Qi Zhang, Shukun Wang, Bin Qin, Hao-yue Sun, Xian-kai Yuan, Qi Wang, Junjie Xu, Zhengong Yin, Yan-li Du, Ji-dao Du, Caihua Li
{"title":"通过转录组学和代谢组学分析,揭示了豆芽期苯丙素对盐胁迫的响应机制","authors":"Qi Zhang, Shukun Wang, Bin Qin, Hao-yue Sun, Xian-kai Yuan, Qi Wang, Junjie Xu, Zhengong Yin, Yan-li Du, Ji-dao Du, Caihua Li","doi":"10.1002/fes3.481","DOIUrl":null,"url":null,"abstract":"<p>Common bean (<i>Phaseolus vulgaris</i>) seeds are important legume crops and an important source of dietary proteins and carbohydrates. Therefore, it is important to develop strategies to improve salt tolerance in common beans. In this study, transcriptome and metabolome analyses were conducted on local common bean variety under salt stress at the sprout stage for a period of 0, 12, and 24 h. Results showed that phenylpropanoid pathways (including phenylpropanoid biosynthesis and phenylalanine metabolism) and flavonoid pathways (including flavonoid biosynthesis and flavone and flavonol biosynthesis) played an important role in controlling responses to salt stress as evidenced by analysis of differentially expression genes, common expression patterns, WCGNA, and differentially altered metabolites (DAMs) analyses. In addition, exploration of the activities of 4-coumarate-CoA ligase (4CL), caffeoyl-CoA O-methyltransferase (CCoAOMT), peroxidase (POD), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and flavonol synthase (FLS) further showed that phenylpropanoid and flavonoid pathways participate in plant responses to salt stress. Moreover, the phenylpropanoid pathways and flavonoid pathways were found to be potential pathways regulating plant response to salt stress based on transcriptome and metabolome analysis. The activities of 4CL, CCoAOMT, POD, CHI, DFR, and FLS revealed that these pathways are crucial to the regulation of plant responses to salt stress. These findings provided theoretical basis for further improvement of salt tolerance in common bean.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.481","citationCount":"0","resultStr":"{\"title\":\"Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean (Phaseolus vulgaris) responding to salt stress at sprout stage\",\"authors\":\"Qi Zhang, Shukun Wang, Bin Qin, Hao-yue Sun, Xian-kai Yuan, Qi Wang, Junjie Xu, Zhengong Yin, Yan-li Du, Ji-dao Du, Caihua Li\",\"doi\":\"10.1002/fes3.481\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Common bean (<i>Phaseolus vulgaris</i>) seeds are important legume crops and an important source of dietary proteins and carbohydrates. Therefore, it is important to develop strategies to improve salt tolerance in common beans. In this study, transcriptome and metabolome analyses were conducted on local common bean variety under salt stress at the sprout stage for a period of 0, 12, and 24 h. Results showed that phenylpropanoid pathways (including phenylpropanoid biosynthesis and phenylalanine metabolism) and flavonoid pathways (including flavonoid biosynthesis and flavone and flavonol biosynthesis) played an important role in controlling responses to salt stress as evidenced by analysis of differentially expression genes, common expression patterns, WCGNA, and differentially altered metabolites (DAMs) analyses. In addition, exploration of the activities of 4-coumarate-CoA ligase (4CL), caffeoyl-CoA O-methyltransferase (CCoAOMT), peroxidase (POD), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and flavonol synthase (FLS) further showed that phenylpropanoid and flavonoid pathways participate in plant responses to salt stress. Moreover, the phenylpropanoid pathways and flavonoid pathways were found to be potential pathways regulating plant response to salt stress based on transcriptome and metabolome analysis. The activities of 4CL, CCoAOMT, POD, CHI, DFR, and FLS revealed that these pathways are crucial to the regulation of plant responses to salt stress. 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Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean (Phaseolus vulgaris) responding to salt stress at sprout stage
Common bean (Phaseolus vulgaris) seeds are important legume crops and an important source of dietary proteins and carbohydrates. Therefore, it is important to develop strategies to improve salt tolerance in common beans. In this study, transcriptome and metabolome analyses were conducted on local common bean variety under salt stress at the sprout stage for a period of 0, 12, and 24 h. Results showed that phenylpropanoid pathways (including phenylpropanoid biosynthesis and phenylalanine metabolism) and flavonoid pathways (including flavonoid biosynthesis and flavone and flavonol biosynthesis) played an important role in controlling responses to salt stress as evidenced by analysis of differentially expression genes, common expression patterns, WCGNA, and differentially altered metabolites (DAMs) analyses. In addition, exploration of the activities of 4-coumarate-CoA ligase (4CL), caffeoyl-CoA O-methyltransferase (CCoAOMT), peroxidase (POD), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and flavonol synthase (FLS) further showed that phenylpropanoid and flavonoid pathways participate in plant responses to salt stress. Moreover, the phenylpropanoid pathways and flavonoid pathways were found to be potential pathways regulating plant response to salt stress based on transcriptome and metabolome analysis. The activities of 4CL, CCoAOMT, POD, CHI, DFR, and FLS revealed that these pathways are crucial to the regulation of plant responses to salt stress. These findings provided theoretical basis for further improvement of salt tolerance in common bean.
期刊介绍:
Food and Energy Security seeks to publish high quality and high impact original research on agricultural crop and forest productivity to improve food and energy security. It actively seeks submissions from emerging countries with expanding agricultural research communities. Papers from China, other parts of Asia, India and South America are particularly welcome. The Editorial Board, headed by Editor-in-Chief Professor Martin Parry, is determined to make FES the leading publication in its sector and will be aiming for a top-ranking impact factor.
Primary research articles should report hypothesis driven investigations that provide new insights into mechanisms and processes that determine productivity and properties for exploitation. Review articles are welcome but they must be critical in approach and provide particularly novel and far reaching insights.
Food and Energy Security offers authors a forum for the discussion of the most important advances in this field and promotes an integrative approach of scientific disciplines. Papers must contribute substantially to the advancement of knowledge.
Examples of areas covered in Food and Energy Security include:
• Agronomy
• Biotechnological Approaches
• Breeding & Genetics
• Climate Change
• Quality and Composition
• Food Crops and Bioenergy Feedstocks
• Developmental, Physiology and Biochemistry
• Functional Genomics
• Molecular Biology
• Pest and Disease Management
• Post Harvest Biology
• Soil Science
• Systems Biology