一种新型生物电子传感器中催化发夹组装和杂交链反应级联反应的多重信号放大策略

IF 7.3 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2026-10-14 DOI:10.1021/acs.analchem.6c03093

Junyong Lou,Jiajun Zhan,Futing Wang,Yujin Li,Yifan Lyu,Hongfen Yang,Dan Yang,Ren Cai,Weihong Tan

{"title":"一种新型生物电子传感器中催化发夹组装和杂交链反应级联反应的多重信号放大策略","authors":"Junyong Lou,Jiajun Zhan,Futing Wang,Yujin Li,Yifan Lyu,Hongfen Yang,Dan Yang,Ren Cai,Weihong Tan","doi":"10.1021/acs.analchem.6c03093","DOIUrl":null,"url":null,"abstract":"Abstract A highly sensitive bioelectronic sensor (BES) was developed to analyze miRNA-182, utilizing Ag@Au hexagonal nanoplates (Ag@Au HNPs) in conjunction with catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) cascade reactions. The Ag@Au HNPs improve the conductivity between glucose oxidase (GOD) and carbon paper (CP), thereby contributing to enhanced electron transport in the BES. In addition, the multi-amplification strategy of the BES integrates the CHA-HCR cascade reaction with a capacitor to efficiently amplify the detection signal and enhance the sensitivity. As a result, the BES enables highly sensitive detection of miRNA-182 with a low limit of detection (LOD) of 0.34 fM (S/N = 3) in a wide linear response range of 1∼104 fM. Furthermore, the BES exhibited significant applicability in the detection of miRNA-182 in real samples.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"57 1","pages":""},"PeriodicalIF":7.3000,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Multiple Signal Amplification Strategy from the Cascade Reactions of Catalytic Hairpin Assembly and Hybridization Chain Reaction for a Novel Bioelectronic Sensor\",\"authors\":\"Junyong Lou,Jiajun Zhan,Futing Wang,Yujin Li,Yifan Lyu,Hongfen Yang,Dan Yang,Ren Cai,Weihong Tan\",\"doi\":\"10.1021/acs.analchem.6c03093\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract A highly sensitive bioelectronic sensor (BES) was developed to analyze miRNA-182, utilizing Ag@Au hexagonal nanoplates (Ag@Au HNPs) in conjunction with catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) cascade reactions. The Ag@Au HNPs improve the conductivity between glucose oxidase (GOD) and carbon paper (CP), thereby contributing to enhanced electron transport in the BES. In addition, the multi-amplification strategy of the BES integrates the CHA-HCR cascade reaction with a capacitor to efficiently amplify the detection signal and enhance the sensitivity. As a result, the BES enables highly sensitive detection of miRNA-182 with a low limit of detection (LOD) of 0.34 fM (S/N = 3) in a wide linear response range of 1∼104 fM. Furthermore, the BES exhibited significant applicability in the detection of miRNA-182 in real samples.\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"57 1\",\"pages\":\"\"},\"PeriodicalIF\":7.3000,\"publicationDate\":\"2026-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.analchem.6c03093\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.6c03093","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

摘要

摘要：利用Ag@Au六方纳米片（Ag@Au HNPs）结合催化发夹组装（CHA）和杂交链反应（HCR）级联反应，开发了一种高灵敏度的生物电子传感器（BES）来分析miRNA-182。Ag@Au HNPs提高了葡萄糖氧化酶（GOD）和碳纸（CP）之间的电导率，从而增强了BES中的电子传递。此外，BES的多重放大策略将CHA-HCR级联反应与电容相结合，有效地放大了检测信号，提高了灵敏度。因此，BES能够在1 ~ 104 fM的宽线性响应范围内对miRNA-182进行高灵敏度检测，低检测限（LOD）为0.34 fM （S/N = 3）。此外，BES在实际样品中检测miRNA-182具有显著的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A Multiple Signal Amplification Strategy from the Cascade Reactions of Catalytic Hairpin Assembly and Hybridization Chain Reaction for a Novel Bioelectronic Sensor

Abstract A highly sensitive bioelectronic sensor (BES) was developed to analyze miRNA-182, utilizing Ag@Au hexagonal nanoplates (Ag@Au HNPs) in conjunction with catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) cascade reactions. The Ag@Au HNPs improve the conductivity between glucose oxidase (GOD) and carbon paper (CP), thereby contributing to enhanced electron transport in the BES. In addition, the multi-amplification strategy of the BES integrates the CHA-HCR cascade reaction with a capacitor to efficiently amplify the detection signal and enhance the sensitivity. As a result, the BES enables highly sensitive detection of miRNA-182 with a low limit of detection (LOD) of 0.34 fM (S/N = 3) in a wide linear response range of 1∼104 fM. Furthermore, the BES exhibited significant applicability in the detection of miRNA-182 in real samples.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.