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Applying the Three Horizons approach in local and regional scenarios to support policy coherence in SDG implementation: Insights from arid Spain 在地方和区域方案中应用 "三个地平线 "方法,支持可持续发展目标实施工作中的政策一致性:西班牙干旱地区的启示
IF 8.6 1区 环境科学与生态学
Global Environmental Change Pub Date : 2024-09-09 DOI: 10.1016/j.gloenvcha.2024.102922
{"title":"Applying the Three Horizons approach in local and regional scenarios to support policy coherence in SDG implementation: Insights from arid Spain","authors":"","doi":"10.1016/j.gloenvcha.2024.102922","DOIUrl":"10.1016/j.gloenvcha.2024.102922","url":null,"abstract":"<div><p>The Three Horizons for the Sustainable Development Goals (SDGs) is a novel participatory approach to co-create future sustainable scenarios for supporting the implementation of the United Nations 2030 Agenda. Whereas the approach has been applied to inform the design of global-scale sustainability scenarios based on regional perspectives, it has not been implemented to explore how local and regional scenarios can be connected across sites and scales to inform governance processes in the implementation of the SDGs. This study applies an adapted version of the Three Horizons for the SDGs approach in four sites at regional and local scales in Spanish drylands to explore its potential to support policy coherence at multiple governance scales for advancing SDG implementation through dialogue between actors from multiple sectors. We conducted four two-day in-person workshops with diverse actors (n = 59) to explore their perceptions about the desired futures, current concerns, and strategies to achieve sustainable futures in the region. Results reveal 27 similar and nine dissimilar themes related to desired futures and current concerns, respectively. These findings provide common ground and highlight different contextual realities between sites that may serve as a basis for harmonizing policy priorities for advancing regional and local SDG implementation. The study also identifies 19 themes encompassing multiple strategies with the potential to establish associations across sites and scales to coordinate actions in alignment with the 2030 Agenda. We argue that the adapted version of the Three Horizons for the SDGs approach can serve as a tool to support coherent multi-scale governance needed to achieve global sustainability goals. We discuss lessons learned and limitations encountered from using the approach that provides guidance for future experiences.</p></div>","PeriodicalId":328,"journal":{"name":"Global Environmental Change","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Silica nanoparticles enhance wheat resistance to fusarium head blight through modulating antioxidant enzyme activities and salicylic acid accumulation 纳米二氧化硅颗粒通过调节抗氧化酶活性和水杨酸积累增强小麦对镰刀菌头枯病的抗性
IF 8.131 2区 环境科学与生态学
Environmental Science: Nano Pub Date : 2024-09-09 DOI: 10.1039/d4en00435c
Junliang Yin, Keke Li, Xi Liu, Shuo Han, Xiaowen Han, Wei Liu, Yiting Li, Yunfeng Chen, Yongxing Zhu
{"title":"Silica nanoparticles enhance wheat resistance to fusarium head blight through modulating antioxidant enzyme activities and salicylic acid accumulation","authors":"Junliang Yin, Keke Li, Xi Liu, Shuo Han, Xiaowen Han, Wei Liu, Yiting Li, Yunfeng Chen, Yongxing Zhu","doi":"10.1039/d4en00435c","DOIUrl":"https://doi.org/10.1039/d4en00435c","url":null,"abstract":"Fusarium head blight (FHB) disease severely impacts wheat production and quality. Silica nanoparticles (SiNPs) are demonstrated as an eco-friendly option for diseases management, but the specific mechanisms behind their ability to confer disease resistance in wheat have not been adequately characterized. This study evaluates the impact of SiNP200 on the resistance of wheat to FHB. Scanning electron microscope observation showed that SiNPs form a physical barrier on the surface of wheat leaves. Pathogenicity tests indicated foliar application of SiNP200 can protect wheat against F. graminearum, resulting in a significant reduction of lesion length by 27.7%, but in-vitro cultivation showed that SiNP200 had no impact on pathogen growth. Antioxidant enzyme