Areej S. Sadiq, Mohammed Y. Fattah, Mohammed F. Aswad
{"title":"Strength and Compressibility of HCl Contaminated Clayey Soil","authors":"Areej S. Sadiq, Mohammed Y. Fattah, Mohammed F. Aswad","doi":"10.1007/s11270-025-07762-0","DOIUrl":"10.1007/s11270-025-07762-0","url":null,"abstract":"<div><p>The present research seeks to comprehend and evaluate the impact of varying concentrations of HCl acid solution on the compressibility and shear strength of silty clay. The compression test and the unconfined compressive strength test were conducted, and the SEM test was performed to analyze the microstructure of the soil with and without contamination. The results indicate that the contamination of silty clay soil with HCl acid caused a reduction in strength and an increase in the compression index and coefficient of consolidation; the more significant change in compressibility and strength was seen when the acid solution became more acidic. Concerning the SEM test, the images demonstrate the formation of macro pores between soil particles due to soil contamination. As the HCl acid solution concentration increased, more pores were formed and irregularly distributed across the whole soil surface. HCl acid solution contamination of soil causes the soil characteristics to degrade generally. Construction on this soil would consequently need to take into account the environment. Preparing the soil before building on it is suggested by adding materials that can increase the acid resistance of the soil.</p></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influencing Factors and Spatial Spillover Effects of Pollution in the Yangtze River Delta–Based On a New Measurement Method","authors":"Chao Hua, Jianjun Miao, Junchao Feng, Xing Wang","doi":"10.1007/s11270-025-07761-1","DOIUrl":"10.1007/s11270-025-07761-1","url":null,"abstract":"<div><p>Compared with a single pollutant, the comprehensive pollution index can reflect the degree of urban pollution more comprehensively. This article introduces the tax weighting method to calculate the comprehensive pollution index in the Yangtze River Delta (YRD). Based on the panel data of cities in the YRD from 2005 to 2017, the Spatial Durbin Model is utilized to empirically analyze the influencing factors and spatial spillover effects of the comprehensive pollution index. The results show: (1) The pollution of the cities in the YRD is unevenly distributed, and the eastern coastal areas are facing more serious pollution emissions than the western areas. (2) Changes in the urban industrial structure show an N-shaped curve impact on local industrial pollution emissions. The spatial spillover effect of the changes in industrial structure on the pollution emissions of surrounding cities presents an inverted N-shaped curve characteristic. (3) Economic openness and GDP per capita have a positive impact on pollution emissions. Government control can reduce pollution emissions. The impact of human capital and foreign direct investment on pollution emissions shows uncertainty over time and space. (4) The research results under the tax weighting method which can be proved to be applicable are consistent with the existing conclusions.</p></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rafaela Ribeiro de Brito, Raíssa de Oliveira Ferreira, Wesley Rodrigues Soares, Abraão Tiago Batista Guimarães, Aline Sueli de Lima Rodrigues, Thiarlen Marinho da Luz, Alex Rodrigues Gomes, Letícia Paiva de Matos, Guilherme Malafaia
{"title":"Persistent Effects of Naturally Aged Polyethylene Terephthalate Microplastics on Physalaemus cuvieri Tadpoles: The Toxic Legacy Beyond Exposure","authors":"Rafaela Ribeiro de Brito, Raíssa de Oliveira Ferreira, Wesley Rodrigues Soares, Abraão Tiago Batista Guimarães, Aline Sueli de Lima Rodrigues, Thiarlen Marinho da Luz, Alex Rodrigues Gomes, Letícia Paiva de Matos, Guilherme Malafaia","doi":"10.1007/s11270-025-07758-w","DOIUrl":"10.1007/s11270-025-07758-w","url":null,"abstract":"<div><p>The increasing presence of microplastics (MPs) in aquatic ecosystems has raised concerns, mainly due to their adverse effects on aquatic organisms such as amphibians. This study evaluated the effects of exposure to naturally-aged polyethylene terephthalate (PET) microplastics on <i>Physalaemus cuvieri</i> tadpoles to investigate particle retention and toxicological impacts after cessation of exposure. The MPs were characterized