环境•农林最新文献

{"title":"Phenotypic and functional trait variation in Malvastrum coromandelianum under different tree plantations in Pakistan.","authors":"Ashraaf Zahra, Farooq Ahmad, Ansa Asghar, Ummar Iqbal, Jazab Shafqat, Ahmed Raza, Khawaja Shafique Ahmad","doi":"10.1007/s00114-025-02003-0","DOIUrl":"10.1007/s00114-025-02003-0","url":null,"abstract":"<p><p>Malvastrum coromandelianum (L.) Garcke (Malvaceae) is a widely distributed herbaceous species known for its ecological adaptability and invasive behavior in tropical and subtropical ecosystems. In plantation forests, such invasive understory species can alter community composition, affect regeneration, and influence ecosystem functioning. Understanding their trait plasticity across contrasting tree plantation environments is critical for managing understory biodiversity and assessing the ecological consequences of afforestation practices. This study examined the phenotypic and functional responses of M. coromandelianum across different tree plantations in Pakistan, focusing on how canopy-driven microclimatic variation and soil physicochemical conditions influence its morphological, physiological, and anatomical traits. Results revealed that functional trait responses were highly variable and plantation-specific. Plant height, shoot length, and glycine betaine accumulation were highest under Morus nigra, corresponding with elevated soil phosphorus and calcium, while the longest roots and highest proline levels occurred under Melia azedarach, where soil calcium and magnesium were also enriched. The smallest individuals were observed under Tecomella undulata, where higher sodium and lower nutrient availability may have constrained growth. Ion concentrations in plant tissues varied significantly across plantation types, generally aligning with soil ion availability-for instance, elevated shoot and root sodium under Melia azedarach corresponded with higher soil salinity, while calcium accumulation was greatest in soils with higher calcium. Leaf anatomical traits, including thicker mesophyll and midrib tissues under Morus nigra, suggested resource-acquisitive strategies, while root epidermis and stem sclerenchyma thickening under Conocarpus lancifolius reflected stress-tolerant adaptations. Structural traits varied along the Leaf-Stem-Root Economics Spectrum (LSRES), indicating a balance between conservative and acquisitive strategies depending on resource availability. These findings highlight the species considerable plasticity in response to integrated canopy and soil factors. However, given its invasive nature, M. coromandelianum should not be promoted for use in ecological restoration without careful consideration of its ecological impacts. Instead, its consistent trait responses make it a potentially valuable bioindicator for assessing understory microhabitat conditions, especially in semi-arid plantations.</p>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"112 4","pages":"52"},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced immobilization of sorbed Pb and Cr during the Fe(II)-catalyzed transformation of ferrihydrite","authors":"Zhongkuan Wu, Chaojun Wang, Bruno Lanson, Wenbo Dong, Jiarui Jian, Feiting Yu, BinBin Wang, Haowen Zou, Bo Chen, Rui Sun, Feng He","doi":"10.1039/d5en00241a","DOIUrl":"https://doi.org/10.1039/d5en00241a","url":null,"abstract":"The secondary mineralization of metastable iron (oxyhydr)oxides into thermodynamically stable ones occurring in soil–water interface and sediment mediates the transport of pre-associated trace metal elements (and of other pollutants) in surficial environments. However, the fate of these associated metals and their impact during/on this mineralogical process remain incompletely understood. To address this question, we investigated the transformation of typical trace metal cation [Pb(<small>II</small>)] or anion [Cr(<small>VI</small>)O<small>₄</small><small>²⁻</small>] sorbed nanoscale ferrihydrite upon exposure to aqueous Fe(<small>II</small>). After 14 days of interaction, pre-sorbed Pb(<small>II</small>) modified marginally the extent of ferrihydrite conversion (∼93% <em>vs.</em> ∼94%), but did retard the formation of goethite (∼9% <em>vs.</em> ∼29%) by incorporating it into intermediates. In contrast, the transformation of Cr(<small>VI</small>)O<small>₄</small><small>²⁻</small> pre-sorbed ferrihydrite was essentially hindered (∼11%) owing to the formation of (FeO)<small>₂</small>CrO<small>₂</small><small>⁻</small> complex and less conductive Fe<small>_<em>x</em></small>Cr<small>_{1−<em>x</em>}</small>(OH)<small>₃</small> at the ferrihydrite surface. In all cases, the secondary mineralization of ferrihydrite significantly enhanced immobilization of sorbed metals (Pb/Cr release rate constant decreased by 99%/37%) owing to the formation of more stable iron (oxyhydr)oxides (<em>i.e.