研究秋葵(Abelmoschus esculentus)作为降胆固醇、抗炎和抗氧化食品的体外作用模式

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY
Giovanni Panighel , Irene Ferrarese , Maria Giovanna Lupo , Stefania Sut , Stefano Dall'Acqua , Nicola Ferri
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引用次数: 1

摘要

秋葵(Abelmoschus esculentus)是最近才作为食物引入欧洲的。它原产于印度,是尼日利亚最重要的蔬菜之一。叶子可以食用,但果实也富含营养和生物活性化合物(即膳食纤维、维生素、油脂、多糖、多酚),并有几种促进健康的作用,包括降脂特性。在本研究中,我们研究了水果和叶子提取物对人肝癌细胞系Huh7中胆固醇代谢的关键介质,即低密度脂蛋白受体(LDLR)和蛋白转化酶枯草杆菌素/激酶9型(PCSK9)表达的影响。此外,研究了thp1来源的巨噬细胞对促炎细胞因子(IL-1β、IL-6和TNF-α)表达的影响,以评估其潜在的抗炎作用。秋葵果提取物对LDLR mRNA和蛋白表达的影响分别为1.4±0.3倍和4.8±1.5倍,而PCSK9的表达未见明显改变。此外,果实提取物对胆酸具有显著的固存能力。叶丁醇提取物对LDLR(+3.1±1.6倍)和PCSK9(+1.3±0.4倍)的表达均有诱导作用。结果表明,叶丁醇提取物具有显著的抗炎作用,其IL-1β mRNA水平(与对照组相比为- 28±8%)、IL-6 mRNA水平(与对照组相比为- 11±1%)和TNF-α mRNA水平(与对照组相比为- 43±8%)均显著降低,而果实提取物无抗炎活性。最后,叶乙酸乙酯提取物具有与抗坏血酸相当的抗氧化能力。综上所述,我们提供的证据表明,叶丁醇提取物和更有效的果提取物诱导了LDLR的表达,这可能解释了之前报道的秋葵降低胆固醇的作用。此外,秋葵提取物降低了thp1来源的巨噬细胞中促炎细胞因子的表达,这可能表明秋葵具有血管保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating the in vitro mode of action of okra (Abelmoschus esculentus) as hypocholesterolemic, anti-inflammatory, and antioxidant food

Okra (Abelmoschus esculentus) have been introduced as food relatively recently in Europe. It is native to India and one of the most important vegetables in Nigeria. The leaves can be consumed but also the fruit is rich in nutrients and bioactive compounds (i.e., dietary fiber, vitamins, oils, polysaccharides, polyphenols) and several health promoting actions have been ascribed, including a lipid-lowering properties. In this work the effects of fruit and leaf extracts on expression of key mediators of cholesterol metabolism, i.e., the low-density lipoprotein receptor (LDLR) and proprotein convertase subtilisin/kexin type 9 (PCSK9), were investigated in human hepatoma cell line Huh7. Furthermore, effects on proinflammatory cytokines (IL-1β, IL-6 and TNF-α) expressed by THP1-derived macrophages were studied to assess potential anti-inflammatory actions.

Okra fruit extract significantly induced the mRNA and protein levels of the LDLR by 1.4 ± 0.3 and 4.8 ± 1.5-fold, respectively without any significant modification of PCSK9 expression. In addition, fruit extract showed a significant sequestering ability of cholic acid. Leaf butanol extract exerted similar action by inducing the expression of both the LDLR (+3.1 ± 1.6-fold vs control) and PCSK9 (+1.3 ± 0.4-fold vs control). The evaluation of the potential anti-inflammatory effect revealed a significant action of leaf butanol extract with reduced mRNA levels of IL-1β (−28 ± 8 % vs control), IL-6 (−11 ± 1 % vs control) and TNF-α (−43 ± 8 % vs control), while fruit extract did not show any anti-inflammatory activity. Finally, leaf ethyl acetate extract showed a significant antioxidant capacity comparable to ascorbic acid. Taken together, we provided evidence that leaf butanol extract and, more effectively, fruit extract induced the LDLR expression, effect that may explain the previously reported hypocholesterolemic action of okra. In addition, okra’s extracts reduced the expression of pro-inflammatory cytokines from THP1-derived macrophages, an effect that may suggest a vascular protective action of okra.

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来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
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0.00%
发文量
83
审稿时长
82 days
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