重新利用农业废弃物作为低成本的人造肉支架

L. Perreault, R. Thyden, Jack T. Kloster, Jordan D Jones, Jordan Nunes, Andriana A. Patmanidis, David Reddig, T. Dominko, G. Gaudette
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引用次数: 2

摘要

利用组织工程和生物生产技术(细胞农业)在体外培养肉类已成为应对全球粮食安全挑战的一种越来越有前途的解决方案。我们的实验室已经建立了在脱细胞植物上培养牛肌肉组织的方法,这是一种可行的低成本、可持续的方法,可以在可食用的支架上培养肉。这一领域的大部分工作都集中在利用具有内在经济价值的可食用植物材料(即菠菜叶、苹果、西兰花)。收获废料,如玉米壳或菠萝蜜代表丰富的纤维素来源的支架生产,可能是一个可行的替代品。本研究的目的是通过组织工程和生物生产技术,研究玉米壳和菠萝蜜果皮脱细胞可食用样品的培养肉生产。玉米皮和菠萝蜜皮浸泡脱细胞。DNA定量和组织学分析表明,玉米皮和菠萝蜜皮的脱细胞率分别为0.17±0.06和0.07±0.00 ug /g。脱细胞后,玉米壳骨架沿纤维排列方向的刚度从56.67±16.71 MPa降至12.95±2.43 MPa,菠萝蜜从7.54±2.42 MPa降至2.47±1.47 MPa。牛卫星细胞(BSCs)(11.45±2.24 ug/ul裂解物/克)和禽卫星细胞(QM7s)(12.90±1.99 ug/ul裂解物/克)的种子支架在菠萝蜜支架上的蛋白产量增加。在玉米外壳支架上培养的QM7蛋白产量增加,而在玉米外壳上播种的PBSCs蛋白含量不高于对照组(QM7在玉米外壳上的含量为16.28±3.55 ug/ul lysate/ g, PBSCs在玉米外壳上的含量为9.57±1.56 ug/ul lysate/ g,对照组为6.35±1.43 ug/ul lysate/ g)。此外,在动态环境下,在玉米壳支架上观察到细胞从支架到支架的转移(头到头转移)。这些结果表明,脱细胞的收获废物支架可能有助于实现培养肉制品,这将有助于更强大和环境可持续的食物供应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Repurposing agricultural waste as low-cost cultured meat scaffolds
Growing meat in vitro using tissue engineering and bioproduction techniques (cellular agriculture) has become an increasingly promising solution to the global food security challenge. Our lab has established methods to cultivate bovine muscle tissue on decellularized plants, representing a viable low-cost, sustainable method to grow meat on edible scaffolds. Most work in this area has focused on the use of edible plant materials (i.e., spinach leaves, apple, broccoli) with inherent economic value. Harvest waste such as corn husk or jackfruit represent abundant sources of cellulose for scaffold production and may be a viable alternative. The present study aims to investigate production of cultured meat through tissue engineering and bioproduction on decellularized, edible samples of corn husk and jackfruit rind. Corn husks and jackfruit rinds were exposed to immersion decellularization. DNA quantification and histological analysis demonstrated sufficient decellularization (0.17 ± 0.06 and 0.07 ± 0.00 ug DNA/g tissue for corn husk and jackfruit rinds, respectively). Following decellularization, corn husk scaffold stiffnesses decreased from 56.67±16.71 MPa to 12.95±2.43 MPa in fiber-aligned direction, while jackfruit decreased from 7.54 ±2.42 MPa to 2.47±1.47 MPa. Seeded scaffolds with bovine satellite cells (BSCs) (11.45±2.24 ug/ul lysate/Gram) and avian (QM7s) (12.90±1.99 ug/ul lysate/Gram) demonstrated increased protein yields on jackfruit scaffolds. QM7 cultured on corn husk scaffolds yielded increased protein but PBSCs seeded on corn husks did not yield protein content higher than controls (QM7 on corn husk: 16.28±3.55, PBSCs on corn husks: 9.57±1.56 ug/ul lysate/Gram, control: 6.35±1.43 ug/ul lysate/Gram). Additionally, cell transfer from scaffold to scaffold (bead-to-bead transfer) was observed on corn husk scaffolds in a dynamic environment. These results suggest that decellularized harvest waste scaffolds may aid in realization of cultured meat products that will contribute to a more robust and environmentally sustainable food supply.
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