Mahanthesh Vasudha, Chakra S Prashantkumar, Mallika Bellurkar, Vishwas Kaveeshwar, Devaraja Gayathri
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LAB were screened using 5-bromo-4-chloro-3-indole-D-galactopyranoside (X-Gal) and <i>O</i>-nitrophenyl-β-D-galactopyranoside (ONPG) as substrate, and exhibited β-galactosidase activity ranging from 728.25 to 1,203.32 Miller units. The most promising isolates were selected for 16S rRNA gene sequence analysis and identified as <i>Lactiplantibacillus plantarum</i>, <i>Limosilactobacillus fermentum</i>, <i>Lactiplantibacillus pentosus</i> and <i>Lactiplantibacillus</i> sp. Furthermore, these isolates were evaluated by <i>in vitro, viz.</i>, survival in gastrointestinal tract, antibiotic susceptibility, antimicrobial activity, cell surface characteristics, and haemolytic activity. 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引用次数: 4
摘要
近年来,益生菌因其调节肠道微生物群和对胃肠道健康的益处而引起了人们的极大兴趣。许多发酵食品含有乳酸菌(LAB),被认为是GRAS和益生菌。本研究旨在从印度卡纳塔克邦(Karnataka)偏远地区的自制发酵乳样品中提取本地乳酸菌,以分离出最有效和最适应当地环境条件的细菌,然后采用逐步评估益生菌特性和β-半乳糖苷酶生产能力的方法对其进行评估。以5-溴-4-氯-3-吲哚- d -半乳糖苷(X-Gal)和o-硝基苯-β- d -半乳糖苷(ONPG)为底物筛选LAB,其β-半乳糖苷酶活性范围为728.25 ~ 1203.32 Miller单位。选择最有希望的分离株进行16S rRNA基因序列分析,鉴定为植物乳杆菌(Lactiplantibacillus plantarum)、发酵乳杆菌(Limosilactobacillus fermentum)、肉芽孢杆菌(Lactiplantibacillus pentosus)和乳杆菌(Lactiplantibacillus sp.)。并对这些分离株进行了体外胃肠道存活、抗生素敏感性、抗菌活性、细胞表面特征和溶血活性等评价。所有8个分离株都表现出很强的粘附性,并阻止病原体渗透到HT-29细胞中,这表明细菌有可能扩大乳糖不耐症乳制品的工业生产规模。
Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization.
Probiotics have attained significant interest in recent years as a result of their gut microbiome modulation and gastrointestinal health benefits. Numerous fermented foods contain lactic acid bacteria (LAB) which are considered as GRAS and probiotic bacteria. The present study aimed to investigate indigenous LAB from homemade fermented milk samples collected in remote areas of Karnataka (India), in order to isolate the most potent and well-adapted to local environmental conditions bacteria, which were then evaluated using a step-by-step approach focused on the evaluation of probiotic traits and β-galactosidase-producing ability. LAB were screened using 5-bromo-4-chloro-3-indole-D-galactopyranoside (X-Gal) and O-nitrophenyl-β-D-galactopyranoside (ONPG) as substrate, and exhibited β-galactosidase activity ranging from 728.25 to 1,203.32 Miller units. The most promising isolates were selected for 16S rRNA gene sequence analysis and identified as Lactiplantibacillus plantarum, Limosilactobacillus fermentum, Lactiplantibacillus pentosus and Lactiplantibacillus sp. Furthermore, these isolates were evaluated by in vitro, viz., survival in gastrointestinal tract, antibiotic susceptibility, antimicrobial activity, cell surface characteristics, and haemolytic activity. All eight isolates demonstrated strong adherence and prevented pathogen penetration into HT-29 cells, indicating potential of the bacteria to scale up industrial level production of milk products for lactose intolerants.
期刊介绍:
Biomedical Reports is a monthly, peer-reviewed journal, dedicated to publishing research across all fields of biology and medicine, including pharmacology, pathology, gene therapy, genetics, microbiology, neurosciences, infectious diseases, molecular cardiology and molecular surgery. The journal provides a home for original research, case reports and review articles.