一种新型硫化氢供体通过激活 ATP 敏感性钾通道减轻神经炎症和癫痫发作

IF 2.4 4区 医学 Q3 NEUROSCIENCES
Qiyun Kang , Ziting Zhu , Zhongrui Liu , Fei Li , Yan He , Yaru Yang , Xutao Wang , Shuisheng Lei , Zishu Yuan , Xiaoqin Zhu
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引用次数: 0

摘要

癫痫是全球常见的神经系统疾病。研究发现,硫化氢(H2S)具有抗癫痫作用。然而,其机制仍有待探索。在本研究中,我们发现一种新型 H2S 供体通过上调 ATP 敏感钾通道(KATP)的表达来减轻神经炎症,从而减少癫痫发作。新型 H2S 供体能显著降低经 LPS 处理的 BV2 细胞和皮质激素诱导的癫痫小鼠海马中 TNF-α 的表达,并增加 IL-10 的表达。格列本脲是一种常见的 KATP 通道阻断剂,它能阻止 H2S 供体对炎症细胞因子的调节作用。H2S 供体促进了经 LPS 处理的 BV2 细胞和皮质类固醇诱导的癫痫小鼠海马中 KATP 通道亚基 SUR2 和 Kir6.1 的表达。此外,H2S 供体还降低了海马癫痫波的脑电图振幅,减少了皮质类药物诱导的癫痫小鼠的癫痫发作,而格列本脲(glibenclamide)也可减轻癫痫发作。这些结果表明,新型 H2S 供体可通过激活 KATP 通道减少癫痫发作并调节小胶质细胞炎症细胞因子,这可能为 H2S 供体的抗癫痫作用提供了一种前瞻性治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel hydrogen sulfide donor reduces neuroinflammation and seizures by activating ATP-sensitive potassium channels

Epilepsy is a common neurological disorder worldwide. Hydrogen sulfide (H2S) has been found to have anti-seizure effects. However, its mechanism remains to be explored. In the present study, we showed that a novel H2S donor attenuated neuroinflammation by up-regulating ATP-sensitive potassium channel (KATP) expression to reduce seizures. The novel H2S donor significantly reduced the expression of TNF-α and increased the expression of IL-10 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. The modulatory effects of the H2S donor on inflammatory cytokines were prevented by glibenclamide, a common KATP channels blocker. The H2S donor promoted the expression of KATP channel subunits SUR2 and Kir6.1 in LPS-treated BV2 cells and the hippocampus of pilocarpine-induced epileptic mice. In addition, the H2S donor reduced the electroencephalography amplitude of hippocampal epileptic waves and reduced seizures in pilocarpine-induced epileptic mice, which were also attenuated by glibenclamide. These results indicated that the novel H2S donor reduced seizures and regulated microglial inflammatory cytokines by activating KATP channels, which may provide a prospective therapeutic strategy for the anti-seizure effects of H2S donor.

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来源期刊
Neuroscience Research
Neuroscience Research 医学-神经科学
CiteScore
5.60
自引率
3.40%
发文量
136
审稿时长
28 days
期刊介绍: The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.
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