence responses in P. sogdiana.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Breaking the silence: tethering a translational enhancer to improve transgene expression","authors":"Martin Balcerowicz","doi":"10.1111/tpj.70375","DOIUrl":"https://doi.org/10.1111/tpj.70375","url":null,"abstract":"<p>Genetic transformation has become a routine technique in plant biology: transgenes are widely used as tools in fundamental research, as expression systems for high-value proteins and—despite advances in CRISPR-based gene editing—remain the method of choice to introduce traits absent from a species' breeding pool. However, these applications are frequently hampered by gene silencing, which can lead to the decline or even complete loss of transgene expression over successive generations. This effect has been attributed to two major processes: transcriptional gene silencing (TGS) via methylation of the transgene DNA, and post-transcriptional gene silencing (PTGS) mediated by the RNA interference (RNAi) pathway (Molnar et al., <span>2011</span>). PTGS involves the formation of short-interfering RNAs (siRNAs) of 21–25 nucleotides that are complementary to the transgene's mRNA. These siRNAs are loaded into RNA-induced silencing complexes, guiding them to their target mRNA for degradation or translational inhibition. PTGS typically precedes TGS, and continuous production of siRNAs can trigger activation of RNA-directed DNA methylation of a transgene's promoter regions (Matzke & Mosher, <span>2014</span>).</p><p>Keith Slotkin and his lab investigate gene silencing mechanisms in plants, and as part of this broader effort, also explore strategies to improve transgene expression. They recently leveraged the RNA-binding protein BRUNO-LIKE 1 (BRN1) to establish an <i>in vivo</i> protein–mRNA tethering system (Cuerda-Gil et al., <span>2022</span>). BRN1 binds a seven-nucleotide recognition sequence in the 3′ UTR of the <i>SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1</i> (<i>SOC1</i>) transcript and thereby interferes with its translation (Kim et al., <span>2013</span>). A truncated BRN1 RNA-binding domain (BD), while still able to bind the <i>SOC1</i> 3′ UTR, does not repress translation and can be fused to other proteins to influence the fate of the tethered mRNA. For example, fusion of a deadenylase protein to BD triggered <i>SOC1</i> transcript deadenylation and subsequent degradation, while fusion of the conserved 40S ribosomal subunit RIBOSOMAL PROTEIN S6 (RPS6) increased translation efficiency (Cuerda-Gil et al., <span>2022</span>).</p><p>Senior Research Scientist Yu-Hung Hung, first author of the highlighted paper, extended this approach to test whether the BD-RPS6 tethering system can be used to improve expression of transgenes. As a target Hung and colleagues chose Cas9, a widely used transgene that generates a quantifiable output. They generated Cas9 expression constructs with different BRN1 binding site configurations at the 3′ end: no (0xBS), one (1xBS) or four (4xBS) BRN1 binding sites, or the full <i>SOC1</i> 3′ UTR (Figure 1A). These constructs were expressed together with a guide RNA targeting the <i>ALCOHOL DEHYDROGENASE 1</i> (<i>ADH1</i>) gene and transformed into Arabidopsis plants with or without the BD-RPS6 tethering system.</p><p>To","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70375","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenwen Zhu, Lei Wang, Jinzheng Wang, Ni Zhan, Zhongtian Shi, Yuying Sun, Qiang Lv, Yong Hu, Fang Bao, Ling Li, Yikun He, Yu Wang
{"title":"Nitric oxide delays floral transition in Arabidopsis by inhibiting histone deacetylases HDA5 and HDA6","authors":"Wenwen Zhu, Lei Wang, Jinzheng Wang, Ni Zhan, Zhongtian Shi, Yuying Sun, Qiang Lv, Yong Hu, Fang Bao, Ling Li, Yikun He, Yu Wang","doi":"10.1111/tpj.70379","DOIUrl":"https://doi.org/10.1111/tpj.70379","url":null,"abstract":"<div>\u0000 \u0000 <p>Nitric oxide (NO), a reactive small molecule, plays a critical role in various developmental and physiological processes in living organisms. Previous studies by our group revealed that NO delays flowering in Arabidopsis by increasing transcript levels of the flowering repressor <i>FLOWERING LOCUS C</i> (<i>FLC</i>). In this study, we further investigated the molecular mechanism by which NO regulates <i>FLC</i> expression. Genetic experiments demonstrated that NO-induced delayed flowering specifically depends on elevated <i>FLC</i> transcript levels. Chromatin Immunoprecipitation assays revealed that NO significantly enhances histone H3 acetylation at the <i>FLC</i> locus. Biochemical analyses further showed that NO reduces total histone deacetylase activity through <i>S</i>-nitrosylation of histone deacetylases HDA5 and HDA6. Additionally, we identified and evaluated potential <i>S-</i>nitrosylation sites on HDA5 and HDA6, revealing their effects on deacetylase activity and floral regulation. Collectively, our findings uncover a novel mechanism by which NO mediates epigenetic modification to modulate flowering in Arabidopsis. This study sheds light on the functional network linking NO signaling, epigenetic modification, and flowering.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sean M. Robertson, Solihu Kayode Sakariyahu, Elisa Gan, Obaid Maqsood, Asher Pasha, Nicholas J. Provart, Olivia Wilkins