activity analysis showed that SiNP200 had little effect on H2O2 contents, POD, and CAT activities under non-stress conditions, but under F. graminearum infection conditions, SiNP200 increased POD and SOD activities while decreased CAT and DHAR activities, and GSH content. Histochemical staining indicated that SiNP200 decreased ROS accumulation, thus reducing oxidative damage. Meanwhile, SiNP200 decreased MDA and Pro contents. Furthermore, SiNP200 increased SA response marker genes (TaPR1a, TaPR2 and TaPR5) expression levels and SA content, contributing to the enhanced wheat resistance to FHB. Summarily, SiNP200 improve wheat resistance to FHB, thereby providing a theoretical basis for SiNP200 application to control this disease.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":8.131,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding the role of biomolecular coronas in human exposure to nanomaterials 了解生物分子冠层在人类接触纳米材料中的作用
IF 8.131 2区 环境科学与生态学
Environmental Science: Nano Pub Date : 2024-09-09 DOI: 10.1039/d4en00488d
Mahmoud G. Soliman, Alberto Martinez-Serra, Giulia Antonello, Marko Dobricic, Terence Wilkins, Tommaso Serchi, Ivana Fenoglio, Marco P. Monopoli
{"title":"Understanding the role of biomolecular coronas in human exposure to nanomaterials","authors":"Mahmoud G. Soliman, Alberto Martinez-Serra, Giulia Antonello, Marko Dobricic, Terence Wilkins, Tommaso Serchi, Ivana Fenoglio, Marco P. Monopoli","doi":"10.1039/d4en00488d","DOIUrl":"https://doi.org/10.1039/d4en00488d","url":null,"abstract":"Nanomaterials (NMs) are increasingly used in medical treatments, electronics, and food additives. However, nanosafety—the possible adverse effects of NMs on human health—is an area of active research. This review provides an overview of the influence of biomolecular coronas on NM transformation following various exposure routes. We discuss potential exposure pathways, including inhalation and ingestion, describing the physiology of exposure routes and emphasising the relevance of coronas in these environments. Additionally, we review other routes to NM exposure, such as synovial fluid, blood (translocation and injection), dermal and ocular exposure, as well as the dose and medium impact on NM interactions. We emphasize the need for an in-depth characterisation of coronas in different biological media, highlighting the need and opportunity to study lung and gastric fluids to understand NM behaviour and potential toxicity. Future research aims to predict better <em>in vivo</em> outcomes and address the complexities of NM interactions with biological systems.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":8.131,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142160529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mesoporous Activated Carbon from Sunflower (Helianthus annuus) Seed Pericarp for Crystal Violet Dye Removal: Numerical Desirability Optimization and Mechanism Study 用于去除水晶紫染料的向日葵(Helianthus annuus)种子果皮介孔活性炭:数值可取性优化和机理研究
IF 2.52 4区 环境科学与生态学
Water, Air, & Soil Pollution Pub Date : 2024-09-07 DOI: 10.1007/s11270-024-07477-8
Ali H. Jawad, Nursuhada Salleh, Zeid A. ALOthman, Rangabhashiyam Selvasembian
{"title":"Mesoporous Activated Carbon from Sunflower (Helianthus annuus) Seed Pericarp for Crystal Violet Dye Removal: Numerical Desirability Optimization and Mechanism Study","authors":"Ali H. Jawad, Nursuhada Salleh, Zeid A. ALOthman, Rangabhashiyam Selvasembian","doi":"10.1007/s11270-024-07477-8","DOIUrl":"https://doi.org/10.1007/s11270-024-07477-8","url":null,"abstract":"<p>In this work, sunflower (<i>Helianthus annuus</i>) seed pericarp (SSPC) was converted into mesoporous activated carbon (SSPCAC) <i>via</i> microwave-assisted ZnCl<sub>2</sub> activation to be a cost-effective and renewable adsorbent for crystal violet (CV) dye removal. The obtained SSPCAC exhibits a preferable surface area of 641 m<sup>2</sup>/g with mesoporous characteristics. A statistical optimization by Box-Behnken Design (BBD) with Response Surface Methodology (RSM) was adopted to achieve the optimal operational conditions for CV dye including SSPCAC dose, solution pH, and contact time. Moreover, desirability