through scanning electron microscopy and Raman spectroscopy analysis, and the weathering action was confirmed. Furthermore, our results indicated a significant increase in the mortality of tadpoles exposed to PET-MPs and partial retention of MPs after depuration. A higher Redox Balance Index (involving ROS levels and SOD and CAT activity) observed in the PET-MPs group at the end of the depuration period reveals a greater redox imbalance in these tadpoles. On the other hand, exposure to MPs induced neurochemical dysfunctions, such as reduced dopamine levels and increased AChE/BChE ratio, which were evident even after the end of exposure. Furthermore, the increase in total protein levels was observed throughout the experiment only in the control group. Finally, multivariate analyses (PCA and discriminant analysis) confirmed the occurrence of a phenomenon called the “legacy effect,” showing a clear separation between groups in the distinct phases of the experiment. In addition, multivariate linear regression analysis revealed a significant influence of MPs on the response of the animals only at the end of the exposure period, suggesting that the response observed at the end of depuration was cumulative, reflecting the damage induced during exposure. Thus, it is concluded that exposure to naturally-aged PET-MPs generates cumulative toxic effects in P. cuvieri, even after removing the pollutant, reinforcing the need for mitigation strategies to minimize the prolonged impacts of MPs on aquatic ecosystems.</p></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Di Zhang, Keqing Liu, Chengcheng Feng, Xianmin Wang, Ayat J. S. Al-Azab, Han Lu, Haiyan Ma, Ying Tang, Li Xu, Takeshi Ohama, Fantao Kong
{"title":"Physiological and transcriptomic responses of Chlorella vulgaris to novel antibacterial nanoparticles of ethyl cyanoacrylate polymer","authors":"Di Zhang, Keqing Liu, Chengcheng Feng, Xianmin Wang, Ayat J. S. Al-Azab, Han Lu, Haiyan Ma, Ying Tang, Li Xu, Takeshi Ohama, Fantao Kong","doi":"10.1039/d4en00861h","DOIUrl":"https://doi.org/10.1039/d4en00861h","url":null,"abstract":"The ethyl cyanoacrylate nanoparticles (ECA-NPs) have recently been reported as promising novel antibacterial NPs capable of inhibiting the growth of several Gram-positive and Gram-negative bacteria. However, the effects of ECA-NPs on microalgae, which are primary producers in aquatic ecosystems, remain unknown. In this study, we examined the effects of ECA-NPs on the microalga Chlorella vulgaris (Chlorella) at both cellular and molecular levels. A high concentration of ECA-NPs (100 μg/mL) exhibited strong growth inhibitory effects on Chlorella. In the ECA-NPs-treated cells, transmission electron microscope (TEM) observations showed the prominent internalization of ECA-NPs in the periplasmic space and vacuoles. Moreover, notable morphological changes such as a thinner cell wall, stacked thylakoid structure, and plasmolysis were observed. ECA-NPs exposed Chlorella secreted more extracellular polymeric substances (EPS) and accumulated more storage lipids (mainly triacylglycerol, TAG) compared to the control. However, the contents of total fatty acids and starch were decreased, and photosynthetic activity was reduced. In addition, the content of intracellular reactive oxygen species (ROS) and the activities of antioxidant enzymes in ECA-NPs-treated cells were significantly higher than in the control. Transcriptomic analysis revealed the downregulation of genes that are involved in drug binding/catabolic process, chemical stimulus detection, and cell wall component catabolic process (chitin catabolism), while genes involved in photosynthetic membrane and plastid thylakoid were upregulated. These results indicated that the effects of ECA-NPs exposure are not limited to specific metabolic pathways, but rather influence metabolic pathways across the entire cell. This study also provided new insights into the potential toxic effects associated with cyanoacrylate NPs in phytoplankton.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"24 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Son Hong Nguyen, Ngoc Toan Vu, Hoang Van Nguyen, Binh Nguyen, Trung Thien Luong
