{"title":"STAT3多态性与Survivin、VEGF在中国汉族妇女的相关分析","authors":"Lu Zhao, Hua Yang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the relationship between STAT3 gene polymorphism and missed abortion (MA), and the influence of STAT3 gene polymorphism on the expression of VEGF and survivin.</p><p><strong>Study design: </strong>The missed abortion group included 188 cases of MA. The control group consisted of 200 cases of surgically induced abortion in normal pregnancy. All patients were of Han ethnicity from P.R. China. STAT3 gene from patients' peripheral blood was detected using fluorescent probe real-time quantitative polymerase chain reaction (PCR), which was further analyzed to clarify genotype frequency. Survivin and VEGF mRNA levels in particular genotypes were also detected using qPCR.</p><p><strong>Results: </strong>The STAT3 rs1053004 C/C genotype incidence in the MA group was significantly higher than that in the control group (p<0.05), while the STAT3 rs1053004 T/T and T/C genotypes showed no significant difference between the 2 groups (p>0.05). The STAT3 gene locus rs1053023 genotypes of the 2 groups were not significantly different, either (p>0.05). Furthermore, survivin and VEGF mRNA levels in the peripheral blood of the patients with STAT3 gene loci rs1053004 C/C were significantly decreased as compared to the control group (p<0.05).</p><p><strong>Conclusion: </strong>Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood.</p>","PeriodicalId":50063,"journal":{"name":"生殖医学杂志","volume":"61 11-12","pages":"552-6"},"PeriodicalIF":0.2000,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Correlation Analysis of the STAT3 Polymorphism and Transcription of Survivin and VEGF in Missed Abortion: Experience Among Women of the Chinese Han Population.\",\"authors\":\"Lu Zhao, Hua Yang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the relationship between STAT3 gene polymorphism and missed abortion (MA), and the influence of STAT3 gene polymorphism on the expression of VEGF and survivin.</p><p><strong>Study design: </strong>The missed abortion group included 188 cases of MA. The control group consisted of 200 cases of surgically induced abortion in normal pregnancy. All patients were of Han ethnicity from P.R. China. STAT3 gene from patients' peripheral blood was detected using fluorescent probe real-time quantitative polymerase chain reaction (PCR), which was further analyzed to clarify genotype frequency. Survivin and VEGF mRNA levels in particular genotypes were also detected using qPCR.</p><p><strong>Results: </strong>The STAT3 rs1053004 C/C genotype incidence in the MA group was significantly higher than that in the control group (p<0.05), while the STAT3 rs1053004 T/T and T/C genotypes showed no significant difference between the 2 groups (p>0.05). The STAT3 gene locus rs1053023 genotypes of the 2 groups were not significantly different, either (p>0.05). Furthermore, survivin and VEGF mRNA levels in the peripheral blood of the patients with STAT3 gene loci rs1053004 C/C were significantly decreased as compared to the control group (p<0.05).</p><p><strong>Conclusion: </strong>Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood.</p>\",\"PeriodicalId\":50063,\"journal\":{\"name\":\"生殖医学杂志\",\"volume\":\"61 11-12\",\"pages\":\"552-6\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2016-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"生殖医学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"生殖医学杂志","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
Correlation Analysis of the STAT3 Polymorphism and Transcription of Survivin and VEGF in Missed Abortion: Experience Among Women of the Chinese Han Population.
Objective: To investigate the relationship between STAT3 gene polymorphism and missed abortion (MA), and the influence of STAT3 gene polymorphism on the expression of VEGF and survivin.
Study design: The missed abortion group included 188 cases of MA. The control group consisted of 200 cases of surgically induced abortion in normal pregnancy. All patients were of Han ethnicity from P.R. China. STAT3 gene from patients' peripheral blood was detected using fluorescent probe real-time quantitative polymerase chain reaction (PCR), which was further analyzed to clarify genotype frequency. Survivin and VEGF mRNA levels in particular genotypes were also detected using qPCR.
Results: The STAT3 rs1053004 C/C genotype incidence in the MA group was significantly higher than that in the control group (p<0.05), while the STAT3 rs1053004 T/T and T/C genotypes showed no significant difference between the 2 groups (p>0.05). The STAT3 gene locus rs1053023 genotypes of the 2 groups were not significantly different, either (p>0.05). Furthermore, survivin and VEGF mRNA levels in the peripheral blood of the patients with STAT3 gene loci rs1053004 C/C were significantly decreased as compared to the control group (p<0.05).
Conclusion: Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood.
期刊介绍：
The Journal of Reproductive Medicine® has been the essential tool of Obstetricians and Gynecologists since 1968. As a highly regarded professional journal and the official periodical of six medical associations, JRM® brings timely and relevant information on the latest procedures and advances in the field of reproductive medicine. Published bimonthly, JRM® contains peer-reviewed articles and case reports submitted by top specialists. Common topics include research, clinical practice, and case reports related to general obstetrics and gynecology, infertility, female cancers, gynecologic surgery, contraception, and medical education.
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