functions confirm the maximum CV removal of 91% was attended at SSPC-AC dose (0.083 g/100 mL), solution pH (9.8), and contact time (5.38 min). Thus, the equilibrium data were best described by the Langmuir isotherm model with a maximum adsorption capacity of 111.9 mg/g at 25 <sup>0</sup>C. Thus, the adsorption kinetics were well described by a pseudo-second order (PSO) model. The adsorption mechanism of CV onto SSPCAC surface can be assigned to the electrostatic attraction, hydrogen bonding, pore filling, and pi-pi interactions. This research highlights the potential of sunflower seed pericarp as a renewable precursor for activated carbon production with promising applications in toxic dye removal from wastewater.</p>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":null,"pages":null},"PeriodicalIF":2.52,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Manganese oxide nanoparticles elicit cadmium tolerance in wheat seedlings by ionomic and phenomic adjustment, regulation of AsA–GSH pathway, cellular thickness recovery, and antioxidant modulation 纳米氧化锰颗粒通过离子组学和表观组学调整、ASA-GSH 通路调控、细胞厚度恢复和抗氧化剂调节,激发小麦幼苗的镉耐受性
IF 8.131 2区 环境科学与生态学
Environmental Science: Nano Pub Date : 2024-09-06 DOI: 10.1039/d4en00490f
Muhammad Anas, Samina Bibi, Ume Farwa, Amjid Khan, Umar Masood Quraishi
{"title":"Manganese oxide nanoparticles elicit cadmium tolerance in wheat seedlings by ionomic and phenomic adjustment, regulation of AsA–GSH pathway, cellular thickness recovery, and antioxidant modulation","authors":"Muhammad Anas, Samina Bibi, Ume Farwa, Amjid Khan, Umar Masood Quraishi","doi":"10.1039/d4en00490f","DOIUrl":"https://doi.org/10.1039/d4en00490f","url":null,"abstract":"This study evaluates the effects of cadmium (Cd) stress on wheat cultivars SKD-1 and Borlaug-16 and the use of manganese dioxide nanoparticles (MnO<small><sub>2</sub></small>NPs) for remediation. Upon characterization, MnO<small><sub>2</sub></small>NPs demonstrated colloidal stability and effectiveness, with an average size of 37.63 nm determined by the Scherrer equation and 41 nm by Rietveld refinement, flake-like shape, and a zeta potential of −10.9 mV. Both wheat cultivars were subjected to 50 mg kg<small><sup>−1</sup></small> Cd stress and treated with MnO<small><sub>2</sub></small>NPs at 100, 250, and 500 ppm through nano-priming. MnO<small><sub>2</sub></small>NPs significantly reduced Cd uptake by 20–50% in both root and leaf tissues. Cd exposure increased toxic elements like arsenic, chromium, copper, nickel, and lead in Borlaug-16 roots by up to 50% and decreased essential minerals by 30–60%. The 250 ppm MnO<small><sub>2</sub></small>NPs treatment restored essential minerals by up to 45%. In SKD-1, Cd exposure raised H<small><sub>2</sub></small>O<small><sub>2</sub></small> levels by 78.03% in roots and malondialdehyde by 40.03% in leaves. MnO<small><sub>2</sub></small>NPs reduced H<small><sub>2</sub></small>O<small><sub>2</sub></small> to 7.85 μmol g<small><sup>−1</sup></small> and malondialdehyde to 1.29 mmol g<small><sup>−1</sup></small>, while increasing superoxide dismutase activity by 86.77%. Anatomical analysis revealed that Cd exposure increased epidermis thickness by 16.80% and decreased cortex thickness by 29.10% in SKD-1 roots, while MnO<small><sub>2</sub></small>NPs promoted recovery by 50–80%. Ionomic analysis showed that MnO<small><sub>2</sub></small>NPs improved ion distribution and reduced Cd uptake by 40–60%, with notable improvements in calcium, magnesium, and potassium levels. These findings highlight the potential of MnO<small><sub>2</sub></small>NPs in mitigating Cd stress, enhancing physiological resilience, and elemental balance in wheat. Future research should focus on optimizing MnO<small><sub>2</sub></small>NPs application to achieve sustainable crop production.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":8.131,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of green financial sector initiatives in the low-carbon transition: A theory of change 绿色金融部门倡议在低碳转型中的作用:变革理论
IF 8.6 1区 环境科学与生态学
Global Environmental Change Pub Date : 2024-09-06 DOI: 10.1016/j.gloenvcha.2024.102915
