生命科学最新文献

{"title":"ONAC005 enhances salt stress tolerance by promoting suberin deposition in root endodermis","authors":"Yeonjoon Kim,&nbsp;Boyeong Kim,&nbsp;Jinku Kang,&nbsp;Sang-Il Bae,&nbsp;Hyeryung Yoon,&nbsp;Hee-Ji Shin,&nbsp;Ji-Young Lee,&nbsp;Nam-Chon Paek,&nbsp;Kiyoon Kang","doi":"10.1111/tpj.70469","DOIUrl":"https://doi.org/10.1111/tpj.70469","url":null,"abstract":"<p>Salt stress impairs photosynthetic efficiency and consequently reduces the growth, development, and grain yield of crop plants. The formation of hydrophobic barriers in the root endodermis, including the suberin lamellae and Casparian strips, is a key adaptive strategy for salt stress tolerance. In this study, we identified the role of the rice NAC transcription factor, ONAC005, in salt stress tolerance. <i>ONAC005</i> expression was induced by NaCl and abscisic acid (ABA). Expression analysis using the β-glucuronidase reporter gene driven by the <i>ONAC005</i> promoter revealed that <i>ONAC005</i> is predominantly expressed in the stele and endodermis of rice roots. The null mutation of <i>ONAC005</i> increased sodium ion levels in the shoots and roots, indicating susceptibility to salt stress, whereas <i>ONAC005</i> overexpression enhanced tolerance to salt stress by reducing sodium ion accumulation. Yeast one-hybrid, chromatin immunoprecipitation, and dual-luciferase assays demonstrated that ONAC005 upregulates the expression of <i>trehalose-6-phosphate synthase 8</i> (<i>OsTPS8</i>) by directly binding to its promoter region, leading to increased trehalose accumulation. <i>ONAC005</i> enhances the formation of the root hydrophobic barrier by upregulating <i>OsTPS8</i> expression under salt stress. Furthermore, considering the altered expression of ABA signaling and responsive genes, ONAC005 regulates the expression of genes in specific stress-responsive pathways that are independent of <i>OsTPS8</i>-mediated signaling. These results indicate that <i>ONAC005</i> positively regulates hydrophobic barrier formation in the roots, thereby enhancing salt stress tolerance in rice.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.","authors":"Muthusamy Ramakrishnan, Rashmi Kaul, Anket Sharma, Zishan Ahmad, Venkatesan Vijayakanth, Krishnamurthi Keerthana, Zhipeng Gao, Mingbing Zhou, Qiang Wei","doi":"10.1111/pce.70176","DOIUrl":"https://doi.org/10.1111/pce.70176","url":null,"abstract":"<p><p>CRISPR ribonucleoprotein (RNP)-mediated genome editing offers a transgene-free platform for precise genetic modification in diverse herbaceous and tree species, including rice, wheat, apple, poplar, oil palm, rubber tree and grapevine. However, its application in woody plants faces distinct challenges, notably inefficient delivery and regeneration difficulties, particularly in species such as bamboo. While some of these issues also occur in herbaceous plants, they are often significantly more complex in woody species due to factors such as intricate cell wall architecture, widespread recalcitrant genotypes and inherent limitations of current delivery platforms. This review presents the first in-depth, critical re-evaluation of recent advancements in RNP-mediated editing in woody plants, highlighting these obstacles that warrant focused attention. Unlike plasmid-based CRISPR systems, RNP editing utilises Cas9/Cas12a protein-guide RNA complexes without integrating foreign DNA. This enables a DNA-free editing strategy that simplifies regulatory approval and minimises off-target effects due to the transient presence and rapid degradation of RNPs within plant cells. While PEG-mediated protoplast transfection and particle bombardment remain the primary reported methods for RNP delivery in trees, we evaluate promising alternative strategies such as lipofection, electroporation, cell-penetrating peptides and nanoparticle-based systems for targeted RNP delivery. Despite their promise, these advanced methods remain largely untested in woody species. Finally, we outline future research directions, including the development of tree-specific RNP delivery systems and regeneration protocols to enhance efficiency and minimise cytotoxicity. These innovations are essential for unlocking the full potential of RNP-mediated genome editing in long-lived tree species. This review provides a focused and timely roadmap for expanding the application of RNP technology across diverse woody plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progestogens and androgens influence root morphology of angiosperms in a brassinosteroid-independent manner","authors":"Karl Ludwig Körber,&nbsp;Sudip Paul,&nbsp;Jana Oklestkova,&nbsp;Emanuel Barth,&nbsp;Felix Feistel,&nbsp;Henk Oppermann,&nbsp;Ceren Oktay,&nbsp;Maja Dorfner,&nbsp;Miroslav Strnad,&nbsp;Jennifer Munkert,&nbsp;Alexandra C. U. Furch,&nbsp;Jan Klein","doi":"10.1111/tpj.70459","DOIUrl":"https://doi.org/10.1111/tpj.70459","url":null,"abstract":"<p>Progestogens and androgens are steroids found in