{"title":"Growth-limiting drought increases sensitivity of Asian rice (Oryza sativa) leaves to heat shock through physiological and spatially distinct transcriptomic responses","authors":"Sean M. Robertson, Solihu Kayode Sakariyahu, Elisa Gan, Obaid Maqsood, Asher Pasha, Nicholas J. Provart, Olivia Wilkins","doi":"10.1111/tpj.70349","DOIUrl":"https://doi.org/10.1111/tpj.70349","url":null,"abstract":"<p>Growth-limiting droughts (GLD) impair tissue expansion and delay developmental transitions but are often not considered as stressors, as many physiological traits are only slightly altered relative to well-watered counterparts. Concurrently, cell size, biochemical makeup, and transcriptome profiles vary along the leaf blade in accordance with the partitioning of distinct functions to spatially defined regions of the leaf. This suggests that because different parts of the leaf have underlying differences in their transcriptome profiles, they might respond to GLD in distinctive ways. Moreover, how antagonistic stressors influence physiology and gene expression in different zones of leaves is an open question. In this study, we profiled growth, anatomy, and gas exchange in Asian rice (<i>Oryza sativa</i>) leaves developed in well-watered and GLD conditions, with or without a secondary heat shock. We dissected leaves into seven equal-length segments for transcriptome analysis in these conditions. We hypothesized that GLD would make the leaves more sensitive to heat shock and would disrupt the underlying heterogeneity of the leaf transcriptome. GLD plants were more strongly affected by heat shock with respect to gas exchange and the number and types of genes that were differentially expressed and that these differences varied along the leaf blade. We developed an eFP browser tool with these data to facilitate exploration and hypothesis testing. These findings show that even mild drought treatments are sufficient to impact responses to antagonistic stressors and that substantial within-organ variance exists with respect to stress responses.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70349","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TaSG-D1–TaGAMyb signaling module regulates seed weight in wheat (Triticum aestivum L.)","authors":"Yan Zhou, Guangxian Cui, Shijun Wei, Xingyuan Xi, Jiaqi Zhang, Hongjiao Jiang, Jie Cao, Baoyue Zhang, Yumei Zhang, Huiru Peng, Yingyin Yao, Zhaorong Hu, Zhongfu Ni, Ive De Smet, Qixin Sun, Mingming Xin","doi":"10.1111/tpj.70377","DOIUrl":"https://doi.org/10.1111/tpj.70377","url":null,"abstract":"<p>Grain weight is one of the critical determinants of wheat (<i>Triticum aestivum</i> L.) yield, and understanding its genetic and molecular mechanisms is essential for improving crop productivity. Here, we find that <i>TaSG-D1</i>, which encodes an STKc-GSK3 kinase, negatively regulates grain weight. TaSG-D1 interacts with and phosphorylates TaGAMyb to reduce its degradation. Overexpression of <i>TaGAMyb</i> results in decreased grain length and weight, whereas its knockout increases both agronomic traits in wheat. Further investigation reveals that TaGAMyb directly activates the expression of <i>TaCKX2.2.1</i>, a negative regulator of grain development. Transcriptome profiling shows differential expression of <i>TaCKX2.2.1</i> in 15-DAP grain of WT and <i>TaGAMyb</i> knockout lines, ultimately leading to a decreased concentration of active cytokinin in grains. Taken together, our findings demonstrate that <i>TaSG-D1</i> negatively regulates grain development by increasing the protein abundance of transcription factor TaGAMyb, which in turn promotes the expression of downstream gene <i>TaCKX2.2.1</i>, a key regulator of cytokinin signaling.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maxence James, Jacques Trouverie, Benoit Bernay, Anne Marmagne, Fabien Chardon, Marin-Pierre Gemin, Philippe Etienne, Céline Masclaux-Daubresse