{"title":"The role of green financial sector initiatives in the low-carbon transition: A theory of change","authors":"","doi":"10.1016/j.gloenvcha.2024.102915","DOIUrl":"10.1016/j.gloenvcha.2024.102915","url":null,"abstract":"<div><p>Green financial sector initiatives, including green macroprudential policies, green monetary policies, and green public co-funding, could play an important role in the low-carbon transition by supporting countries in the implementation of their climate objectives. This paper analyzes how green financial sector initiatives could enable the scaling up of green investments while avoiding unintended effects on macroeconomic and financial stability. For each green financial sector initiative, the paper identifies its entry point in the economy, the transmission channels to banks’ investment decisions in terms of availability and cost of capital for high- and low-carbon goods, and the resulting impacts on output and greenhouse gas emissions. Building on these insights, the paper develops a theory of change about the role of green financial sector initiatives for climate mitigation, identifying the criteria for applicability and conditions to maximize their impact. It discusses specifically the application of the theory of change to the low-carbon transition in coal and carbon intensive regions in the context of the European net zero climate objective.</p></div>","PeriodicalId":328,"journal":{"name":"Global Environmental Change","volume":null,"pages":null},"PeriodicalIF":8.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coupling of Biostimulation and Bioaugmentation for Benzene, Toluene, and Trichloroethylene Removal from Co-Contaminated Soil 耦合生物刺激和生物增效,去除共污染土壤中的苯、甲苯和三氯乙烯
IF 2.52 4区 环境科学与生态学
Water, Air, & Soil Pollution Pub Date : 2024-09-05 DOI: 10.1007/s11270-024-07481-y
Huinan Liu, Runmin Wang, Moye Luo, Chenghua Xu, Dandan Yu, Manjun Zhan, Tao Long, Ran Yu
{"title":"Coupling of Biostimulation and Bioaugmentation for Benzene, Toluene, and Trichloroethylene Removal from Co-Contaminated Soil","authors":"Huinan Liu, Runmin Wang, Moye Luo, Chenghua Xu, Dandan Yu, Manjun Zhan, Tao Long, Ran Yu","doi":"10.1007/s11270-024-07481-y","DOIUrl":"https://doi.org/10.1007/s11270-024-07481-y","url":null,"abstract":"<p>The coexistence of various organic pollutants in soil always draws extensive attention because of their difficulties and complexity for remediation. Especially, the impacts of bioremediation on soil co-contaminated with benzene, toluene, and trichloroethylene (TCE) have seldom been comprehensively evaluated yet. In this study, the contributions of biostimulation, bioaugmentation, and their combination for the bioremediation of the co-contaminated soil containing benzene, toluene, and TCE were systematically investigated. The addition of nutrients ((NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> (as N source), K<sub>2</sub>HPO<sub>4</sub> (as P source), vegetable oil and CH<sub>3</sub>COONa (as C sources)) enhanced the degradation efficiency of the co-contaminated soil by 10.19% to 49.62%. The optimal biostimulation condition involved using vegetable oil as the carbon source with a C: N: P ratio of 100: 10: 1. Meanwhile, the addition of the microbial cultures screened and domesticated from the co-contaminated soil, named B-T, effectively enhanced the removal rate of contaminants by 33.02% to 37.55%. The genera comprising <i>Pseudomonas</i>, <i>Stenotrophomonas</i>, and <i>Chryseobacterium</i> in B-T exhibited the highest relative abundance, suggesting their potential for the removal of benzene, toluene, and TCE. Besides, the coupling of biostimulation and bioaugmentation enhanced the degradation efficiency by 62.38% to 68.84%, showing the most effective biodegradation effects. The coupled strategy showed synergistic effects of both, increasing the quantity and activity of microorganisms and accelerating the biodegradation of target contaminants. The findings indicated that the coupling of bioaugmentation and biostimulation treatment strategy holds promise for the bioremediation of benzene, toluene, and TCE from co-contaminated soil.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":null,"pages":null},"PeriodicalIF":2.52,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142225779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prediction of Groundwater Quality Index and Identification of Key Variables Using Bayesian Neural Network 利用贝叶斯神经网络预测地下水质量指数并确定关键变量