</em>, hematite, goethite, and lepidocrocite) partially incorporating pre-sorbed Pb(<small>II</small>) (&gt;24%), or to the formation of Fe<small>_<em>x</em></small>Cr<small>_{1−<em>x</em>}</small>(OH)<small>₃</small> and Cr<small>₂</small>O<small>₃</small> that are more resistant to acid corrosion than sorbed species. These findings offer valuable insights into the fate of typical trace metal elements during ferrihydrite transformation and contribute to the development of remediation strategies for metal contaminants.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"13 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of copper ions and their combination with nanoplastics on the aerobic denitrifying bacterium Pseudomonas stutzeri: denitrification performance, physiological and biochemical responses, and transcriptomic changes","authors":"Ze-yu Chen, Jian-wei Qu, Wei-le Meng, Miao-yi Tang, Xiao-wei Xu, Yue Zhi, You-peng Chen, Peng Yan, Fang Fang, Jin-song Guo","doi":"10.1039/d5en00310e","DOIUrl":"https://doi.org/10.1039/d5en00310e","url":null,"abstract":"Aerobic denitrifying bacteria are effective for removing N from wastewater during treatment. However, Cu can disrupt enzyme activity and damage cell structures, thereby inhibiting this denitrification process. Nanoplastics (NPs) adsorbed with Cu may mitigate the inhibitory effects of Cu, although their impact on bacteria can vary based on the properties of NPs, such as surface charge. This study investigated the effects of Cu and two types of polystyrene NPs (PS NPs and PS-NH<small>₂</small> NPs) on the growth, N removal performance, and physiological responses of the aerobic denitrifying bacterium <em>Pseudomonas stutzeri</em>. The results revealed that exposure to 10 mg L<small>⁻¹</small> Cu reduced <em>P. stutzeri</em> growth by 83.2%, significantly inhibited nitrate removal and nitrate reductase activity (<em>p</em> &lt; 0.05), and increased reactive oxygen species (ROS) levels by 115.5%. The presence of 50 mg L<small>⁻¹</small> PS NPs partially mitigated the growth inhibition from Cu (∼5%). However, 50 mg L<small>⁻¹</small> PS-NH<small>₂</small> NPs did not alleviate this inhibitory effect of Cu. Under Cu stress, N metabolism-related genes were downregulated (FC &lt; 0.5), leading to a 27% decrease in N removal performance. Conversely, genes associated with energy metabolism, Cu resistance, extracellular polymeric substance (EPS) synthesis, biofilm formation, and reactive oxygen species (ROS) scavenging were upregulated (FC &gt; 2), thereby enhancing strain ability to withstand Cu stress. Co-exposure to both Cu and PS NPs increased the expression of N metabolism-related genes (FC &gt; 2), which improved N removal efficiency by 46%. This improvement can be attributed to the ability of PS NPs to adsorb some Cu and enhance EPS secretion by <em>P. stutzeri</em>, thereby reducing Cu influx. These findings provide new insights into the impacts of Cu and NP stress on aerobic denitrifying bacterial growth in natural environments.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"14 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Social capital as a policy catalyst: Enhancing municipal investment in climate risk reduction and adaptation","authors":"Pablo Neudörfer ,&nbsp;Patricio Valdivieso","doi":"10.1016/j.gloenvcha.2025.103041","DOIUrl":"10.1016/j.gloenvcha.2025.103041","url":null,"abstract":"<div><div>Does social capital guide municipalities toward improved investment in disaster risk reduction and climate change adaptation? This article investigates whether communities with high vulnerability to climate change risks and strong social capital traits are more likely to invest in preparedness for climate change disasters. We leverage the exogenous variation of local downpours in Chile from 2009 to 2018 as a catalyst for increased awareness to examine the effects of social capital on public investment in adaptation. Our findings reveal that linking and bridging social capital levels affect preparedness as communities experience more downpours, while bonding social capital levels have no effect. These results suggest that linking and bridging could steer policymakers toward increased investment, contributing to more resilient and sustainable communities in the face of climate change. Furthermore, social capital may have the potential to shift policymakers’ focus from popular short-term disaster relief spending to long-term preparedness investments.</div></div>","PeriodicalId":328,"journal":{"name":"Global Environmental Change","volume":"94 ","pages":"Article 103041"},"PeriodicalIF":8.