{"title":"V-ATPases and amino acid catabolism are strongly disturbed in the roots of autophagy mutants in Arabidopsis, impacting water use efficiency and tricarboxylic acid metabolism","authors":"Maxence James, Jacques Trouverie, Benoit Bernay, Anne Marmagne, Fabien Chardon, Marin-Pierre Gemin, Philippe Etienne, Céline Masclaux-Daubresse","doi":"10.1111/tpj.70359","DOIUrl":"https://doi.org/10.1111/tpj.70359","url":null,"abstract":"<p>Autophagy is essential for homeostasis and nutrient recycling. Its activity increases with aging and in response to deficiencies. The effects of defective autophagy on root metabolism have not yet been described. Addressing this question through root proteome analyses, we found that most V-ATPases were less abundant in the roots of autophagy mutants than in wild type. V-ATPases deficit, associated with lower root water contents and lower nitrate, magnesium, and potassium concentrations, indicated that the disturbance of cellular ion and water management in autophagy mutants was likely related to vacuole function. Isotopic δ<sup>13</sup>C analyses and leaf temperature measurements using thermography showed that water deficit in autophagy mutants was not due to excess transpiration, as the conductance of stomata was reduced in mutants compared to wild type. Many proteins related to the catabolism of amino acids and lipids and the tricarboxylic acid (TCA) cycle were over-abundant in <i>atg</i> mutants. The increase in several proteases that paralleled amino acid catabolism suggested that in the absence of autophagic flux, compensatory processes could be established to degrade proteins, recycle amino acids, and fuel TCA. Whether the V-ATPases defect affects energy metabolism and promotes lipid and amino acid catabolism to compensate and fuel TCA remains to be explored. In conclusion, this report establishes for the first time a correlation between autophagy and vacuole function through V-ATPases, particularly with regard to water and ion management. Additionally, this report shows the exacerbation of amino acid catabolism in relation to the stimulation of the TCA cycle in autophagy mutants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70359","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuai Shao, Bingbing Lv, Mei Wang, Sijia Zeng, Shiying Wang, Zisong Yang, Pengda Ma
{"title":"Biosynthesis and regulatory mechanism of tanshinones and phenolic acids in Salvia miltiorrhiza","authors":"Shuai Shao, Bingbing Lv, Mei Wang, Sijia Zeng, Shiying Wang, Zisong Yang, Pengda Ma","doi":"10.1111/tpj.70358","DOIUrl":"https://doi.org/10.1111/tpj.70358","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Salvia miltiorrhiza</i>, a perennial plant of the genus <i>Salvia</i> in the family Lamiaceae, is one of the most important traditional Chinese medicinal herbs, renowned for its significant economic and medicinal value. Its application in China dates back to 200 BC, where it has been utilized clinically either as a monotherapy or in combination with other herbal medicines for treating cardiovascular and cerebrovascular diseases, as well as various other ailments. The bioactive constituents of <i>S. miltiorrhiza</i> primarily include lipophilic tanshinones and hydrophilic phenolic acids. Over the past decades, the biosynthetic pathways of tanshinones and phenolic acids have been elucidated. Coupled with the sequencing of its genome, substantial progress has been made in deciphering the biosynthesis and regulatory mechanisms of bioactive compounds in <i>S. miltiorrhiza</i>, including tanshinones, phenolic acids, flavonoids, and prenylated quinones. This review summarizes recent advances in the regulatory mechanisms underlying the biosynthesis of phenolic acids and tanshinones in <i>S. miltiorrhiza</i>, focusing on transcriptional regulation, post-translational modifications, and epigenetic regulation. These insights provide a foundation for enhancing the production of bioactive compounds through biotechnological approaches and advancing pharmacological applications.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dynamic m6A RNA methylation correlates with ethylene-induced petal senescence and may modulate antioxidant and metabolic pathways in carnation (Dianthus caryophyllus L.)","authors":"Hongya Wang, Chuansen Zhao, Zekang Pan, Xilin Li, Siqi Wang, Xinyi Yuan, Chunlin Zhu, Zheng Sun, Min Wang, Shan Feng, Zeqing Li, Linlin Zhong, Yunjiang Cheng, Manzhu Bao, Fan Zhang","doi":"10.1111/tpj.70318","DOIUrl":"https://doi.org/10.1111/tpj.70318","url":null,"abstract":"<div>\u0000 \u0000 <p>Petal senescence is a critical stage in the life cycle of plants and involves a complex regulatory network, in which ethylene is one of the important regulatory factors. As a key RNA modification, the role of m<sup>6</sup>A methylation in various physiological processes in plants has been widely recognized, but its specific function in petal senescence has not been thoroughly investigated. In this study, LC–MS/MS analysis showed that ethylene treatment significantly reduced the level of m<sup>6</sup>A in carnation (<i>Dianthus caryophyllus</i> L.) petals. MeRIP-seq analysis further showed that ethylene treatment resulted in a change in the distribution pattern of m<sup>6</sup>A modification, especially an increase in enrichment near the stop codon region. Further studies identified four aging-related genes, including antioxidant genes (<i>DcGST10</i> and <i>DcGPX8</i>) and TCA cycle genes (<i>DcACLA</i> and <i>DcACLB</i>). The m<sup>6</sup>A levels of these genes were positively correlated with their mRNA expression levels. Furthermore, we found that silencing these genes significantly accelerates carnation petal senescence and increases oxidative stress, which highlights the key role of m<sup>6</sup>A modification in modulating antioxidant and metabolic pathways. This study confirms the dynamic changes and potential mechanisms of m<sup>6</sup>A modification during carnation petal senescence, providing new insights into the epigenetic regulatory network during plant senescence.