Qiyun Kang , Ziting Zhu , Zhongrui Liu , Fei Li , Yan He , Yaru Yang , Xutao Wang , Shuisheng Lei , Zishu Yuan , Xiaoqin Zhu
{"title":"一种新型硫化氢供体通过激活 ATP 敏感性钾通道减轻神经炎症和癫痫发作","authors":"Qiyun Kang , Ziting Zhu , Zhongrui Liu , Fei Li , Yan He , Yaru Yang , Xutao Wang , Shuisheng Lei , Zishu Yuan , Xiaoqin Zhu","doi":"10.1016/j.neures.2023.07.004","DOIUrl":null,"url":null,"abstract":"<div><p>Epilepsy is a common neurological disorder worldwide. Hydrogen sulfide (H<sub>2</sub>S) has been found to have anti-seizure effects. However, its mechanism remains to be explored. In the present study, we showed that a novel H<sub>2</sub>S donor attenuated neuroinflammation by up-regulating ATP-sensitive potassium channel (K<sub>ATP</sub>) expression to reduce seizures. The novel H<sub>2</sub>S donor significantly reduced the expression of TNF-α and increased the expression of IL-10 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. The modulatory effects of the H<sub>2</sub>S donor on inflammatory cytokines were prevented by glibenclamide, a common K<sub>ATP</sub> channels blocker. The H<sub>2</sub>S donor promoted the expression of K<sub>ATP</sub> channel subunits SUR2 and Kir6.1 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. In addition, the H<sub>2</sub>S donor reduced the electroencephalography amplitude of hippocampal epileptic waves and reduced seizures in pilocarpine-induced epileptic mice, which were also attenuated by glibenclamide. These results indicated that the novel H<sub>2</sub>S donor reduced seizures and regulated microglial inflammatory cytokines by activating K<sub>ATP</sub> channels, which may provide a prospective therapeutic strategy for the anti-seizure effects of H<sub>2</sub>S donor.</p></div>","PeriodicalId":19146,"journal":{"name":"Neuroscience Research","volume":"199 ","pages":"Pages 21-29"},"PeriodicalIF":2.4000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168010223001396/pdfft?md5=24b63cd4d3dd97e0a616c261cc5db443&pid=1-s2.0-S0168010223001396-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A novel hydrogen sulfide donor reduces neuroinflammation and seizures by activating ATP-sensitive potassium channels\",\"authors\":\"Qiyun Kang , Ziting Zhu , Zhongrui Liu , Fei Li , Yan He , Yaru Yang , Xutao Wang , Shuisheng Lei , Zishu Yuan , Xiaoqin Zhu\",\"doi\":\"10.1016/j.neures.2023.07.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Epilepsy is a common neurological disorder worldwide. Hydrogen sulfide (H<sub>2</sub>S) has been found to have anti-seizure effects. However, its mechanism remains to be explored. In the present study, we showed that a novel H<sub>2</sub>S donor attenuated neuroinflammation by up-regulating ATP-sensitive potassium channel (K<sub>ATP</sub>) expression to reduce seizures. The novel H<sub>2</sub>S donor significantly reduced the expression of TNF-α and increased the expression of IL-10 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. The modulatory effects of the H<sub>2</sub>S donor on inflammatory cytokines were prevented by glibenclamide, a common K<sub>ATP</sub> channels blocker. The H<sub>2</sub>S donor promoted the expression of K<sub>ATP</sub> channel subunits SUR2 and Kir6.1 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. In addition, the H<sub>2</sub>S donor reduced the electroencephalography amplitude of hippocampal epileptic waves and reduced seizures in pilocarpine-induced epileptic mice, which were also attenuated by glibenclamide. These results indicated that the novel H<sub>2</sub>S donor reduced seizures and regulated microglial inflammatory cytokines by activating K<sub>ATP</sub> channels, which may provide a prospective therapeutic strategy for the anti-seizure effects of H<sub>2</sub>S donor.</p></div>\",\"PeriodicalId\":19146,\"journal\":{\"name\":\"Neuroscience Research\",\"volume\":\"199 \",\"pages\":\"Pages 21-29\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0168010223001396/pdfft?md5=24b63cd4d3dd97e0a616c261cc5db443&pid=1-s2.0-S0168010223001396-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neuroscience Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0168010223001396\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroscience Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168010223001396","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
A novel hydrogen sulfide donor reduces neuroinflammation and seizures by activating ATP-sensitive potassium channels
Epilepsy is a common neurological disorder worldwide. Hydrogen sulfide (H2S) has been found to have anti-seizure effects. However, its mechanism remains to be explored. In the present study, we showed that a novel H2S donor attenuated neuroinflammation by up-regulating ATP-sensitive potassium channel (KATP) expression to reduce seizures. The novel H2S donor significantly reduced the expression of TNF-α and increased the expression of IL-10 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. The modulatory effects of the H2S donor on inflammatory cytokines were prevented by glibenclamide, a common KATP channels blocker. The H2S donor promoted the expression of KATP channel subunits SUR2 and Kir6.1 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. In addition, the H2S donor reduced the electroencephalography amplitude of hippocampal epileptic waves and reduced seizures in pilocarpine-induced epileptic mice, which were also attenuated by glibenclamide. These results indicated that the novel H2S donor reduced seizures and regulated microglial inflammatory cytokines by activating KATP channels, which may provide a prospective therapeutic strategy for the anti-seizure effects of H2S donor.
期刊介绍:
The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience
Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.