a wide range of plants, but little is known about their physiological functions. In this study, we sowed seeds of angiosperms on progestogen- and androgen-containing medium and analysed their morphological effects. We further investigated the effects of progesterone and testosterone on brassinosteroid profiles and gene expression in <i>A. thaliana</i>. Additionally, we examined the effects of progesterone and testosterone on <i>A. thaliana</i> plants overexpressing the steroid 5α-reductase DET2. We found that progestogens and androgens have strong negative effects on root length, especially in <i>Brassicaceae</i> species. In addition, these steroids led to uncoordinated cell growth and increased lateral root formation. We failed to detect an effect on endogenous brassinosteroid levels and gene expression of brassinosteroid-regulated genes. The overexpression of DET2 led to increased root growth, but the effects of progesterone and testosterone were not reduced. We conclude that progestogens and androgens act in a brassinosteroid-independent manner. This suggests that progestogens and androgens could represent a potential new class of plant steroid signalling molecules.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Testing the validity of the Montgomery–Koyama–Smith equation and the power law equation using 3231 tepals of a Magnolia species","authors":"Linli Deng,&nbsp;Jinfeng Wang,&nbsp;Li Zhang,&nbsp;Dirk Hölscher,&nbsp;Peijian Shi","doi":"10.1007/s00468-025-02660-8","DOIUrl":"10.1007/s00468-025-02660-8","url":null,"abstract":"","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00468-025-02660-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repeated Soil Droughts Induce Differential Responses Across Leaflets of a Compound Leaf.","authors":"Tomasz Hura, Katarzyna Hura, Agnieszka Ostrowska, Karolina Urban, Kinga Dziurka, Bożena Pawłowska","doi":"10.1111/pce.70175","DOIUrl":"https://doi.org/10.1111/pce.70175","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRINKLY4: Multifaceted Roles Beyond Epidermal Cell Differentiation in Plant Development?","authors":"Xin-Ge Zhao, Ying Sun, Cui-Xia Pu","doi":"10.1111/pce.70159","DOIUrl":"https://doi.org/10.1111/pce.70159","url":null,"abstract":"<p><p>Receptor-like kinases (RLKs) play essential roles in plant growth and development. CRINKLY4 (CR4), one of the first reported RLKs in plants, is a well-known regulator of epidermal cell differentiation during leaf and seed development in maize. Within the last four decades, the functional landscape of CR4 has emerged across diverse developmental contexts and species, including dicots (e.g., Arabidopsis Thaliana), monocots (e.g., Oryza sativa and Zea mays), and even moss (e.g., Physcomitrella patens). CR4 is currently considered to be a multifaced regulator of plant development beyond epidermal cell differentiation. In this review, we highlight the versatile roles of CR4 based on its expression and dynamic subcellular localisation patterns, we discuss the importance of its two extracellular domains and kinase activity for its functionality, and we summarise the CR4-mediated signalling pathways underlying columella stem cell differentiation, epidermal cell differentiation, and vascular bundle formation. Finally, we raise several open questions for future research.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Front Cover Image","authors":"Yong Long,&nbsp;Yansheng Li,&nbsp;Jing Zhang,&nbsp;Jiaxuan Liu,&nbsp;Qingqing Han,&nbsp;Yingxue Cao,&nbsp;Yijia Jiang,&nbsp;Changkai Liu,&nbsp;Yanfeng Hu,&nbsp;Guanghua Wang,&nbsp;Xueyan Zhang,&nbsp;Jian Jin,&nbsp;Mikhail Semenov,&nbsp;Guanran Han,&nbsp;Xiaobing Liu,&nbsp;Zhenhua Yu","doi":"10.1111/pce.70170","DOIUrl":"https://doi.org/10.1111/pce.70170","url":null,"abstract":"<p>The cover image is based on the article <i>Unconventional Nitrogen Fixation and Adaptive Genomics of a New Neorhizobium glycines sp. nov., A Promising Soybean Symbiont</i> by Yong Long et al., https://doi.org/10.1111/pce.70046.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 10","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pce.70170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 'Green Code' in Fruit Cultivation: Research Progress and Prospects of Rootstock Control Tree Dwarfing.","authors":"Wei-Feng Ma, Zong-Huan Ma, Ying-Jun Hou, Shi-Xiong Lu, Guo-Ping Liang, Juan Mao, Bai-Hong Chen","doi":"10.1111/pce.70151","DOIUrl":"https://doi.org/10.1111/pce.70151","url":null,"abstract":"<p><p>This review discusses the research progress of regulating tree dwarfing in fruit tree rootstocks, including its definition, manifestation, mechanism and application of different rootstocks. Studies indicate that dwarfing rootstocks reduce vegetative growth while promoting reproductive growth. Compared with vigorous rootstocks, the contents of indole-3-acetic acid, cytokinin, and gibberellin in leaves is lower, while the content of abscisic acid is higher. In addition, root structure, water and nutrient absorption capacity, hormone level and gene expression of rootstocks also affect the dwarfing effect. It was also found that dwarfing rootstocks had strong environmental adaptability, which could improve the stress resistance and fruit quality of fruit trees.