W Bergthaller, B H Dijkink, H C Langelaan, J M Vereijken
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引用次数: 12
摘要
淀粉生物合成代谢背景不同的某些豌豆基因型（野生型和 5 r 突变体）的子叶材料的干分离行为，只有平滑播种基因型的蛋白质回收率才能令人满意。结构效应和蛋白质分布（淀粉颗粒大小分布、非淀粉部分的蛋白质）起着重要作用。根据预期，蛋白质成分在干分离过程中保持不变。与所使用的基因型无关，在湿分离过程中使用的条件只允许回收 50%的种子蛋白质，而大约 10%的蛋白质随纤维流失，40%的蛋白质随不明工艺水流流失。在等电沉淀过程中，球状的维吉林蛋白仍保持溶解状态，并随废水排出。在碱性条件下，分离物的溶解度很低，即使是圆籽品种奥达利特（Odalett）也是如此。Odalett（0.4% 至 0.7%）。
Protein from pea mutants as a co-product in starch separation--isolates from wet and dry separation: yield, composition and solubility.
The dry separation behaviour of cotyledon material of certain pea genotypes (wild type and 5 r mutants) with different metabolic background in starch biosynthesis allowed satisfying protein recovery only with smooth seeded genotypes. Structural effects and protein distribution (starch granule size distribution, protein in non-starch part) play an important role. According to expectations protein composition remained unaltered during dry separation procedures. Independent of genotypes used the applied conditions in wet separation allowed to recover just 50% of seed proteins while approximately 10% got lost with fibres and 40% with unspecified process water streams. Globular vicilins remained dissolved during iso-electric precipitation and were discarded with waste water streams. The solubility of isolates was rather low under alkaline conditions, even for the round seeded cv. Odalett (0.4 to 0.7% dry substance).
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