IF 2.52 4区 环境科学与生态学
Water, Air, & Soil Pollution Pub Date : 2024-09-05 DOI: 10.1007/s11270-024-07459-w
Saumen Maiti, Surabhi Gupta, Praveen Kumar Gupta
{"title":"Prediction of Groundwater Quality Index and Identification of Key Variables Using Bayesian Neural Network","authors":"Saumen Maiti, Surabhi Gupta, Praveen Kumar Gupta","doi":"10.1007/s11270-024-07459-w","DOIUrl":"https://doi.org/10.1007/s11270-024-07459-w","url":null,"abstract":"<p>Prediction of groundwater quality index (GWQI) and quantification of the influence of each key water quality variable is vital for water resource management. This paper shows the potential of the automatic relevance determination-based Bayesian neural network(ARD-BNN) technique for GWQI prediction and compares the predictive performance of ARD-BNN with artificial neural network (ANN), long short-term memory network (LSTM), convolutional neural network (CNN) and hybrid CNN-LSTM. What discriminates the present approach from the previous approach is that GWQI is simulated from the global criteria of WHO and the artificial intelligence (AI) models are trained and cross-validated in a synthetically simulated data set of 1470 examples and applied to predict GWQI using newly collected groundwater samples from an area surrounding Amarpur dolerite dyke, Dhanbad, Jharkhand (INDIA). The present analysis suggests that ARD-BNN is relatively skilful (<i>MSE</i><sub>ARD-BNN</sub> = 0.01) relative to other AI models investigated (<i>MSE</i><sub>ANN</sub> = 0.06;<i>MSE</i><sub>CNN</sub> = 1.60; <i>MSE</i><sub>LSTM</sub> = 3.85;<i>MSE</i><sub>CNN-LSTM</sub> = 8.21). The efficacy of the method and stability of results is also tested in the presence of different levels of correlated noise which suggests that the ARD-BNN model is considerably unwavering for up to 20% correlated noise; however, adding more noise (∼50% or more) degrades the results. Sensitivity analysis via ARD-BNN-based soft-pruning strategy identifies that [NO<sub>3</sub><sup>−</sup>], [SO<sub>4</sub><sup>2−</sup>],[pH],[F<sup>−</sup>]and [EC], are key water quality variables for predicting GWQI in the study area.</p>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":null,"pages":null},"PeriodicalIF":2.52,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanostructured copper electrodes - a new step in the development of microbial bioelectrochemical systems 纳米结构铜电极--开发微生物生物电化学系统的新步骤
IF 8.131 2区 环境科学与生态学
Environmental Science: Nano Pub Date : 2024-09-05 DOI: 10.1039/d4en00440j
Anastasia S. Medvedeva, Elena I. Gudkova, Aleksandra S. Titova, Anna S. Kharkova, Lyubov S. Kuznetsova, Roman N. Perchikov, Vasiliy R. Ivanov, Yegor D. Ryabkov, Anna A. Tikhonova, Ekaterina D. Fomina, Alina O. Naumova, Pavel V. Melnikov, Denis N. Butusov, Viacheslav A. Arlyapov
{"title":"Nanostructured copper electrodes - a new step in the development of microbial bioelectrochemical systems","authors":"Anastasia S. Medvedeva, Elena I. Gudkova, Aleksandra S. Titova, Anna S. Kharkova, Lyubov S. Kuznetsova, Roman N. Perchikov, Vasiliy R. Ivanov, Yegor D. Ryabkov, Anna A. Tikhonova, Ekaterina D. Fomina, Alina O. Naumova, Pavel V. Melnikov, Denis N. Butusov, Viacheslav A. Arlyapov","doi":"10.1039/d4en00440j","DOIUrl":"https://doi.org/10.1039/d4en00440j","url":null,"abstract":"This work proposes a new method for forming a biosensor based on a nanopillar copper electrode and microorganisms for rapid assessment of the biochemical oxygen demand (BOD) index. Heterogeneous electron transfer constants were obtained for water-soluble and insoluble redox-compounds, and single-mediator and dual-mediator bioelectrochemical systems were formed taking into account the obtained values. It was shown that the yeast Debaryomyces hansenii is not susceptible to the toxic effect of copper and could be effectively used with a nanostructured electrode in the ferrocene-methylene