使用一次性滤芯条和基于半导体的生物传感器平台快速检测 SARS-CoV-2 病毒。

IF 1.5 4区 工程技术 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC
Minghan Xian, Hao Luo, Xinyi Xia, Chaker Fares, Patrick H Carey, Chan-Wen Chiu, Fan Ren, Siang-Sin Shan, Yu-Te Liao, Shu-Min Hsu, Josephine F Esquivel-Upshaw, Chin-Wei Chang, Jenshan Lin, Steven C Ghivizzani, Stephen J Pearton
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引用次数: 0

摘要

利用一次性生物功能化功能带实现了对 SARS-CoV-2 尖峰蛋白和灭活病毒的检测,这些功能带可连接到可重复使用的印刷电路板外部,通过嵌入式金属氧化物半导体场效应晶体管(MOSFET)进行信号放大。通过一系列化学反应,将单克隆抗体和多克隆抗体固定在用作传感表面的镀金电极上。生物功能化的一个重要步骤,即在传感器条上形成镀金簇,是通过扫描电子显微镜和电学测量来验证的,以确认硫醇基团成功结合在金表面上。功能化传感器与 MOSFET 的栅极外部连接,同步脉冲同时作用于传感条和 MOSFET 的漏极触点。由此产生的漏极波形动态变化被转换成模拟电压和数字读数,与测试溶液中的蛋白质和病毒浓度相关联。使用这两种抗体功能化的传感器可检测的蛋白质浓度范围很广,从 1 fg/ml 到 10 μg/ml,病毒浓度范围从 100 到 2500 PFU/ml。结果表明,这种方法具有开发便携式、低成本和一次性盒式传感器系统的潜力,可用于病毒性疾病的床旁检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fast SARS-CoV-2 virus detection using disposable cartridge strips and a semiconductor-based biosensor platform.

Detection of the SARS-CoV-2 spike protein and inactivated virus was achieved using disposable and biofunctionalized functional strips, which can be connected externally to a reusable printed circuit board for signal amplification with an embedded metal-oxide-semiconductor field-effect transistor (MOSFET). A series of chemical reactions was performed to immobilize both a monoclonal antibody and a polyclonal antibody onto the Au-plated electrode used as the sensing surface. An important step in the biofunctionalization, namely, the formation of Au-plated clusters on the sensor strips, was verified by scanning electron microscopy, as well as electrical measurements, to confirm successful binding of thiol groups on this Au surface. The functionalized sensor was externally connected to the gate electrode of the MOSFET, and synchronous pulses were applied to both the sensing strip and the drain contact of the MOSFET. The resulting changes in the dynamics of drain waveforms were converted into analog voltages and digital readouts, which correlate with the concentration of proteins and virus present in the tested solution. A broad range of protein concentrations from 1 fg/ml to 10 μg/ml and virus concentrations from 100 to 2500 PFU/ml were detectable for the sensor functionalized with both antibodies. The results show the potential of this approach for the development of a portable, low-cost, and disposable cartridge sensor system for point-of-care detection of viral diseases.

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CiteScore
2.70
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