泰国北部草药提取物抗氧化、抗衰老和抗微生物活性的筛选与表征

IF 1.3 Q4 FOOD SCIENCE & TECHNOLOGY
Chakkraphong Khonthun, Krissana Khoothiam, Orada Chumphukam, Rungthip Thongboontho, Panida Oonlao, P. Nuntaboon, Kanokkarn Phromnoi
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引用次数: 1

摘要

背景:衰老是一个由氧化剂和衰老相关酶引起的过程。因此,对这些过程的抑制会加剧抗衰老剂的作用。本研究旨在评估泰国传统草药中使用的七种草药的叶提取物的抗氧化和抗衰老活性。方法:研究人员使用比色法评估酚类物质(TPC)、抗氧化剂、抗胶原酶和抗酪氨酸酶活性的总水平。细胞毒性作用用MTT法测定,基质金属蛋白酶(MMPs)分泌用明胶酶谱法评估。此外,采用圆盘扩散法和肉汤微量稀释法检测了对微生物生长的抑制作用。结果:TPC在48.68–440.91 mg GAE/g乙醇叶提取物之间。石榴皮、余甘子、番石榴、贝利卡和切布对ABTS自由基具有较高的抗氧化活性,而珙桐、番石榴和石榴皮对DPPH具有较高的中和作用。FRAP测定显著降低了T.chebula和P.granatum的能力。T.chebula和P.guajava对H2O2诱导的ROS产生表现出最高的抑制作用。余甘子、番石榴、贝利卡和嗜潮杆菌降低了酪氨酸酶和胶原酶的活性。P.gaujava、T.chebula和T.bellrica显示抑制成纤维细胞分泌MMP-2。所有浓度的叶提取物对成纤维细胞都是无毒的。granatum和T.bellrica可抑制痤疮杆菌、大肠杆菌和金黄色葡萄球菌的生长。结论:初步研究表明,石榴皮和水曲柳叶提取物具有抗氧化、抗衰老和抗菌活性,是适合化妆品使用的有效成分。关键词:抗氧化剂,抗衰老,叶提取物
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening and characterization of antioxidant, anti-aging, and anti-microbial activity of herbal extracts in Northern Thailand
Background: Aging is a process caused by oxidants and aging-related enzymes. Therefore, the inhibition of these processes can exacerbate anti-aging agents. This study aimed to evaluate the antioxidant and anti-aging activities of leaf extracts from seven herbs used in traditional Thai herbal remedies. Methods: Researchers assessed the total levels of phenolic content (TPC), antioxidant, anti-collagenase, and anti-tyrosinase activity using colorimetric methods. Cytotoxicity effects were determined using MTT assays, and matrix metalloproteinases(MMPs) secretion was assessed through gelatin zymography. In addition, inhibitory effects on the growth of microorganisms were examined using the disc diffusion and broth microdilution method.Results: TPC ranged between 48.68–440.91 mg GAE/g of ethanolic leaf extracts. High antioxidant activities against ABTS radicals were detected in P. granatum, P. emblica, P. guajava, T. bellirica, and T. chebula, while high DPPH neutralization appeared in M. coreia, P. guajava, and P. granatum. FRAP assays significantly reduced the power of T. chebula and P. granatum. T. chebula and P. guajava exhibited the highest inhibitory effect on H2O2induced ROS production. P. emblica, P. guajava, T. bellirica, and E. hygrophilus reduced tyrosinase and collagenase activities.  P. gaujava, T. chebula, and T. bellirica were shown to inhibit the secretion of MMP-2 from fibroblast cells. All concentrations of leaf extracts were non-toxic to fibroblast cells. P.granatum and T. bellirica could inhibit the growth of P. acnes, E. coli, and S. aureus. Conclusion: Preliminary studies showed that P.granatum and T. bellirica leaf extracts have antioxidants, anti-aging, and anti-bacterial activities, as well as active ingredients suitable for cosmetic products.Keywords: antioxidants, anti-aging, leaf extracts
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来源期刊
Functional Foods in Health and Disease
Functional Foods in Health and Disease FOOD SCIENCE & TECHNOLOGY-
CiteScore
2.20
自引率
20.00%
发文量
47
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