blue dual-mediator system. Paracoccus yeei bacteria are susceptible to the toxic effect of copper ions, therefore, their immobilization on the nanostructured electrode was carried out using the redox-active polymer poly(neutral red), which protects microorganisms from negative effects and allows effective conjugation of microorganisms with the surface. The developed bioelectrochemical measuring systems have high sensitivity (the lower limit of determined BOD5 values is 2.0 mg/dm3) and high correlation with the standard method for BOD measurement in surface water samples (R2 &gt; 0.98). Thus, the work demonstrated for the first time the successful use of a metal nanopillar electrode as a substrate for the biosensor, and the created biosensor systems could serve as prototypes of commercially available miniature sensors for rapid assessment of water quality. The cost of the biosensor of the developed bioelectrochemical measuring systems was estimated: for a two-mediator system based on D. hansenii microorganisms and ferrocene-methylene blue mediators, the cost of one bioelectrode was 4.28 USD, and for a system based on P. yeei microorganisms and poly(neutral red) – 3.91 USD.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":8.131,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142138418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biodegradable Nanoparticles Aid the Gut Microbial Community in Delaying Antibiotic Resistance Emergence 可生物降解纳米粒子帮助肠道微生物群落延缓抗生素耐药性的产生
IF 8.131 2区 环境科学与生态学
Environmental Science: Nano Pub Date : 2024-09-05 DOI: 10.1039/d4en00382a
Genesis Herrera, Sachin Paudel, Simone Lupini, Carlos E. Astete, Christina M Sabliov, Debora Frigi Rodrigues
{"title":"Biodegradable Nanoparticles Aid the Gut Microbial Community in Delaying Antibiotic Resistance Emergence","authors":"Genesis Herrera, Sachin Paudel, Simone Lupini, Carlos E. Astete, Christina M Sabliov, Debora Frigi Rodrigues","doi":"10.1039/d4en00382a","DOIUrl":"https://doi.org/10.1039/d4en00382a","url":null,"abstract":"The antibiotic-nanoparticle combinatorial effects on gut microbiome diversity, abundance, and antibiotic resistance remain largely unknown. In the present study, we investigated the potential impacts of biodegradable nanocarriers after one biocompatible dose that could promote sustainable treatment against enteropathogens. Enrofloxacin (Enro), a common antibiotic used in livestock, was loaded into biodegradable synthetic poly(lactic-co-glycolic) acid (PLGA) and plant-based Lignin (LIGNIN) nanoparticles. Anaerobic bioreactors containing fresh slurry collected from pig intestines were used to simulate the anaerobic gut microenvironment. Bioreactors were inoculated with empty NPs with concentrations identical to loaded nanoparticles and free Enro, PLGA (Enro) (PE), and LIGNIN(Enro) (LE) that present antimicrobial activity and non-cytotoxicity to pig intestinal cells. Slurry aliquots from the bioreactors were collected for RNA extraction after 24, 48, and 72 hours of exposure to the drug and nanocarriers for microbial 16S rRNA metatranscriptomics and resistome analysis. Our results showed that PLGA and PE microbial communities were similar over all periods despite containing Enro. The impact of LIGNIN on the microbial community was minimal since it was similar to the control. However, loaded LE significantly reduced the microbial diversity but maintained essential estimated metabolic functions. Free Enrofloxacin affected the microbial community the most by decreasing the core gut microbiome diversity. Our results indicate that NP encapsulation of antibiotics delayed the increase in antibiotic resistance genes (ARG) expression between 24 and 72 hours compared to free Enro. These results demonstrate that the antibiotic treatment conveyed by NPs slows the rate of expression of ARG and reduces the adverse antibiotic effects on the gut microbial community.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":null,"pages":null},"PeriodicalIF":8.131,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142138417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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