衣藻作为研究微管蛋白聚谷氨酰化的工具

IF 1.8 4区 工程技术
Microscopy Pub Date : 2019-02-01 DOI:10.1093/jmicro/dfy044
Tomohiro Kubo;Toshiyuki Oda
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引用次数: 3

摘要

α-和β-微管蛋白经过翻译后修饰的聚谷氨酰化。这种修饰对微管的稳定性和静电特性很重要,从而影响各种微管相关蛋白的功能。本文综述并介绍了纤毛/鞭毛微管蛋白的聚谷氨酰化,主要集中在我们利用单细胞绿藻莱茵衣藻的研究上。各种翻译后修饰(PTM)促进了α-和β-微管蛋白的多样性,如乙酰化、酪氨酸化、糖基化、谷氨酰化、磷酸化和甲基化。这些PTM影响微管的稳定性和结构,以及微管和微管相关蛋白(包括分子马达)之间的相互作用。因此,研究微管蛋白PTMs的作用对于理解细胞周期、细胞运动和细胞内运输具有极其重要的意义。Tubulin PTMs于20世纪80年代首次被研究,此后取得了相当大的进展;可能还有其他机制有待阐明。在这里,我们讨论了一种这样的修饰,微管蛋白谷氨酰化,并介绍了我们对单细胞绿藻莱茵衣藻真核鞭毛的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chlamydomonas as a tool to study tubulin polyglutamylation
α- and β-tubulin undergoes polyglutamylation, a post-translational modification. This modification is important for stability and electrostatic properties of microtubules, thereby affecting functions of various microtubule-associated proteins. Here we review and introduce polyglutamylation of ciliary/flagellar tubulin, mainly focusing on our research using the unicellular green alga Chlamydomonas reinhardtii. The diversity of α- and β-tubulin is facilitated by various post-translational modifications (PTMs), such as acetylation, tyrosination, glycylation, glutamylation, phosphorylation and methylation. These PTMs affect the stability and structure of microtubules as well as the interaction between microtubules and microtubule-associated proteins, including molecular motors. Therefore, it is extremely important to investigate the roles of tubulin PTMs for understanding the cell cycle, cell motility and intracellular trafficking. Tubulin PTMs were first studied in the 1980s, and considerable progress has been made since then; it is likely that additional mechanisms remain yet to be elucidated. Here, we discuss one such modification, tubulin glutamylation, and introduce our research on the eukaryotic flagellum of the unicellular green alga Chlamydomonas reinhardtii.
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来源期刊
Microscopy
Microscopy 工程技术-显微镜技术
自引率
11.10%
发文量
0
审稿时长
>12 weeks
期刊介绍: Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.
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