老年性扁豆黑色素细胞和角质形成细胞的三维结构分析

IF 1.8 4区 工程技术
Microscopy Pub Date : 2020-11-01 DOI:10.1093/jmicro/dfaa054
Yuki Mizutani;Mika Yamashita;Rie Hashimoto;Toru Atsugi;Akemi Ryu;Akinobu Hayashi;Yukiko Rikimaru-Nishi;Keisuke Ohta
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引用次数: 6

摘要

老年性雀斑或老年斑是皮肤上的色素沉着斑,通常在长期暴露于紫外线辐射后形成。这种情况是由邻近角质形成细胞内黑色素细胞产生的大量黑色素体(黑色素颗粒)积聚引起的。然而,对于老年性扁豆的黑色素体转移机制,目前还没有达成共识。到目前为止,大多数皮肤的病理学研究都是二维的,并且没有提供关于参与黑色素体转移的黑色素细胞-角质形成细胞网络复杂相互作用的详细数据。我们使用三种不同的显微镜模式对老年性扁豆的表皮微观结构进行了三维重建,以观察拓扑黑素细胞-角质形成细胞的关系和黑素体的分布。共焦激光显微镜图像显示,与正常皮肤相比,老年性雀斑皮肤中的黑素细胞树突突起更频繁地分支和延长。连续的透射电子显微照片显示树突突起延伸到角质形成细胞之间的细胞间隙。聚焦离子束扫描电子显微照片显示,老年扁豆的树突突起包围相邻的角质形成细胞并积累大量黑素体。此外,转移到角质形成细胞的黑素体不仅存在于核上区域,而且存在于除基底侧外的整个核周区域。使用这些不同的显微镜方法有助于阐明老年性扁豆中黑色素细胞和角质形成细胞的三维形态和拓扑结构。我们发现,黑素体在树突过程中定位于受体角质形成细胞周围的区域,有助于老年性扁豆中黑素体的有效转移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Three-dimensional structure analysis of melanocytes and keratinocytes in senile lentigo
Senile lentigo or age spots are hyperpigmented macules of skin that commonly develop following long-term exposure to ultraviolet radiation. This condition is caused by accumulation of large numbers of melanosomes (melanin granules) produced by melanocytes within neighboring keratinocytes. However, there is still no consensus regarding the melanosome transfer mechanism in senile lentigo. To date, most pathohistological studies of skin have been two-dimensional and do not provide detailed data on the complex interactions of the melanocyte–keratinocyte network involved in melanosome transfer. We performed a three-dimensional reconstruction of the epidermal microstructure in senile lentigo using three different microscopic modalities to visualize the topological melanocyte–keratinocyte relationship and melanosome distribution. Confocal laser microscopy images showed that melanocyte dendritic processes are more frequently branched and elongated in senile lentigo skin than in normal skin. Serial transmission electron micrographs showed that dendritic processes extend into intercellular spaces between keratinocytes. Focused ion beam-scanning electron micrographs showed that dendritic processes in senile lentigo encircle adjacent keratinocytes and accumulate large numbers of melanosomes. Moreover, melanosomes transferred to keratinocytes are present not only in the supranuclear area but throughout the perinuclear area except on the basal side. The use of these different microscopic methods helped to elucidate the three-dimensional morphology and topology of melanocytes and keratinocytes in senile lentigo. We show that the localization of melanosomes in dendritic processes to the region encircling recipient keratinocytes contributes to efficient melanosome transfer in senile lentigo.
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来源期刊
Microscopy
Microscopy 工程技术-显微镜技术
自引率
11.10%
发文量
0
审稿时长
>12 weeks
期刊介绍: Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.
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