小鼠肾脏和肝脏的扫描透射软x射线光谱显微镜

IF 1.8 4区物理与天体物理 Q2 SPECTROSCOPY

Journal of Electron Spectroscopy and Related Phenomena Pub Date : 2023-07-01 DOI:10.1016/j.elspec.2023.147368

Tuomas Mansikkala , Takuji Ohigashi , Miia H. Salo , Anniina E. Hiltunen , Reetta Vuolteenaho , Petra Sipilä , Satu Kuure , Marko Huttula , Johanna Uusimaa , Reetta Hinttala , Ilkka Miinalainen , Salla Kangas , Minna Patanen

{"title":"小鼠肾脏和肝脏的扫描透射软x射线光谱显微镜","authors":"Tuomas Mansikkala , Takuji Ohigashi , Miia H. Salo , Anniina E. Hiltunen , Reetta Vuolteenaho , Petra Sipilä , Satu Kuure , Marko Huttula , Johanna Uusimaa , Reetta Hinttala , Ilkka Miinalainen , Salla Kangas , Minna Patanen","doi":"10.1016/j.elspec.2023.147368","DOIUrl":null,"url":null,"abstract":"<div><p>Scanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified <em>Slc17a5</em> mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge.</p></div>","PeriodicalId":15726,"journal":{"name":"Journal of Electron Spectroscopy and Related Phenomena","volume":"266 ","pages":"Article 147368"},"PeriodicalIF":1.8000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver\",\"authors\":\"Tuomas Mansikkala , Takuji Ohigashi , Miia H. Salo , Anniina E. Hiltunen , Reetta Vuolteenaho , Petra Sipilä , Satu Kuure , Marko Huttula , Johanna Uusimaa , Reetta Hinttala , Ilkka Miinalainen , Salla Kangas , Minna Patanen\",\"doi\":\"10.1016/j.elspec.2023.147368\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Scanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified <em>Slc17a5</em> mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge.</p></div>\",\"PeriodicalId\":15726,\"journal\":{\"name\":\"Journal of Electron Spectroscopy and Related Phenomena\",\"volume\":\"266 \",\"pages\":\"Article 147368\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Electron Spectroscopy and Related Phenomena\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0368204823000853\",\"RegionNum\":4,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Electron Spectroscopy and Related Phenomena","FirstCategoryId":"101","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0368204823000853","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"SPECTROSCOPY","Score":null,"Total":0}

引用次数: 0

摘要

扫描透射x射线显微镜(STXM)在软x射线范围内非常适合研究哺乳动物软组织的超微结构特征。特别是在碳1s边缘，由于组织样品中存在的官能团的x射线吸收截面的快速变化，使得无标记软x射线光谱显微镜研究的成像对比度在边缘上变化很大。我们展示了小鼠肾脏和肝脏组织的STXM光谱显微镜成像。我们特别关注转基因Slc17a5小鼠的超微结构异常。STXM是一种很有前途的技术，可以在不受染色剂影响的情况下研究贮藏病，但样品制备存在挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver

Scanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified Slc17a5 mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Electron Spectroscopy and Related Phenomena 物理-光谱学

CiteScore

3.30

自引率

5.30%

发文量

审稿时长

60 days

期刊介绍： The Journal of Electron Spectroscopy and Related Phenomena publishes experimental, theoretical and applied work in the field of electron spectroscopy and electronic structure, involving techniques which use high energy photons (>10 eV) or electrons as probes or detected particles in the investigation.