Sophia Bowman-Derrick, Tegan M Harris, Jemima Beissbarth, Mariana Kleinecke, Katrina Lawrence, Teresa M Wozniak, Amy Bleakley, Angela Rumaseb, Michael J Binks, Robyn L Marsh, Peter S Morris, Amanda J Leach, Heidi Smith-Vaughan
{"title":"非类型流感嗜血杆菌带菌监测数据能否推断出中耳炎的抗菌药耐药性？","authors":"Sophia Bowman-Derrick, Tegan M Harris, Jemima Beissbarth, Mariana Kleinecke, Katrina Lawrence, Teresa M Wozniak, Amy Bleakley, Angela Rumaseb, Michael J Binks, Robyn L Marsh, Peter S Morris, Amanda J Leach, Heidi Smith-Vaughan","doi":"10.1002/ped4.12364","DOIUrl":null,"url":null,"abstract":"<p><strong>Importance: </strong>In remote communities of the Northern Territory, Australia, children experience high rates of otitis media (OM), commonly caused by non-typeable <i>Haemophilus influenzae</i> (NTHi). Few data exist on antibiotic susceptibility of NTHi from OM.</p><p><strong>Objective: </strong>To determine whether population-level nasopharyngeal NTHi antibiotic susceptibility data could inform antibiotic treatment for OM.</p><p><strong>Methods: </strong>NTHi isolates (<i>n</i> = 92) collected from ear discharge between 2003 and 2013 were selected to time- and age-match NTHi isolates from the nasopharyngeal carriage (<i>n</i> = 95). Antimicrobial susceptibility were tested. Phylogenomic trees and a genome-wide association study (GWAS) were performed to determine the similarity of nasopharyngeal and ear isolates at a population level.</p><p><strong>Results: </strong>Among 174 NTHi isolates available for antimicrobial susceptibility testing, 10.3% (18/174) were resistant to ampicillin and 9.2% (16/174) were resistant to trimethoprim-sulfamethoxazole. Small numbers of isolates (≤3) were resistant to tetracycline, chloramphenicol, or amoxicillin-clavulanic acid. There was no statistical difference in the proportion of ampicillin-resistant (<i>P</i> = 0.11) or trimethoprim-sulfamethoxazole-resistant isolates (<i>P</i> = 0.70) between ear discharge and nasopharynx-derived NTHi isolates. Three multi-drug resistant NTHi isolates were identified. Phylogenomic trees showed no clustering of 187 <i>Haemophilus influenzae</i> isolates based on anatomical niche (nasopharynx or ear discharge), and no genetic variations that distinguished NTHi derived from ear discharge and nasopharyngeal carriage were evident in the GWAS.</p><p><strong>Interpretation: </strong>In this population-level study, nasopharyngeal and ear discharge isolates did not represent distinct microbial populations. These results support tracking of population-level nasopharyngeal NTHi antibiotic resistance patterns to inform clinical management of OM in this population.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/ec/PED4-7-13.PMC10030701.pdf","citationCount":"0","resultStr":"{\"title\":\"Can non-typeable <i>Haemophilus influenzae</i> carriage surveillance data infer antimicrobial resistance associated with otitis media?\",\"authors\":\"Sophia Bowman-Derrick, Tegan M Harris, Jemima Beissbarth, Mariana Kleinecke, Katrina Lawrence, Teresa M Wozniak, Amy Bleakley, Angela Rumaseb, Michael J Binks, Robyn L Marsh, Peter S Morris, Amanda J Leach, Heidi Smith-Vaughan\",\"doi\":\"10.1002/ped4.12364\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Importance: </strong>In remote communities of the Northern Territory, Australia, children experience high rates of otitis media (OM), commonly caused by non-typeable <i>Haemophilus influenzae</i> (NTHi). Few data exist on antibiotic susceptibility of NTHi from OM.</p><p><strong>Objective: </strong>To determine whether population-level nasopharyngeal NTHi antibiotic susceptibility data could inform antibiotic treatment for OM.</p><p><strong>Methods: </strong>NTHi isolates (<i>n</i> = 92) collected from ear discharge between 2003 and 2013 were selected to time- and age-match NTHi isolates from the nasopharyngeal carriage (<i>n</i> = 95). Antimicrobial susceptibility were tested. Phylogenomic trees and a genome-wide association study (GWAS) were performed to determine the similarity of nasopharyngeal and ear isolates at a population level.</p><p><strong>Results: </strong>Among 174 NTHi isolates available for antimicrobial susceptibility testing, 10.3% (18/174) were resistant to ampicillin and 9.2% (16/174) were resistant to trimethoprim-sulfamethoxazole. Small numbers of isolates (≤3) were resistant to tetracycline, chloramphenicol, or amoxicillin-clavulanic acid. There was no statistical difference in the proportion of ampicillin-resistant (<i>P</i> = 0.11) or trimethoprim-sulfamethoxazole-resistant isolates (<i>P</i> = 0.70) between ear discharge and nasopharynx-derived NTHi isolates. Three multi-drug resistant NTHi isolates were identified. Phylogenomic trees showed no clustering of 187 <i>Haemophilus influenzae</i> isolates based on anatomical niche (nasopharynx or ear discharge), and no genetic variations that distinguished NTHi derived from ear discharge and nasopharyngeal carriage were evident in the GWAS.</p><p><strong>Interpretation: </strong>In this population-level study, nasopharyngeal and ear discharge isolates did not represent distinct microbial populations. 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Can non-typeable Haemophilus influenzae carriage surveillance data infer antimicrobial resistance associated with otitis media?
Importance: In remote communities of the Northern Territory, Australia, children experience high rates of otitis media (OM), commonly caused by non-typeable Haemophilus influenzae (NTHi). Few data exist on antibiotic susceptibility of NTHi from OM.
Objective: To determine whether population-level nasopharyngeal NTHi antibiotic susceptibility data could inform antibiotic treatment for OM.
Methods: NTHi isolates (n = 92) collected from ear discharge between 2003 and 2013 were selected to time- and age-match NTHi isolates from the nasopharyngeal carriage (n = 95). Antimicrobial susceptibility were tested. Phylogenomic trees and a genome-wide association study (GWAS) were performed to determine the similarity of nasopharyngeal and ear isolates at a population level.
Results: Among 174 NTHi isolates available for antimicrobial susceptibility testing, 10.3% (18/174) were resistant to ampicillin and 9.2% (16/174) were resistant to trimethoprim-sulfamethoxazole. Small numbers of isolates (≤3) were resistant to tetracycline, chloramphenicol, or amoxicillin-clavulanic acid. There was no statistical difference in the proportion of ampicillin-resistant (P = 0.11) or trimethoprim-sulfamethoxazole-resistant isolates (P = 0.70) between ear discharge and nasopharynx-derived NTHi isolates. Three multi-drug resistant NTHi isolates were identified. Phylogenomic trees showed no clustering of 187 Haemophilus influenzae isolates based on anatomical niche (nasopharynx or ear discharge), and no genetic variations that distinguished NTHi derived from ear discharge and nasopharyngeal carriage were evident in the GWAS.
Interpretation: In this population-level study, nasopharyngeal and ear discharge isolates did not represent distinct microbial populations. These results support tracking of population-level nasopharyngeal NTHi antibiotic resistance patterns to inform clinical management of OM in this population.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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