{"title":"MYBA1甲基化与“美甲手指”葡萄皮的着色有关","authors":"Hui Xia, Yanqiu Shen, Rongping Hu, Jin Wang, Honghong Deng, Lijin Lin, Xiulan Lv, Qunxian Deng, Kunfu Xu, Dong Liang*","doi":"10.1021/acs.jafc.1c04550","DOIUrl":null,"url":null,"abstract":"<p >The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of <i>DFR</i>, <i>UFGT</i>, and <i>GST</i> were significantly correlated with the anthocyanin content. The promoters of the two <i>VvUFGT</i> alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the <i>VvMYBA1</i> promoter (?1488 to ?1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the <i>VvMYBA1</i> promoter play a crucial role in regulating grape skin coloration.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"69 51","pages":"15649–15659"},"PeriodicalIF":6.2000,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":"{\"title\":\"Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin\",\"authors\":\"Hui Xia, Yanqiu Shen, Rongping Hu, Jin Wang, Honghong Deng, Lijin Lin, Xiulan Lv, Qunxian Deng, Kunfu Xu, Dong Liang*\",\"doi\":\"10.1021/acs.jafc.1c04550\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of <i>DFR</i>, <i>UFGT</i>, and <i>GST</i> were significantly correlated with the anthocyanin content. The promoters of the two <i>VvUFGT</i> alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the <i>VvMYBA1</i> promoter (?1488 to ?1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the <i>VvMYBA1</i> promoter play a crucial role in regulating grape skin coloration.</p>\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":\"69 51\",\"pages\":\"15649–15659\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2021-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jafc.1c04550\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jafc.1c04550","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin
The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of DFR, UFGT, and GST were significantly correlated with the anthocyanin content. The promoters of the two VvUFGT alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the VvMYBA1 promoter (?1488 to ?1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the VvMYBA1 promoter play a crucial role in regulating grape skin coloration.
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The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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