Inhibitory Effects of Vernonia amygdalina Leaf Extracts on Free Radical Scavenging, Tyrosinase, and Amylase Activities.

Cytotoxicity and instability are the limitations when using bioactive compounds in cosmetic and pharmacology products. This study assesses Vernonia amygdalina leaf extracts for their antityrosinase, antiamylase, and antioxidant activities. Group A extracts were obtained using an aqueous solvent system [70% (v/v) of methanol (BTL70ME), ethanol (BTL70ET), and acetone (BTL70AC)]. Group B extracts were obtained using organic solvents of varying polarities. The results displayed that all extracts exhibited antityrosinase, antiamylase, and antioxidant activities in vitro. The most potent antityrosinase activity was observed in BTL70AC, with a half-maximal inhibitory concentration (IC₅₀) value of 20 μg/mL. BTL_Ethyl acetate and BTL70AC showed potential antiamylase activity. BTL_Isopropanol and BTL_Ethanol exhibited potential antioxidant activity, with IC₅₀ values of 4.0 μg/mL. The total phenolic content of BTL70ME, BTL70ET, and BTL70AC was 72.29±14.14, 65.98±11.91, and 69.37±7.72 mg gallic acid/g extract, respectively. The total flavonoid content was 53.04±5.22, 44.35±13.17, and 61.74±13.17 mg quercetin/g extract, respectively. Group A extracts contained polyphenols, flavonoids, saponins, terpenoids, steroids, and cardiac glycosides. These biological properties can potentially be attributed to the types and quantities of phytochemicals present. Bioactive compounds in the extracts may exert synergistic effects in vitro by interfering with the conformational changes of tyrosinase during substrate binding. Both groups of extracts have the potential to suppress biomolecule degradation, promote antiaging and antimelasma effects, and their phytochemicals can help lower blood glucose levels in diabetes.