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The rice heat shock transcription factor OsHSFC1b increases seed weight, size, and vigor, but its function is disrupted by isoaspartyl modification","authors":"Rakesh Kumar Achary, Nitin Uttam Kamble, Shikha Gautam, Abhijit Hazra, Vishal Varshney, Shivangi Mahawar, Saroj Laha, Manoj Majee","doi":"10.1111/tpj.70365","DOIUrl":"https://doi.org/10.1111/tpj.70365","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant optimizes seed size, weight, vigor, and various other features during seed development, which are important not only for their successful propagation and establishment but also for effective agriculture. Despite several studies conducted, understanding how plants coordinate the regulatory mechanisms to achieve optimal seed size, weight, and vigor remains elusive. Here, our study reveals the role of rice heat shock transcription factor OsHSFC1b in modulating various seed attributes. We observe that <i>OsHSFC1b</i> expression increases during the later stage of seed development and is primarily localized in the embryo. We found that <i>hsfc1b</i> genome-edited lines exhibit compromised seed size, weight, and vigor, while overexpression lines exhibit increased seed size, weight, and vigor compared with the wild-type seeds. Our study further reveals that OsHSFC1b improves seed vigor by activating HSPs and RFO biosynthetic genes involved in protection mechanisms, while also mediating seed size and weight by modulating auxin biosynthesis, endosperm development, and seed filling. We found that upon ageing and stressful environments, OsHSFC1b undergoes isoaspartyl modification that negatively impacts its biological function in seeds. Our MS/MS analyses confirm that asparagine residues near the DNA-binding domain and nuclear localization sequence of OsHSFC1b undergo isoaspartyl modification that adversely affects OsHSFC1b's transactivation activity. However, PROTEIN L-ISOASPARTYL METHYLTRANSFERASE interacts and repairs this isoaspartate-mediated damage, and restores the function of OsHSFC1b. Taken together, our study uncovers how isoaspartyl modification affects the transactivation ability of OsHSFC1b, yet the intervention of PIMT not only repairs this damage but also elevates agronomically important seed traits.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A ray localized vacuolar sucrose transport is required for wood formation in Populus tomentosa","authors":"Jing Lu, Yanhong Wang, Yaqi Wen, Qiao Ren, Qilin Wang, Xianqiang Wang, Chunzhao Liu, Qingwei Zhang, Keming Luo","doi":"10.1111/tpj.70347","DOIUrl":"https://doi.org/10.1111/tpj.70347","url":null,"abstract":"<div>\u0000 \u0000 <p>Wood development in perennial trees is a carbon demanding process essential for secondary cell wall formation and xylem development. Ray tissues are critical for organizing the radial transport system, but the regulatory mechanisms behind this transport and its influence on wood development remain largely unexplored. SUGAR WILL EVENTUALLY BE EXPORTED TRANSPORTERs (SWEETs) are key players in facilitating carbohydrate transport into the sink, yet their role in radial sugar transport from phloem to developing wood is unclear. In this study, we show that <i>PtoSWEET17b</i> is preferentially expressed in ray cells of <i>Populus tomentosa</i> secondary xylem, localizing to the vacuolar membrane. Functional analysis in yeast demonstrated that <i>PtoSWEET17b</i> functions as a high-capacity sucrose transporter. We demonstrated that the knockout of <i>PtoSWEET17b</i> resulted in reduced <sup>13</sup>C incorporation into xylem and lower soluble sugar content in wood; this is accompanied by a decrease in xylem formation and a decrease in cellulose and hemicellulose levels. Conversely, overexpression of <i>PtoSWEET17b</i> increased xylem production and soluble sugar content compared with wild-type plants. Collectively, these results establish <i>PtoSWEET17b</i>'s role in regulating radial transport from ray tissues to xylem, providing insights into post-phloem sugar transport in developing <i>Populus</i> stems.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}