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Projected land-use change emissions surpass climate change-induced carbon sinks in Sub-Saharan African biomes","authors":"Dabwiso Sakala,&nbsp;Maria J. Santos","doi":"10.1016/j.gloenvcha.2025.103039","DOIUrl":"10.1016/j.gloenvcha.2025.103039","url":null,"abstract":"<div><div>Land-use and land-cover change (LULCC) and climate change (CC) effects on carbon dynamics in Sub-Saharan Africa (SSA) have the potential to transition the region’s carbon balance from sink to source. Future shared socioeconomic pathways (SSPs) for SSA show high vulnerability to CC, high urbanisation, and cropland and pasture demands expected to rise in the coming decades. Yet the realisations of these pathways may be biome-specific as these are expected to behave differently when it comes to carbon dynamics and also be affected by different demands. Here, we examine the impacts of LULCC and CC on Net Ecosystem Exchange (NEE), Net Primary Productivity (NPP), and carbon turnover time of six SSA biomes, namely tropical rainforest, montane forest, moist savanna, dry savanna, temperate grassland and semi-desert, between 2015–2100 for the five SSPs. We performed four simulations where we (i) kept LULCC and CC constant, (ii) varied LULCC and kept CC constant, (iii) kept LULCC constant and varied CC, and (iv) varied both LULCC and CC and calculated their effect on NEE, NPP and carbon turnover time. We find that LULCC effects supersede those of CC, resulting in a net carbon source despite the sink effect of CC. LULCC alone drives major carbon losses across all scenarios, with moist and dry savannas resulting in the strongest carbon sources with cumulative NEE values of 74.93 and 44.85 PgC by 2100 under SSP4. In contrast, CC alone led to consistent carbon sinks across all scenarios in the semi-desert and tropical rainforest, with the strongest sinks observed under SSP5 (−8.36 PgC and − 3.77 PgC, respectively). Moist savanna also acted as a sink under SSP5 (−4.82 PgC) and SSP3 (−1.73 PgC). Combined effects largely reflect LULCC trends, confirming its dominant role in shaping future carbon dynamics. We further observe that intensifying LULCC may decrease biome carbon turnover time by 50 %, especially in tropical rainforests under SSP3 and SSP4. These results suggest that the CC-induced carbon sinks will unlikely dampen the increasing emissions from LULCC in SSA that may not be offset by 2100. Ultimately, our study demonstrates that sustaining SSA as a carbon sink will require urgent, biome-specific land management policies that integrate socioeconomic realities with the region’s evolving climate and development pathways.</div></div>","PeriodicalId":328,"journal":{"name":"Global Environmental Change","volume":"94 ","pages":"Article 103039"},"PeriodicalIF":8.6,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-specific Responses of Duckweed to Cadmium Stress under Nanoplastic Co-exposure: Differential Accumulation and Toxicity in Roots and Fronds","authors":"Zhihao Xue, Xinyi Liao, Jie Hou, Jiang Xu, Daohui Lin","doi":"10.1039/d5en00432b","DOIUrl":"https://doi.org/10.1039/d5en00432b","url":null,"abstract":"Nanoplastics (NPs), characterized by their small size and widespread presence, are known to interact with other contaminants, potentially modifying their environmental behavior and biological impacts. Herein, we explored the response of duckweed (Spirodela polyrhiza) to Cd (0, 0.01, 0.1, 1 mg/L) stress upon co-exposure to polystyrene NPs (PS-NPs) (0, 0.1, 1, 10 mg/L). Cd exhibits significantly higher toxicity to the duckweed compared to PS-NPs. Notably, the presence of PS-NPs exacerbates Cd toxicity in the roots, while mitigating its adverse effects on the fronds. In-situ analyses using Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Confocal Laser Scanning Microscopy demonstrate that PS-NPs enhance Cd accumulation in the root tips (by up to 19.3%) but reduce its presence in the basal root regions and further translocation to the fronds by up to 26.8%. Metabolomics and gene expression analyses further indicate that PS-NPs elevate organic acid contents and modify gene expressions in duckweed against exogenous Cd stress, thereby inhibiting the root-to-frond translocation of Cd. The findings enhance our comprehension of the complex interactions between NPs and environmental pollutants in aquatic flora.