阿魏酸环蒿烯基对γ辐照水稻(发芽水稻)α-葡萄糖苷酶和α-淀粉酶的抑制作用及其浓度升高。

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY
Sompong Sansenya, Apirak Payaka, Preecha Mansalai
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引用次数: 0

摘要

阿魏酸环artenyl是γ-谷米醇的衍生物,具有多种生物活性,包括治疗糖尿病。研究了在生理盐水条件下伽马辐照对萌发水稻阿魏酸环artenyl积累的促进作用。此外,通过体外和硅技术研究了阿魏酸环artenyl对碳水化合物水解酶(α-葡萄糖苷酶和α-淀粉酶)的抑制潜力。结果表明,经γ辐照后,在盐水条件下萌发的水稻中阿魏酸环artenyl含量增加。在100 Gy和40 mM盐浓度下,刺激萌发水稻中阿魏酸环artenyl的最高浓度为852.20±20.59 μg/g。阿魏酸环artenyl对α-葡萄糖苷酶的抑制电位(31.31±1.43%)高于α-淀粉酶(12.72±1.11%)。阿魏酸环artenyl对α-葡萄糖苷酶的抑制模式为混合型抑制。荧光研究证实阿魏酸环artenyl与α-葡萄糖苷酶活性位点相互作用。对接研究表明,阿魏酸环artenyl与α-葡萄糖苷酶的7个氨基酸结合,结合能为-8.8 kcal/mol,结合电位高于α-淀粉酶(-8.2 kcal/mol)。结果表明,在生理盐水条件下,伽马辐照技术可以刺激γ-谷米醇,尤其是阿魏酸环蒿烯。此外,阿魏酸环artenyl证明了其作为糖尿病治疗中血糖管理的候选化合物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Inhibitory Efficacy of Cycloartenyl Ferulate against α-Glucosidase and α-Amylase and Its Increased Concentration in Gamma-Irradiated Rice (Germinated Rice).

Inhibitory Efficacy of Cycloartenyl Ferulate against α-Glucosidase and α-Amylase and Its Increased Concentration in Gamma-Irradiated Rice (Germinated Rice).

Inhibitory Efficacy of Cycloartenyl Ferulate against α-Glucosidase and α-Amylase and Its Increased Concentration in Gamma-Irradiated Rice (Germinated Rice).

Inhibitory Efficacy of Cycloartenyl Ferulate against α-Glucosidase and α-Amylase and Its Increased Concentration in Gamma-Irradiated Rice (Germinated Rice).

Cycloartenyl ferulate is a derivative of γ-oryzanol with varied biological activity, including diabetes mellitus treatment. This research focused on improving the cycloartenyl ferulate accumulation in germinated rice by gamma irradiation under saline conditions. Moreover, the inhibitory potential of cycloartenyl ferulate against carbohydrate hydrolysis enzymes (α-glucosidase and α-amylase) was investigated through in vitro and in silico techniques. The results revealed that cycloartenyl ferulate increased in germinated rice under saline conditions upon gamma irradiation. A suitable condition for stimulating the highest cycloartenyl ferulate concentration (852.20±20.59 μg/g) in germinated rice was obtained from the gamma dose at 100 Gy and under 40 mM salt concentration. The inhibitory potential of cycloartenyl ferulate against α-glucosidase (31.31±1.43%) was higher than against α-amylase (12.72±1.11%). The inhibition mode of cycloartenyl ferulate against α-glucosidase was demonstrated as a mixed-type inhibition. A fluorescence study confirmed that the cycloartenyl ferulate interacted with the α-glucosidase's active site. A docking study revealed that cycloartenyl ferulate bound to seven amino acids of α-glucosidase with a binding energy of -8.8 kcal/mol and a higher binding potential than α-amylase (-8.2 kcal/mol). The results suggested that the gamma irradiation technique under saline conditions is suitable for stimulating γ-oryzanol, especially cycloartenyl ferulate. Furthermore, cycloartenyl ferulate demonstrated its potential as a candidate compound for blood glucose management in diabetes mellitus treatment.

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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
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