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsPIL1 Differentially Modulates Rice Blast Resistance Through Integrating Light or Darkness During Magnaporthe oryzae Infection.","authors":"Tianqi Zhao, Ping Tang, Rubin Zuo, Shumin Yang, Juye Tong, Runxue Xie, Liqing Yang, Jing Yang","doi":"10.1111/pce.70168","DOIUrl":"https://doi.org/10.1111/pce.70168","url":null,"abstract":"<p><p>Light and darkness are critical environmental factors that regulate plant immune responses. OsPIL1, a phytochrome-interacting factor-like protein, has been implicated in rice immunity against Magnaporthe oryzae, although its underlying mechanism remains unclear. This study aimed to dissect how OsPIL1 integrates light or darkness to modulate rice immunity. OsPIL1-overexpressing (OsPIL1 OE), Ospil1 knockout (ΔOspil1) and wild-type (WT) rice plants were pre-treated with light or darkness before inoculation. Blast disease symptoms, transcriptional changes in defence-related genes, and phytohormone levels were analysed to elucidate OsPIL1-associated defence responses. Transcriptomic (RNA-seq), proteomic (IP-MS) and protein interaction (Co-IP) analyses were employed to identify OsPIL1-regulated genes and interacting proteins. We found that light and darkness had contrasting effects on OsPIL1 OE plants. Darkness pre-treatment enhanced resistance in OsPIL1 OE plants, whereas light pre-treatment severely compromised it, exacerbating disease symptoms. Crucially, the ΔOspil1 ko plants exhibited increased susceptibility compared to WT, a phenotype that was significantly more pronounced under light, confirming that OsPIL1 functions as a negative regulator of immunity in a light-dependent manner. These phenotypes were correlated with corresponding changes in fungal colonisation, defence gene transcription and phytohormone profiles. RNA-seq analysis revealed differential expression of genes related to signalling pathways (kinases, phosphatases, transcription factors/repressors and ubiquitin-related proteins) under light and darkness in OsPIL1 OE plants. IP‒MS identified the cell wall invertase OsINV3 as a candidate interacting partner of OsPIL1, an interaction was confirmed via Co-IP assays. The results of functional assays suggest that this interaction contributes to resistance, potentially by modulating sugar signalling. Collectively, these findings demonstrate that OsPIL1 is a key signalling hub that negatively regulates rice immunity in the presence of light, with partial resistance retained in darkness through OsINV3 interaction. This study reveals a crucial light-dependent immune regulatory mechanism and offers potential targets for improving disease resistance in rice through molecular breeding or agronomic intervention.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An oomycete effector targets host calmodulin to suppress plant immunity","authors":"Peng Li,&nbsp;Lizhu Xie,&nbsp;Wen Li,&nbsp;Gangqiang Zhou,&nbsp;Junjian Situ,&nbsp;Zijing Zhang,&nbsp;Minhui Li,&nbsp;Pinggen Xi,&nbsp;Zide Jiang,&nbsp;Guanghui Kong","doi":"10.1111/tpj.70457","DOIUrl":"https://doi.org/10.1111/tpj.70457","url":null,"abstract":"<div>\u0000 \u0000 <p>Tropical and subtropical fruit trees face serious threats of oomycete-caused plant diseases. However, the molecular mechanism by which oomycete pathogens suppress the immunity of these fruit trees remains largely unclear. Effectors play a crucial role in the pathogenesis of plant pathogenic oomycetes. Here, we found that a conserved RXLR-type effector protein PlAvh222 from the pathogen <i>Peronophythora litchii</i> is required for its full virulence on litchi. Expression of PlAvh222 in <i>Nicotiana benthamiana</i> leaves suppressed INF1-induced immune responses and promoted <i>Phytophthora capsici</i> infection. Further research demonstrated that PlAvh222 interacted with litchi calmodulins (LcCaMs) <i>in vivo</i> and <i>in vitro</i>. Silencing of <i>NbCaM1/2/3/4</i> attenuated the ability of PlAvh222 to enhance <i>N</i>. <i>benthamiana</i> susceptibility. The C-terminal CaM-binding region of PlAvh222 is required for targeting LcCaM and to suppress <i>N</i>. <i>benthamiana</i> immune responses, including programmed cell death (PCD) and reactive oxygen species (ROS) burst. In addition, the interaction between PlAvh222 and LcCaM1/2/3 increases the accumulation of LcCaM1/2/3 and reduces levels of cytosolic Ca²⁺ ([Ca²⁺]_cyt). Blocking [Ca²⁺]_cyt influx leads to compromised PCD in <i>N</i>. <i>benthamiana</i>. Our results reveal that the oomycete effector promotes pathogen infection through suppressing [Ca²⁺]_cyt-induced plant immunity.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}