{"title":"Biologically synthesized Fe0-based nanoparticles and their application trends as catalysts in the treatment of chlorinated organic compounds: a review","authors":"Son Hong Nguyen, Ngoc Toan Vu, Hoang Van Nguyen, Binh Nguyen, Trung Thien Luong","doi":"10.1039/d4en00843j","DOIUrl":"https://doi.org/10.1039/d4en00843j","url":null,"abstract":"This review explores the advancements and trends in biologically synthesized Fe⁰-based nanoparticles (NPs) and their applications as catalysts in treating chlorinated organic compounds. The persistent nature and bioaccumulative characteristics of chlorinated organic compounds enable their accumulation in water, soil, and the food chain, leading to significant environmental and human health issues. The widespread presence of these toxic substances underscores the urgent need for effective treatment and remediation strategies. Biologically synthesized Fe⁰-based NPs are recognized for their considerable surface area, potent reduction properties, and environmental compatibility. These attributes render them a promising approach for the remediation of chlorinated compounds. This review categorizes synthesis methods into key groups: microorganisms, plant extracts, biological waste, and industrial-agricultural by-products. Recent studies highlight the promising applications of bio-NPs in environmental remediation, emphasizing their potential for sustainable and efficient treatment solutions. This analysis thoroughly examines current trends in the application and enhancement of nanoparticle activity, delineating various challenges and future prospects comprehensively. It offers well-defined research directions with high practical relevance, aiming to contribute to advancing knowledge and guiding future research endeavors in the field.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"57 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142990723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Expansion Microscopy Revealed Specific Impacts of Nano Zinc Oxide on Early Organ Development in Fish","authors":"Mengyu WANG, Wen-Xiong Wang","doi":"10.1039/d4en01071j","DOIUrl":"https://doi.org/10.1039/d4en01071j","url":null,"abstract":"Nanomaterials exhibit significant advantages in biomedical applications. However, their potential risks to organisms cannot be overlooked, particularly during early development. Traditional methods for assessing organ-specific toxicity are limited by their difficulty in exploring differences between materials at the nanoscale resolution. The novel expansion microscopy technique (ExM) provides an effective solution for high-resolution nanoscale imaging, allowing biological samples to be expanded approximately 4.5 times in three-dimensional space. ExM enables the visualization of proteins and nucleic acid targets in cells and tissues using conventional optical microscopy, achieving nanoscale imaging. The widespread application of nano zinc oxide (nZnO) in the biomedical field has raised concerns regarding toxicity. This study systematically assesses the toxicological changes and sources of nZnO and Zn²⁺ in the visual, skeletal muscle, and digestive systems. Our results indicated that appropriate concentrations of nZnO supported the normal early development in the visual and skeletal muscle systems, while potentially leading to excessive toxicity in the digestive system. Conversely, the concentrations of nZnO suitable for the development of the digestive system may be inadequate for the needs of the visual and skeletal muscle systems. This discrepancy may arise from differences in the solubility and bioaccessibility of nZnO in gastrointestinal fluids. Further RNA sequencing revealed differences in the sensitivity of various organs to nanomaterial exposure, highlighting the necessity of implementing comprehensive risk assessment strategies in toxicology. Overall, we visualized and quantified the subtle developmental toxicities of nZnO and Zn²⁺ across different organs for the first time. The application of expansion microscopy technique offered a novel perspective for evaluating the toxicity of nanomaterials.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"79 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142990726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Florian Lapp, Felix Brück, Jürgen Göske, Reiner Dohrmann, Tim Mansfeldt, Harald Weigand
