印度树(Plukenetia volubilis L.)油脂通过胰岛素信号通路改善2型糖尿病大鼠肝脏胰岛素敏感性和葡萄糖代谢

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY
Worarat Rojanaverawong, Navinee Wongmanee, Wanthanee Hanchang
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引用次数: 1

摘要

本研究旨在探讨核桃油(sacha inchi oil, SI)在2型糖尿病大鼠模型中通过抑制氧化应激和炎症来缓解肝脏胰岛素抵抗和改善葡萄糖代谢的作用。该模型是通过给大鼠高脂肪饮食和链脲佐菌素诱导糖尿病建立的。糖尿病大鼠每天口服0.5、1、2 mL/kg体重的SI或30 mg/kg体重的吡格列酮,连续5周。血液和肝组织用于胰岛素敏感性、碳水化合物代谢、氧化应激和炎症状态评估。SI治疗可降低糖尿病大鼠的高血糖和胰岛素抵抗指数,改善肝脏组织病理学改变,并呈剂量依赖性,这与降低血清中肝酶、丙氨酸转氨酶和天冬氨酸转氨酶水平有关。SI通过抑制丙二醛,提高抗氧化超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性,显著降低糖尿病大鼠肝脏氧化状态。此外,SI显著降低了糖尿病大鼠肝脏中促炎细胞因子水平,包括肿瘤坏死因子-α和白细胞介素-6。此外,SI处理增强了糖尿病大鼠肝脏胰岛素敏感性,表现为胰岛素受体底物-1和p-Akt蛋白表达增加,磷酸烯醇丙酮酸羧激酶-1和葡萄糖-6-磷酸酶蛋白表达降低,肝糖原含量增加。总的来说,这些发现表明,SI在2型糖尿病大鼠中具有潜在的肝脏胰岛素增敏作用,并改善葡萄糖代谢,至少部分是通过增强胰岛素信号传导、抗氧化防御和抑制炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sacha Inchi (<i>Plukenetia volubilis</i> L.) Oil Improves Hepatic Insulin Sensitivity and Glucose Metabolism through Insulin Signaling Pathway in a Rat Model of Type 2 Diabetes.

Sacha Inchi (<i>Plukenetia volubilis</i> L.) Oil Improves Hepatic Insulin Sensitivity and Glucose Metabolism through Insulin Signaling Pathway in a Rat Model of Type 2 Diabetes.

Sacha Inchi (<i>Plukenetia volubilis</i> L.) Oil Improves Hepatic Insulin Sensitivity and Glucose Metabolism through Insulin Signaling Pathway in a Rat Model of Type 2 Diabetes.

Sacha Inchi (Plukenetia volubilis L.) Oil Improves Hepatic Insulin Sensitivity and Glucose Metabolism through Insulin Signaling Pathway in a Rat Model of Type 2 Diabetes.

This study aimed to evaluate the role of sacha inchi oil (SI) in alleviating hepatic insulin resistance and improving glucose metabolism by inhibiting oxidative stress and inflammation in a rat model of type 2 diabetes. This model was established by providing a high-fat diet and streptozotocin to the rats, thereby inducing diabetes. The diabetic rats were treated orally with 0.5, 1, and 2 mL/kg body weight (b.w.) of SI or 30 mg/kg b.w. of pioglitazone daily for 5 weeks. Blood and hepatic tissues were used for insulin sensitivity, carbohydrate metabolism, oxidative stress, and inflammatory status assessment. Treatment with SI attenuated hyperglycemia and insulin resistance indices, and improved hepatic histopathological alterations in the diabetic rats in a dose-dependent manner, which is correlated with the decreased serum levels of the liver enzymes, alanine transaminase and aspartate transaminase. SI significantly diminished the hepatic oxidative status of the diabetic rats by inhibiting malondialdehyde and enhancing the antioxidant superoxide dismutase, catalase, and glutathione peroxidase activities. Moreover, pro-inflammatory cytokine levels, including tumor necrosis factor-α and interleukin-6, in the liver of the diabetic rats were significantly decreased by the SI. Furthermore, SI treatment enhanced the hepatic insulin sensitivity of the diabetic rats, as shown by the increased insulin receptor substrate-1 and p-Akt protein expression, decreased phosphoenolpyruvate carboxykinase-1 and glucose-6-phospatase protein expression, and increased hepatic glycogen content. Overall, these findings suggest that SI exerts a potential hepatic insulin-sensitizing effect and an improvement in glucose metabolism in the type 2 diabetic rats, at least in part through enhancing insulin signaling, antioxidant defense, and inhibiting inflammation.

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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
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35
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