新型核桃粕弹性酶抑制肽的制备、鉴定及分子对接

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY
Yu Xiong , Peng Peng , Shi-Jia Chen, Min Chang, Qian Wang, Sheng-Nan Yin, Di-Feng Ren
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引用次数: 2

摘要

本研究旨在通过超声波酶法从核桃粕中分离出具有弹性酶抑制活性的生物活性肽。利用响应面法(RSM)确定了核桃粕蛋白水解物(WMPHs)的最佳水解条件,并利用LC-MS/MS对分子量为3kda的核桃粕蛋白水解物进行了分析,共鉴定出556个肽段。PyRx虚拟筛选和Autodock Vina分子对接表明,五肽Phe-Phe-Val-Pro-Phe (FFVPF)主要通过疏水相互作用、氢键和π-硫键与弹性酶相互作用,结合能为−5.22 kcal/mol。抑制活性验证结果表明,FFVPF具有较好的弹性酶抑制活性,IC50值为0.469±0.01 mg/mL。此外,FFVPF在胃环境中表现出特定的稳定性。这些发现表明,从脱脂核桃粕中提取的五肽FFVPF可以作为食品、医疗和化妆品行业中弹性酶抑制剂的潜在来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preparation, identification, and molecular docking of novel elastase inhibitory peptide from walnut (Juglans regia L.) meal

This study aimed to isolate bioactive peptides with elastase inhibitory activity from walnut meal via ultrasonic enzymatic hydrolysis. The optimal hydrolysis conditions of walnut meal protein hydrolysates (WMPHs) were obtained by response surface methodology (RSM), while a molecular weight of<3 kDa fraction was analyzed by LC-MS/MS, and 556 peptides were identified. PyRx virtual screening and Autodock Vina molecular docking revealed that the pentapeptide Phe-Phe-Val-Pro-Phe (FFVPF) could interact with elastase primarily through hydrophobic interactions, hydrogen bonds, and π-sulfur bonds, with a binding energy of −5.22 kcal/mol. The verification results of inhibitory activity showed that FFVPF had better elastase inhibitory activity, with IC50 values of 0.469 ± 0.01 mg/mL. Furthermore, FFVPF exhibited specific stability in the gastric environment. These findings suggest that the pentapeptide FFVPF from defatted walnut meal could serve as a potential source of elastase inhibitors in the food, medical, and cosmetics industries.

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来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
83
审稿时长
82 days
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