{"title":"Fresh and Aged Chromite Ore Processing Residues (COPR): Weathering-Induced Alteration of Chemical Properties, Cr(VI) Mobility and Mineralogy At Open Dumpsites in Kanpur, India","authors":"Florian Lapp, Felix Brück, Jürgen Göske, Reiner Dohrmann, Tim Mansfeldt, Harald Weigand","doi":"10.1007/s11270-024-07726-w","DOIUrl":"10.1007/s11270-024-07726-w","url":null,"abstract":"<div><p>Chromite ore processing residue (COPR) is a hazardous waste retaining relic Cr(VI). Large amounts are generated during the high-lime production of leather tanning salts in the region of Kanpur, India. Here, COPR is often deposited on open and uncontrolled landfills, leading to severe groundwater contamination. This study aimed at elucidating how ageing under these ambient conditions alters COPR properties and Cr(VI) mobility. For this, aged COPR obtained from surface and subsurface horizons of a visibly weathered open dumpsite was systematically compared to fresh high-lime COPR collected at two tanning salt factories. Elemental composition of the samples was characterized using X-ray fluorescence analysis while Cr(VI) mobility was assessed photometrically in alkaline and aqueous batch extracts. Mineralogical composition of the COPR was studied using X-ray powder diffractometry, scanning electron microscopy and thermogravimetry–mass spectrometry. The fresh COPR were highly alkaline and contained characteristic Cr(VI) host phases like calcium aluminum chromate hydroxide (CAC) and katoite. These were absent in the aged samples due to their lower pH of ~ 9. The pH drop was likely caused by uptake of atmospheric CO<sub>2</sub>, which was corroborated by elevated carbon and calcite levels. This carbonation coincided with vertical translocation of Cr(VI) to the subsurface of the landfill, where leachate concentrations in excess of 1.6 g · L<sup>−1</sup> and chromatite (CaCrO<sub>4</sub>) precipitations were found. The results highlight the importance of carbonation as a key ageing process which will likely exacerbate Cr(VI) groundwater contamination at open COPR dumpsites.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11270-024-07726-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rodrigo Roucourt Cezário, Vinicius Marques Lopez, Felipe Datto-Liberato, Seth M. Bybee, Stanislav Gorb, Rhainer Guillermo-Ferreira
{"title":"Polarized vision in the eyes of the most effective predators: dragonflies and damselflies (Odonata)","authors":"Rodrigo Roucourt Cezário, Vinicius Marques Lopez, Felipe Datto-Liberato, Seth M. Bybee, Stanislav Gorb, Rhainer Guillermo-Ferreira","doi":"10.1007/s00114-025-01959-3","DOIUrl":"10.1007/s00114-025-01959-3","url":null,"abstract":"<div><p>Polarization is a property of light that describes the oscillation of the electric field vector. Polarized light can be detected by many invertebrate animals, and this visual channel is widely used in nature. Insects rely on light polarization for various purposes, such as water detection, improving contrast, breaking camouflage, navigation, and signaling during mating. Dragonflies and damselflies (Odonata) are highly visual insects with polarization sensitivity for water detection and likely also navigation. Thus, odonates can serve as ideal models for investigating the ecology and evolution of polarized light perception. We provide an overview of the current state of knowledge concerning polarized light sensitivity in these insects. Specifically, we review recent findings related to the ecological, morphological, and physiological causes that enable these insects to perceive polarized light and discuss the optical properties responsible for the reflection of polarized light by their bodies and wings. Finally, we identify gaps in the current research and suggest future directions that can help to further advance our knowledge of polarization sensitivity in odonates.</p></div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"112 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00114-025-01959-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Designing Safer Nanohybrids: Stability and Ecotoxicological Assessment of Graphene Oxide-Gold Nanoparticles Hybrids in Embryonic Zebrafish","authors":"Bashiru Ibrahim, Taiwo Hassan Akere, Pankti Dhumal, Eugenia Valsami-Jones, Swaroop Chakraborty","doi":"10.1039/d4en01173b","DOIUrl":"https://doi.org/10.1039/d4en01173b","url":null,"abstract":"Graphene oxide (GO) and graphene oxide-gold (GO-Au) nanohybrids offer promising applications in nanomedicine, biosensing, and environmental technology due to their unique properties. However, concerns regarding their environmental and biological safety remain