J. Schoelz, D. Volenberg, M. Adhab, Zhiwei Fang, V. Klassen, C. Spinka, M. Al Rwahnih
{"title":"在密苏里种植的葡萄品种中发现的病毒调查","authors":"J. Schoelz, D. Volenberg, M. Adhab, Zhiwei Fang, V. Klassen, C. Spinka, M. Al Rwahnih","doi":"10.5344/ajev.2020.20043","DOIUrl":null,"url":null,"abstract":"In 2017 a survey was initiated of vineyards throughout Missouri for the presence of 19 different grapevine viruses. A total of 400 samples were collected from 25 grape cultivars, with a sample consisting of 16 petioles (four petioles from four different vines). Sampling within vineyard blocks was random, without regard to the presence or absence of symptoms. After nucleic acids were extracted from petiole samples, nucleic acid samples were analyzed using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) to detect viral RNA/DNA. At least one virus was detected in 90% of the samples. The most common virus detected in the survey was grapevine rupestris stem pitting-associated virus, which was found in 59% of the samples, followed by grapevine leafroll-associated virus 3 (53%), grapevine red blotch virus (35%), grapevine virus E (31%), grapevine leafroll-associated virus 2 (19%), grapevine virus B (17%), grapevine fleck virus (13.5%), grapevine leafroll-associated virus-2 Redglobe (9%), grapevine vein clearing virus (8%), grapevine virus A (0.5%), and grapevine leafroll-associated virus 4 strain 5 (0.2%). Two or more viruses were detected in ~65% of the samples, with an upper limit of seven viruses detected in one sample. An analysis of the survey results indicated that each grapevine cultivar appears to be infected by specific virus combinations that are, for the most part, unique to that cultivar.","PeriodicalId":7461,"journal":{"name":"American Journal of Enology and Viticulture","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5344/ajev.2020.20043","citationCount":"10","resultStr":"{\"title\":\"A Survey of Viruses Found in Grapevine Cultivars Grown in Missouri\",\"authors\":\"J. Schoelz, D. Volenberg, M. Adhab, Zhiwei Fang, V. Klassen, C. Spinka, M. Al Rwahnih\",\"doi\":\"10.5344/ajev.2020.20043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In 2017 a survey was initiated of vineyards throughout Missouri for the presence of 19 different grapevine viruses. A total of 400 samples were collected from 25 grape cultivars, with a sample consisting of 16 petioles (four petioles from four different vines). Sampling within vineyard blocks was random, without regard to the presence or absence of symptoms. After nucleic acids were extracted from petiole samples, nucleic acid samples were analyzed using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) to detect viral RNA/DNA. At least one virus was detected in 90% of the samples. The most common virus detected in the survey was grapevine rupestris stem pitting-associated virus, which was found in 59% of the samples, followed by grapevine leafroll-associated virus 3 (53%), grapevine red blotch virus (35%), grapevine virus E (31%), grapevine leafroll-associated virus 2 (19%), grapevine virus B (17%), grapevine fleck virus (13.5%), grapevine leafroll-associated virus-2 Redglobe (9%), grapevine vein clearing virus (8%), grapevine virus A (0.5%), and grapevine leafroll-associated virus 4 strain 5 (0.2%). Two or more viruses were detected in ~65% of the samples, with an upper limit of seven viruses detected in one sample. An analysis of the survey results indicated that each grapevine cultivar appears to be infected by specific virus combinations that are, for the most part, unique to that cultivar.\",\"PeriodicalId\":7461,\"journal\":{\"name\":\"American Journal of Enology and Viticulture\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2020-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.5344/ajev.2020.20043\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American Journal of Enology and Viticulture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.5344/ajev.2020.20043\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Enology and Viticulture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5344/ajev.2020.20043","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
A Survey of Viruses Found in Grapevine Cultivars Grown in Missouri
In 2017 a survey was initiated of vineyards throughout Missouri for the presence of 19 different grapevine viruses. A total of 400 samples were collected from 25 grape cultivars, with a sample consisting of 16 petioles (four petioles from four different vines). Sampling within vineyard blocks was random, without regard to the presence or absence of symptoms. After nucleic acids were extracted from petiole samples, nucleic acid samples were analyzed using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) to detect viral RNA/DNA. At least one virus was detected in 90% of the samples. The most common virus detected in the survey was grapevine rupestris stem pitting-associated virus, which was found in 59% of the samples, followed by grapevine leafroll-associated virus 3 (53%), grapevine red blotch virus (35%), grapevine virus E (31%), grapevine leafroll-associated virus 2 (19%), grapevine virus B (17%), grapevine fleck virus (13.5%), grapevine leafroll-associated virus-2 Redglobe (9%), grapevine vein clearing virus (8%), grapevine virus A (0.5%), and grapevine leafroll-associated virus 4 strain 5 (0.2%). Two or more viruses were detected in ~65% of the samples, with an upper limit of seven viruses detected in one sample. An analysis of the survey results indicated that each grapevine cultivar appears to be infected by specific virus combinations that are, for the most part, unique to that cultivar.
期刊介绍:
The American Journal of Enology and Viticulture (AJEV), published quarterly, is an official journal of the American Society for Enology and Viticulture (ASEV) and is the premier journal in the English language dedicated to scientific research on winemaking and grapegrowing. AJEV publishes full-length research papers, literature reviews, research notes, and technical briefs on various aspects of enology and viticulture, including wine chemistry, sensory science, process engineering, wine quality assessments, microbiology, methods development, plant pathogenesis, diseases and pests of grape, rootstock and clonal evaluation, effect of field practices, and grape genetics and breeding. All papers are peer reviewed, and authorship of papers is not limited to members of ASEV. The science editor, along with the viticulture, enology, and associate editors, are drawn from academic and research institutions worldwide and guide the content of the Journal.