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"108 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nano-crystalline Hf(IV) MOF for precise fluorometric recognition of carcinogenic nicotine and herbicide benfluralin: characterization, real-Life application and mechanistic investigation","authors":"Srijan Mukherjee, Sandip Roy, Pitam Chakrabarti, Vishal Trivedi, Shyam Biswas","doi":"10.1039/d5en00141b","DOIUrl":"https://doi.org/10.1039/d5en00141b","url":null,"abstract":"Metal-organic frameworks (MOFs) are emergent smart materials with significant potential in rapid fluorescence-based recognition of toxic organic pollutants. Nicotine (NIC), an extensively consumed neurotetratogenic alkaloid with addictive properties, and benfluralin, a broadly used herbicide, are both potent toxic organic pollutants with several serious health impacts. Extensive exposure of NIC and benfuralin in the environment poses an imperative demand for the development of a reliable sensor for dual recognition of NIC and benfluralin and mitigating the release of toxic substances. Therefore, we have constructed a robust nano-crystalline Hf-based MOF, 1ʹ for fluorescence-based aqueous phase turn-on detection of NIC, in addition to turn-off-based recognition of benfluralin in MeOH medium. Fluorogenic nano-crystalline sensor 1ʹ selectively detects both the targeted analytes with higher precision, even in nanomolar concentration. Moreover, the extended investigation was conducted using the probe 1ʹ for quantitative detection and monitoring of NIC in biofluids, real wastewater specimens and tobacco leaf extracts. Additionally, the capability of 1ʹ in recognition of NIC in live MDAMB-231 breast cancer cells was demonstrated through cell imaging. Furthermore, a biocompatible, cost-effective 1ʹ@chitosan@paper composite strip was demonstrated for instantaneous detection of NIC and benfluralin through a visible change in fluorescence response. Furthermore, a detailed mechanistic investigation was performed to validate a plausible mechanistic pathway for the observed optical response of 1ʹ in the presence of both NIC and benfluralin. This work offers a new approach to precisely monitor and reduce the release of toxic NIC and benfluralin, thereby contributing in safeguarding public health and the environment.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"48 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering decolonial pedagogies for learning agroecological transitions: comparative analysis of South America cases","authors":"Michelle Bonatti ,&nbsp;Renata Guimarães Reynaldo ,&nbsp;Berta Martín-López ,&nbsp;Sergio Bolivar ,&nbsp;María Cordero-Fernández ,&nbsp;Giovanna Chavez Miguel ,&nbsp;Adriana Martin ,&nbsp;Janika Hämmerle ,&nbsp;Barbara Schröter ,&nbsp;Carla Erismann ,&nbsp;Teresa da Silva Rosa ,&nbsp;Jon Hellin ,&nbsp;Izabella Schlindwein ,&nbsp;Álvaro Acevedo Osorio ,&nbsp;Leonardo Medina ,&nbsp;Carla Baldivieso ,&nbsp;Luca Eufemia ,&nbsp;Johanna Jacobi ,&nbsp;Ana Maria Lobo Guerrero ,&nbsp;Stefan Sieber","doi":"10.1016/j.gloenvcha.2025.103042","DOIUrl":"10.1016/j.gloenvcha.2025.103042","url":null,"abstract":"<div><div>Agroecological transitions represent strategic pathways for transforming agricultural systems to meet urgent global sustainability goals. These transitions encompass fundamental changes in social-ecological relationships, knowledge systems, and power dynamics within food systems. However, the mechanisms facilitating such transitions remain insufficiently understood, particularly regarding the diversity and efficacy of pedagogical models employed in existing agroecological initiatives. This knowledge gap is especially pronounced within Global South contexts, especially Latin America, where decolonial approaches hold particular relevance and tradition within effective agroecology initiatives. Using a decolonial lens, this study explores the pedagogical models used in community-led agroecological initiatives in Brazil, Colombia, and Peru. Drawing on semi-structured interviews and workshops (n 140), alongside participant observations, we applied a qualitative archetypes analysis to examine three community-led agroecology initiatives. We identified three distinct but interconnected contextual narratives: a. Living Pedagogies; b. Resistance Pedagogies; and c. Hybrid Pedagogies. Despite these different contextual narratives, they share clear patterns, which allowed us to identify one major archetype − the South pedagogies archetype. The decolonial pedagogy found can be fundamental to accelerate agroecological transitions. Traditional communities in Colombia and Peru have preserved and evolved their agroecology knowledge systems through generations of collective learning, offering profound insights into sustainable food production that transcend the limitations of Western scientific methodologies. In parallel, decolonial pedagogies in the Brazilian case were essential to promote urban agroecological transition that started during the 2000s. These findings inform agroecological transition development based on learning processes that value multiple ways of being.