largely unexplored. This study, using a Safe and Sustainable by Design (SSbD) approach, evaluates the cytotoxicity, oxidative stress, and dispersion stability of GO and GO-Au nanohybrids in zebrafish ZF4 cells. GO was synthesised using a modified Hummer’s method and GO-Au nanohybrids were prepared by incorporating gold nanoparticles (AuNPs) into the GO matrix. Physicochemical characterisation revealed enhanced dispersion stability of GO-Au nanohybrids, retaining over 98% of their initial absorbance in ultrapure water (UPW) and over 95% in DMEM/F12 after 48 hours. In contrast, GO displayed higher levels of sedimentation. Toxicity assessments indicated a dose- and time-dependent decrease in cell viability. After 72 hours, ZF4 cell viability was reduced to 39.5% at 150 µg/mL of GO, whereas GO-Au treatment at the same concentration exhibited a less severe reduction (54.5% viability). Reactive oxygen species (ROS) generation was significantly higher in GO-treated cells compared to GO-Au, with GO generating approximately 2x more ROS at concentrations of 50 µg/mL and 100 µg/mL. Apoptosis and necrosis rates were also significantly elevated in GO-treated cells, with necrosis reaching 53.1% at 100 µg/mL, compared to 14.6% in GO-Au-treated cells. The findings demonstrate that the incorporation of AuNPs reduce cytotoxicity and oxidative stress by enhancing the colloidal stability of GO-Au nanohybrids. This study provides critical baseline data on the interaction of GO-based nanomaterials (NMs) with biological systems and highlights the importance of NMs modification for safer, more sustainable applications.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"33 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142992328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Ansari, Afsana Munni, Dianne Carrillo, Matthew Pedersen, Rafiqul Islam, Francois Perreault
{"title":"Coating of complex metallic surfaces with passivated silver nanoparticles for long-term biofilm control","authors":"Ali Ansari, Afsana Munni, Dianne Carrillo, Matthew Pedersen, Rafiqul Islam, Francois Perreault","doi":"10.1039/d4en00797b","DOIUrl":"https://doi.org/10.1039/d4en00797b","url":null,"abstract":"Deep space missions will bring new challenges, beyond our experience so far with International Space Station, to life support systems including water supply. The complexity of these missions might leave spacecrafts and facilities uncrewed for several months. In this situation, biofilm growth can deteriorate the quality of stored water and cause water supply system failure during reinitiation, threating the mission success. Antimicrobial coatings have been used for biofilm mitigation in various conditions. A successful coating to control biofilm formation in deep space mission, among other things, must have long lifetime considering the duration of such missions. In this study, a solution was provided to the biggest drawback of silver nanoparticles as antimicrobial coating; short lifetime. Passivating with sulfide was tested to control silver ion release from silver nanoparticles, hence, prolonging antimicrobial activity. Stainless steel bellow pieces, as the most prone parts to biofilm growth, was chosen as the substrate. The pieces were coated with silver and passivated silver with different passivation degree to find the optimum condition. The substrates were exposed to Pseudomonas aeruginosa in M9 medium for 12 months for biofilm formation. The bacteria count on the bellow pieces as a representative of biofilm as well as bacteria count and silver ion concentration in M9 medium were measured at 1.5, 3, 6, and 12-month timepoints. Passivation slowed down silver ion release rate from silver nanoparticles, however, biofilm mitigation at the end of the experiment for one passivated coating was the same as silver coating, which means the passivated coating can last longer by releasing less antimicrobial agent, silver ions. Besides performance in biofilm mitigation, we demonstrated that the bellows can be coated homogeneously in a continuous reactor and passivation can enhance the stability of the coating to mechanical stress during expansion/retraction of the bellow, paving the way for application of passivated silver coating for space missions.","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"102 1","pages":""},"PeriodicalIF":8.131,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}