</div></div>","PeriodicalId":328,"journal":{"name":"Global Environmental Change","volume":"94 ","pages":"Article 103042"},"PeriodicalIF":8.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface-Enhanced Raman Spectroscopy for Size-Resolved Microplastic Detection in Real-world Samples Using Thiophenol Labeling","authors":"Jayasree Kumar, Arunima Jinachandran, Mounika Renduchintala, Venugopal Rao Soma, Vijayakumar Shanmugam, Imam Shaik, Sreenivasulu Tupakula, Rajapandiyan Panneerselvam","doi":"10.1039/d5en00211g","DOIUrl":"https://doi.org/10.1039/d5en00211g","url":null,"abstract":"The widespread presence of plastic contamination in the environment presents a severe threat to human and animal health. This study introduces a toluene dispersion strategy for detecting microplastics of different sizes using surface-enhanced Raman spectroscopy (SERS). The evaporation-induced self-assembly (EISA) method was employed to prepare SERS substrates by incubating silver nanoparticles (AgNPs) of ~40-60 nm with a microplastic solution containing polystyrene (250 μm, 2.1 mm), polypropylene (10-50 μm), and polyvinyl chloride (1-5 μm). SEM images and Raman spectroscopy confirmed the uniform decoration of AgNPs on filter paper substrates, with a relative standard deviation (RSD) of 8.22%. Thiophenol was used as a Raman reporter to monitor surface changes, showing a strong correlation (R² = 0.986-0.995) between its SERS signal and microplastic concentration in aqueous and real samples. This is the first time a toluene dispersion strategy has been integrated with EISA to achieve highly sensitive microplastic detection, reaching a limit of 0.001 mg/mL. The method was validated in real-world matrices, including lake water and salt samples, in the presence of interferences like organic pollutants, inorganic ions, colloids, bio-organisms, and bisphenol A. This approach enables rapid detection of diverse microplastics in complex environmental samples.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"39 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell mass cytometry reveals cell type- and cluster-specific heterogeneity in silver nanoparticle responses in a 3D alveolar tetra-culture model","authors":"Eunseo Lee, Seung-Geun Park, Seung Min Ha, Minseop Kim, Sehee Park, Aline Chary, Tommaso Serchi, Tae Hyun Yoon","doi":"10.1039/d5en00439j","DOIUrl":"https://doi.org/10.1039/d5en00439j","url":null,"abstract":"Silver nanoparticles (AgNPs) are widely used in medicine, environmental science, and industry. However, their heterogeneous interactions with complex biological systems, especially at the single-cell level, are not fully understood yet. Conventional toxicity assessment methods are typically conducted on oversimplified in vitro models that fail to replicate actual physiological conditions, and measure collective responses across cell populations, obscuring differences among individual cells. To overcome these limitations, we utilized single-cell mass cytometry (CyTOF) to investigate individual cell responses to AgNP-induced stress, combined with a 3D alveolar tetra-culture model designed to better reflect the complexity of biological systems. Single-cell mass cytometry of a 3D alveolar model revealed heterogeneous, cell type–specific responses to AgNP exposure. Specifically, PMA-differentiated THP-1, A549 and EA.hy926 cells exhibited high AgNP association but limited cytotoxicity, indicating activation of stress-mitigation pathways, while THP-1 cells showed early inflammatory activation despite minimal AgNP association, suggesting indirect mechanism. Single-cell analysis and FlowSOM clustering revealed distinct subpopulations exhibiting diverse intracellular signaling profiles of inflammatory cytokines, anti-inflammatory mediators, and stress-response proteins, which unveiled common cellular responses and unique cell-type specific pathways determining cell fate (survival, transitional states, or apoptosis) upon AgNP exposure. This study introduces a novel framework for studying heterogeneous interactions of nanoparticles with complex biological system by integrating 3D alveolar tetra-culture model with single-cell mass cytometry analysis, enabling the dissection of nanoparticle-induced stress responses at an unprecedented level of detail. These insights have broad implications for nanotoxicology and nanomedicine, underscoring the need to account for cellular heterogeneity when evaluating nanoparticle-induced toxicity.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"14 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}